Viscosity dependence of the folding kinetics of a dimeric and monomeric coiled coil.


Abstract

We measured whether solvent viscosity, and hence chain diffusion, plays a role in the rate-limiting step of the folding reactions of GCN4-p2', a simple alpha-helical coiled coil derived from the leucine zipper region of bZIP transcriptional activator GCN4. To deconvolute the dual effects of viscosogenic solvents on both viscosity, eta, and stability, earlier attempts assumed that the cosolvent and denaturant interact to the same degree in the transition state. Applying this analysis to GCN4-p2' yielded a nearly 1/eta dependence between folding rates and viscosity for both the dimeric and the cross-linked, monomeric versions of the coiled coil, but it revealed no such coherent relationship for cytochrome c. We also developed a method to determine the relative viscosity dependence of the dimeric and monomeric forms of the coiled coil independent of the assumption concerning the transition state's relative interaction with cosolvents and denaturants. Application of this method indicated that the effect of viscosity on both the folding and the unfolding rates was the same for the dimeric and monomeric versions, further supporting the view that the folding of the dimeric version is folding-limited rather than encounter-limited. The finding that GCN4-p2' folding appears to exhibit a 1/eta viscosity dependence implies that the rate-limiting step in folding is opposed predominantly by solvent-derived rather than internal frictional forces. These results are interpreted in relation to various models for protein folding. Study holds ProTherm entries: 16362, 16363, 16364, 16365, 16366, 16367, 16368, 16369, 16370, 16371, 16372, 16373, 16374, 16375, 16376 Extra Details: alpha-helical coiled coil from leucine zipper region, dimeric. equilibrium. GCN4-p2, cytochrome c, folding kinetics, transcriptional activator

Submission Details

ID: zBqP2Xsy3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:47 p.m.

Version: 1

Publication Details
Bhattacharyya RP;Sosnick TR,Biochemistry (1999) Viscosity dependence of the folding kinetics of a dimeric and monomeric coiled coil. PMID:10029555
Additional Information

Study Summary

Number of data points 30
Proteins Cytochrome c ; General control protein GCN4 ; General control protein GCN4 ; Cytochrome c
Unique complexes 2
Assays/Quantities/Protocols Experimental Assay: m buffers:Imidazole: 50 mM, pH:7.0, prot_conc:10-20 micro M, details:Additives Glycerol(25% (w/w)), ; Experimental Assay: dG_H2O buffers:Imidazole: 50 mM, pH:7.0, prot_conc:10-20 micro M, details:Additives Glycerol(25% (w/ ; Experimental Assay: m buffers:Imidazole: 50 mM, pH:7.0, prot_conc:10-20 micro M, details:Additives Glucose(21% (w/w)), ; Experimental Assay: dG_H2O buffers:Imidazole: 50 mM, pH:7.0, prot_conc:10-20 micro M, details:Additives Glucose(21% (w/w ; Experimental Assay: m buffers:Imidazole: 50 mM, details:Additives Ethylene glycol(27% (w/w)),, prot_conc:10-20 micro M, ; Experimental Assay: dG_H2O buffers:Imidazole: 50 mM, details:Additives Ethylene glycol(27% (w/w)),, prot_conc:10-20 micr ; Experimental Assay: m buffers:Imidazole: 50 mM, details:Additives , prot_conc:10-20 micro M, pH:7.0 ; Experimental Assay: dG_H2O buffers:Imidazole: 50 mM, details:Additives , prot_conc:10-20 micro M, pH:7.0 ; Experimental Assay: m prot_conc:34.1 micro M, buffers:Sodium acetate: 20 mM, pH:5.5, details:Additives glycerol (9%), ; Experimental Assay: dG_H2O prot_conc:34.1 micro M, buffers:Sodium acetate: 20 mM, pH:5.5, details:Additives glycerol (9% ; Experimental Assay: m buffers:Sodium acetate: 20 mM, pH:5.5, prot_conc:2-10 micro M, details:Additives glycerol (15.2%), ; Experimental Assay: dG_H2O buffers:Sodium acetate: 20 mM, pH:5.5, prot_conc:2-10 micro M, details:Additives glycerol (15 ; Experimental Assay: m prot_conc:34.1 micro M, buffers:Sodium acetate: 20 mM, pH:5.5, details:Additives glycerol (15.2%), ; Experimental Assay: dG_H2O prot_conc:34.1 micro M, buffers:Sodium acetate: 20 mM, pH:5.5, details:Additives glycerol (15 ; Experimental Assay: m buffers:Sodium acetate: 20 mM, pH:5.5, prot_conc:2-10 micro M, details:Additives glycerol (9%), ; Experimental Assay: dG_H2O buffers:Sodium acetate: 20 mM, pH:5.5, prot_conc:2-10 micro M, details:Additives glycerol (9% ; Experimental Assay: m buffers:Sodium acetate: 20 mM, pH:5.5, details:Additives , prot_conc:2-10 micro M ; Experimental Assay: dG_H2O buffers:Sodium acetate: 20 mM, pH:5.5, details:Additives , prot_conc:2-10 micro M ; Experimental Assay: m prot_conc:34.1 micro M, details:Additives , buffers:Sodium acetate: 20 mM, pH:5.5 ; Experimental Assay: dG_H2O prot_conc:34.1 micro M, details:Additives , buffers:Sodium acetate: 20 mM, pH:5.5
Libraries Mutations for sequence GDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFTYTDANKNKGITWKEETLMEYLENPKKYIPGTKMIFAGIKKKTEREDLIAYLKKATNE ; Mutations for sequence RMKQLEDKVEELLSKNYHLENEVARLKKLVGER

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1OCD 1997-06-16 CYTOCHROME C (OXIDIZED) FROM EQUUS CABALLUS, NMR, MINIMIZED AVERAGE STRUCTURE
5IIV 2017-03-15 GCN4p pH 1.5
2N9B 2016-06-15 Solution NMR Structure of Antiparallel Myosin-10:GCN4 Tandem Coiled-Coil
1FMH 2000-11-01 NMR SOLUTION STRUCTURE OF A DESIGNED HETERODIMERIC LEUCINE ZIPPER
1M60 2002-08-07 Solution Structure of Zinc-substituted cytochrome c
2K8X 2009-04-28 GlyTM1b(1-19)zip: A Chimeric Peptide Model of the N-Terminus of a Rat Short Alpha-Tropomyosin with the N-Terminus Encoded by Exon 1b in Complex with TM9d(252-284), a Peptide Model Containing the C Terminus of Alpha-Tropomyosin Encoded by Exon 9d
5IEW 2017-03-15 NMR Structures Show Unwinding of the GCN4p Coiled Coil Superhelix Accompanying Disruption of Ion Pairs at Acidic pH
1AKK 1997-09-17 SOLUTION STRUCTURE OF OXIDIZED HORSE HEART CYTOCHROME C, NMR, MINIMIZED AVERAGE STRUCTURE
1FI9 2000-08-23 SOLUTION STRUCTURE OF THE IMIDAZOLE COMPLEX OF CYTOCHROME C
2FRC 1997-07-29 CYTOCHROME C (REDUCED) FROM EQUUS CABALLUS, NMR, MINIMIZED AVERAGE STRUCTURE
5IIR 2017-03-15 NMR Structures Show Unwinding of the GCN4p Coiled Coil Superhelix Accompanying Disruption of Ion Pairs at Acidic pH
1IHQ 2001-10-03 GLYTM1BZIP: A CHIMERIC PEPTIDE MODEL OF THE N-TERMINUS OF A RAT SHORT ALPHA TROPOMYOSIN WITH THE N-TERMINUS ENCODED BY EXON 1B
2GIW 1998-12-09 SOLUTION STRUCTURE OF REDUCED HORSE HEART CYTOCHROME C, NMR, 40 STRUCTURES
2OVN 2007-04-03 NMR structure of the GCN4 trigger peptide
1LC1 2003-06-03 Solution Structure Of Reduced Horse Heart Cytochrome c in 30% Acetonitrile Solution, NMR Minimized Average Structure
2G9J 2006-11-07 Complex of TM1a(1-14)Zip with TM9a(251-284): a model for the polymerization domain ('overlap region') of tropomyosin, Northeast Structural Genomics Target OR9
2N3B 2015-10-28 Structure of oxidized horse heart cytochrome c encapsulated in reverse micelles
5ZKV 2019-05-22 Solution structure of molten globule state of L94G mutant of horse cytochrome-c
2LPB 2012-02-22 Structure of the complex of the central activation domain of Gcn4 bound to the mediator co-activator domain 1 of Gal11/med15
1I5T 2001-03-21 SOLUTION STRUCTURE OF CYANOFERRICYTOCHROME C
1GIW 1998-12-09 SOLUTION STRUCTURE OF REDUCED HORSE HEART CYTOCHROME C, NMR, MINIMIZED AVERAGE STRUCTURE
1ZTA 1993-04-15 THE SOLUTION STRUCTURE OF A LEUCINE-ZIPPER MOTIF PEPTIDE
1FI7 2000-08-23 Solution structure of the imidazole complex of cytochrome C
1LC2 2003-06-03 Solution Structure Of Reduced Horse Heart Cytochrome c in 30% Acetonitrile Solution, NMR 30 Structures
1TMZ 1998-06-17 TMZIP: A CHIMERIC PEPTIDE MODEL OF THE N-TERMINUS OF ALPHA TROPOMYOSIN, NMR, 15 STRUCTURES
3AZD 2011-10-19 0.98 Crystal structure of tropomyosin N-terminal fragment at 0.98A resolution
2WQ1 2009-11-03 1.08 GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating bromide
2WQ0 2009-11-03 1.12 GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating chloride
5C0Z 2016-09-21 1.12 The structure of oxidized rat cytochrome c at 1.13 angstroms resolution
4OWI 2014-05-21 1.2 peptide structure
2WQ3 2009-11-03 1.22 GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating chloride and nitrate
2WPZ 2009-11-03 1.25 GCN4 leucine zipper mutant with two VxxNxxx motifs coordinating chloride
2HY6 2006-10-24 1.25 A seven-helix coiled coil
5DF5 2016-09-14 1.3 The structure of oxidized rat cytochrome c (T28E) at 1.30 angstroms resolution.
2IPZ 2007-01-16 1.35 A Parallel Coiled-Coil Tetramer with Offset Helices
5APU 2016-01-27 1.35 Sequence IANKEDKAD inserted between GCN4 adaptors - Structure A9b black
2YNY 2012-12-12 1.35 Salmonella enterica SadA 255-302 fused to GCN4 adaptors (SadAK1)
2WQ2 2009-11-03 1.36 GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating iodide
5C9M 2016-09-21 1.36 The structure of oxidized rat cytochrome c (T28A) at 1.362 angstroms resolution.
5APS 2016-01-27 1.37 Sequence IENKKAD inserted between GCN4 adaptors - Structure A7
2YNZ 2012-12-12 1.4 Salmonella enterica SadA 823-947 fused to a GCN4 adaptor (SadAK5)
2NRN 2007-04-17 1.4 Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant
3M48 2011-02-16 1.45 GCN4 Leucine Zipper Peptide Mutant
1LLM 2003-09-30 1.5 Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA
2B4Z 2005-10-11 1.5 Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.
2BNI 2005-03-30 1.5 pLI mutant E20C L16G Y17H, antiparallel
2B1F 2006-01-31 1.5 Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat
5KHT 2017-06-21 1.5 Crystal structure of the N-terminal fragment of tropomyosin isoform Tpm1.1 at 1.5 A resolution
3I1G 2009-07-07 1.6 Crystal structure of a GCN4 leucine zipper mutant at 1.6 A resolution
5APZ 2016-01-27 1.6 Thermosinus carboxydivorans Nor1 Tcar0761 residues 68-101 and 191-211 fused to GCN4 adaptors
2CCN 2006-04-06 1.6 pLI E20C is antiparallel
5APW 2016-01-27 1.6 Sequence MATKDD inserted between GCN4 adaptors - Structure T6
1IJ2 2001-08-08 1.7 GCN4-pVTL Coiled-coil Trimer with Threonine at the a(16) position
3CK4 2008-10-07 1.7 A heterospecific leucine zipper tetramer
2CCF 2006-04-06 1.7 Antiparallel Configuration of pLI E20S
3CRP 2008-10-07 1.7 A heterospecific leucine zipper tetramer
1UO4 2004-10-13 1.7 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
6FF5 2018-03-21 1.74 X-ray structure of bovine heart cytochrome c at high ionic strength
3O1Y 2012-01-25 1.75 Electron transfer complexes: Experimental mapping of the redox-dependent cytochrome c electrostatic surface
2WPY 2009-11-03 1.75 GCN4 leucine zipper mutant with one VxxNxxx motif coordinating chloride
1IJ3 2001-08-08 1.8 GCN4-pVSL Coiled-coil trimer with Serine at the a(16) position
1GCM 1996-01-29 1.8 GCN4 LEUCINE ZIPPER CORE MUTANT P-LI
2ZTA 1992-10-15 1.8 X-RAY STRUCTURE OF THE GCN4 LEUCINE ZIPPER, A TWO-STRANDED, PARALLEL COILED COIL
5APT 2016-01-27 1.8 Sequence IENKADKAD inserted between GCN4 adaptors - Structure A9
1WEJ 1998-12-09 1.8 IGG1 FAB FRAGMENT (OF E8 ANTIBODY) COMPLEXED WITH HORSE CYTOCHROME C AT 1.8 A RESOLUTION
1PIQ 1998-09-30 1.8 CRYSTAL STRUCTURE OF GCN4-PIQ, A TRIMERIC COILED COIL WITH BURIED POLAR RESIDUES
1CE9 1999-03-25 1.8 HELIX CAPPING IN THE GCN4 LEUCINE ZIPPER
5APX 2016-01-27 1.8 Sequence MATKDDIAN inserted between GCN4 adaptors - Structure T9(6)
6O2F 2019-06-26 1.8 GCN4 with NPEG4 at position 18
1ZII 1997-07-07 1.8 GCN4-LEUCINE ZIPPER CORE MUTANT ASN16ABA IN THE DIMERIC STATE
3WC8 2013-12-11 1.8 Dimeric horse cytochrome c obtained by refolding with desalting method
1ZIK 1997-07-07 1.8 GCN4-LEUCINE ZIPPER CORE MUTANT ASN16LYS IN THE DIMERIC STATE
3WUI 2014-07-16 1.8 Dimeric horse cytochrome c formed by refolding from molten globule state
2EFR 2008-03-04 1.8 Crystal structure of the c-terminal tropomyosin fragment with N- and C-terminal extensions of the leucine zipper at 1.8 angstroms resolution
4TL1 2014-08-06 1.8 GCN4-p1 with mutation to 1-Aminocyclohexanecarboxylic acid at residue 10
2VNL 2009-02-10 1.8 MUTANT Y108Wdel OF THE HEADBINDING DOMAIN OF PHAGE P22 TAILSPIKE C- TERMINally fused to ISOLEUCINE ZIPPER pIIGCN4 (chimera II)
1GZL 2002-10-10 1.8 Crystal structure of C14linkmid/IQN17: a cross-linked inhibitor of HIV-1 entry bound to the gp41 hydrophobic pocket
3ZMF 2013-02-20 1.85 Salmonella enterica SadA 303-358 fused to GCN4 adaptors (SadAK2)
2O7H 2007-11-27 1.86 Crystal structure of trimeric coiled coil GCN4 leucine zipper
1IJ1 2001-08-08 1.86 GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position
1IJ0 2001-08-08 1.86 Coiled Coil Trimer GCN4-pVLS Ser at Buried D Position
4NJ0 2014-08-20 1.9 GCN4-p1 single Val9 to Ile mutant
1RB6 2004-01-13 1.9 ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TETRAGONAL FORM
4G2K 2012-09-12 1.9 Crystal structure of the Marburg Virus GP2 ectodomain in its post-fusion conformation
3O20 2012-01-25 1.9 Electron transfer complexes:experimental mapping of the Redox-dependent Cytochrome C electrostatic surface
1HRC 1994-11-01 1.9 HIGH-RESOLUTION THREE-DIMENSIONAL STRUCTURE OF HORSE HEART CYTOCHROME C
6O2E 2019-06-26 1.9 GCN4 with asparagine at position 18
1RB4 2004-01-13 1.9 ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TETRAGONAL AUTOMATIC SOLUTION
2CCE 2006-04-06 1.9 Parallel Configuration of pLI E20S
1RB5 2004-01-13 1.9 ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TRIGONAL FORM
3K7Z 2009-11-24 1.9 GCN4-Leucine zipper core mutant as N16A trigonal automatic solution
1GK6 2002-03-15 1.9 Human vimentin coil 2B fragment linked to GCN4 leucine zipper (Z2B)
1UO3 2004-10-13 1.92 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
1W5K 2004-09-24 1.92 AN ANTI-PARALLEL FOUR HELIX BUNDLE
6E52 2019-02-27 1.93 Chimeric structure of Saccharomyces cerevisiae GCN4 leucine zipper fused to Staphylococcus aureus AgrC cytoplasmic histidine kinase module (dataset anisotropically truncated by STARANISO)
3I5C 2009-08-18 1.94 Crystal structure of a fusion protein containing the leucine zipper of GCN4 and the GGDEF domain of WspR from Pseudomonas aeruginosa
4C46 2013-09-11 1.95 ANDREI-N-LVPAS fused to GCN4 adaptors
1UO2 2004-10-13 1.99 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
1LD4 2002-11-04 11.4 Placement of the Structural Proteins in Sindbis Virus
2YBB 2011-10-19 19.0 Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876)
2B22 2006-01-31 2.0 Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat
4NJ1 2014-08-20 2.0 GCN4-p1 double Val9, 23 to Ile mutant
4NIZ 2014-08-20 2.0 GCN4-p1 single Val9 to aminobutyric acid mutant
4DMD 2012-11-14 2.0 GCN4 leucine zipper domain in a dimeric oligomerization state
2YO2 2012-12-12 2.0 Salmonella enterica SadA 255-358 fused to GCN4 adaptors (SadAK12)
2EFS 2008-03-04 2.0 Crystal structure of the C-terminal tropomyosin fragment with N- and C-terminal extensions of the leucine zipper at 2.0 angstroms resolution
5IY5 2017-01-11 2.0 Electron transfer complex of cytochrome c and cytochrome c oxidase at 2.0 angstrom resolution
3G9R 2009-05-12 2.0 Structure of the HIV-1 gp41 Membrane-Proximal Ectodomain Region in a Putative Prefusion Conformation
5APV 2016-01-27 2.0 Sequence IANKEDKAD inserted between GCN4 adaptors - Structure A9b grey
5APY 2016-01-27 2.0 Sequence MATKDDIAN inserted between GCN4 adaptors - Structure T9(9)
2AHP 2006-08-01 2.0 GCN4 leucine zipper, mutation of Lys15 to epsilon-azido-Lys
1ZIJ 1997-07-07 2.0 GCN4-LEUCINE ZIPPER CORE MUTANT ASN16ABA IN THE TRIMERIC STATE
1ZIM 1997-07-07 2.0 GCN4-LEUCINE ZIPPER CORE MUTANT ASN16GLN IN THE TRIMERIC STATE
3GJP 2010-03-09 2.0 Crystal structure of mutant coiled coil GCN4 leucine zipper
2YO3 2012-12-12 2.0 Salmonella enterica SadA 1185-1386 fused to GCN4 adaptors (SadAK14)
2VKY 2009-02-10 2.05 Headbinding Domain of Phage P22 Tailspike C-Terminally Fused to Isoleucine Zipper pIIGCN4 (Chimera I)
1UNU 2004-10-13 2.07 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
1UNT 2004-10-13 2.07 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
1UO5 2004-10-13 2.07 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
1CRC 1996-03-08 2.08 CYTOCHROME C AT LOW IONIC STRENGTH
3NBT 2010-07-14 2.1 Crystal structure of trimeric cytochrome c from horse heart
1GCL 1995-06-03 2.1 GCN4 LEUCINE ZIPPER CORE MUTANT P-LI
2Z5I 2008-04-22 2.1 Crystal structure of the head-to-tail junction of tropomyosin
5APQ 2016-01-27 2.1 Sequence IENKAD inserted between GCN4 adaptors - Structure A6
2WG5 2009-04-28 2.1 Proteasome-Activating Nucleotidase (PAN) N-domain (57-134) from Archaeoglobus fulgidus fused to GCN4
6H9M 2019-02-13 2.1 Coiled-coil domain-containing protein 90B residues 43-125 from Homo sapiens fused to a GCN4 adaptor
4NFG 2014-09-24 2.11 K13R mutant of horse cytochrome c and yeast cytochrome c peroxidase complex
1UNV 2004-10-13 2.14 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
1W5G 2004-09-24 2.16 An anti-parallel four helix bundle (acetimide modification).
1W5L 2004-09-24 2.17 An anti-parallel to parallel switch.
4RSZ 2015-01-14 2.19 The X-ray structure of the Primary Adduct formed in the Reaction between Cisplatin and Cytochrome c
1UNW 2004-10-13 2.2 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
1W5J 2004-09-24 2.2 AN ANTI-PARALLEL FOUR HELIX BUNDLE
3NBS 2010-07-14 2.2 Crystal structure of dimeric cytochrome c from horse heart
4NJ2 2014-08-20 2.2 GCN4-p1 triple Val9, 23,30 to Ile mutant
2DGC 1996-03-08 2.2 GCN4 BASIC DOMAIN, LEUCINE ZIPPER COMPLEXED WITH ATF/CREB SITE DNA
6E95 2019-02-27 2.25 Chimeric structure of Saccharomyces cerevisiae GCN4 leucine zipper fused to Staphylococcus aureus AgrC cytoplasmic histidine kinase module (dataset isotropically truncated by HKL2000)
1ZIL 1997-07-07 2.25 GCN4-LEUCINE ZIPPER CORE MUTANT ASN16GLN IN THE DIMERIC STATE
3BAT 2008-01-08 2.3 Crystal structure of the N-terminal region of the scallop myosin rod, monoclinic (P21) form
1UNY 2004-10-13 2.3 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
4HU5 2013-08-21 2.3 Oxime side-chain cross-links in the GCN4-p1 dimeric coiled coil: Linear precursor
3BAS 2008-01-08 2.3 Crystal structure of the N-terminal region of the scallop myosin rod, monoclinic (C2) form
4HU6 2013-08-21 2.3 Oxime side-chain cross-links in the GCN4-p1 dimeric coiled coil: Cyclic product
1W5I 2004-09-24 2.3 ABA does not affect topology of pLI.
1UNZ 2004-10-13 2.3 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
5APP 2016-01-27 2.3 Actinobacillus actinomycetemcomitans OMP100 residues 133-198 fused to GCN4 adaptors
1KTD 2002-05-01 2.4 CRYSTAL STRUCTURE OF CLASS II MHC MOLECULE IEK BOUND TO PIGEON CYTOCHROME C PEPTIDE
1UNX 2004-10-13 2.4 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
1UO0 2004-10-13 2.4 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
2WG6 2009-04-28 2.5 Proteasome-Activating Nucleotidase (PAN) N-domain (57-134) from Archaeoglobus fulgidus fused to GCN4, P61A Mutant
1W5H 2004-09-24 2.5 An anti-parallel four helix bundle.
1NKN 2003-07-22 2.5 VISUALIZING AN UNSTABLE COILED COIL: THE CRYSTAL STRUCTURE OF AN N-TERMINAL SEGMENT OF THE SCALLOP MYOSIN ROD
1UO1 2004-10-13 2.5 Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
1U75 2004-09-28 2.55 Electron Transfer Complex between Horse Heart Cytochrome c and Zinc-Porphyrin Substituted Cytochrome c Peroxidase
2D3E 2006-09-12 2.6 Crystal structure of the C-Terminal fragment of rabbit skeletal alpha-tropomyosin
1SWI 1996-11-08 2.6 GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE
1KQL 2002-05-29 2.7 Crystal structure of the C-terminal region of striated muscle alpha-tropomyosin at 2.7 angstrom resolution
2YO0 2012-12-12 2.8 Salmonella enterica SadA 1049-1304 fused to GCN4 adaptors (SadAK9-cfI)
2PCB 1993-07-15 2.8 CRYSTAL STRUCTURE OF A COMPLEX BETWEEN ELECTRON TRANSFER PARTNERS, CYTOCHROME C PEROXIDASE AND CYTOCHROME C
2Z5H 2008-04-22 2.89 Crystal structure of the head-to-tail junction of tropomyosin complexed with a fragment of TnT
1YSA 1993-10-31 2.9 THE GCN4 BASIC REGION LEUCINE ZIPPER BINDS DNA AS A DIMER OF UNINTERRUPTED ALPHA HELICES: CRYSTAL STRUCTURE OF THE PROTEIN-DNA COMPLEX
3P8M 2011-08-31 2.9 Human dynein light chain (DYNLL2) in complex with an in vitro evolved peptide dimerized by leucine zipper
1FAV 2000-08-23 3.0 THE STRUCTURE OF AN HIV-1 SPECIFIC CELL ENTRY INHIBITOR IN COMPLEX WITH THE HIV-1 GP41 TRIMERIC CORE
1DGC 1994-06-22 3.0 THE X-RAY STRUCTURE OF THE GCN4-BZIP BOUND TO ATF/CREB SITE DNA SHOWS THE COMPLEX DEPENDS ON DNA FLEXIBILITY
2YO1 2012-12-12 3.1 Salmonella enterica SadA 1049-1304 fused to GCN4 adaptors (SadAK9- cfII)
6HN5 2018-11-21 3.2 Leucine-zippered human insulin receptor ectodomain with single bound insulin - 'upper' membrane-distal part
3JBT 2015-11-18 3.8 Atomic structure of the Apaf-1 apoptosome
5JUY 2016-10-19 4.1 Active human apoptosome with procaspase-9
6HN4 2018-11-21 4.2 Leucine-zippered human insulin receptor ectodomain with single bound insulin - 'lower' membrane-proximal part
5WVE 2017-02-08 4.4 Apaf-1-Caspase-9 holoenzyme
3J2T 2013-04-10 9.5 An improved model of the human apoptosome

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
91.3 Cytochrome c P00021 CYC_COLLI
92.2 Cytochrome c P00020 CYC_ANAPL
92.3 Cytochrome c P81280 CYC_ALLMI
90.5 Cytochrome c Q52V10 CYC_SAISC
93.3 Cytochrome c P00012 CYC_MIRLE
93.3 Cytochrome c Q52V09 CYC_CEPBA
93.3 Cytochrome c P00013 CYC_MINSC
93.3 Cytochrome c P00014 CYC_MACGI
94.3 Cytochrome c P00011 CYC_CANLF
94.3 Cytochrome c P62898 CYC_RAT
94.3 Cytochrome c P00008 CYC_RABIT
94.3 Cytochrome c P62897 CYC_MOUSE
95.2 Cytochrome c P68098 CYC_LAMGU
95.2 Cytochrome c P68100 CYC_ESCRO
95.2 Cytochrome c P68099 CYC_CAMDR
94.3 Cytochrome c P00007 CYC_HIPAM
97.1 Cytochrome c P62896 CYC_SHEEP
97.1 Cytochrome c P62895 CYC_PIG
97.1 Cytochrome c P62894 CYC_BOVIN
99.0 Cytochrome c P68096 CYC_EQUBU
99.0 Cytochrome c P68097 CYC_EQUAS
100.0 Cytochrome c P00004 CYC_HORSE
100.0 General control protein GCN4 P03069 GCN4_YEAST
90.3 Cytochrome c B4USV4 CYC_OTOGA