The peculiar nature of the guanidine hydrochloride-induced two-state denaturation of staphylococcal nuclease: a calorimetric study.


Abstract

This work determines the ratio of DeltaH(vH) /DeltaH(cal) for staphylococcal nuclease (SN) denaturation in guanidine hydrochloride (GdnHCl) to test whether GdnHCl-induced denaturation is two-state. Heats of mixing of SN as a function of [GdnHCl] were determined at pH 7.0 and 25 degrees C. The resulting plot of DeltaH(mix) vs [GdnHCl] exhibits a sigmoid shaped curve with linear pre- and post-denaturational base lines. Extending the pre- and post-denaturational lines to zero [GdnHCl] gives a calorimetric DeltaH (DeltaH(cal)) of 24.1 +/- 1.0 kcal/mol, for SN denaturation in the limit of zero GdnHCl concentration. Guanidine hydrochloride-induced denaturation Gibbs energy changes in the limit of zero denaturant concentration (DeltaG degrees (N)(-)(D)) at pH 7. 0 were determined for SN from fluorescence measurements at fixed temperatures over the range from 15 to 35 degrees C. Analysis of the resulting temperature-dependent DeltaG degrees (N)(-)(D) data defines a van't Hoff denaturation enthalpy change (DeltaH(vH)) of 26. 4 +/- 2.8 kcal/mol. The model-dependent van't Hoff DeltaH(vH) divided by the model-independent DeltaH(cal) gives a ratio of 1.1 +/- 0.1 for DeltaH(vH)/DeltaH(cal), a result that rules out the presence of thermodynamically important intermediate states in the GdnHCl-induced denaturation of SN. The likelihood that GdnHCl-induced SN denaturation involves a special type of two-state denaturation, known as a variable two-state process, is discussed in terms of the thermodynamic implications of the process. Study holds ProTherm entries: 6547, 6548, 6549, 6550, 6551, 6552, 6553 Extra Details: hydrochloride-induced denaturation; temperature-dependent;,enthalpy change; two-state denaturation

Submission Details

ID: yQn47vJt

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:32 p.m.

Version: 1

Publication Details
Yang M;Liu D;Bolen DW,Biochemistry (1999) The peculiar nature of the guanidine hydrochloride-induced two-state denaturation of staphylococcal nuclease: a calorimetric study. PMID:10460179
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1JOR 2001-08-22 Ensemble structures for unligated Staphylococcal nuclease-H124L
2SOB 1995-12-07 SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES
2PQE 2007-06-19 Solution structure of proline-free mutant of staphylococcal nuclease
2M00 2013-03-06 Solution structure of staphylococcal nuclease E43S mutant in the presence of ssDNA and Cd2+
2FXZ 2006-12-19 Solution structure of 97-109 segment of staphylococcal nuclease
1JOQ 2001-08-22 Ensemble structures for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate
2LKV 2012-09-12 Staphylococcal Nuclease PHS variant
1JOO 2001-08-22 Averaged structure for unligated Staphylococcal nuclease-H124L
1JOK 2001-08-22 Averaged structure for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate
2KHS 2009-10-20 Solution structure of SNase121:SNase(111-143) complex
4HMJ 2012-10-31 1.35 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D at cryogenic temperature
4EQP 2012-05-02 1.35 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D at cryogenic temperature
3SXH 2011-07-27 1.4 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92AL103A at cryogenic temperature
4KY5 2013-06-12 1.4 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D at pH 7 and cryogenic temperature
3TME 2011-09-14 1.4 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E at cryogenic temperature
4N9P 2013-12-04 1.4 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L25A/V99T at cryogenic temperature
4DFA 2012-02-01 1.4 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L36A at cryogenic temperature
3SR1 2011-07-27 1.45 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature
5JOB 2016-05-11 1.45 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92A at cryogenic temperature
4DGZ 2012-02-08 1.47 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L125A at cryogenic temperature
3SHL 2011-06-29 1.48 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74KL25A at cryogenic temperature
3LX0 2011-02-16 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N at cryogenic temperature
4DF7 2012-02-01 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23L/V99I at cryogenic temperature
5DAU 2015-09-02 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V39E/V104E at cryogenic temperature
2SNS 1982-07-29 1.5 STAPHYLOCOCCAL NUCLEASE. PROPOSED MECHANISM OF ACTION BASED ON STRUCTURE OF ENZYME-THYMIDINE 3(PRIME),5(PRIME)-BIPHOSPHATE-CALCIUM ION COMPLEX AT 1.5-ANGSTROMS RESOLUTION
4NP5 2013-12-04 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92N at cryogenic temperature
5HUR 2016-02-10 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25T/I92K at cryogenic temperature
5KEE 2016-06-22 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92F at cryogenic temperature
3MEH 2011-03-16 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A at cryogenic temperature
4NDX 2013-11-13 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N at cryogenic temperature
4KV6 2013-06-12 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS R126Q at cryogenic temperature
4K2L 2013-04-24 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92Q at cryogenic temperature
4KY7 2013-06-12 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74D at cryogenic temperature
4KJN 2013-05-15 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66A/V99T at cryogenic temperature
4KD4 2013-05-08 1.55 Crystal structure of Staphylococcal nuclease variant V23L/L25V/V66I/I72V at cryogenic temperature
3MHB 2011-03-23 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS L38A at cryogenic temperature
3VA5 2012-01-25 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V23A at cryogenic temperature
3RUZ 2011-06-15 1.58 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74K at cryogenic temperature
3MZ5 2011-03-23 1.58 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103A at cryogenic temperature
5CV8 2015-08-12 1.58 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/T62H at cryogenic temperature
4LAA 2013-08-21 1.58 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36H at cryogenic temperature
4H7B 2012-10-17 1.6 Crystal Structure of Staphylococcal nuclease mutant I72V/V99L
3PMF 2011-11-16 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23A at cryogenic temperature
4IAL 2013-01-09 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS H121E at cryogenic temperature
1EY4 2000-10-18 1.6 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S59A
4J6H 2013-03-06 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS H121Q at cryogenic temperature
1EY0 2000-10-18 1.6 STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.6 A RESOLUTION
4F8M 2012-06-13 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23I/I92V at cryogenic temperature
3OSO 2011-09-21 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A at cryogenic temperature
3TP8 2011-09-21 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E at cryogenic temperature
4K14 2013-04-17 1.6 Crystal Structure of Staphylococcal nuclease mutant V66I/V99L
4NKL 2013-11-20 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92Q at cryogenic temperature
4QB4 2014-06-11 1.6 Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L
3QOJ 2011-03-16 1.6 Cryogenic structure of Staphylococcal nuclease variant D+PHS/V23K
5IOD 2016-03-23 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99E at cryogenic temperature
4IUN 2013-02-06 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS R126E at cryogenic temperature
4TRD 2014-07-16 1.6 Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K/V74K at pH 9.0 at cryogenic temperature
4N9T 2013-10-30 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92S at cryogenic temperature
4WOR 2014-10-29 1.6 Staphylococcal nuclease in complex with Ca2+ and thymidine-3'-5'-diphosphate (pdTp) at room temperature
4KHV 2013-05-15 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S at cryogenic temperature
5KRU 2016-07-27 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66K at cryogenic temperature
4I65 2013-03-20 1.61 Crystal Structure of Staphylococcal nuclease mutant V23I/L25V/I72V/I92V
3MXP 2011-03-23 1.61 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature
5CV9 2015-08-12 1.62 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/V66H at cryogenic temperature
5C4Z 2015-07-15 1.62 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25H at cryogenic temperature
4DU9 2012-03-07 1.63 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V74A at cryogenic temperature
3QB3 2012-01-18 1.63 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92KL25A at cryogenic temperature
4S3S 2015-07-01 1.64 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K/V23A at cryogenic temperature
4YIJ 2015-03-18 1.64 Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature
3NK9 2011-06-29 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74A at cryogenic temperature
4PMC 2014-06-11 1.65 Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K V74K at cryogenic temperature
4R8N 2014-09-17 1.65 Crystal structure of Staphylococcal nuclease variant V23I/V66I/I72V/I92V at cryogenic temperature
4K6D 2013-04-24 1.65 Crystal structure of Staphylococcal nuclease variant V23M/T62F at cryogenic temperature
2EY6 2006-10-24 1.65 Crystal structure of Staphylococcal nuclease mutant T41V
6B8R 2017-10-18 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36Q at cryogenic temperature
4K5W 2013-04-24 1.65 Crystal structure of Staphylococcal nuclease variant V23M/L25F/T62F at cryogenic temperature
1SNC 1990-10-15 1.65 THE CRYSTAL STRUCTURE OF THE TERNARY COMPLEX OF STAPHYLOCOCCAL NUCLEASE, CA2+, AND THE INHIBITOR PD*TP, REFINED AT 1.65 ANGSTROMS
4WRD 2015-07-08 1.65 Crystal structure of Staphylcoccal nulease variant Delta+PHS V66E L125E at cryogenic temperature
3NXW 2011-07-20 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS L125A at cryogenic temperature
4K5X 2013-04-24 1.65 Crystal structure of Staphylococcal nuclease variant V23M/L36F at cryogenic temperature
5CV7 2015-08-12 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92D at cryogenic temperature
1IHZ 2003-06-17 1.65 Structure of S. nuclease mutant quintuple mutant V23L/V66L/I72L/I92L/V99L
4J1M 2013-02-20 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS R105E at cryogenic temperature
4K2K 2013-04-24 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/L36A/I92A at cryogenic temperature
4RKL 2014-11-26 1.66 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66T at cryogenic temperature
4MIU 2013-10-02 1.67 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V99T at cryogenic temperature
4OL7 2015-01-28 1.67 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E A109E at cryogenic temperature
1STY 1993-04-15 1.67 THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE
4PNY 2014-03-12 1.68 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72H at cryogenic temperature
4KY6 2013-06-12 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25D at pH 6 and cryogenic temperature
5DEH 2015-09-02 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS N100D at cryogenic temperature
2F0T 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant V66L/V99I
1EYD 2000-10-18 1.7 STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.7 A RESOLUTION
3H6M 2010-03-09 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E at cryogenic temperature
4NMZ 2013-12-11 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23Q/V66A at cryogenic temperature
4ZUI 2015-05-27 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23H at cryogenic temperature
1STA 1994-06-22 1.7 ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE
5C5M 2015-07-08 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D / V104E at cryogenic temperature
1SNQ 1997-02-12 1.7 PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE
5IGG 2016-03-09 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I72E at cryogenic temperature
2EY2 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant T41C
2EYL 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant T82S
5F2D 2015-12-16 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N/V99T at cryogenic temperature
1STG 1995-01-26 1.7 TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES
5IGF 2016-03-09 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V66D at cryogenic temperature
2F0N 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant L25I/I72L
3NP8 2011-06-29 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A at cryogenic temperature
2EYO 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant T120S
1SNO 1997-02-12 1.7 PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE
1EY5 2000-10-18 1.7 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V
1STN 1994-07-31 1.7 THE CRYSTAL STRUCTURE OF STAPHYLOCOCCAL NUCLEASE REFINED AT 1.7 ANGSTROMS RESOLUTION
4IZ8 2013-02-06 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS H8E at cryogenic temperature
5KYI 2016-08-03 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 9 at cryogenic temperature
1SYD 1994-07-31 1.7 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
3SK5 2011-07-06 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D at cryogenic temperature
4EQO 2012-05-02 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V99D at cryogenic temperature
4F7X 2012-06-13 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L25A at cryogenic temperature
3MVV 2011-03-16 1.72 Crystal structure of Staphylococcal nuclease variant Delta+PHS F34A at cryogenic temperature
5C3W 2015-07-15 1.72 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H at cryogenic temperature
4ZUJ 2015-05-27 1.72 Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91H at cryogenic temperature
1EY9 2000-10-18 1.72 STRUCTURE OF S. NUCLEASE STABILIZING QUADRUPLE MUTANT T41I/P117G/H124L/S128A
1II3 2003-06-17 1.72 Structure of S. nuclease quintuple mutant V23I/V66L/I72L/I92L/V99L
5J1Z 2016-04-06 1.73 Crystal structure of Staphylococcal nuclease variant Delta+PHS A90D at cryogenic temperature
4ODG 2014-01-29 1.73 Crystal structure of Staphylococcal nuclease variant V23I/V66I/V74I/V99I at cryogenic temperature
1SNM 1991-07-15 1.74 ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES
5HGT 2016-01-20 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92T at cryogenic temperature
3ITP 2010-07-21 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS F34K at cryogenic temperature
2F0S 2006-10-24 1.75 Crystal structure of Staphylococcal nuclease mutant V66L/I92V
1EY8 2000-10-18 1.75 STRUCTURE OF S. NUCLEASE STABILIZING TRIPLE MUTANT P117G/H124L/S128A
5E3F 2015-10-14 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K at cryogenic temperature
5KIX 2016-06-29 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92E at cryogenic temperature
4HTH 2012-11-28 1.75 Crystal structure of Staphylococcal nuclease variant Delta+VIAGLA at cryogenic temperature
4HMI 2012-10-31 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS V99K at cryogenic temperature
5C5L 2015-07-08 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E / L125E at cryogenic temperature
4KD3 2013-05-08 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/V66A/I92A at cryogenic temperature
1EY6 2000-10-18 1.75 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T41I
4K5V 2013-04-24 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L36A/V99T at cryogenic temperature
3P1H 2011-10-05 1.79 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/I92A at cryogenic temperature
1F2Z 2003-09-16 1.8 SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
1SYB 1994-07-31 1.8 TRANSFER OF A BETA-TURN STRUCTURE TO A NEW PROTEIN CONTEXT
3SK6 2011-07-06 1.8 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23K/L36E at cryogenic temperature
4QF4 2014-06-04 1.8 Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature
3HEJ 2010-05-19 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature
3T16 2011-08-03 1.8 Crystal structure of Staphylococcal nuclease variant NVIAGA/M98G at cryogenic temperature
5IGE 2016-03-09 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V99D at cryogenic temperature
2EYP 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant T120V
1SYE 1994-07-31 1.8 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
3BDC 2008-11-04 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS at cryogenic temperature
6OK8 2019-05-08 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS K127L at cryogenic temperature
3T13 2011-08-03 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G at cryogenic temperature
4PMB 2014-06-11 1.8 Crystal structure of Staphylcoccal nuclease variant Delta+PHS I92S at cryogenic temperature
5CV4 2015-08-12 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/V66H at cryogenic temperature
5IGC 2016-03-09 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/V66H at cryogenic temperature
3DMU 2008-11-04 1.8 Crystal structure of Staphylococcal nuclease variant PHS T62K at cryogenic temperature
5CR3 2015-08-05 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E/L125E at cryogenic temperature
4ME5 2013-10-23 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S/V66A at cryogenic temperature
2EYF 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant T44V
5IGD 2016-03-09 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperature
5C4H 2015-07-15 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92H at cryogenic temperature
5IGB 2016-03-09 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D/V66H at cryogenic temperature
3TP6 2011-09-21 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/L103K at cryogenic temperature
2EYM 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant T120C
3V2T 2011-12-28 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V66A at cryogenic temperature
5CV5 2015-08-12 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS K64G/V66K/E67G at cryogenic temperature
1KAB 1994-01-31 1.8 STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE
2F0I 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant I72L
4EQN 2012-05-02 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/I72K at cryogenic temperature
3TP5 2011-09-21 1.8 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E/L36E at cryogenic temperature
1SYF 1994-07-31 1.8 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
2F0J 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant I72V
1SYC 1994-07-31 1.8 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
2F0U 2006-10-24 1.83 Crystal structure of Staphylococcal nuclease mutant V23I/L25I/I72V
5K5P 2016-06-01 1.83 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K L103K at cryogenic temperature
1SND 1997-04-21 1.84 STAPHYLOCOCCAL NUCLEASE DIMER CONTAINING A DELETION OF RESIDUES 114-119 COMPLEXED WITH CALCIUM CHLORIDE AND THE COMPETITIVE INHIBITOR DEOXYTHYMIDINE-3',5'-DIPHOSPHATE
5C6A 2015-07-08 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E / N100E at cryogenic temperature
5EGT 2015-11-11 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E at cryogenic temperature
1TT2 2004-07-06 1.85 Cryogenic crystal structure of Staphylococcal nuclease variant truncated Delta+PHS I92K
1EZ8 2000-10-18 1.85 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V
2EY1 2006-10-24 1.85 Crystal structure of Staphylococcal nuclease mutant T22V
3SK4 2011-07-06 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N/V23E at cryogenic temperature
1STH 1995-02-27 1.85 TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES
5CGK 2015-07-22 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66D/N100E at cryogenic temperature
5IIF 2016-03-09 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D at cryogenic temperature
6AMF 2017-09-06 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36E at cryogenic temperature
1EYC 2000-10-18 1.85 STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T41I/S59A/P117G/H124L/S128A
5KYL 2016-08-03 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 7 at cryogenic temperature
5I9P 2016-03-09 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H at cryogenic temperature
3OWF 2010-10-20 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66R at cryogenic temperature
2EYJ 2006-10-24 1.85 Crystal structure of Staphylococcal nuclease mutant T62V
3ERO 2008-11-04 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72E at cryogenic temperature
6OKA 2019-05-08 1.86 Crystal structure of Staphylococcal nuclease variant Delta+PHS Q123D at cryogenic temperature
1EY7 2000-10-18 1.88 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S128A
4RKB 2014-11-26 1.88 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T/V99T at cryogenic temperature
5JAV 2016-04-27 1.89 Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91D at cryogenic temperature
1KDA 1995-02-27 1.9 STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING
5E1F 2015-10-14 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS G20E at cryogenic temperature
5KGU 2016-06-22 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS N118D at cryogenic temperature
3D4W 2009-01-06 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS A109R at cryogenic temperature
5ISR 2016-03-23 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L38E at cryogenic temperature
3NQT 2011-06-29 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A at cryogenic temperature
3R3O 2011-03-30 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature and with high redundancy
3C1E 2008-11-04 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L125K at cryogenic temperature
3S9W 2011-06-22 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature
5IOC 2016-03-23 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99D at cryogenic temperature
1SYG 1994-07-31 1.9 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
2F0G 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant V66I
3P75 2010-11-17 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104D at cryogenic temperature
5CC4 2015-07-15 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66E at cryogenic temperature
1EZ6 2000-10-18 1.9 STRUCTURE OF S. NUCLEASE STABILIZING SEXTUPLE MUTANT T33V/T41I/S59A/P117G/H124L/S128A
2EYH 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant T62S
2F0L 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant V23L/I72L
1KAA 1994-01-31 1.9 STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE
3NUC 1998-03-18 1.9 STAPHLOCOCCAL NUCLEASE, 1-N-PROPANE THIOL DISULFIDE TO V23C VARIANT
1KDB 1995-02-27 1.9 STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING
3QOL 2011-03-16 1.9 Crystal structure of Staphylococcal nuclease variant D+PHS/V23E at pH 6 determined at 100 K
1NUC 1997-06-16 1.9 STAPHYLOCOCCAL NUCLEASE, V23C VARIANT
4ZQ3 2015-05-27 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92T at cryogenic temperature
4ID6 2013-03-06 1.9 Crystal Structure of Staphylococcal nuclease mutant V23I/I72L
2F0O 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant V66I/I72V
3SK8 2011-07-06 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G apo protein at cryogenic temperature
5I6Y 2016-02-24 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS A90E at cryogenic temperature
6OK9 2019-05-08 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS K133M at cryogenic temperature
2RBM 2008-10-07 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K at cryogenic temperature
2F0K 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant V23I/L25I
3TP7 2011-09-21 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V99E at cryogenic temperature
5J22 2016-04-06 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103D at cryogenic temperature
3EJI 2008-11-04 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K at cryogenic temperature
5I6W 2016-02-24 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS A58D at cryogenic temperature
4KJO 2013-05-15 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A/V66T/V99T at cryogenic temperature
2EXZ 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant T22C
2OF1 2008-03-25 1.92 Cryogenic crystal structure of the Staphylococcal Nuclease variant truncated Delta+PHS I92W
1EQV 2003-07-08 1.94 SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
2F0E 2006-10-24 1.95 Crystal structure of Staphylococcal nuclease mutant V23L
1SNP 1997-02-12 1.95 PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE
4EVO 2012-05-23 1.95 Crystal structure of Staphylococcal nuclease variant NVIAGA/E122G at cryogenic temperature
1ENC 1994-05-31 1.95 CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING
5CV6 2015-08-12 1.95 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92E at cryogenic temperature
2F0Q 2006-10-24 1.95 Crystal structure of Staphylococcal nuclease mutant V66L/I92L
5C3X 2015-07-01 1.95 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D/N100E at cryogenic temperature
3U9O 2011-11-23 1.95 Crystal structure of Staphylococcal nuclease variant NVIAGA/N100G at cryogenic temperature
6EEG 2018-08-29 1.95 Crystal structure of Staphylcoccal nuclease variant Delta+PHS T62E at cryogenic temperature
5I9O 2016-03-23 1.95 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K L103K at cryogenic temperature
4KTA 2013-06-12 1.95 Crystal structure of Staphylococcal nuclease variant Delta+PHS R105Q at cryogenic temperatures
1A2T 1998-04-29 1.96 STAPHYLOCOCCAL NUCLEASE, B-MERCAPTOETHANOL DISULFIDE TO V23C VARIANT
2SNM 1993-01-15 1.97 IN A STAPHYLOCOCCAL NUCLEASE MUTANT THE SIDE-CHAIN of A LYSINE REPLACING VALINE 66 IS FULLY BURIED IN THE HYDROPHOBIC CORE
3D8G 2009-01-06 1.99 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72R at cryogenic temperature
3E5S 2008-11-04 2.0 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature
2OXP 2007-03-20 2.0 Crystal Structure of Staphylococcal Nuclease mutant V66D/P117G/H124L/S128A
1TQO 2004-07-06 2.0 Cryogenic Crystal Structure of Staphylococcal Nuclease Variant truncated Delta+PHS I92E
3NHH 2010-06-30 2.0 Crystal structure of Staphylococcal nuclease variant V23E-L36K at cryogenic temperature
2OEO 2008-03-25 2.0 Cryogenic crystal structure of Staphylococcal Nuclease variant truncated Delta+PHS I92D
1A2U 1998-04-29 2.0 STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 1-N-BUTANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE
4E6I 2012-04-18 2.0 Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature
1EYA 2000-10-18 2.0 STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T33V/T41I/P117G/H124L/S128A
4K8J 2013-05-01 2.0 Crystal Structure of Staphylococcal nuclease mutant V23L/V66I
1F2M 2003-09-16 2.0 SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
3D6C 2008-11-04 2.0 Crystal structure of Staphylococcal nuclease variant PHS L38E at cryogenic temperature
1F2Y 2003-09-16 2.0 SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
2NUC 1998-03-18 2.0 STAPHLOCOCCAL NUCLEASE, ETHANE THIOL DISULFIDE TO V23C VARIANT
2F0M 2006-10-24 2.0 Crystal structure of Staphylococcal nuclease mutant V23L/I72V
2EY5 2006-10-24 2.0 Crystal structure of Staphylococcal nuclease mutant T41S
1KDC 1995-02-27 2.0 STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING
3C1F 2008-11-04 2.0 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104K at cryogenic temperature
1STB 1994-07-31 2.0 ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE
3EVQ 2008-11-04 2.0 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E at cryogenic temperature
2F0V 2006-10-24 2.0 Crystal structure of Staphylococcal nuclease mutant V23L/V66L/I72L
3HZX 2010-05-19 2.0 Crystal Structure of Staphylococcal nuclease variant D+PHS/V66K at pH 9 determined at 100 K
2RKS 2008-04-15 2.01 Crystal structure of Staphylococcal nuclease variant PHS L38K at cryogenic temperature
2RDF 2008-06-10 2.01 Crystal Structure of staphyloccocal nuclease VIAGAN/E75A variant at cryogenic temperature
2F0P 2006-10-24 2.05 Crystal structure of Staphylococcal nuclease mutant V66I/V99I
2ENB 1994-05-31 2.05 CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING
1A3U 1998-04-29 2.05 STAPHYLOCOCCAL NUCLEASE, CYCLOHEXANE THIOL DISULFIDE TO V23C VARIANT
3D4D 2008-11-04 2.1 Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature
4K8I 2013-05-01 2.1 Crystal Structure of Staphylococcal Nuclease mutant I92V/V99L
1AEX 1997-06-16 2.1 STAPHYLOCOCCAL NUCLEASE, METHANE THIOL DISULFIDE TO V23C VARIANT
1U9R 2004-12-21 2.1 Crystal Structure of Staphylococcal Nuclease mutant V66E/P117G/H124L/S128A at Room Temperature
2F0W 2006-10-24 2.1 Crystal structure of Staphylococcal nuclease mutant V23I/L25I/V66L/I72L
5EKL 2015-11-18 2.1 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D/N100E at cryogenic temperature
1TR5 2004-07-06 2.1 Room temperature structure of Staphylococcal nuclease variant truncated Delta+PHS I92E
1A3T 1998-04-29 2.1 STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 2-FLUOROETHANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE
5NUC 1998-04-29 2.1 STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT
3ERQ 2008-11-04 2.1 Crystal structure of Staphylococcal nuclease variant L25K at cryogenic temperature
5EKK 2015-11-18 2.1 Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D/L125E at cryogenic temperature
2F0F 2006-10-24 2.15 Crystal structure of Staphylococcal nuclease mutant L25I
1ENA 1994-05-31 2.15 CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING
3DHQ 2009-01-06 2.15 Crystal structure of Staphylococcal nuclease variant Delta+PHS A90R at cryogenic temperature
2F0H 2006-10-24 2.15 Crystal structure of Staphylococcal nuclease mutant V66L
6EEK 2018-08-29 2.2 Crystal structure of apo Staphylcoccal nuclease variant Delta+PHS T62E/V66K, pH 7 at cryogenic temperature
2QDB 2008-07-01 2.2 Crystal structure of staphylococcal nuclease variant E75Q/D21N/T33V/T41I/S59A/P117G/S128A at 100 K
1A3V 1998-04-29 2.2 STAPHYLOCOCCAL NUCLEASE, CYCLOPENTANE THIOL DISULFIDE TO V23C VARIANT
2F0D 2006-10-24 2.5 Crystal structure of Staphylococcal nuclease mutant I92V
1NSN 1995-09-15 2.8 THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION
2GSI 2006-05-16 2.81 Crystal Structure of a Murine Fab in Complex with an 11 Residue Peptide Derived from Staphylococcal Nuclease

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.3 Thermonuclease Q6GIK1 NUC_STAAR
99.3 Thermonuclease Q8NXI6 NUC_STAAW
99.3 Thermonuclease Q6GB41 NUC_STAAS
99.1 Thermonuclease Q7A6P2 NUC_STAAN
99.1 Thermonuclease Q99VJ0 NUC_STAAM
99.3 Thermonuclease Q5HHM4 NUC_STAAC
100.0 Thermonuclease P00644 NUC_STAAU