Nonaqueous co-solvents, particularly 2,2,2-trifluoroethanol (TFE), have been used as tools to study protein folding. By analyzing FKBP12, an alpha/beta-protein that folds with two-state kinetics, we have been able to address three key questions concerning the use of TFE. First, does TFE perturb the folding pathway? Second, can the observed changes in the rate of folding and unfolding in TFE be attributed to a change in free energy of a single state? Finally, can TFE be used to infer information on secondary structure formation in the transition state? Protein engineering experiments on FKBP12, coupled with folding and unfolding experiments in 0% and 9.6% TFE, conclusively show that TFE does not perturb the folding pathway of this protein. Our results also suggest that the changes in folding and unfolding rates observed in 9.6% TFE are due to a global effect of TFE on the protein, rather than the stabilization of any elements of secondary structure in the transition state. Thus, studies with TFE and other co-solvents can be accurately interpreted only when combined with other techniques. Study holds ProTherm entries: 15054, 15055, 15056, 15057, 15058, 15059, 15060, 15061, 15062, 15063, 15064, 15065, 15066, 15067, 15068, 15069, 15070, 15071, 15072, 15073, 15074, 15075, 15076, 15077, 15078, 15079, 15080, 15081 Extra Details: dithiothreitol (DTT)(1 mM) was added in the experiment,Calculated in 0% TFE using an average mU-F (1.59 } 0.02) trifluoroethanol, protein folding, FKBP12, transition state, secondary structure
ID: zDRNuTyv3
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:45 p.m.
Version: 1
Number of data points | 108 |
Proteins | Peptidyl-prolyl cis-trans isomerase FKBP1A ; Peptidyl-prolyl cis-trans isomerase FKBP1A |
Unique complexes | 13 |
Assays/Quantities/Protocols | Experimental Assay: Cm ; Experimental Assay: m ; Experimental Assay: dG_H2O ; Derived Quantity: ddG_H2O |
Libraries | Mutations for sequence GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE |
Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
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Structure ID | Release Date | Resolution | Structure Title |
---|---|---|---|
1FKK | 1995-08-18T00:00:00+0000 | 2.2 | ATOMIC STRUCTURE OF FKBP12, AN IMMUNOPHILIN BINDING PROTEIN |
1FKL | 1995-08-18T00:00:00+0000 | 1.7 | ATOMIC STRUCTURE OF FKBP12-RAPAYMYCIN, AN IMMUNOPHILIN-IMMUNOSUPPRESSANT COMPLEX |
1TCO | 1996-08-21T00:00:00+0000 | 2.5 | TERNARY COMPLEX OF A CALCINEURIN A FRAGMENT, CALCINEURIN B, FKBP12 AND THE IMMUNOSUPPRESSANT DRUG FK506 (TACROLIMUS) |
3J8H | 2014-10-26T00:00:00+0000 | 3.8 | Structure of the rabbit ryanodine receptor RyR1 in complex with FKBP12 at 3.8 Angstrom resolution |
5GKY | 2016-07-07T00:00:00+0000 | 3.8 | Structure of RyR1 in a closed state (C1 conformer) |
5GKZ | 2016-07-07T00:00:00+0000 | 4.0 | Structure of RyR1 in a closed state (C3 conformer) |
5GL0 | 2016-07-07T00:00:00+0000 | 4.2 | Structure of RyR1 in a closed state (C4 conformer) |
5GL1 | 2016-07-07T00:00:00+0000 | 5.7 | Structure of RyR1 in an open state |
7TZC | 2022-02-15T00:00:00+0000 | 2.45 | A drug and ATP binding site in type 1 ryanodine receptor |
1A7X | 1998-03-18T00:00:00+0000 | 2.0 | FKBP12-FK1012 COMPLEX |
Percent Identity | Matching Chains | Protein | Accession | Entry Name |
---|---|---|---|---|
100.0 | Peptidyl-prolyl cis-trans isomerase FKBP1A | P62942 | FKB1A_HUMAN | |
100.0 | Peptidyl-prolyl cis-trans isomerase FKBP1A | P62943 | FKB1A_RABIT | |
97.2 | Peptidyl-prolyl cis-trans isomerase FKBP1A | P18203 | FKB1A_BOVIN | |
97.2 | Peptidyl-prolyl cis-trans isomerase FKBP1A | P26883 | FKB1A_MOUSE | |
97.2 | Peptidyl-prolyl cis-trans isomerase FKBP1A | Q62658 | FKB1A_RAT |