DSC studies of Fusarium solani pisi cutinase: consequences for stability in the presence of surfactants.


Abstract

The application of cutinase from Fusarium solani pisi as a fat-stain removing ingredient in laundry washing is hampered by its lack of stability in the presence of anionic surfactants. We postulate that the stability of cutinase towards anionics can be improved by mutations increasing its temperature stability. Thermal unfolding as measured with DSC, appears to be irreversible, though the thermograms are more symmetric than predicted by a simple irreversible model. In the presence of taurodeoxycholate (TDOC), the unfolding temperature is lower and the unfolding is reversible. We conclude that an early reversible unfolding intermediate exists in which a number of additional hydrophobic patches are exposed to the solvent, or preferentially are covered with TDOC. Improvement of the stability of cutinase with respect to both surfactants and thermal denaturation, should thus be directed toward the prevention of exposure of hydrophobic patches in the early intermediate. Study holds ProTherm entries: 11754, 11755, 11756, 11757 Extra Details: Scan rate 1.0 K/min cutinase; lipase; protein stabirity; protein unfolding; surfactants;,differential scanning calorimetry(DSC)

Submission Details

ID: xQWPm9Ji3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:43 p.m.

Version: 1

Publication Details
Creveld LD;Meijberg W;Berendsen HJ;Pepermans HA,Biophys. Chem. (2001) DSC studies of Fusarium solani pisi cutinase: consequences for stability in the presence of surfactants. PMID:11527580
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1AGY 1997-03-26T00:00:00+0000 1.15 The 1.15 angstrom refined structure of fusarium solani pisi cutinase
1CEX 1997-02-18T00:00:00+0000 1.0 STRUCTURE OF CUTINASE
1CUA 1995-11-16T00:00:00+0000 1.8 CUTINASE, N172K MUTANT
1CUB 1995-11-16T00:00:00+0000 1.75 CUTINASE, N172K, R196D MUTANT, MONOCLINIC CRYSTAL FORM
1CUC 1995-11-16T00:00:00+0000 1.75 CUTINASE, N172K, R196D MUTANT, ORTHORHOMBIC CRYSTAL FORM
1CUD 1995-11-16T00:00:00+0000 2.7 CUTINASE, N172K, R196D MUTANT, MONOCLINIC CRYSTAL FORM WITH THREE MOLECULES PER ASYMMETRIC UNIT
1CUE 1995-11-16T00:00:00+0000 2.1 CUTINASE, Q121L MUTANT
1CUF 1995-11-16T00:00:00+0000 1.75 CUTINASE, R156L MUTANT
1CUG 1995-11-16T00:00:00+0000 1.75 CUTINASE, R17E, N172K MUTANT
1CUH 1995-11-16T00:00:00+0000 1.75 CUTINASE, R196E MUTANT

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Cutinase 1 P00590 CUTI1_FUSSO
99.0 Cutinase 1 Q99174 CUTI_FUSSC
94.0 Cutinase 1 Q96UT0 CUTI2_FUSSO
94.0 Cutinase 1 Q96US9 CUTI3_FUSSO