Stability of proteins in the presence of polyols estimated from their guanidinium chloride-induced transition curves at different pH values and 25 degrees C.


Abstract

We have recently concluded from the heat-induced denaturation studies that polyols do not affect deltaG(D) degrees (the Gibbs free energy change (deltaG(D)) at 25 degrees C) of ribonuclease-A and lysozyme at physiological pH and temperature, and their stabilizing effect increases with decrease in pH. Since the estimation of deltaG(D) degrees of proteins from heat-induced denaturation curves requires a large extrapolation, the reliability of this procedure for the estimation of deltaG(D) degrees is always questionable, and so are conclusions drawn from such studies. This led us to measure deltaG(D) degrees of ribonuclease-A and lysozyme using a more accurate method, i.e., from their isothermal (25 degrees C) guanidinium chloride (GdmCl)-induced denaturations. We show that our earlier conclusions drawn from heat-induced denaturation studies are correct. Since the extent of unfolding of heat- and GdmCl-induced denatured states of these proteins is not identical, the extent of stabilization of the proteins by polyols against heat and GdmCl denaturations may also differ. We report that in spite of the differences in the structural nature of the heat- and GdmCl-denatured states of each protein, the extent of stabilization by a polyol is same. We also report that the functional dependence of deltaG(D) of proteins in the presence of polyols on denaturant concentration is linear through the full denaturant concentration range. Furthermore, polyols do not affect the secondary and tertiary structures of the native and GdmCl-denatured states. Study holds ProTherm entries: 20278, 20279, 20280, 20281, 20282, 20283, 20284, 20285, 20286, 20287, 20288, 20289, 20290, 20291, 20292, 20293, 20294, 20295, 20296, 20297, 20298, 20299, 20300, 20301, 20302, 20303, 20304, 20305, 20306, 20307, 20308, 20309, 20310, 20311, 20312, 20313, 20314, 20315, 20316, 20317, 20318, 20319, 20320, 20321, 20322, 20323, 20324, 20325, 20326, 20327, 20328, 20329, 20330, 20331, 20332, 20333, 20334, 20335, 20336, 20337, 20338, 20339, 20340, 20341, 20342, 20343, 20344, 20345, 20346, 20347, 20348, 20349, 20350, 20351, 20352, 20353, 20354, 20355, 20356, 20357, 20358, 20359, 20360, 20361, 20362, 20363, 20364, 20365, 20366, 20367, 20368, 20369, 20370, 20371, 20372, 20373, 20374, 20375, 20376, 20377, 20378, 20379, 20380, 20381, 20382, 20383, 20384, 20385, 20386, 20387, 20388, 20389, 20390, 20391, 20392, 20393, 20394, 20395, 20396, 20397, 20398, 20399, 20400, 20401, 20402, 20403, 20404, 20405, 20406, 20407, 20408, 20409, 20410, 20411, 20412, 20413, 20414, 20415, 20416, 20417, 20418, 20419, 20420, 20421, 20422, 20423, 20424, 20425, 20426, 20427, 20428, 20429, 20430, 20431, 20432, 20433, 20434, 20435, 20436, 20437, 20438, 20439, 20440, 20441, 20442, 20443, 20444, 20445, 20446, 20447, 20448, 20449, 20450, 20451, 20452, 20453, 20454, 20455, 20456, 20457, 20458, 20459, 20460, 20461, 20462, 20463, 20464, 20465 Extra Details: Protein stability; Guanidinium chloride denaturation; Protein structure; Polyol osmolyte; Ribonuclease-A; Lysozyme

Submission Details

ID: swLQWs8T4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:52 p.m.

Version: 1

Publication Details
Haque I;Islam A;Singh R;Moosavi-Movahedi AA;Ahmad F,Biophys. Chem. (2006) Stability of proteins in the presence of polyols estimated from their guanidinium chloride-induced transition curves at different pH values and 25 degrees C. PMID:16226834
Additional Information

Number of data points 403
Proteins Lysozyme C ; Ribonuclease pancreatic ; Ribonuclease pancreatic ; Lysozyme C
Unique complexes 2
Assays/Quantities/Protocols Experimental Assay: Cm details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: m details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: dG_H2O details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: Cm details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: m details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: dG_H2O details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: Cm pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: m pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm details:Additives xylitol (0.50 M), pH:1.9 ; Experimental Assay: m details:Additives xylitol (0.50 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives xylitol (0.50 M), pH:1.9 ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: m details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: Cm details:Additives sorbitol (1.10 M), pH:1.9 ; Experimental Assay: m details:Additives sorbitol (1.10 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives sorbitol (1.10 M), pH:1.9 ; Experimental Assay: Cm details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: m details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: dG_H2O details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: Cm pH:3.0, details:Additives xylitol-2.60M (0.50 M) ; Experimental Assay: m pH:3.0, details:Additives xylitol-2.60M (0.50 M) ; Experimental Assay: dG_H2O pH:3.0, details:Additives xylitol-2.60M (0.50 M) ; Experimental Assay: Cm pH:3.0, details:Additives xylitol-1.90M (0.50 M) ; Experimental Assay: m pH:3.0, details:Additives xylitol-1.90M (0.50 M) ; Experimental Assay: dG_H2O pH:3.0, details:Additives xylitol-1.90M (0.50 M) ; Experimental Assay: Cm pH:3.0, details:Additives xylitol-0.75M (0.50 M) ; Experimental Assay: m pH:3.0, details:Additives xylitol-0.75M (0.50 M) ; Experimental Assay: dG_H2O pH:3.0, details:Additives xylitol-0.75M (0.50 M) ; Experimental Assay: Cm details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: m details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: dG_H2O details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: Cm pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: m pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm details:Additives xylitol-3.30M (0.5 M), pH:1.9 ; Experimental Assay: m details:Additives xylitol-3.30M (0.5 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives xylitol-3.30M (0.5 M), pH:1.9 ; Experimental Assay: Cm details:Additives xylitol-1.40M (0,5 M), pH:1.9 ; Experimental Assay: m details:Additives xylitol-1.40M (0,5 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives xylitol-1.40M (0,5 M), pH:1.9 ; Experimental Assay: Cm pH:1.9, details:Additives Glycerol-3.20M (2.74 M) ; Experimental Assay: m pH:1.9, details:Additives Glycerol-3.20M (2.74 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Glycerol-3.20M (2.74 M) ; Experimental Assay: Cm details:Additives Glycerol-1.60M (2.74 M), pH:1.9 ; Experimental Assay: m details:Additives Glycerol-1.60M (2.74 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Glycerol-1.60M (2.74 M), pH:1.9 ; Experimental Assay: Cm pH:1.9, details:Additives sorbitol-3.90M (1.10 M) ; Experimental Assay: m pH:1.9, details:Additives sorbitol-3.90M (1.10 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives sorbitol-3.90M (1.10 M) ; Experimental Assay: Cm pH:1.9, details:Additives sorbitol-1.60M (1.10 M) ; Experimental Assay: m pH:1.9, details:Additives sorbitol-1.60M (1.10 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives sorbitol-1.60M (1.10 M) ; Experimental Assay: Cm pH:6.0, details:Additives Mannitol (1.0 M) ; Experimental Assay: dG_H2O pH:6.0, details:Additives Mannitol (1.0 M) ; Experimental Assay: Cm details:Additives Mannitol (1.0 M), pH:5.5 ; Experimental Assay: dG_H2O details:Additives Mannitol (1.0 M), pH:5.5 ; Experimental Assay: Cm details:Additives Mannitol (1.0 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Mannitol (1.0 M), pH:3.5 ; Experimental Assay: Cm details:Additives Mannitol (1.0 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Mannitol (1.0 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:3.5, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:2.0, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:3.5, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:2.0, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:3.5, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:2.0, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:6.0, details:Additives Adonitol (1.00 M) ; Experimental Assay: dG_H2O pH:6.0, details:Additives Adonitol (1.00 M) ; Experimental Assay: Cm pH:5.5, details:Additives Adonitol (1.00 M) ; Experimental Assay: dG_H2O pH:5.5, details:Additives Adonitol (1.00 M) ; Experimental Assay: Cm details:Additives Adonitol (1.00 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Adonitol (1.00 M), pH:3.5 ; Experimental Assay: Cm details:Additives Adonitol (1.00 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Adonitol (1.00 M), pH:2.0 ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:3.5 ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:5.5, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Adonitol (0.50 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.50 M), pH:3.5 ; Experimental Assay: Cm details:Additives Adonitol (0.50 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.50 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:3.5, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:2.0, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:6.0, details:Additives Xylitol (1.0 M) ; Experimental Assay: dG_H2O pH:6.0, details:Additives Xylitol (1.0 M) ; Experimental Assay: Cm details:Additives Xylitol (1.0 M), pH:5.5 ; Experimental Assay: dG_H2O details:Additives Xylitol (1.0 M), pH:5.5 ; Experimental Assay: Cm details:Additives Xylitol (1.0 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Xylitol (1.0 M), pH:3.5 ; Experimental Assay: Cm details:Additives Xylitol (1.0 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Xylitol (1.0 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:3.5, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:2.0, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:3.5 ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:2.0 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:3.5, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:2.0, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:5.5, details:Additives Glycerol (5.48 M) ; Experimental Assay: dG_H2O pH:5.5, details:Additives Glycerol (5.48 M) ; Experimental Assay: Cm pH:3.5, details:Additives Glycerol (5.48 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Glycerol (5.48 M) ; Experimental Assay: Cm details:Additives Glycerol (5.48 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Glycerol (5.48 M), pH:2.0 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:3.5, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm details:Additives Glycerol (4.10 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Glycerol (4.10 M), pH:2.0 ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:3.5 ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:3.5, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:2.0, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:3.5, details:Additives Sorbitol (2.20 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Sorbitol (2.20 M) ; Experimental Assay: Cm pH:2.0, details:Additives Sorbitol (2.20 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Sorbitol (2.20 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:3.5, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:2.0, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:3.5, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:3.5, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:2.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:2.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:5.5, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:5.5, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Sorbitol (0.55 M),, pH:3.5 ; Experimental Assay: dG_H2O details:Additives Sorbitol (0.55 M),, pH:3.5 ; Experimental Assay: Cm details:Additives Sorbitol (0.55 M),, pH:2.0 ; Experimental Assay: dG_H2O details:Additives Sorbitol (0.55 M),, pH:2.0 ; Experimental Assay: Cm pH:5.5, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:5.5, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives , pH:3.5 ; Experimental Assay: dG_H2O details:Additives , pH:3.5 ; Experimental Assay: Cm details:Additives , pH:2.0 ; Experimental Assay: dG_H2O details:Additives , pH:2.0 ; Experimental Assay: Cm pH:2.4, details:Additives Mannitol (1.0 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Mannitol (1.0 M) ; Experimental Assay: Cm details:Additives Mannitol (1.0 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Mannitol (1.0 M), pH:1.9 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:2.4, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:1.9, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:2.4, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:1.9, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:1.9, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Adonitol (1.00 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Adonitol (1.00 M) ; Experimental Assay: Cm pH:1.9, details:Additives Adonitol (1.00 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Adonitol (1.00 M) ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:6.0, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:6.0, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:2.4 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:2.4 ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:1.9 ; Experimental Assay: Cm pH:6.0, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:6.0, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:4.8, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:4.8, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives Adonitol (0.50 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Adonitol (0.50 M) ; Experimental Assay: Cm pH:1.9, details:Additives Adonitol (0.50 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Adonitol (0.50 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:1.9, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Xylitol (1.0 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Xylitol (1.0 M) ; Experimental Assay: Cm details:Additives Xylitol (1.0 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Xylitol (1.0 M), pH:1.9 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:2.4, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:1.9, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:6.0, details:Additives Xylitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:6.0, details:Additives Xylitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives Xylitol (0.50 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Xylitol (0.50 M) ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:1.9 ; Experimental Assay: m details:Additives Xylitol (0.50 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:1.9 ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:1.9, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Glycerol (5.48 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Glycerol (5.48 M) ; Experimental Assay: Cm pH:1.9, details:Additives Glycerol (5.48 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Glycerol (5.48 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:2.4, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:1.9, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (2.74 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (2.74 M) ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives Glycerol (2.74 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Glycerol (2.74 M) ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: m details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:2.4, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:1.9, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:2.4, details:Additives Sorbitol (2.20 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Sorbitol (2.20 M) ; Experimental Assay: Cm pH:1.9, details:Additives Sorbitol (2.20 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Sorbitol (2.20 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:2.4, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:1.9, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:2.4, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:2.4, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:1.9, details:Additives Sorbitol (1.10 M), ; Experimental Assay: m pH:1.9, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:1.9, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:6.0, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:6.0, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:4.8, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:4.8, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives Sorbitol (0.55 M), ; Experimental Assay: dG_H2O pH:2.4, details:Additives Sorbitol (0.55 M), ; Experimental Assay: Cm pH:1.9, details:Additives Sorbitol (0.55 M), ; Experimental Assay: dG_H2O pH:1.9, details:Additives Sorbitol (0.55 M), ; Experimental Assay: Cm pH:6.0, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:6.0, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:4.8, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:4.8, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives ; Experimental Assay: dG_H2O pH:2.4, details:Additives ; Experimental Assay: Cm pH:1.9, details:Additives ; Experimental Assay: dG_H2O pH:1.9, details:Additives
Libraries Mutations for sequence KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL ; Mutations for sequence KETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHFDASV
Sequence Assay Result Units