Reversible unfolding of dimeric phosphofructokinase-2 from Escherichia coli reveals a dominant role of inter-subunit contacts for stability.


Abstract

Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organization of Pfk-2 determines its stability, the reversible unfolding of the enzyme was characterized under equilibrium conditions by enzymatic activity, circular dichroism, fluorescence and hydrodynamic measurements. Pfk-2 undergoes a cooperative unfolding/dissociation process with the accumulation of an expanded and unstructured monomeric intermediate with a marginal stability and a large solvent accessibility with respect to the native dimer. Study holds ProTherm entries: 25001, 25002, 25003, 25004 Extra Details: 10 mM DTT was added in the experiment; Native to intermediate Reversible unfolding; Subunit interaction; Folding intermediate; Dimer monomer transition; Ribokinase superfamily; Bimolecular domain

Submission Details

ID: tU8XUF4V3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:55 p.m.

Version: 1

Publication Details
Baez M;Babul J,FEBS Lett. (2009) Reversible unfolding of dimeric phosphofructokinase-2 from Escherichia coli reveals a dominant role of inter-subunit contacts for stability. PMID:19465020
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3UMO 2012-11-14 1.7 Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Potassium
3UMP 2012-11-14 1.85 Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP
3CQD 2008-09-16 1.98 Structure of the tetrameric inhibited form of phosphofructokinase-2 from Escherichia coli
3N1C 2010-12-08 2.0 Crystal structure of the phosphofructokinase-2 from Escherichia coli in complex with fructose-6-phosphate
3UQD 2012-11-28 2.14 Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with substrates and products
3UQE 2012-11-21 2.2 Crystal structure of the Phosphofructokinase-2 mutant Y23D from Escherichia coli

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 ATP-dependent 6-phosphofructokinase isozyme 2 P06999 PFKB_ECOLI