Stabilization of hen egg white lysozyme by a cavity-filling mutation.


Stabilization of a protein using cavity-filling strategy has hardly been successful because of unfavorable van der Waals contacts. We succeeded in stabilizing lysozymes by cavity-filling mutations. The mutations were checked by a simple energy minimization in advance. It was shown clearly that the sum of free energy change caused by the hydrophobicity and the cavity size was correlated very well with protein stability. We also considered the aromatic-aromatic interaction. It is reconfirmed that the cavity-filling mutation in a hydrophobic core is a very useful method to stabilize a protein when the mutation candidate is selected carefully. Study holds ProTherm entries: 12725, 12726, 12727, 12728, 12729, 12730, 14301, 14302, 14303, 14304, 14305 Extra Details: In the presence of 0.7 M GdnHCl. beta-trefoil; fibroblast growth factor; core-packing; protein engineering;,protein evolution

Submission Details

ID: xNVv77tw3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:44 p.m.

Version: 1

Publication Details
Ohmura T;Ueda T;Ootsuka K;Saito M;Imoto T,Protein Sci. (2001) Stabilization of hen egg white lysozyme by a cavity-filling mutation. PMID:11266617
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Fibroblast growth factor 1 P05230 FGF1_HUMAN
100.0 Fibroblast growth factor 1 Q5NVQ3 FGF1_PONAB
97.9 Fibroblast growth factor 1 P34004 FGF1_MESAU
98.0 Fibroblast growth factor 1 P20002 FGF1_PIG
96.4 Fibroblast growth factor 1 P61148 FGF1_MOUSE
96.4 Fibroblast growth factor 1 P61149 FGF1_RAT
92.3 Fibroblast growth factor 1 P03968 FGF1_BOVIN
90.7 Fibroblast growth factor 1 Q7M303 FGF1_SHEEP
90.3 Fibroblast growth factor 1 P19596 FGF1_CHICK
91.5 Fibroblast growth factor 1 Q9N1S8 FGF1_CAPCA