Epitope mapping by mutagenesis distinguishes between the two tertiary structures of the histidine-containing protein HPr.


Abstract

Thirty-four of the 85 residues of the histidine-containing protein HPr of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system have been changed by site-directed mutagenesis. Many of the mutations have wild-type activity suggesting an unaltered tertiary structure but have altered binding to three monoclonal antibodies: Jel42, Jel44, and Jel323. This altered binding defines the residues that are involved in the epitopes of HPr. At present, two different three-dimensional structures have been determined for HPr, one from two-dimensional nuclear magnetic resonance spectra and the other from x-ray diffraction of HPr crystals. The epitope mapping for Jel42 does not distinguish between the tertiary structures. However, only the HPr structure derived from two-dimensional nuclear magnetic resonance spectra is consistent with a contiguous surface binding site that can be defined as the epitope for Jel44. Thus the x-ray structure may represent a partially unfolded HPr.

Submission Details

ID: xawcnBDF

Submitter: Shu-Ching Ou

Submission Date: Feb. 27, 2019, 12:36 p.m.

Version: 1

Publication Details
Sharma S;Georges F;Delbaere LT;Lee JS;Klevit RE;Waygood EB,Proc Natl Acad Sci U S A (1991) Epitope mapping by mutagenesis distinguishes between the two tertiary structures of the histidine-containing protein HPr. PMID:1711212
Additional Information

Sequences for Jel44 and Jel323 are not found, thus the sequences/mutants associated with the binding data do not include sequences of these two monoclonal antibodies.

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Phosphocarrier protein HPr P0AA06 PTHP_ECO57
100.0 Phosphocarrier protein HPr P0AA05 PTHP_ECOL6
100.0 Phosphocarrier protein HPr P0AA04 PTHP_ECOLI
100.0 Phosphocarrier protein HPr P0AA08 PTHP_SALTI
100.0 Phosphocarrier protein HPr P0AA07 PTHP_SALTY
100.0 Phosphocarrier protein HPr P0AA09 PTHP_SHIFL
98.8 Phosphocarrier protein HPr P16481 PTHP_KLEPN