Differential scanning calorimetry of the unfolding of myosin subfragment 1, subfragment 2, and heavy meromyosin.


Abstract

The thermal unfolding of rabbit skeletal heavy meromyosin (HMM), myosin subfragment 1, and subfragment 2 has been studied by differential scanning calorimetry (DSC). Two distinct endotherms are observed in the DSC scan of heavy meromyosin. The first endotherm, with a Tm of 41 degrees C at pH 7.9 in 0.1 M KCl, is assigned to the unfolding of the subfragment 2 domain of HMM based on scans of isolated subfragment 2. The unfolding of the subfragment 2 domain is reversible both in the isolated form and in HMM. The unfolding of subfragment 2 in HMM can be fit as a single two-state transition with a delta Hvh and delta Hcal of 161 kcal/mol, indicating that subfragment 2 exists as a single domain in HMM. The unfolding of subfragment 2 is characterized by an extraordinarily large delta Cp of approximately 30,000 cal/(deg.mol). In the presence of nucleotides, the high-temperature HMM endotherm with a Tm of 48 degrees C shifts to higher temperature, indicating that this peak corresponds to the unfolding of the subfragment 1 domain. This assignment has been confirmed by comparison with isolated subfragment 1. The stabilizing effect of AMPPNP was significantly greater than that of ADP. The vanadate-trapped ADP species was slightly more stable than M.AMPPNP with a Tm at 58 degrees C. The unfolding of subfragment 1, both in the isolated form and in HMM, was irreversible. Only a single endotherm was noted in the DSC scans of the subfragment 1 domain of HMM and in freshly prepared subfragment 1 complexes.(ABSTRACT TRUNCATED AT 250 WORDS) Study holds ProTherm entries: 3676, 3677, 3678, 3679 Extra Details: endotherms; single two-state transition; stabilizing effect;,binding of nucleotides

Submission Details

ID: rUpx4kp93

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:22 p.m.

Version: 1

Publication Details
Shriver JW;Kamath U,Biochemistry (1990) Differential scanning calorimetry of the unfolding of myosin subfragment 1, subfragment 2, and heavy meromyosin. PMID:2185830
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
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2M0K 2013-10-30 3D Structure of Calmodulin and Calmodulin Binding Domain of Rat Olfactory Cyclic Nucleotide-Gated Ion Channel
1AK8 1997-09-17 NMR SOLUTION STRUCTURE OF CERIUM-LOADED CALMODULIN AMINO-TERMINAL DOMAIN (CE2-TR1C), 23 STRUCTURES
1NKF 1999-02-16 CALCIUM-BINDING PEPTIDE, NMR, 30 STRUCTURES
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1J7P 2001-11-07 Solution structure of Calcium calmodulin C-terminal domain
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2LL6 2012-05-16 Solution NMR structure of CaM bound to iNOS CaM binding domain peptide
2LLQ 2012-02-01 Solution nmr-derived structure of calmodulin c-lobe bound with er alpha peptide
2KNE 2009-11-24 Calmodulin wraps around its binding domain in the plasma membrane CA2+ pump anchored by a novel 18-1 motif
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2L53 2011-01-05 Solution NMR Structure of apo-calmodulin in complex with the IQ motif of Human Cardiac Sodium Channel NaV1.5
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2KUH 2010-03-09 Halothane binds to druggable sites in calcium-calmodulin: Solution structure of halothane-CaM C-terminal domain
2LQP 2012-06-06 NMR solution structure of the Ca2+-Calmodulin C-terminal domain in a complex with a peptide (NSCaTE) from the L-type Voltage-Gated Calcium Channel alpha1C subunit
2N8J 2016-10-12 Structure and 15N relaxation data of Calmodulin bound to the endothelial Nitric Oxide Synthase Calmodulin Binding Domain Peptide at Physiological Calcium Concentration
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3GOF 2010-03-31 1.45 Calmodulin bound to peptide from macrophage nitric oxide synthase
2W73 2009-05-12 1.45 High-resolution structure of the complex between calmodulin and a peptide from calcineurin A
2F3Y 2005-12-27 1.45 Calmodulin/IQ domain complex
3GP2 2010-04-07 1.46 Calmodulin bound to peptide from calmodulin kinase II (CaMKII)
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4J9Y 2013-03-27 1.51 Calcium-calmodulin complexed with the calmodulin binding domain from a small conductance potassium channel splice variant
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6DAD 2018-10-17 1.65 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex
4J9Z 2013-03-27 1.66 Calcium-calmodulin complexed with the calmodulin binding domain from a small conductance potassium channel splice variant and NS309
1FW4 2001-05-02 1.7 CRYSTAL STRUCTURE OF E. COLI FRAGMENT TR2C FROM CALMODULIN TO 1.7 A RESOLUTION
1MXE 2002-12-04 1.7 Structure of the Complex of Calmodulin with the Target Sequence of CaMKI
1CLL 1993-10-31 1.7 CALMODULIN STRUCTURE REFINED AT 1.7 ANGSTROMS RESOLUTION
5NIN 2017-12-06 1.7 Crystal Structure of AKAP79 calmodulin binding domain peptide in complex with Ca2+/Calmodulin
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3SG6 2012-06-20 1.7 Crystal Structure of Dimeric GCaMP2-LIA(linker 1)
1PRW 2003-10-10 1.7 Crystal structure of bovine brain Ca++ calmodulin in a compact form
1YR5 2006-07-04 1.7 1.7-A structure of calmodulin bound to a peptide from DAP kinase
5K8Q 2016-08-24 1.74 Crystal Structure of Calcium-loaded Calmodulin in complex with STRA6 CaMBP2-site peptide.
3EVU 2008-12-09 1.75 Crystal structure of Calcium bound dimeric GCAMP2
3UCW 2012-08-08 1.76 Structure of MG2+ bound N-Terminal domain of Calmodulin
5V02 2018-03-07 1.78 A positive allosteric modulator binding pocket in SK2 ion channels is shared by Riluzole and CyPPA
1IQ5 2001-09-26 1.8 Calmodulin/nematode CA2+/Calmodulin dependent kinase kinase fragment
4BW8 2014-01-15 1.8 Calmodulin with small bend in central helix
2Y4V 2011-12-28 1.8 CRYSTAL STRUCTURE OF HUMAN CALMODULIN IN COMPLEX WITH A DAP KINASE-1 MUTANT (W305Y) PEPTIDE
6MBA 2019-04-10 1.8 Crystal Structure of Human Nav1.4 CTerminal Domain in Complex with apo Calmodulin
4Q57 2014-07-23 1.8 Crystal structure of the plectin 1a actin-binding domain/N-terminal domain of calmodulin complex
3UCY 2012-08-08 1.8 Structure of Mg2+ bound N-terminal domain of calmodulin in the presence of Zn2+
1AHR 1997-06-16 1.8 CALMODULIN MUTANT WITH A TWO RESIDUE DELETION IN THE CENTRAL HELIX
5JQA 2017-10-11 1.8 CaM:RM20 complex
4BW7 2014-01-15 1.81 Calmodulin in complex with strontium
5JTH 2017-07-19 1.84 Crystal structure of E67A calmodulin - CaM:RM20 complex
3EK7 2008-12-16 1.85 Calcium-saturated GCaMP2 dimer
3BYA 2009-01-20 1.85 Structure of a Calmodulin Complex
2R28 2008-07-01 1.86 The complex Structure of Calmodulin Bound to a Calcineurin Peptide
6O5G 2019-08-07 1.89 Calmodulin in complex with isomalbrancheamide D
2WEL 2009-04-14 1.9 Crystal structure of SU6656-bound calcium/calmodulin-dependent protein kinase II delta in complex with calmodulin
3SG7 2012-06-20 1.9 Crystal Structure of GCaMP3-KF(linker 1)
6MUE 2019-05-08 1.9 Voltage-gated sodium channel NaV1.4 IQ domain in complex with Ca2+/Calmodulin
2HQW 2007-11-13 1.9 Crystal Structure of Ca2+/Calmodulin bound to NMDA Receptor NR1C1 peptide
3UCT 2012-08-08 1.9 Structure of Mn2+-bound N-terminal domain of calmodulin in the presence of Zn2+
4EHQ 2012-11-07 1.9 Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode
1UP5 2005-03-24 1.9 Chicken Calmodulin
3SJQ 2012-05-30 1.9 Crystal structure of a small conductance potassium channel splice variant complexed with calcium-calmodulin
5WBX 2017-12-20 1.9 Structural insights into the potency of SK/IK channel positive modulators
3SG5 2012-06-20 1.9 Crystal Structure of Dimeric GCaMP3-D380Y, QP(linker 1), LP(linker 2)
1ZUZ 2006-11-14 1.91 Calmodulin in complex with a mutant peptide from human DRP-1 kinase
5DSU 2017-01-11 1.93 Crystal structure of double mutant of N-domain of human calmodulin
2VAY 2008-09-16 1.94 Calmodulin complexed with CaV1.1 IQ peptide
3SUI 2012-09-12 1.95 Crystal structure of ca2+-calmodulin in complex with a trpv1 c-terminal peptide
3WFN 2013-08-28 1.95 Crystal Structure of Nav1.6 IQ motif in complex with apo-CaM
4Q5U 2014-09-03 1.95 Structure of calmodulin bound to its recognition site from calcineurin
6EEB 2019-08-07 1.96 Calmodulin in complex with malbrancheamide
2BCX 2006-10-31 2.0 Crystal structure of calmodulin in complex with a ryanodine receptor peptide
3EKH 2008-12-16 2.0 Calcium-saturated GCaMP2 T116V/K378W mutant monomer
1IWQ 2003-03-11 2.0 Crystal Structure of MARCKS calmodulin binding domain peptide complexed with Ca2+/Calmodulin
2BE6 2005-11-15 2.0 2.0 A crystal structure of the CaV1.2 IQ domain-Ca/CaM complex
5J03 2016-09-07 2.0 Crystal Structure of a chimeric Kv7.2 - Kv7.3 proximal C-terminal Domain in Complex with Calmodulin
1CDL 1994-08-31 2.0 TARGET ENZYME RECOGNITION BY CALMODULIN: 2.4 ANGSTROMS STRUCTURE OF A CALMODULIN-PEPTIDE COMPLEX
3SG2 2012-06-20 2.0 Crystal Structure of GCaMP2-T116V,D381Y
1CM1 1998-03-04 2.0 MOTIONS OF CALMODULIN-SINGLE-CONFORMER REFINEMENT
6HCS 2018-12-05 2.0 Crystal structure of CaM-peptide complex containing AzF at position 108
4RJD 2015-08-26 2.0 TFP bound in alternate orientations to calcium-saturated Calmodulin C-Domains
6DAE 2018-10-17 2.0 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex
1LIN 1996-03-08 2.0 CALMODULIN COMPLEXED WITH TRIFLUOPERAZINE (1:4 COMPLEX)
1CM4 1998-03-04 2.0 Motions of calmodulin-four-conformer refinement
2I08 2007-08-21 2.0 Solvation effect in conformational changes of EF-hand proteins: X-ray structure of Ca2+-saturated double mutant Q41L-K75I of N-domain of calmodulin
4HEX 2013-03-06 2.0 A novel conformation of calmodulin
3EVR 2008-12-09 2.0 Crystal structure of Calcium bound monomeric GCAMP2
1WRZ 2006-03-21 2.0 Calmodulin complexed with a peptide from a human death-associated protein kinase
1CDM 1994-08-31 2.0 MODULATION OF CALMODULIN PLASTICITY IN MOLECULAR RECOGNITION ON THE BASIS OF X-RAY STRUCTURES
4QNH 2014-08-06 2.02 Calcium-calmodulin (T79D) complexed with the calmodulin binding domain from a small conductance potassium channel SK2-a
4OY4 2015-02-18 2.03 calcium-free CaMPARI v0.2
3IFK 2009-12-15 2.03 Crystal Structure Of Calcium-Saturated Calmodulin N-terminal Domain Fragment, Residues 1-90
1NIW 2003-02-18 2.05 Crystal structure of endothelial nitric oxide synthase peptide bound to calmodulin
4M1L 2014-05-07 2.1 Complex of IQCG and Ca2+-bound CaM
3G43 2009-03-03 2.1 Crystal structure of the calmodulin-bound Cav1.2 C-terminal regulatory domain dimer
3SG3 2012-06-20 2.1 Crystal Structure of GCaMP3-D380Y
1YRT 2006-01-17 2.1 Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin
1OOJ 2003-03-25 2.11 Structural genomics of Caenorhabditis elegans : Calmodulin
1XA5 2004-12-21 2.12 Structure of Calmodulin in complex with KAR-2, a bis-indol alkaloid
2V01 2007-08-14 2.15 Recombinant vertebrate calmodulin complexed with Pb
6N5W 2019-03-13 2.15 Crystal structure of the Ca2+/CaM complex with independent peptides of Kv7.4 (KCNQ4) A & B domains
2F2O 2006-02-21 2.17 Structure of calmodulin bound to a calcineurin peptide: a new way of making an old binding mode
4DCK 2012-06-27 2.2 Crystal structure of the C-terminus of voltage-gated sodium channel in complex with FGF13 and CaM
6CZQ 2018-08-01 2.2 A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model
6B8N 2018-03-14 2.2 Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak
6B8P 2018-03-14 2.2 Crystal Structure of the Mg2+/CaM:Kv7.4 (KCNQ4) AB domain complex
4I2Y 2013-07-24 2.2 Crystal Structure of the genetically encoded calcium indicator RGECO1
4DBP 2013-01-30 2.2 Myosin VI nucleotide-free (MDINSERT2) D179Y crystal structure
3CLN 1988-07-16 2.2 STRUCTURE OF CALMODULIN REFINED AT 2.2 ANGSTROMS RESOLUTION
3L9I 2010-12-08 2.2 Myosin VI nucleotide-free (mdinsert2) L310G mutant crystal structure
2X51 2011-01-26 2.2 M6 delta Insert1
2COL 2006-01-24 2.2 Crystal structure analysis of CyaA/C-Cam with Pyrophosphate
1ZOT 2005-08-09 2.2 crystal structure analysis of the CyaA/C-Cam with PMEAPP
2V02 2007-08-14 2.2 Recombinant vertebrate calmodulin complexed with Ba
2X0G 2010-01-26 2.2 X-RAY STRUCTURE OF A DAP-KINASE CALMODULIN COMPLEX
4CLN 1992-07-15 2.2 STRUCTURE OF A RECOMBINANT CALMODULIN FROM DROSOPHILA MELANOGASTER REFINED AT 2.2-ANGSTROMS RESOLUTION
2YGG 2011-09-28 2.23 Complex of CaMBR and CaM
5DBR 2017-02-22 2.25 Ca2+ CaM with human cardiac Na+ channel (NaV1.5) inactivation gate
4L79 2014-01-29 2.3 Crystal Structure of nucleotide-free Myosin 1b residues 1-728 with bound Calmodulin
3DVK 2008-11-04 2.3 Crystal Structure of Ca2+/CaM-CaV2.3 IQ domain complex
1QIW 2000-03-28 2.3 Calmodulin complexed with N-(3,3,-diphenylpropyl)-N'-[1-R-(3,4-bis-butoxyphenyl)-ethyl]-propylenediamine (DPD)
6B8L 2018-03-14 2.3 Crystal Structure of the Apo/CaM:Kv7.4 (KCNQ4) AB Domain Complex
5WC5 2017-12-20 2.3 Structural insights into the potency of SK/IK channel positive modulators
3BXL 2008-03-25 2.3 Crystal structure of the R-type calcium channeL (CaV2.3) IQ domain and CA2+calmodulin complex
1L7Z 2003-09-16 2.3 Crystal structure of Ca2+/Calmodulin complexed with myristoylated CAP-23/NAP-22 peptide
2VB6 2007-12-11 2.3 Myosin VI (MD-insert2-CaM, Delta Insert1) Post-rigor state (crystal form 2)
6B8M 2018-03-14 2.3 Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 1 mM CaCl2 soak
5WSV 2017-06-07 2.33 Crystal structure of Myosin VIIa IQ5 in complex with Ca2+-CaM
4UPU 2014-08-20 2.34 Crystal structure of IP3 3-K calmodulin binding region in complex with Calmodulin
3DVE 2008-11-04 2.35 Crystal Structure of Ca2+/CaM-CaV2.2 IQ domain complex
3O77 2010-09-29 2.35 The structure of Ca2+ Sensor (Case-16)
1Y6W 2005-03-01 2.4 Trapped intermediate of calmodulin
6DAF 2018-10-17 2.4 2.4 Angstrom crystal structure of the F141L Ca/CaM:CaV1.2 IQ domain complex
3EWT 2009-10-20 2.4 Crystal Structure of calmodulin complexed with a peptide
2VAS 2007-12-11 2.4 Myosin VI (MD-insert2-CaM, Delta-Insert1) Post-rigor state
2BKH 2005-06-07 2.4 Myosin VI nucleotide-free (MDInsert2) crystal structure
3SG4 2012-06-20 2.4 Crystal Structure of GCaMP3-D380Y, LP(linker 2)
4PJJ 2015-04-29 2.4 MYOSIN VI (MD-INSERT2-CAM, DELTA-INSERT1) post-rigor state - long soaking with PO4
1CTR 1994-12-20 2.45 DRUG BINDING BY CALMODULIN: CRYSTAL STRUCTURE OF A CALMODULIN-TRIFLUOPERAZINE COMPLEX
2FOT 2006-09-05 2.45 Crystal structure of the complex between calmodulin and alphaII-spectrin
3WLC 2014-01-22 2.49 Crystal structure of dimeric GCaMP6m
3HR4 2009-09-08 2.5 Human iNOS Reductase and Calmodulin Complex
2IX7 2006-12-13 2.5 Structure of apo-calmodulin bound to unconventional myosin V
1YRU 2005-09-27 2.5 Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin and 1mM calcium chloride
6ALE 2018-08-08 2.5 A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model
6DAH 2018-10-17 2.5 2.5 Angstrom crystal structure of the N97S CaM mutant
4ZLK 2016-05-04 2.5 Crystal structure of mouse myosin-5a in complex with calcium-bound calmodulin
1QX5 2004-08-31 2.54 Crystal structure of apoCalmodulin
3BXK 2008-03-25 2.55 Crystal structure of the P/Q-type calcium channel (CaV2.1) IQ domain and CA2+calmodulin complex
3OXQ 2010-11-03 2.55 Crystal Structure of Ca2+/CaM-CaV1.2 pre-IQ/IQ domain complex
5H7D 2017-06-28 2.57 Crystal structure of the YgjG-protein A-Zpa963-calmodulin complex
3EVV 2008-12-09 2.6 Crystal Structure of Calcium bound dimeric GCAMP2 (#2)
3DVM 2008-11-04 2.6 Crystal Structure of Ca2+/CaM-CaV2.1 IQ domain complex
3EWV 2009-10-20 2.6 Crystal Structure of calmodulin complexed with a peptide
2F2P 2006-02-21 2.6 Structure of calmodulin bound to a calcineurin peptide: a new way of making an old binding mode
3KF9 2011-01-26 2.6 Crystal structure of the SdCen/skMLCK complex
4ANJ 2012-10-17 2.6 MYOSIN VI (MDinsert2-GFP fusion) PRE-POWERSTROKE STATE (MG.ADP.AlF4)
4GOW 2012-12-12 2.6 Crystal Structure of Ca2+/CaM:Kv7.4 (KCNQ4) B helix complex
3O78 2010-09-29 2.6 The structure of Ca2+ Sensor (Case-12)
4DBQ 2013-01-30 2.6 MYOSIN VI D179Y (MD-INSERT2-CAM, DELTA-INSERT1) post-rigor state
6B8Q 2018-03-14 2.6 Crystal Structure of the Mg2+/CaM:Kv7.5 (KCNQ5) AB domain complex
1QIV 2000-03-28 2.64 CALMODULIN COMPLEXED WITH N-(3,3,-DIPHENYLPROPYL)-N'-[1-R-(3,4-BIS-BUTOXYPHENYL)-ETHYL]-PROPYLENEDIAMINE (DPD), 1:2 COMPLEX
3EK4 2008-12-16 2.65 Calcium-saturated GCaMP2 Monomer
5COC 2016-03-30 2.67 Fusion protein of human calmodulin and B4 domain of protein A from staphylococcal aureus
6MUD 2019-05-08 2.69 Voltage-gated sodium channel NaV1.5 C-terminal domain in complex with Ca2+/Calmodulin
4E53 2013-03-20 2.69 Calmodulin and Nm peptide complex
4E50 2013-03-20 2.7 Calmodulin and Ng peptide complex
3WLD 2014-01-22 2.7 Crystal structure of monomeric GCaMP6m
3GN4 2009-09-08 2.7 Myosin lever arm
6K4K 2019-07-24 2.71 Crystal structure of SidJ-CaM binary complex at 2.71 A
4BYF 2014-03-26 2.74 Crystal structure of human Myosin 1c in complex with calmodulin in the pre-power stroke state
1A29 1998-09-16 2.74 CALMODULIN COMPLEXED WITH TRIFLUOPERAZINE (1:2 COMPLEX)
1K90 2002-01-23 2.75 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin and 3' deoxy-ATP
3EKJ 2008-12-16 2.8 Calcium-free GCaMP2 (calcium binding deficient mutant)
4OVN 2014-12-03 2.8 Voltage-gated Sodium Channel 1.5 (Nav1.5) C-terminal domain in complex with Calmodulin poised for activation
3EK8 2008-12-16 2.8 Calcium-saturated GCaMP2 T116V/G87R mutant monomer
3DVJ 2008-11-04 2.8 Crystal Structure of Ca2+/CaM-CaV2.2 IQ domain (without cloning artifact, HM to TV) complex
5HIT 2016-09-14 2.85 Crystal Structure Analysis of Ca2+-calmodulin and a C-terminal EAG1 channel fragment
4V0C 2014-11-05 2.86 Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin
1DEG 1994-05-31 2.9 THE LINKER OF DES-GLU84 CALMODULIN IS BENT AS SEEN IN THE CRYSTAL STRUCTURE
2BKI 2005-06-07 2.9 Myosin VI nucleotide-free (MDinsert2-IQ) crystal structure
1K93 2002-01-23 2.95 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin
6K4L 2019-07-24 2.95 Crystal structure of Se-labelled SidJ complex with CaM at 2.95 A
3J41 2013-07-31 25.0 Pseudo-atomic model of the Aquaporin-0/Calmodulin complex derived from electron microscopy
5WSU 2017-06-07 3.0 Crystal structure of Myosin VIIa IQ5-SAH in complex with apo-CaM
4IL1 2013-10-02 3.0 Crystal Structure of the Rat Calcineurin
1S26 2004-04-13 3.0 Structure of Anthrax Edema Factor-Calmodulin-alpha,beta-methyleneadenosine 5'-triphosphate Complex Reveals an Alternative Mode of ATP Binding to the Catalytic Site
4UMO 2014-11-05 3.0 Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin
4JPZ 2014-04-16 3.02 Voltage-gated sodium channel 1.2 C-terminal domain in complex with FGF13U and Ca2+/calmodulin
1QX7 2004-08-31 3.09 Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel
6K4R 2019-07-24 3.11 Crystal structure of SidJ-CaM-AMP ternary complex at 3.11 A
5V7X 2018-02-28 3.14 Crystal Structure of Myosin 1b residues 1-728 with bound sulfate and Calmodulin
5I0I 2016-09-07 3.15 Crystal structure of myosin X motor domain with 2IQ motifs in pre-powerstroke state
1XFX 2005-05-03 3.2 Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 10 millimolar exogenously added calcium chloride
6C1D 2018-01-31 3.2 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
1SK6 2004-06-08 3.2 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin, 3',5' cyclic AMP (cAMP), and pyrophosphate
1XFZ 2005-05-03 3.25 Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 1 millimolar exogenously added calcium chloride
1XFY 2005-05-03 3.3 Crystal structure of anthrax edema factor (EF) in complex with calmodulin
6MC9 2019-04-10 3.3 Crystal Structure of Human Nav1.4 C-Terminal (1599-1754) domain in complex with calcium-bound calmodulin
6O20 2019-04-24 3.3 Cryo-EM structure of TRPV5 with calmodulin bound
1PK0 2004-02-10 3.3 Crystal Structure of the EF3-CaM complexed with PMEApp
1XFV 2005-05-03 3.35 Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3' deoxy-ATP
1XFU 2005-05-03 3.35 Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex with calmodulin
1XFW 2005-05-03 3.4 Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3'5' cyclic AMP (cAMP)
6CNM 2018-05-02 3.4 Cryo-EM structure of the human SK4/calmodulin channel complex
4R8G 2014-12-03 3.5 Crystal Structure of Myosin-1c tail in complex with Calmodulin
6CNN 2018-05-02 3.5 Cryo-EM structure of the human SK4/calmodulin channel complex in the Ca2+ bound state I
1LVC 2002-12-04 3.6 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin and 2' deoxy, 3' anthraniloyl ATP
1Y0V 2005-05-03 3.6 Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate
6E2G 2018-08-22 3.6 Cryo-EM structure of rat TRPV6 in complex with Calmodulin
5VMS 2017-06-07 3.7 CryoEM structure of Xenopus KCNQ1 channel
5K7L 2016-08-17 3.78 Single particle cryo-EM structure of the voltage-gated K+ channel Eag1 bound to the channel inhibitor calmodulin
6C1G 2018-01-31 3.8 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
4JQ0 2014-05-21 3.84 Voltage-gated sodium channel 1.5 C-terminal domain in complex with FGF12B and Ca2+/calmodulin
6C1H 2018-01-31 3.9 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
6E2F 2018-08-22 3.9 Cryo-EM structure of human TRPV6 in complex with Calmodulin
6S5T 2019-07-24 4.15 Legionella pneumophila SidJ-Human calmodulin complex
6DMW 2018-10-24 4.4 Calmodulin-bound full-length rbTRPV5
6BNV 2018-01-10 4.6 CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine
6CNO 2018-05-02 4.7 Cryo-EM structure of the human SK4/calmodulin channel complex in the Ca2+ bound state II

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 B Calmodulin P20689 MYLK2_RAT
100.0 B Calmodulin P07313 MYLK2_RABIT
100.0 B Calmodulin Q8VCR8 MYLK2_MOUSE
100.0 B Calmodulin Q9H1R3 MYLK2_HUMAN
100.0 B Calmodulin A4IFM7 MYLK2_BOVIN
93.5 A Calmodulin P18061 CALM_TRYCR
93.5 A Calmodulin P69098 CALM_TRYBG
93.5 A Calmodulin P69097 CALM_TRYBB
100.0 A Calmodulin P62184 CALM_RENRE
100.0 A Calmodulin P11121 CALM_PYUSP
100.0 A Calmodulin P02595 CALM_PATSP
92.3 A Calmodulin P53440 CALMF_NAEGR
94.2 A Calmodulin P11118 CALM_EUGGR
100.0 A Calmodulin P02594 CALM_ELEEL
91.4 A Calmodulin P05932 CALMB_ARBPU
96.5 A Calmodulin Q9HFY6 CALM_BLAEM
100.0 A Calmodulin Q40302 CALM_MACPY
91.9 A Calmodulin P62150 CALM_ORYLA
95.7 A Calmodulin O02367 CALM_CIOIN
91.2 A Calmodulin P27166 CALM_STYLE
91.2 A Calmodulin O97341 CALM_SUBDO
91.2 A Calmodulin A8I1Q0 CALM_HETTR
92.6 A Calmodulin P02598 CALM_TETPY
92.6 A Calmodulin A3E4D8 CALM_PROMN
92.6 A Calmodulin A3E3H0 CALM_PFIPI
92.6 A Calmodulin A3E4F9 CALM_KARVE
92.6 A Calmodulin A4UHC0 CALM_ALEFU
92.6 A Calmodulin O96081 CALMB_HALRO
92.6 A Calmodulin P62146 CALMA_ARBPU
98.3 A Calmodulin Q95NR9 CALM_METSE
97.4 A Calmodulin Q7T3T2 CALM_EPIAK
98.3 A Calmodulin P0DP35 CAM2B_XENLA
98.3 A Calmodulin P0DP34 CAM2A_XENLA
98.3 A Calmodulin P62151 CALM_TETCF
98.3 A Calmodulin P21251 CALM_STIJA
98.3 A Calmodulin Q6YNX6 CALM_SHEEP
98.3 A Calmodulin P62160 CALM_RABIT
98.3 A Calmodulin Q5RAD2 CALM_PONAB
98.3 A Calmodulin Q71UH6 CALM_PERFV
98.3 A Calmodulin P62156 CALM_ONCSP
98.3 A Calmodulin Q6PI52 CALM_DANRE
98.3 A Calmodulin Q6IT78 CALM_CTEID
98.3 A Calmodulin P62149 CALM_CHICK
98.3 A Calmodulin P62157 CALM_BOVIN
98.3 A Calmodulin P62144 CALM_ANAPL
98.3 A Calmodulin P0DP31 CALM3_RAT
98.3 A Calmodulin P0DP28 CALM3_MOUSE
98.3 A Calmodulin P0DP25 CALM3_HUMAN
98.3 A Calmodulin P0DP30 CALM2_RAT
98.3 A Calmodulin P0DP27 CALM2_MOUSE
98.3 A Calmodulin P0DP24 CALM2_HUMAN
99.1 A Calmodulin Q9UB37 CALM2_BRALA
98.3 A Calmodulin P0DP33 CALM1_XENLA
98.3 A Calmodulin P0DP29 CALM1_RAT
98.3 A Calmodulin P0DP26 CALM1_MOUSE
98.3 A Calmodulin P0DP23 CALM1_HUMAN
98.0 A Calmodulin Q8STF0 CALM_STRIE
99.3 A Calmodulin P62154 CALM_LOCMI
99.3 A Calmodulin P62152 CALM_DROME
99.3 A Calmodulin P62145 CALM_APLCA
99.3 A Calmodulin P62153 CALMA_HALRO
99.3 A Calmodulin P62148 CALM1_BRALA
99.3 A Calmodulin P62147 CALM1_BRAFL
98.6 A Calmodulin O16305 CALM_CAEEL