DSC studies of Fusarium solani pisi cutinase: consequences for stability in the presence of surfactants.


Abstract

The application of cutinase from Fusarium solani pisi as a fat-stain removing ingredient in laundry washing is hampered by its lack of stability in the presence of anionic surfactants. We postulate that the stability of cutinase towards anionics can be improved by mutations increasing its temperature stability. Thermal unfolding as measured with DSC, appears to be irreversible, though the thermograms are more symmetric than predicted by a simple irreversible model. In the presence of taurodeoxycholate (TDOC), the unfolding temperature is lower and the unfolding is reversible. We conclude that an early reversible unfolding intermediate exists in which a number of additional hydrophobic patches are exposed to the solvent, or preferentially are covered with TDOC. Improvement of the stability of cutinase with respect to both surfactants and thermal denaturation, should thus be directed toward the prevention of exposure of hydrophobic patches in the early intermediate. Study holds ProTherm entries: 11754, 11755, 11756, 11757 Extra Details: Scan rate 1.0 K/min cutinase; lipase; protein stabirity; protein unfolding; surfactants;,differential scanning calorimetry(DSC)

Submission Details

ID: xQWPm9Ji3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:43 p.m.

Version: 1

Publication Details
Creveld LD;Meijberg W;Berendsen HJ;Pepermans HA,Biophys. Chem. (2001) DSC studies of Fusarium solani pisi cutinase: consequences for stability in the presence of surfactants. PMID:11527580
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3QPA 2012-02-29 0.85 Structure of Fusarium Solani Cutinase expressed in Pichia pastoris
3QPC 2012-02-29 0.98 Structure of Fusarium Solani Cutinase expressed in Pichia pastoris, crystallized in the presence of Paraoxon
1CEX 1997-08-20 1.0 STRUCTURE OF CUTINASE
1AGY 1998-04-01 1.15 The 1.15 angstrom refined structure of fusarium solani pisi cutinase
3ESC 2009-07-28 1.2 cut-2a; NCN-Pt-Pincer-Cutinase Hybrid
3ESD 2009-07-28 1.22 cut-2b; NCN-Pt-Pincer-Cutinase Hybrid
1CUS 1994-07-31 1.25 FUSARIUM SOLANI CUTINASE IS A LIPOLYTIC ENZYME WITH A CATALYTIC SERINE ACCESSIBLE TO SOLVENT
3EF3 2009-07-28 1.5 cut-1a; NCN-Pt-Pincer-Cutinase Hybrid
1CUJ 1996-07-11 1.6 CUTINASE, S120C MUTANT
1FFA 1996-03-08 1.69 CONTRIBUTION OF CUTINASE SERINE 42 SIDE CHAIN TO THE STABILIZATION OF THE OXYANION TRANSITION STATE
1CUU 1996-07-11 1.69 CUTINASE, A199C MUTANT
1XZF 1996-10-14 1.69 FUSARIUM SOLANI CUTINASE MUTANT WITH THR 144 REPLACED BY CYS
1FFD 1996-03-08 1.69 CONTRIBUTION OF CUTINASE SERINE 42 SIDE CHAIN TO THE STABILIZATION OF THE OXYANION TRANSITION STATE
1XZG 1996-10-14 1.69 FUSARIUM SOLANI CUTINASE MUTANT WITH THR 45 REPLACED BY ALA
1CUY 1996-07-11 1.69 CUTINASE, L189F MUTANT
1FFE 1996-03-08 1.69 CONTRIBUTION OF CUTINASE SERINE 42 SIDE CHAIN TO THE STABILIZATION OF THE OXYANION TRANSITION STATE
1XZI 1996-10-14 1.69 FUSARIUM SOLANI CUTINASE MUTANT WITH THR 119 REPLACED BY HIS
1XZJ 1996-10-14 1.69 FUSARIUM SOLANI CUTINASE MUTANT WITH THR 38 REPLACED BY PHE
1XZH 1996-10-14 1.69 FUSARIUM SOLANI CUTINASE MUTANT WITH THR 80 REPLACED BY PRO
1XZL 1996-11-30 1.69 FUSARIUM SOLANI CUTINASE COMPLEX WITH N-HEXYLPHOSPHONATE ETHYL ESTER
1XZC 1996-10-14 1.75 FUSARIUM SOLANI CUTINASE MUTANT WITH SER 129 REPLACED BY CYS COMPLEX WITH PARA-SULFUROUSPHENYL MERCURY
1CUB 1996-07-11 1.75 CUTINASE, N172K, R196D MUTANT, MONOCLINIC CRYSTAL FORM
1CUX 1996-07-11 1.75 CUTINASE, L114Y MUTANT
1XZB 1996-10-14 1.75 FUSARIUM SOLANI CUTINASE MUTANT WITH SER 129 REPLACED BY CYS COMPLEX WITH MERCURY ACETATE
1FFC 1996-03-08 1.75 CONTRIBUTION OF CUTINASE SERINE 42 SIDE CHAIN TO THE STABILIZATION OF THE OXYANION TRANSITION STATE
1CUG 1996-07-11 1.75 CUTINASE, R17E, N172K MUTANT
1XZE 1996-10-14 1.75 FUSARIUM SOLANI CUTINASE MUTANT WITH SER 92 REPLACED BY CYS
1FFB 1996-03-08 1.75 CONTRIBUTION OF CUTINASE SERINE 42 SIDE CHAIN TO THE STABILIZATION OF THE OXYANION TRANSITION STATE
1XZM 1996-11-30 1.75 FUSARIUM SOLANI CUTINASE COMPLEX WITH N-UNDECYL O-METHYL CHLORO PHOSPHONATE ESTER
1CUH 1996-07-11 1.75 CUTINASE, R196E MUTANT
1CUC 1996-07-11 1.75 CUTINASE, N172K, R196D MUTANT, ORTHORHOMBIC CRYSTAL FORM
1CUF 1996-07-11 1.75 CUTINASE, R156L MUTANT
1CUA 1996-07-11 1.8 CUTINASE, N172K MUTANT
1XZA 1996-10-14 1.8 FUSARIUM SOLANI CUTINASE MUTANT WITH SER 129 REPLACED BY CYS
2CUT 1994-08-31 1.9 CUTINASE, A LIPOLYTIC ENZYME WITH A PREFORMED OXYANION HOLE
3ESA 2009-07-28 2.0 cut-1b; NCN-Pt-Pincer-Cutinase Hybrid
1CUV 1996-07-11 2.01 CUTINASE, A85F MUTANT
1XZK 1996-11-30 2.01 FUSARIUM SOLANI CUTINASE COMPLEX WITH DI(ISOPROPYL)PHOSPHATE
1CUZ 1996-07-11 2.1 CUTINASE, L81G, L182G MUTANT
1CUE 1996-07-11 2.1 CUTINASE, Q121L MUTANT
1OXM 1997-05-15 2.3 STRUCTURE OF CUTINASE
3ESB 2009-07-28 2.3 cut-1c; NCN-Pt-Pincer-Cutinase Hybrid
1CUI 1996-07-11 2.5 CUTINASE, S120A MUTANT
1XZD 1996-10-14 2.7 FUSARIUM SOLANI CUTINASE MUTANT WITH SER 213 REPLACED BY CYS
1CUW 1996-07-11 2.7 CUTINASE, G82A, A85F, V184I, A185L, L189F MUTANT
1CUD 1996-07-11 2.7 CUTINASE, N172K, R196D MUTANT, MONOCLINIC CRYSTAL FORM WITH THREE MOLECULES PER ASYMMETRIC UNIT

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
94.0 Cutinase 1 Q96US9 CUTI3_FUSSO
94.0 Cutinase 1 Q96UT0 CUTI2_FUSSO
99.0 Cutinase 1 Q99174 CUTI_FUSSC
100.0 Cutinase 1 P00590 CUTI1_FUSSO