Two-state irreversible thermal denaturation of muscle creatine kinase.


Abstract

Thermal denaturation of creatine kinase from rabbit skeletal muscle has been studied by differential scanning calorimetry. The excess heat capacity vs. temperature profiles were independent of protein concentration, but strongly temperature scanning rate-dependent. It has been shown that thermal denaturation of creatine kinase satisfies the previously proposed validity criteria for the two-state irreversible model [Kurganov et al., Biophys. Chem.70 (1997) 125]. The energy activation value has been calculated to be 461.0 +/- 0.7 kJ/mol. Study holds ProTherm entries: 9198 Extra Details: creatine kinase; irreversible thermal denaturation;,differential scanning calorimetry

Submission Details

ID: wmW7X3hP4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:37 p.m.

Version: 1

Publication Details
Lyubarev AE;Kurganov BI;Orlov VN;Zhou HM,Biophys. Chem. (1999) Two-state irreversible thermal denaturation of muscle creatine kinase. PMID:10443013
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1U6R 2005-08-02 1.65 Transition state analog complex of muscle creatine kinase (R134K) mutant
4Q2R 2014-09-03 1.65 Structural Proteomics From Crude Native Rod Outer Segments
3DRB 2009-03-17 2.0 Crystal structure of Human Brain-type Creatine Kinase
3B6R 2008-11-04 2.0 Crystal structure of Human Brain-type Creatine Kinase
3DRE 2009-03-17 2.2 Crystal structure of Human Brain-type Creatine Kinase
1G0W 2001-02-07 2.3 CRYSTAL STRUCTURE OF BOVINE RETINAL CREATINE KINASE
2CRK 1999-01-13 2.35 MUSCLE CREATINE KINASE
1I0E 2003-04-01 3.5 CRYSTAL STRUCTURE OF CREATINE KINASE FROM HUMAN MUSCLE

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
95.3 Creatine kinase B-type P07335 KCRB_RAT
95.3 Creatine kinase B-type Q04447 KCRB_MOUSE
96.6 Creatine kinase B-type P12277 KCRB_HUMAN
96.9 Creatine kinase B-type Q29594 KCRB_PIG
96.9 Creatine kinase B-type Q5EA61 KCRB_BOVIN
97.4 Creatine kinase B-type P05124 KCRB_CANLF
100.0 Creatine kinase B-type P00567 KCRB_RABIT
90.6 Creatine kinase M-type P00565 KCRM_CHICK
95.8 Creatine kinase M-type P06732 KCRM_HUMAN
96.1 Creatine kinase M-type P05123 KCRM_CANLF
96.6 Creatine kinase M-type Q9XSC6 KCRM_BOVIN
96.6 Creatine kinase M-type Q5XLD3 KCRM_PIG
97.1 Creatine kinase M-type P00564 KCRM_RAT
97.1 Creatine kinase M-type P07310 KCRM_MOUSE
99.2 Creatine kinase M-type P00563 KCRM_RABIT