Thermodynamics of BPTI folding.


Abstract

A calorimetric study of the basic pancreatic trypsin inhibitor (BPTI) has been performed using the new generation of the adiabatic scanning microcalorimeters, operating in an extended temperature range of 5-130 degrees C. Precise measurements of the heat capacities of the native and unfolded states of BPTI show that the heat capacity change upon unfolding strongly depends on temperature; its value is maximal at about 50 degrees C and diminishes as the temperature is increased. The temperature dependencies of the enthalpy and entropy changes upon BPTI unfolding were found to be similar to those normally observed for other small globular proteins. The stability of BPTI has been correlated with its structure. Study holds ProTherm entries: 2786, 2787, 2788, 2789, 2790, 2791 Extra Details: BPTI; heat capacity; scanning microcalorimetry; stability

Submission Details

ID: tmbxN9nC

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:19 p.m.

Version: 1

Publication Details
Makhatadze GI;Kim KS;Woodward C;Privalov PL,Protein Sci. (1993) Thermodynamics of BPTI folding. PMID:7507751
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3OTJ 2010-09-12T00:00:00+0000 2.15 A Crystal Structure of Trypsin Complexed with BPTI (Bovine Pancreatic Trypsin Inhibitor) by X-ray/Neutron Joint Refinement
2TPI 1981-10-26T00:00:00+0000 2.1 ON THE DISORDERED ACTIVATION DOMAIN IN TRYPSINOGEN. CHEMICAL LABELLING AND LOW-TEMPERATURE CRYSTALLOGRAPHY
5YW1 2017-11-28T00:00:00+0000 2.6 Crystal structure of full length NS3 protein (eD4NS2BNS3) in complex with Bovine Pancreatic Trypsin Inhibitor
1T8L 2004-05-13T00:00:00+0000 1.75 CRYSTAL STRUCTURE OF THE P1 MET BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX
1T8N 2004-05-13T00:00:00+0000 1.75 CRYSTAL STRUCTURE OF THE P1 THR BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX
1K6U 2001-10-17T00:00:00+0000 1.0 Crystal Structure of Cyclic Bovine Pancreatic Trypsin Inhibitor
1JV9 2001-08-28T00:00:00+0000 0 NMR Structure of BPTI Mutant G37A
3TGI 1998-07-15T00:00:00+0000 1.8 WILD-TYPE RAT ANIONIC TRYPSIN COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR (BPTI)
1JV8 2001-08-28T00:00:00+0000 0 NMR Structure of BPTI Mutant G37A
3BTM 1999-03-10T00:00:00+0000 1.8 THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
91.0 Pancreatic trypsin inhibitor P04815 BPT2_BOVIN
100.0 Pancreatic trypsin inhibitor P00974 BPT1_BOVIN
91.4 Pancreatic trypsin inhibitor P00975 IBPS_BOVIN