Thermal stability and DNA binding activity of a variant form of the Sso7d protein from the archeon Sulfolobus solfataricus truncated at leucine 54.


Abstract

Sso7d is a 62-residue, basic protein from the hyperthermophilic archaeon Sulfolobus solfataricus. Around neutral pH, it exhibits a denaturation temperature close to 100 degrees C and a non-sequence-specific DNA binding activity. Here, we report the characterization by circular dichroism and fluorescence measurements of a variant form of Sso7d truncated at leucine 54 (L54Delta). It is shown that L54Delta has a folded conformation at neutral pH and that its thermal unfolding is a reversible process, represented well by the two-state N <=> D transition model, with a denaturation temperature of 53 degrees C. Fluorescence titration experiments indicate that L54Delta binds tightly to calf thymus DNA, even though the binding parameters are smaller than those of the wild-type protein. Therefore, the truncation of eight residues at the C-terminus of Sso7d markedly affects the thermal stability of the protein, which nevertheless retains a folded structure and DNA binding activity. Study holds ProTherm entries: 16475, 16476, 16477, 16478, 16479, 16480, 16481 Extra Details: hyperthermophilic archaeon; non-sequence-specific; DNA binding activity; thermal stability

Submission Details

ID: vgp7BKRy3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:48 p.m.

Version: 1

Publication Details
Shehi E;Granata V;Del Vecchio P;Barone G;Fusi P;Tortora P;Graziano G,Biochemistry (2003) Thermal stability and DNA binding activity of a variant form of the Sso7d protein from the archeon Sulfolobus solfataricus truncated at leucine 54. PMID:12846585
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1BBX 1998-10-14 NON-SPECIFIC PROTEIN-DNA INTERACTIONS IN THE SSO7D-DNA COMPLEX, NMR, 1 STRUCTURE
1B4O 2000-01-05 NMR STUDY OF SSO7D MUTANT (F31A) MINIMIZED AVERAGE STRUCTURE
1JIC 1998-10-14 SOLUTION NMR STRUCTURE OF RECOMBINANT SSO7D WITH RNASE ACTIVITY, MINIMIZED AVERAGE STRUCTURE
1SSO 1995-05-08 SOLUTION STRUCTURE AND DNA-BINDING PROPERTIES OF A THERMOSTABLE PROTEIN FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS
2CVR 2006-08-29 NMR solution structure of sso7d mutant, K12L, 12 conformers
1C8C 2001-05-04 1.45 CRYSTAL STRUCTURES OF THE CHROMOSOMAL PROTEINS SSO7D/SAC7D BOUND TO DNA CONTAINING T-G MISMATCHED BASE PAIRS
1BNZ 1998-11-11 2.0 SSO7D HYPERTHERMOPHILE PROTEIN/DNA COMPLEX
5B02 2016-03-23 2.21 Structure of the prenyltransferase MoeN5 with a fusion protein tag of Sso7d
6J8V 2019-04-17 2.23 Structure of MOEN5-SSO7D fusion protein in complex with ligand 2
5B0I 2016-03-23 2.26 Structure of MoeN5-Sso7d fusion protein in complex with beta-octyl glucoside
5GWW 2017-09-20 2.3 Structure of MoeN5-Sso7d fusion protein in complex with a permethylated substrate analogue
6J8W 2019-04-17 2.35 Structure of MOEN5-SSO7D fusion protein in complex with lig 1
5GWV 2017-09-20 2.4 Structure of MoeN5-Sso7d fusion protein in complex with a substrate analogue
5B0J 2016-03-23 2.5 Structure of MoeN5-Sso7d fusion protein in complex with beta-undecyl maltoside
5B03 2016-03-23 2.6 Structure of MoeN5-Sso7d fusion protein in complex with geranyl pyrophosphate
5B0K 2016-03-23 2.75 Structure of MoeN5-Sso7d fusion protein in complex with beta-decyl maltoside
5B0L 2016-03-23 2.8 Structure of MoeN5-Sso7d fusion protein in complex with beta-nonyl glucoside
5B0M 2016-03-23 3.05 Structure of MoeN5-Sso7d fusion protein in complex with beta-dodecyl maltoside
5U1C 2017-01-11 3.9 Structure of tetrameric HIV-1 Strand Transfer Complex Intasome

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
93.2 DNA-binding protein 7a Q96X56 DN7_SULTO
98.4 DNA-binding protein 7a F0NJT3 DN7B_SULIH
96.9 DNA-binding protein 7d O59632 DN7B_SACSH
100.0 DNA-binding protein 7a D2PHL8 DN7A_SULID
100.0 DNA-binding protein 7a P61990 DN7A_SACSH
100.0 DNA-binding protein 7a P61991 DN7A_SACS2
100.0 DNA-binding protein 7d P39476 DN7D_SACS2