Domain interactions and connecting peptides in lens crystallins.


Abstract

beta B2- and gamma B-crystallin from bovine eye-lens are closely related proteins, topologically distinct mainly by virtue of the linker peptide connecting the two domains in each polypeptide chain. In homodimeric beta B2-crystallin, the extended conformation of the connecting peptide has been suggested to force the beta B2-molecule to favor intermolecular domain interactions compared with intramolecular contacts in monomeric gamma B-crystallin. From this one may postulate that the conserved interdomain contacts are essential for the overall stability of crystallins. This was clearly confirmed for gamma B-crystallin, since its isolated C-terminal domain is significantly less stable than in the context of native gamma B. Exchanging the linker peptide of gamma B- for that of beta B2-crystallin yields a monomeric protein with stability characteristics identical to gamma B-crystallin. We conclude that the domain-interface itself rather than the connecting peptide determines the mode of domain association in crystallins, as the linker in the gamma B beta-mutant is evidently twisted to a turn similar to the one in natural gamma B-crystallin. Study holds ProTherm entries: 10022 Extra Details: additive : EDTA(1 mM), crystallins; protein folding; protein stability; domain interactions; connecting peptide

Submission Details

ID: vZQzrJDM4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:39 p.m.

Version: 1

Publication Details
Mayr EM;Jaenicke R;Glockshuber R,J. Mol. Biol. (1994) Domain interactions and connecting peptides in lens crystallins. PMID:8289268
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3L1E 2010-05-12 1.15 Bovine AlphaA crystallin Zinc Bound
3L1F 2010-05-12 1.53 Bovine AlphaA crystallin

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
90.8 Alpha-crystallin A chain P02487 CRYAA_BRAVA
90.2 Alpha-crystallin A chain P02502 CRYAA_MACRU
91.9 Alpha-crystallin A chain P02486 CRYAA_CHOHO
90.8 Alpha-crystallin A chain P02501 CRYAA_ORYAF
92.5 Alpha-crystallin A chain P82533 CRYAA_ERIEU
92.5 Alpha-crystallin A chain P02499 CRYAA_PROCA
94.8 Alpha-crystallin A chain P02488 CRYAA_MACMU
94.2 Alpha-crystallin A chain P02498 CRYAA_LOXAF
94.2 Alpha-crystallin A chain P02489 CRYAA_HUMAN
94.2 Alpha-crystallin A chain A0A140G945 CRYA2_HUMAN
95.4 Alpha-crystallin A chain P02484 CRYAA_MANJA
96.0 Alpha-crystallin A chain P68406 CRYAA_TUPGL
96.0 Alpha-crystallin A chain P68405 CRYAA_MERUN
95.4 Alpha-crystallin A chain P68286 CRYAA_PERPO
95.4 Alpha-crystallin A chain P68287 CRYAA_OTOCR
96.0 Alpha-crystallin A chain P02492 CRYAA_OCHPR
96.0 Alpha-crystallin A chain P02483 CRYAA_NEOVI
96.5 Alpha-crystallin A chain P68288 CRYAA_ZALCA
96.5 Alpha-crystallin A chain P68289 CRYAA_HALGR
96.0 Alpha-crystallin A chain P02494 CRYAA_EULFU
97.1 Alpha-crystallin A chain P02482 CRYAA_ARTJA
96.5 Alpha-crystallin A chain P68283 CRYAA_PEDCA
96.5 Alpha-crystallin A chain P68281 CRYAA_CAVPO
97.1 Alpha-crystallin A chain P02477 CRYAA_PHOPH
97.1 Alpha-crystallin A chain P02480 CRYAA_MELUS
97.1 Alpha-crystallin A chain P02478 CRYAA_HORSE
97.1 Alpha-crystallin A chain P02493 CRYAA_RABIT
98.3 Alpha-crystallin A chain P68282 CRYAA_FELCA
98.3 Alpha-crystallin A chain P68280 CRYAA_CANLF
98.8 Alpha-crystallin A chain P02472 CRYAA_CAMDR
97.7 Alpha-crystallin A chain P02479 CRYAA_CERSI
97.7 Alpha-crystallin A chain P02475 CRYAA_PIG
98.3 Alpha-crystallin A chain P02474 CRYAA_BALAC
98.3 Alpha-crystallin A chain P02476 CRYAA_TAPIN
98.8 Alpha-crystallin A chain P82531 CRYAA_PTEPO
99.4 Alpha-crystallin A chain P68285 CRYAA_HIPAM
99.4 Alpha-crystallin A chain P68284 CRYAA_GIRCA
99.4 Alpha-crystallin A chain Q5ENZ0 CRYAA_SHEEP
100.0 Alpha-crystallin A chain P02470 CRYAA_BOVIN