Two-state irreversible thermal denaturation of muscle creatine kinase.
Abstract
Thermal denaturation of creatine kinase from rabbit skeletal muscle has been studied by differential scanning calorimetry. The excess heat capacity vs. temperature profiles were independent of protein concentration, but strongly temperature scanning rate-dependent. It has been shown that thermal denaturation of creatine kinase satisfies the previously proposed validity criteria for the two-state irreversible model [Kurganov et al., Biophys. Chem.70 (1997) 125]. The energy activation value has been calculated to be 461.0 +/- 0.7 kJ/mol. Study holds ProTherm entries: 9198 Extra Details: creatine kinase; irreversible thermal denaturation;,differential scanning calorimetry
Submission Details
ID: wmW7X3hP4
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:37 p.m.
Version: 1
Publication Details
Lyubarev AE;Kurganov BI;Orlov VN;Zhou HM,Biophys. Chem. (1999) Two-state irreversible thermal denaturation of muscle creatine kinase. PMID:10443013Additional Information
Study Summary
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
|---|
Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant UniProtKB Entries
| Percent Identity | Matching Chains | Protein | Accession | Entry Name |
|---|---|---|---|---|
| 100.0 | Creatine kinase B-type | P00567 | KCRB_RABIT | |
| 97.4 | Creatine kinase B-type | P05124 | KCRB_CANLF | |
| 96.9 | Creatine kinase B-type | Q5EA61 | KCRB_BOVIN | |
| 96.9 | Creatine kinase B-type | Q29594 | KCRB_PIG | |
| 96.6 | Creatine kinase B-type | P12277 | KCRB_HUMAN | |
| 95.3 | Creatine kinase B-type | Q04447 | KCRB_MOUSE | |
| 95.3 | Creatine kinase B-type | P07335 | KCRB_RAT | |
| 99.2 | Creatine kinase M-type | P00563 | KCRM_RABIT | |
| 97.1 | Creatine kinase M-type | P07310 | KCRM_MOUSE | |
| 97.1 | Creatine kinase M-type | P00564 | KCRM_RAT | |
| 96.6 | Creatine kinase M-type | Q5XLD3 | KCRM_PIG | |
| 96.6 | Creatine kinase M-type | Q9XSC6 | KCRM_BOVIN | |
| 96.1 | Creatine kinase M-type | P05123 | KCRM_CANLF | |
| 95.8 | Creatine kinase M-type | P06732 | KCRM_HUMAN | |
| 90.6 | Creatine kinase M-type | P00565 | KCRM_CHICK |