Stability of proteins in the presence of polyols estimated from their guanidinium chloride-induced transition curves at different pH values and 25 degrees C.


Abstract

We have recently concluded from the heat-induced denaturation studies that polyols do not affect deltaG(D) degrees (the Gibbs free energy change (deltaG(D)) at 25 degrees C) of ribonuclease-A and lysozyme at physiological pH and temperature, and their stabilizing effect increases with decrease in pH. Since the estimation of deltaG(D) degrees of proteins from heat-induced denaturation curves requires a large extrapolation, the reliability of this procedure for the estimation of deltaG(D) degrees is always questionable, and so are conclusions drawn from such studies. This led us to measure deltaG(D) degrees of ribonuclease-A and lysozyme using a more accurate method, i.e., from their isothermal (25 degrees C) guanidinium chloride (GdmCl)-induced denaturations. We show that our earlier conclusions drawn from heat-induced denaturation studies are correct. Since the extent of unfolding of heat- and GdmCl-induced denatured states of these proteins is not identical, the extent of stabilization of the proteins by polyols against heat and GdmCl denaturations may also differ. We report that in spite of the differences in the structural nature of the heat- and GdmCl-denatured states of each protein, the extent of stabilization by a polyol is same. We also report that the functional dependence of deltaG(D) of proteins in the presence of polyols on denaturant concentration is linear through the full denaturant concentration range. Furthermore, polyols do not affect the secondary and tertiary structures of the native and GdmCl-denatured states. Study holds ProTherm entries: 20278, 20279, 20280, 20281, 20282, 20283, 20284, 20285, 20286, 20287, 20288, 20289, 20290, 20291, 20292, 20293, 20294, 20295, 20296, 20297, 20298, 20299, 20300, 20301, 20302, 20303, 20304, 20305, 20306, 20307, 20308, 20309, 20310, 20311, 20312, 20313, 20314, 20315, 20316, 20317, 20318, 20319, 20320, 20321, 20322, 20323, 20324, 20325, 20326, 20327, 20328, 20329, 20330, 20331, 20332, 20333, 20334, 20335, 20336, 20337, 20338, 20339, 20340, 20341, 20342, 20343, 20344, 20345, 20346, 20347, 20348, 20349, 20350, 20351, 20352, 20353, 20354, 20355, 20356, 20357, 20358, 20359, 20360, 20361, 20362, 20363, 20364, 20365, 20366, 20367, 20368, 20369, 20370, 20371, 20372, 20373, 20374, 20375, 20376, 20377, 20378, 20379, 20380, 20381, 20382, 20383, 20384, 20385, 20386, 20387, 20388, 20389, 20390, 20391, 20392, 20393, 20394, 20395, 20396, 20397, 20398, 20399, 20400, 20401, 20402, 20403, 20404, 20405, 20406, 20407, 20408, 20409, 20410, 20411, 20412, 20413, 20414, 20415, 20416, 20417, 20418, 20419, 20420, 20421, 20422, 20423, 20424, 20425, 20426, 20427, 20428, 20429, 20430, 20431, 20432, 20433, 20434, 20435, 20436, 20437, 20438, 20439, 20440, 20441, 20442, 20443, 20444, 20445, 20446, 20447, 20448, 20449, 20450, 20451, 20452, 20453, 20454, 20455, 20456, 20457, 20458, 20459, 20460, 20461, 20462, 20463, 20464, 20465 Extra Details: Protein stability; Guanidinium chloride denaturation; Protein structure; Polyol osmolyte; Ribonuclease-A; Lysozyme

Submission Details

ID: swLQWs8T4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:52 p.m.

Version: 1

Publication Details
Haque I;Islam A;Singh R;Moosavi-Movahedi AA;Ahmad F,Biophys. Chem. (2006) Stability of proteins in the presence of polyols estimated from their guanidinium chloride-induced transition curves at different pH values and 25 degrees C. PMID:16226834
Additional Information

Study Summary

Number of data points 403
Proteins Lysozyme C ; Ribonuclease pancreatic ; Ribonuclease pancreatic ; Lysozyme C
Unique complexes 2
Assays/Quantities/Protocols Experimental Assay: Cm details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: m details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: dG_H2O details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: Cm details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: m details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: dG_H2O details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: Cm pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: m pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm details:Additives xylitol (0.50 M), pH:1.9 ; Experimental Assay: m details:Additives xylitol (0.50 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives xylitol (0.50 M), pH:1.9 ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: m details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: Cm details:Additives sorbitol (1.10 M), pH:1.9 ; Experimental Assay: m details:Additives sorbitol (1.10 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives sorbitol (1.10 M), pH:1.9 ; Experimental Assay: Cm details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: m details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: dG_H2O details:Additives xylitol (0.5 M), pH:3.0 ; Experimental Assay: Cm pH:3.0, details:Additives xylitol-2.60M (0.50 M) ; Experimental Assay: m pH:3.0, details:Additives xylitol-2.60M (0.50 M) ; Experimental Assay: dG_H2O pH:3.0, details:Additives xylitol-2.60M (0.50 M) ; Experimental Assay: Cm pH:3.0, details:Additives xylitol-1.90M (0.50 M) ; Experimental Assay: m pH:3.0, details:Additives xylitol-1.90M (0.50 M) ; Experimental Assay: dG_H2O pH:3.0, details:Additives xylitol-1.90M (0.50 M) ; Experimental Assay: Cm pH:3.0, details:Additives xylitol-0.75M (0.50 M) ; Experimental Assay: m pH:3.0, details:Additives xylitol-0.75M (0.50 M) ; Experimental Assay: dG_H2O pH:3.0, details:Additives xylitol-0.75M (0.50 M) ; Experimental Assay: Cm details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: m details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: dG_H2O details:Additives glycerol (2.74M),, pH:3.0 ; Experimental Assay: Cm pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: m pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:3.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm details:Additives xylitol-3.30M (0.5 M), pH:1.9 ; Experimental Assay: m details:Additives xylitol-3.30M (0.5 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives xylitol-3.30M (0.5 M), pH:1.9 ; Experimental Assay: Cm details:Additives xylitol-1.40M (0,5 M), pH:1.9 ; Experimental Assay: m details:Additives xylitol-1.40M (0,5 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives xylitol-1.40M (0,5 M), pH:1.9 ; Experimental Assay: Cm pH:1.9, details:Additives Glycerol-3.20M (2.74 M) ; Experimental Assay: m pH:1.9, details:Additives Glycerol-3.20M (2.74 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Glycerol-3.20M (2.74 M) ; Experimental Assay: Cm details:Additives Glycerol-1.60M (2.74 M), pH:1.9 ; Experimental Assay: m details:Additives Glycerol-1.60M (2.74 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Glycerol-1.60M (2.74 M), pH:1.9 ; Experimental Assay: Cm pH:1.9, details:Additives sorbitol-3.90M (1.10 M) ; Experimental Assay: m pH:1.9, details:Additives sorbitol-3.90M (1.10 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives sorbitol-3.90M (1.10 M) ; Experimental Assay: Cm pH:1.9, details:Additives sorbitol-1.60M (1.10 M) ; Experimental Assay: m pH:1.9, details:Additives sorbitol-1.60M (1.10 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives sorbitol-1.60M (1.10 M) ; Experimental Assay: Cm pH:6.0, details:Additives Mannitol (1.0 M) ; Experimental Assay: dG_H2O pH:6.0, details:Additives Mannitol (1.0 M) ; Experimental Assay: Cm details:Additives Mannitol (1.0 M), pH:5.5 ; Experimental Assay: dG_H2O details:Additives Mannitol (1.0 M), pH:5.5 ; Experimental Assay: Cm details:Additives Mannitol (1.0 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Mannitol (1.0 M), pH:3.5 ; Experimental Assay: Cm details:Additives Mannitol (1.0 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Mannitol (1.0 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:3.5, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:2.0, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:3.5, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:2.0, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:3.5, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:2.0, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:6.0, details:Additives Adonitol (1.00 M) ; Experimental Assay: dG_H2O pH:6.0, details:Additives Adonitol (1.00 M) ; Experimental Assay: Cm pH:5.5, details:Additives Adonitol (1.00 M) ; Experimental Assay: dG_H2O pH:5.5, details:Additives Adonitol (1.00 M) ; Experimental Assay: Cm details:Additives Adonitol (1.00 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Adonitol (1.00 M), pH:3.5 ; Experimental Assay: Cm details:Additives Adonitol (1.00 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Adonitol (1.00 M), pH:2.0 ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:3.5 ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:5.5, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Adonitol (0.50 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.50 M), pH:3.5 ; Experimental Assay: Cm details:Additives Adonitol (0.50 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.50 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:3.5, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:2.0, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:6.0, details:Additives Xylitol (1.0 M) ; Experimental Assay: dG_H2O pH:6.0, details:Additives Xylitol (1.0 M) ; Experimental Assay: Cm details:Additives Xylitol (1.0 M), pH:5.5 ; Experimental Assay: dG_H2O details:Additives Xylitol (1.0 M), pH:5.5 ; Experimental Assay: Cm details:Additives Xylitol (1.0 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Xylitol (1.0 M), pH:3.5 ; Experimental Assay: Cm details:Additives Xylitol (1.0 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Xylitol (1.0 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:3.5, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:2.0, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:3.5 ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:2.0 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:3.5, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:2.0, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:5.5, details:Additives Glycerol (5.48 M) ; Experimental Assay: dG_H2O pH:5.5, details:Additives Glycerol (5.48 M) ; Experimental Assay: Cm pH:3.5, details:Additives Glycerol (5.48 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Glycerol (5.48 M) ; Experimental Assay: Cm details:Additives Glycerol (5.48 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Glycerol (5.48 M), pH:2.0 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:3.5, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm details:Additives Glycerol (4.10 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Glycerol (4.10 M), pH:2.0 ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:5.5, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:3.5 ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:3.5 ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:2.0 ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:2.0 ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:3.5, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:2.0, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:3.5, details:Additives Sorbitol (2.20 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Sorbitol (2.20 M) ; Experimental Assay: Cm pH:2.0, details:Additives Sorbitol (2.20 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Sorbitol (2.20 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:3.5, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:3.5, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:2.0, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:2.0, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:5.5, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:3.5, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:3.5, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:2.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:2.0, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:5.5, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:5.5, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Sorbitol (0.55 M),, pH:3.5 ; Experimental Assay: dG_H2O details:Additives Sorbitol (0.55 M),, pH:3.5 ; Experimental Assay: Cm details:Additives Sorbitol (0.55 M),, pH:2.0 ; Experimental Assay: dG_H2O details:Additives Sorbitol (0.55 M),, pH:2.0 ; Experimental Assay: Cm pH:5.5, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:5.5, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives , pH:3.5 ; Experimental Assay: dG_H2O details:Additives , pH:3.5 ; Experimental Assay: Cm details:Additives , pH:2.0 ; Experimental Assay: dG_H2O details:Additives , pH:2.0 ; Experimental Assay: Cm pH:2.4, details:Additives Mannitol (1.0 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Mannitol (1.0 M) ; Experimental Assay: Cm details:Additives Mannitol (1.0 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Mannitol (1.0 M), pH:1.9 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:2.4, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:1.9, details:Additives Mannitol (0.75 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Mannitol (0.75 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:2.4, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:1.9, details:Additives Mannitol (0.50 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Mannitol (0.50 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:1.9, details:Additives Mannitol (0.25 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Mannitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Adonitol (1.00 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Adonitol (1.00 M) ; Experimental Assay: Cm pH:1.9, details:Additives Adonitol (1.00 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Adonitol (1.00 M) ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:6.0, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:6.0, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:2.4 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:2.4 ; Experimental Assay: Cm details:Additives Adonitol (0.75 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Adonitol (0.75 M), pH:1.9 ; Experimental Assay: Cm pH:6.0, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:6.0, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:4.8, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:4.8, details:Additives Adonitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives Adonitol (0.50 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Adonitol (0.50 M) ; Experimental Assay: Cm pH:1.9, details:Additives Adonitol (0.50 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Adonitol (0.50 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:1.9, details:Additives Adonitol (0.25 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Adonitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Xylitol (1.0 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Xylitol (1.0 M) ; Experimental Assay: Cm details:Additives Xylitol (1.0 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Xylitol (1.0 M), pH:1.9 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:2.4, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:1.9, details:Additives Xylitol (0.75 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Xylitol (0.75 M) ; Experimental Assay: Cm pH:6.0, details:Additives Xylitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:6.0, details:Additives Xylitol (0.50 M), buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives Xylitol (0.50 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Xylitol (0.50 M) ; Experimental Assay: Cm details:Additives Xylitol (0.50 M), pH:1.9 ; Experimental Assay: m details:Additives Xylitol (0.50 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Xylitol (0.50 M), pH:1.9 ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:1.9, details:Additives Xylitol (0.25 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Xylitol (0.25 M) ; Experimental Assay: Cm pH:2.4, details:Additives Glycerol (5.48 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Glycerol (5.48 M) ; Experimental Assay: Cm pH:1.9, details:Additives Glycerol (5.48 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Glycerol (5.48 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:2.4, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:1.9, details:Additives Glycerol (4.10 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Glycerol (4.10 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (2.74 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (2.74 M) ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:4.8, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives Glycerol (2.74 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Glycerol (2.74 M) ; Experimental Assay: Cm details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: m details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: dG_H2O details:Additives Glycerol (2.74 M), pH:1.9 ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:2.4, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:1.9, details:Additives Glycerol (1.37 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Glycerol (1.37 M) ; Experimental Assay: Cm pH:2.4, details:Additives Sorbitol (2.20 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Sorbitol (2.20 M) ; Experimental Assay: Cm pH:1.9, details:Additives Sorbitol (2.20 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Sorbitol (2.20 M) ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:2.4, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:2.4, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:1.9, details:Additives Sorbitol (1.65 M) ; Experimental Assay: dG_H2O pH:1.9, details:Additives Sorbitol (1.65 M) ; Experimental Assay: Cm pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:6.0, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:4.8, buffers:Cacodylic acid: 0.05 M, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:2.4, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:2.4, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:1.9, details:Additives Sorbitol (1.10 M), ; Experimental Assay: m pH:1.9, details:Additives Sorbitol (1.10 M), ; Experimental Assay: dG_H2O pH:1.9, details:Additives Sorbitol (1.10 M), ; Experimental Assay: Cm pH:6.0, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:6.0, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:4.8, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:4.8, details:Additives Sorbitol (0.55 M),, buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives Sorbitol (0.55 M), ; Experimental Assay: dG_H2O pH:2.4, details:Additives Sorbitol (0.55 M), ; Experimental Assay: Cm pH:1.9, details:Additives Sorbitol (0.55 M), ; Experimental Assay: dG_H2O pH:1.9, details:Additives Sorbitol (0.55 M), ; Experimental Assay: Cm pH:6.0, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:6.0, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:4.8, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: dG_H2O pH:4.8, details:Additives , buffers:Cacodylic acid: 0.05 M ; Experimental Assay: Cm pH:2.4, details:Additives ; Experimental Assay: dG_H2O pH:2.4, details:Additives ; Experimental Assay: Cm pH:1.9, details:Additives ; Experimental Assay: dG_H2O pH:1.9, details:Additives
Libraries Mutations for sequence KETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHFDASV ; Mutations for sequence KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
2IHL 1993-06-29T00:00:00+0000 1.4 LYSOZYME (E.C.3.2.1.17) (JAPANESE QUAIL)
6QMN 2019-02-07T00:00:00+0000 2.31 Crystal structure of a Ribonuclease A-Onconase chimera
1FBI 1995-01-19T00:00:00+0000 3.0 CRYSTAL STRUCTURE OF A CROSS-REACTION COMPLEX BETWEEN FAB F9.13.7 AND GUINEA-FOWL LYSOZYME
1GHL 1993-05-04T00:00:00+0000 2.1 THE THREE-DIMENSIONAL STRUCTURE OF PHEASANT AND GUINEA-FOWL EGG LYSOZYMES
1HHL 1993-05-04T00:00:00+0000 1.9 THE THREE-DIMENSIONAL STRUCTURE OF PHEASANT AND GUINEA-FOWL EGG LYSOZYMES
1JHL 1993-05-04T00:00:00+0000 2.4 THREE-DIMENSIONAL STRUCTURE OF A HETEROCLITIC ANTIGEN-ANTIBODY CROSS-REACTION COMPLEX
1BQL 1995-02-03T00:00:00+0000 2.6 STRUCTURE OF AN ANTI-HEL FAB FRAGMENT COMPLEXED WITH BOBWHITE QUAIL LYSOZYME
1DKJ 1996-01-10T00:00:00+0000 2.0 BOBWHITE QUAIL LYSOZYME
1DKK 1996-01-10T00:00:00+0000 1.9 BOBWHITE QUAIL LYSOZYME WITH NITRATE
135L 1993-06-10T00:00:00+0000 1.3 X-RAY STRUCTURE OF MONOCLINIC TURKEY EGG LYSOZYME AT 1.3 ANGSTROMS RESOLUTION

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Lysozyme C P00698 LYSC_CHICK
96.9 Lysozyme C P00700 LYSC_COLVI
96.9 Lysozyme C P00699 LYSC_CALCC
96.9 Lysozyme C Q7LZQ0 LYSC_CATWA
96.9 Lysozyme C Q7LZP9 LYSC_LOPIM
96.1 Lysozyme C Q7LZI3 LYSC_TRASA
95.3 Lysozyme C P00701 LYSC_COTJA
96.1 Lysozyme C P19849 LYSC_PAVCR
95.3 Lysozyme C P22910 LYSC_CHRAM
95.3 Lysozyme C Q7LZT2 LYSC_TRATE
95.2 Lysozyme C P00703 LYSC_MELGA
92.2 Lysozyme C P00704 LYSC_NUMME
93.0 Lysozyme C P24364 LYSC_LOPLE
94.6 Lysozyme C P24533 LYSC_SYRRE
93.2 Lysozyme C P00702 LYSC_PHACO
93.0 Lysozyme C P81711 LYSC_SYRSO
92.3 Lysozyme C P49663 LYSC_PHAVE
100.0 Ribonuclease pancreatic P61823 RNAS1_BOVIN
100.0 Ribonuclease pancreatic P61824 RNAS1_BISBI
96.8 Ribonuclease pancreatic P67926 RNAS1_CAPHI
96.8 Ribonuclease pancreatic P67927 RNAS1_SHEEP
95.2 Ribonuclease pancreatic P00657 RNAS1_BUBBU
96.0 Ribonuclease pancreatic P07847 RNAS1_AEPME
93.5 Ribonuclease pancreatic P07848 RNAS1_EUDTH
95.2 Ribonuclease pancreatic P00660 RNAS1_CONTA
92.7 Ribonuclease pancreatic P00668 RNAS1_ANTAM
90.3 Ribonuclease pancreatic P00662 RNAS1_GIRCA
96.0 Ribonuclease pancreatic Q29606 RNAS1_ORYLE