An intrahelical salt bridge within the trigger site stabilizes the GCN4 leucine zipper.


Abstract

We previously reported that a helical trigger segment within the GCN4 leucine zipper monomer is indispensable for the formation of its parallel two-stranded coiled coil. Here, we demonstrate that the intrinsic secondary structure of the trigger site is largely stabilized by an intrahelical salt bridge. Removal of this surface salt bridge by a single amino acid mutation induced only minor changes in the backbone structure of the GCN4 leucine zipper dimer as verified by nuclear magnetic resonance. The mutation, however, substantially destabilized the dimeric structure. These findings support the proposed hierarchic folding mechanism of the GCN4 coiled coil in which local helix formation within the trigger segment precedes dimerization. Study holds ProTherm entries: 10821, 10822, 10823, 10824 Extra Details: helical trigger segment; two-stranded coiled coil; surface salt bridge;,dimeric structure; hierarchic folding mechanism; local helix formation

Submission Details

ID: zv7xz4b63

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:41 p.m.

Version: 1

Publication Details
Kammerer RA;Jaravine VA;Frank S;Schulthess T;Landwehr R;Lustig A;Garcia-Echeverria C;Alexandrescu AT;Engel J;Steinmetz MO,J. Biol. Chem. (2001) An intrahelical salt bridge within the trigger site stabilizes the GCN4 leucine zipper. PMID:11134036
Additional Information

Study Summary

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1CE9 1999-03-18T00:00:00+0000 1.8 HELIX CAPPING IN THE GCN4 LEUCINE ZIPPER
1DGC 1993-07-15T00:00:00+0000 3.0 THE X-RAY STRUCTURE OF THE GCN4-BZIP BOUND TO ATF/CREB SITE DNA SHOWS THE COMPLEX DEPENDS ON DNA FLEXIBILITY
1FAV 2000-07-13T00:00:00+0000 3.0 THE STRUCTURE OF AN HIV-1 SPECIFIC CELL ENTRY INHIBITOR IN COMPLEX WITH THE HIV-1 GP41 TRIMERIC CORE
1FMH 2000-08-17T00:00:00+0000 0 NMR SOLUTION STRUCTURE OF A DESIGNED HETERODIMERIC LEUCINE ZIPPER
1FMH 2000-08-17T00:00:00+0000 0 NMR SOLUTION STRUCTURE OF A DESIGNED HETERODIMERIC LEUCINE ZIPPER
1GCL 1993-10-20T00:00:00+0000 2.1 GCN4 LEUCINE ZIPPER CORE MUTANT P-LI
1GCM 1995-04-25T00:00:00+0000 1.8 GCN4 LEUCINE ZIPPER CORE MUTANT P-LI
1GK6 2001-08-08T00:00:00+0000 1.9 Human vimentin coil 2B fragment linked to GCN4 leucine zipper (Z2B)
1GZL 2002-05-23T00:00:00+0000 1.8 Crystal structure of C14linkmid/IQN17: a cross-linked inhibitor of HIV-1 entry bound to the gp41 hydrophobic pocket
1IHQ 2001-04-19T00:00:00+0000 0 GLYTM1BZIP: A CHIMERIC PEPTIDE MODEL OF THE N-TERMINUS OF A RAT SHORT ALPHA TROPOMYOSIN WITH THE N-TERMINUS ENCODED BY EXON 1B

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 General control protein GCN4 P03069 GCN4_YEAST