Folding and stability of a fibronectin type III domain of human tenascin.


Abstract

The folding of an isolated fibronectin type III domain of human tenascin, a large extra-cellular matrix protein, has been characterised. The isolated module, which has no disulphide bonds, can be reversibly unfolded by chemical denaturant and temperature. Equilibrium unfolding, measured using a number of different probes, fits to a two-state transition, with consistent measures of DeltaGH2OD-N. Folding and refolding rate constants have been determined over a range of denaturant concentrations. The refolding kinetics are bi-phasic, and in the transition region the slow phase dominates refolding kinetics. Outside the transition region the folding of the fast-folding species fits to a two-state model. There is no evidence for significant accumulation of partially folded intermediates. Study holds ProTherm entries: 8887, 8888, 8889, 8890, 8891, 8892, 8893, 8894, 8895, 8896 Extra Details: Fluorescence measurements at 350nm tenascin; fibronectin type III; immunoglobulin; protein folding; stability

Submission Details

ID: xfkYQ7rA4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:37 p.m.

Version: 1

Publication Details
Clarke J;Hamill SJ;Johnson CM,J. Mol. Biol. (1997) Folding and stability of a fibronectin type III domain of human tenascin. PMID:9245604
Additional Information

Study Summary

Number of data points 22
Proteins Fibronectin ; Fibronectin
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: Tm pH:7.0, buffers:Sodium phosphate: 50 mM, prot_conc:4 micro M ; Experimental Assay: Cm pH:7.0, buffers:Sodium phosphate: 50 mM, prot_conc:4 micro M ; Experimental Assay: m pH:7.0, buffers:Sodium phosphate: 50 mM, prot_conc:4 micro M ; Experimental Assay: dG_H2O pH:7.0, buffers:Sodium phosphate: 50 mM, prot_conc:4 micro M ; Experimental Assay: Cm prot_conc:1 micro M, buffers:Sodium phosphate: 50 mM, pH:7.0 ; Experimental Assay: m prot_conc:1 micro M, buffers:Sodium phosphate: 50 mM, pH:7.0 ; Experimental Assay: dG_H2O prot_conc:1 micro M, buffers:Sodium phosphate: 50 mM, pH:7.0 ; Experimental Assay: Cm pH:7.0, buffers:Sodium phosphate: 50 mM, prot_conc:25 micro M ; Experimental Assay: m pH:7.0, buffers:Sodium phosphate: 50 mM, prot_conc:25 micro M ; Experimental Assay: dG_H2O pH:7.0, buffers:Sodium phosphate: 50 mM, prot_conc:25 micro M ; Experimental Assay: Tm pH:5.0, prot_conc:4 micro MM, buffers:Sodium acetate: 50 mM ; Experimental Assay: Tm prot_conc:1 micro M, pH:5.0, buffers:Sodium acetate: 50 mM ; Experimental Assay: Cm prot_conc:0.12 mg/ml, pH:5.0, buffers:Sodium acetate: 50 mM ; Experimental Assay: m prot_conc:0.12 mg/ml, pH:5.0, buffers:Sodium acetate: 50 mM ; Experimental Assay: dG_H2O prot_conc:0.12 mg/ml, pH:5.0, buffers:Sodium acetate: 50 mM
Libraries Mutations for sequence VSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRT

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1E88 2000-09-18T00:00:00+0000 0 Solution structure of 6F11F22F2, a compact three-module fragment of the gelatin-binding domain of human fibronectin
1E8B 2000-09-18T00:00:00+0000 0 Solution structure of 6F11F22F2, a compact three-module fragment of the gelatin-binding domain of human fibronectin
1FBR 1995-08-08T00:00:00+0000 0 FOURTH AND FIFTH FIBRONECTIN TYPE I MODULE PAIR
1FNA 1994-01-11T00:00:00+0000 1.8 CRYSTAL STRUCTURE OF THE TENTH TYPE III CELL ADHESION MODULE OF HUMAN FIBRONECTIN
1FNF 1995-09-30T00:00:00+0000 2.0 FRAGMENT OF HUMAN FIBRONECTIN ENCOMPASSING TYPE-III REPEATS 7 THROUGH 10
1FNH 1999-01-28T00:00:00+0000 2.8 CRYSTAL STRUCTURE OF HEPARIN AND INTEGRIN BINDING SEGMENT OF HUMAN FIBRONECTIN
1J8K 2001-05-22T00:00:00+0000 0 NMR STRUCTURE OF THE FIBRONECTIN EDA DOMAIN, NMR, 20 STRUCTURES
1O9A 2002-12-11T00:00:00+0000 0 Solution structure of the complex of 1F12F1 from fibronectin with B3 from FnBB from S. dysgalactiae
1OWW 2003-03-31T00:00:00+0000 0 Solution structure of the first type III module of human fibronectin determined by 1H, 15N NMR spectroscopy
1Q38 2003-07-28T00:00:00+0000 0 Anastellin

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Fibronectin P02751 FINC_HUMAN
96.8 Fibronectin P07589 FINC_BOVIN
94.0 Fibronectin Q28275 FINC_CANLF
92.1 Fibronectin Q28377 FINC_HORSE
90.9 Fibronectin Q91400 FINC_NOTVI