Folding of a mutant maltose-binding protein of Escherichia coli which forms inclusion bodies.


Abstract

The maltose-binding protein (MalE) of Escherichia coli is the periplasmic component of the transport system for malto-oligosaccharides. We have examined the characteristics of a Mal- mutant of malE corresponding to the double substitution Gly32 --> Asp/Ile33 --> Pro, MalE31, previously obtained by random mutagenesis. In vivo, the MalE31 precursor is efficiently processed, but the mature protein forms inclusion bodies in the periplasm. Furthermore, the accumulation of insoluble MalE31 is independent of its cellular localization; MalE31 lacking its signal sequence forms inclusion bodies in the cytoplasm. The native MalE31 protein can be purified by affinity chromatography from inclusion bodies after denaturation by 8 M urea. The renatured protein exhibits full maltose binding affinity (Kd= 9 x 10(-7) M), suggesting that its folded structure is similar to that of the wild-type protein. Unfolding/refolding experiments show that MalE31 is less stable (-5. 5 kcal/mol) than the wild-type protein (-9.5 kcal/mol) and that folding intermediates have a high tendency to form aggregates. In conclusion, the observed phenotype of cells expressing malE31 can be explained by a defective folding pathway of the protein. Study holds ProTherm entries: 5252, 5253 Extra Details: periplasm; cellular localization; signal sequence;,binding affinity; folded structure; folding pathway

Submission Details

ID: rSQC62sQ3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:29 p.m.

Version: 1

Publication Details
Betton JM;Hofnung M,J. Biol. Chem. (1996) Folding of a mutant maltose-binding protein of Escherichia coli which forms inclusion bodies. PMID:8626487
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
5ZR0 2018-08-22 Solution structure of peptidyl-prolyl cis/trans isomerase domain of Trigger Factor in complex with MBP
2N45 2015-07-01 EC-NMR Structure of Escherichia coli Maltose-binding protein Determined by Combining Evolutionary Couplings (EC) and Sparse NMR Data with a second set of RDC data simulated for an alternative alignment tensor. Northeast Structural Genomics Consortium target ER690
2MV0 2014-12-10 Solution NMR Structure of Maltose-binding protein from Escherichia coli, Northeast Structural Genomics Consortium (NESG) Target ER690
1EZP 2001-05-08 GLOBAL FOLD OF MALTODEXTRIN BINDING PROTEIN COMPLEXED WITH BETA-CYCLODEXTRIN USING PEPTIDE ORIENTATIONS FROM DIPOLAR COUPLINGS
2D21 2006-03-07 NMR Structure of stereo-array isotope labelled (SAIL) maltodextrin-binding protein (MBP)
1EZO 2001-05-03 GLOBAL FOLD OF MALTODEXTRIN BINDING PROTEIN COMPLEXED WITH BETA-CYCLODEXTRIN
5JTR 2016-08-24 The structure of chaperone SecB in complex with unstructured MBP binding site e
2N44 2015-07-01 EC-NMR Structure of Escherichia coli Maltose-binding protein Determined by Combining Evolutionary Couplings (EC) and Sparse NMR Data. Northeast Structural Genomics Consortium target ER690
2H25 2006-10-31 Solution Structure of Maltose Binding Protein complexed with beta-cyclodextrin
2KLF 2009-10-06 PERE NMR structure of maltodextrin-binding protein
5JTQ 2016-08-24 The structure of chaperone SecB in complex with unstructured MBP binding site d
2V93 2007-11-06 EQUILLIBRIUM MIXTURE OF OPEN AND PARTIALLY-CLOSED SPECIES IN THE APO STATE OF MALTODEXTRIN-BINDING PROTEIN BY PARAMAGNETIC RELAXATION ENHANCEMENT NMR
4EXK 2012-08-08 1.28 A chimera protein containing MBP fused to the C-terminal domain of the uncharacterized protein STM14_2015 from Salmonella enterica
3Q27 2011-06-01 1.3 Cyrstal structure of human alpha-synuclein (32-57) fused to maltose binding protein (MBP)
5M13 2017-11-15 1.37 Synthetic nanobody in complex with MBP
5H7Q 2017-02-15 1.45 Crystal structure of human MNDA PYD domain with MBP tag
4IRL 2013-08-07 1.47 X-ray structure of the CARD domain of zebrafish GBP-NLRP1 like protein
3MP6 2011-05-04 1.48 Complex Structure of Sgf29 and dimethylated H3K4
3KJT 2010-02-09 1.5 Stimulation of the maltose transporter by a mutant sucrose binding protein gives insights into ABC transporter coupling
3O3U 2010-10-13 1.5 Crystal Structure of Human Receptor for Advanced Glycation Endproducts (RAGE)
3Q26 2011-06-01 1.54 Cyrstal structure of human alpha-synuclein (10-42) fused to maltose binding protein (MBP)
3SEW 2011-09-21 1.55 Zn-mediated Polymer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)
4WMU 2015-05-06 1.55 STRUCTURE OF MBP-MCL1 BOUND TO ligand 2 AT 1.55A
3Q28 2011-06-01 1.6 Cyrstal structure of human alpha-synuclein (58-79) fused to maltose binding protein (MBP)
5M14 2017-11-15 1.6 Synthetic nanobody in complex with MBP
4EDQ 2013-03-20 1.64 MBP-fusion protein of myosin-binding protein c residues 149-269
5WQ6 2017-02-15 1.65 Crystal Structure of hMNDA-PYD with MBP tag
1ANF 1997-12-24 1.67 MALTODEXTRIN BINDING PROTEIN WITH BOUND MALTOSE
5VAW 2018-03-28 1.69 Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075
4XHS 2016-01-27 1.7 Crystal structure of human NLRP12 PYD domain and implication in homotypic interaction
4RG5 2015-07-01 1.7 Crystal Structure of S. Pombe SMN YG-Dimer
4MBP 1998-02-25 1.7 MALTODEXTRIN BINDING PROTEIN WITH BOUND MALTETROSE
5AZ8 2016-01-13 1.7 Crystal structure of MBP-Tom20 fusion protein tethered with ALDH presequence via a disulfide bond
3MBP 1997-12-24 1.7 MALTODEXTRIN-BINDING PROTEIN WITH BOUND MALTOTRIOSE
4PE2 2015-01-14 1.72 MBP PilA1 CD160
6N84 2019-07-10 1.75 MBP-fusion protein of transducin-alpha residues 327-350
3JYR 2010-02-16 1.75 Crystal structures of the GacH receptor of Streptomyces glaucescens GLA.O in the unliganded form and in complex with acarbose and an acarbose homolog. Comparison with acarbose-loaded maltose binding protein of Salmonella typhimurium.
4QVH 2014-12-17 1.75 Crystal structure of the essential Mycobacterium tuberculosis phosphopantetheinyl transferase PptT, solved as a fusion protein with maltose binding protein
3G7W 2009-06-23 1.75 Islet Amyloid Polypeptide (IAPP or Amylin) Residues 1 to 22 fused to Maltose Binding Protein
4RWF 2015-05-20 1.76 Crystal structure of the CLR:RAMP2 extracellular domain heterodimer with bound adrenomedullin
3W15 2013-07-03 1.8 Structure of peroxisomal targeting signal 2 (PTS2) of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase in complex with Pex7p and Pex21p
1LLS 2002-09-18 1.8 CRYSTAL STRUCTURE OF UNLIGANDED MALTOSE BINDING PROTEIN WITH XENON
1YTV 2005-04-12 1.8 Maltose-binding protein fusion to a C-terminal fragment of the V1a vasopressin receptor
1DMB 1993-10-31 1.8 REFINED 1.8 ANGSTROMS STRUCTURE REVEALS THE MECHANISM OF BINDING OF A CYCLIC SUGAR, BETA-CYCLODEXTRIN, TO THE MALTODEXTRIN BINDING PROTEIN
5II5 2017-06-14 1.8 Crystal structure of red abalone VERL repeat 1 at 1.8 A resolution
3N94 2011-06-08 1.8 Crystal structure of human pituitary adenylate cyclase 1 Receptor-short N-terminal extracellular domain
5CFV 2016-07-13 1.8 Fusion of Maltose-binding Protein and PilA from Acinetobacter nosocomialis M2
6QGD 2019-06-12 1.8 Structure of human Mcl-1 in complex with thienopyrimidine inhibitor
3CSG 2008-07-22 1.8 Crystal Structure of Monobody YS1(MBP-74)/Maltose Binding Protein Fusion Complex
5ZCA 2018-08-15 1.8 Crystal structure of lambda repressor (1-20) fused with maltose-binding protein
1OMP 1994-01-31 1.8 CRYSTALLOGRAPHIC EVIDENCE OF A LARGE LIGAND-INDUCED HINGE-TWIST MOTION BETWEEN THE TWO DOMAINS OF THE MALTODEXTRIN-BINDING PROTEIN INVOLVED IN ACTIVE TRANSPORT AND CHEMOTAXIS
5AZ9 2016-01-13 1.82 Crystal structure of (5-residue deleted)MBP-Tom20 fusion protein tethered with ALDH presequence via a disulfide bond
5H7N 2017-02-15 1.85 Crystal structure of human NLRP12-PYD with a MBP tag
3SEU 2011-09-21 1.85 Zn-mediated Polymer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form III)
1LAX 2003-03-04 1.85 CRYSTAL STRUCTURE OF MALE31, A DEFECTIVE FOLDING MUTANT OF MALTOSE-BINDING PROTEIN
3RUM 2012-06-06 1.85 New strategy to analyze structures of glycopeptide antibiotic-target complexes
4WGI 2014-11-19 1.85 A Single Diastereomer of a Macrolactam Core Binds Specifically to Myeloid Cell Leukemia 1 (MCL1)
3SEY 2011-09-21 1.85 Zn-mediated Polymer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form II)
3G7V 2009-06-23 1.86 Islet Amyloid Polypeptide (IAPP or Amylin) fused to Maltose Binding Protein
5AQ9 2016-03-23 1.86 DARPin-based Crystallization Chaperones exploit Molecular Geometry as a Screening Dimension in Protein Crystallography
1MDQ 1994-11-01 1.9 REFINED STRUCTURES OF TWO INSERTION(SLASH)DELETION MUTANTS PROBE FUNCTION OF THE MALTODEXTRIN BINDING PROTEIN
1FQA 2001-03-14 1.9 STRUCTURE OF MALTOTETRAITOL BOUND TO OPEN-FORM MALTODEXTRIN BINDING PROTEIN IN P2(1)CRYSTAL FORM
1EZ9 2002-01-30 1.9 STRUCTURE OF MALTOTETRAITOL BOUND TO OPEN-FORM MALTODEXTRIN BINDING PROTEIN IN P1 CRYSTAL FORM
1FQB 2001-03-14 1.9 STRUCTURE OF MALTOTRIOTOL BOUND TO OPEN-FORM MALTODEXTRIN BINDING PROTEIN IN P2(1)CRYSTAL FORM
3SES 2011-09-21 1.9 Cu-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization
4GLI 2012-10-17 1.9 Crystal Structure of Human SMN YG-Dimer
4WMW 2015-05-06 1.9 The structure of MBP-MCL1 bound to ligand 5 at 1.9A
1JVY 2001-09-12 1.9 Maltodextrin-binding protein variant D207C/A301GS/P316C with beta-mercaptoethanol mixed disulfides
3Q25 2011-06-01 1.9 Crystal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP)
4WVI 2015-09-23 1.9 Crystal structure of the Type-I signal peptidase from Staphylococcus aureus (SpsB) in complex with a substrate peptide (pep2).
3WAI 2013-07-17 1.9 Crystal structure of the C-terminal globular domain of oligosaccharyltransferase (AfAglB-L, O29867_ARCFU) from Archaeoglobus fulgidus as a MBP fusion
5M15 2017-11-15 1.9 Synthetic nanobody in complex with MBP
3SET 2011-09-21 1.9 Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)
4TSM 2015-01-14 1.9 MBP-fusion protein of PilA1 from C. difficile R20291 residues 26-166
1Y4C 2005-02-15 1.9 Designed Helical Protein fusion MBP
3OSQ 2011-10-26 1.9 Maltose-bound maltose sensor engineered by insertion of circularly permuted green fluorescent protein into E. coli maltose binding protein at position 175
5B3Y 2016-10-26 1.9 Crystal structure of hPin1 WW domain (5-23) fused with maltose-binding protein
4WMS 2015-05-06 1.9 STRUCTURE OF APO MBP-MCL1 AT 1.9A
3MP8 2011-05-04 1.92 Crystal structure of Sgf29 tudor domain
3H3G 2009-08-11 1.94 Crystal structure of the extracellular domain of the human parathyroid hormone receptor (PTH1R) in complex with parathyroid hormone-related protein (PTHrP)
5C7R 2015-09-30 1.94 Revealing surface waters on an antifreeze protein by fusion protein crystallography
3C4M 2008-04-08 1.95 Structure of human parathyroid hormone in complex with the extracellular domain of its G-protein-coupled receptor (PTH1R)
5BJZ 2018-01-17 1.95 Crystal structure of maltose binding protein in complex with an allosteric synthetic antibody
3SEX 2011-09-21 1.95 Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form II)
2XZ3 2011-03-02 1.95 BLV TM hairpin
4KYC 2013-09-25 1.95 Structure of the C-terminal domain of the Menangle virus phosphoprotein, fused to MBP.
4WVJ 2015-09-23 1.95 Crystal structure of the Type-I signal peptidase from Staphylococcus aureus (SpsB) in complex with an inhibitor peptide (pep3).
5T0A 2017-02-01 1.95 Crystal Structure of Heparan Sulfate 6-O-Sulfotransferase with bound PAP and heptasaccharide substrate
5T05 2017-02-01 1.95 Crystal structure of heparan sulfate 6-O-sulfotransferase with bound PAP and IdoA2S containing hexasaccharide substrate
3EHU 2008-09-30 1.96 Crystal structure of the extracellular domain of human corticotropin releasing factor receptor type 1 (CRFR1) in complex with CRF
5GRU 2016-10-12 1.96 Structure of mono-specific diabody
5AZ7 2016-01-13 1.96 Crystal structure of MBP-Tom20 fusion protein with a 4-residue spacer in the connector helix
4XA2 2016-01-20 1.98 Structure of the Major Type IV pilin of Acinetobacter baumannii
4EGC 2013-02-27 1.99 Crystal Structure of MBP-fused Human Six1 Bound to Human Eya2 Eya Domain
4IFP 2013-04-03 1.99 X-ray Crystal Structure of Human NLRP1 CARD Domain
1JW4 2002-01-30 2.0 Structure of ligand-free maltodextrin-binding protein
1ZJL 2005-12-20 2.0 Crystal structure of zinc-bound engineered maltose binding protein
3CSB 2008-07-22 2.0 Crystal Structure of Monobody YSX1/Maltose Binding Protein Fusion Complex
4YS9 2016-03-09 2.0 Ataxin-3 Carboxy-Terminal Region - Crystal C1 (tetragonal)
1JW5 2002-01-30 2.0 Structure of Maltose Bound to Open-form Maltodextrin-binding Protein in P1 Crystal
5TTD 2017-03-01 2.0 Minor pilin FctB from S. pyogenes with engineered intramolecular isopeptide bond
3OSR 2011-10-26 2.0 Maltose-bound maltose sensor engineered by insertion of circularly permuted green fluorescent protein into E. coli maltose binding protein at position 311
5BMY 2016-10-26 2.0 Crystal structure of hPin1 WW domain (5-21) fused with maltose-binding protein
1MPB 1995-10-15 2.0 MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG)
4WMX 2015-05-06 2.0 The structure of MBP-MCL1 bound to ligand 6 at 2.0A
5II4 2017-06-14 2.0 Crystal structure of red abalone VERL repeat 1 with linker at 2.0 A resolution
5GPP 2017-08-09 2.0 Crystal structure of zebrafish ASC PYD domain
3HPI 2010-02-09 2.0 Crystal structure of maltose-binding protein mutant with bound sucrose
1ZIU 2005-12-20 2.0 Crystal Structure of nickel-bound engineered Maltose Binding Protein
5WPZ 2017-02-15 2.0 Crystal structure of MNDA PYD with MBP tag
2OK2 2007-04-10 2.0 MutS C-terminal domain fused to Maltose Binding Protein
3WOA 2015-04-29 2.0 Crystal structure of lambda repressor (1-45) fused with maltose-binding protein
3LC8 2011-02-16 2.0 Crystal structure of the cytoplasmic tail of (pro)renin receptor as a MBP fusion (Maltose-free form)
4R0Y 2014-09-10 2.0 Structure of Maltose-binding Protein Fusion with the C-terminal GH1 domain of Guanylate Kinase-associated Protein from Rattus norvegicus
5JON 2016-11-30 2.04 Crystal structure of the unliganded form of HCN2 CNBD
4WVG 2015-09-23 2.05 Crystal structure of the Type-I signal peptidase from Staphylococcus aureus (SpsB).
3VFJ 2013-01-09 2.05 The structure of monodechloro-teicoplanin in complex with its ligand, using MBP as a ligand carrier
5OSQ 2017-09-06 2.05 ZP-N domain of mammalian sperm receptor ZP3 (crystal form II, processed in P21221)
6D1U 2018-09-05 2.05 Crystal structure of the human CLR:RAMP1 extracellular domain heterodimer in complex with adrenomedullin 2/intermedin
5Z0R 2018-04-11 2.05 Structural insight into the Zika virus capsid encapsulating the viral genome
3VD8 2013-01-16 2.07 Crystal structure of human AIM2 PYD domain with MBP fusion
5AZA 2016-01-13 2.08 Crystal structure of MBP-sAglB fusion protein with a 20-residue spacer in the connector helix
5YGP 2018-10-03 2.09 Human TNFRSF25 death domain mutant-D412E
3PGF 2011-03-09 2.1 Crystal structure of maltose bound MBP with a conformationally specific synthetic antigen binder (sAB)
5GPQ 2017-08-09 2.1 Crystal Structure of zebrafish ASC CARD Domain
5FIO 2016-11-16 2.1 DARPins as a new tool for experimental phasing in protein crystallography
5GXV 2017-07-19 2.1 Crystal structure of PigG
4WVH 2015-09-23 2.1 Crystal structure of the Type-I signal peptidase from Staphylococcus aureus (SpsB) in complex with a substrate peptide (pep1).
3OAI 2011-12-21 2.1 Crystal structure of the extra-cellular domain of human myelin protein zero
5T03 2017-02-01 2.1 Crystal structure of heparan sulfate 6-O-sulfotransferase with bound PAP and glucuronic acid containing hexasaccharide substrate
2VGQ 2007-12-11 2.1 Crystal Structure of Human IPS-1 CARD
1MH3 2002-09-18 2.1 maltose binding-a1 homeodomain protein chimera, crystal form I
1MPC 1995-10-15 2.1 MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG)
1NL5 2003-08-12 2.1 Engineered High-affinity Maltose-Binding Protein
5K94 2017-06-07 2.1 Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase
4XZS 2016-01-20 2.12 Crystal Structure of TRIAP1-MBP fusion
5E24 2016-06-15 2.14 Structure of the Su(H)-Hairless-DNA Repressor Complex
4H1G 2012-10-10 2.15 Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein
3LBS 2011-02-16 2.15 Crystal structure of the cytoplasmic tail of (pro)renin receptor as a MBP fusion (Maltose-bound form)
5BK1 2018-01-17 2.15 Crystal structure of maltose binding protein in complex with an endosteric synthetic antibody
5ZD4 2018-08-29 2.17 Crystal structure of MBP-fused BIL1/BZR1 in complex with double-stranded DNA
5JST 2017-03-22 2.2 MBP fused MDV1 coiled coil
5IHJ 2016-09-21 2.2 Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii BIDMC57
5LOF 2016-10-26 2.2 Crystal structure of the MBP-MCL1 complex with highly selective and potent inhibitor of MCL1
4KV3 2014-04-09 2.2 Ubiquitin-like domain of the Mycobacterium tuberculosis type VII secretion system protein EccD1 as maltose-binding protein fusion
3RLF 2011-08-10 2.2 Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to MgAMPPNP
1ZKB 2005-12-20 2.2 Zinc-free Engineered maltose binding protein
4KYD 2013-09-25 2.21 Partial Structure of the C-terminal domain of the HPIV4B phosphoprotein, fused to MBP.
4OGM 2015-01-14 2.23 MBP-fusion protein of PilA1 residues 26-159
6D66 2018-09-19 2.23 Crystal structure of the human dual specificity 1 catalytic domain (C258S) as a maltose binding protein fusion in complex with the designed AR protein mbp3_16
1SVX 2004-05-25 2.24 Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein
5GXT 2017-07-19 2.24 Crystal structure of PigG
3HST 2010-04-28 2.25 N-Terminal RNASE H domain of rv2228c from mycobacterium tuberculosis as a fusion protein with maltose binding protein
4XR8 2016-02-03 2.25 Crystal structure of the HPV16 E6/E6AP/p53 ternary complex at 2.25 A resolution
4WTH 2016-03-09 2.25 Ataxin-3 Carboxy Terminal Region - Crystal C2 (triclinic)
4JKM 2014-09-17 2.26 Crystal Structure of Clostridium perfringens beta-glucuronidase
6ANV 2017-10-25 2.27 Crystal structure of anti-CRISPR protein AcrF1
6NDJ 2019-01-23 2.27 Crystal structure of human NLRP6 PYD domain with MBP fusion
4DXB 2012-08-08 2.29 2.29A structure of the engineered MBP TEM-1 fusion protein RG13 in complex with zinc, P1 space group
3PY7 2011-12-14 2.29 Crystal structure of full-length Bovine Papillomavirus oncoprotein E6 in complex with LD1 motif of paxillin at 2.3A resolution
6EQZ 2017-11-01 2.29 A MamC-MIC insertion in MBP scaffold at position K170
1FQD 2001-03-14 2.3 CRYSTAL STRUCTURE OF MALTOTETRAITOL BOUND TO CLOSED-FORM MALTODEXTRIN BINDING PROTEIN
6AEO 2019-06-12 2.3 TssL periplasmic domain
1MH4 2002-09-18 2.3 maltose binding-a1 homeodomain protein chimera, crystal form II
4DXC 2012-08-08 2.3 Crystal structure of the engineered MBP TEM-1 fusion protein RG13, C2 space group
3PUW 2011-08-10 2.3 Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to ADP-AlF4
1HSJ 2001-06-06 2.3 SARR MBP FUSION STRUCTURE
1MPD 1995-10-15 2.3 MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG), COMPLEXED WITH MALTOSE
3Q29 2011-06-01 2.3 Cyrstal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP)
5DFM 2015-10-28 2.3 Structure of Tetrahymena telomerase p19 fused to MBP
5B3Z 2016-10-26 2.3 Crystal structure of hPin1 WW domain (5-39) fused with maltose-binding protein
1MDP 1994-11-01 2.3 REFINED STRUCTURES OF TWO INSERTION(SLASH)DELETION MUTANTS PROBE FUNCTION OF THE MALTODEXTRIN BINDING PROTEIN
3D4G 2008-12-02 2.3 ZP-N domain of mammalian sperm receptor ZP3 (crystal form II)
1FQC 2001-03-14 2.3 CRYSTAL STRUCTURE OF MALTOTRIOTOL BOUND TO CLOSED-FORM MALTODEXTRIN BINDING PROTEIN
5CBN 2016-03-30 2.3 Fusion protein of mbp3-16 and B4 domain of protein A from staphylococcal aureus with chemical cross-linker EY-CBS
3PUX 2011-08-10 2.3 Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to ADP-BeF3
1NMU 2003-02-18 2.31 MBP-L30
5IQZ 2016-07-27 2.33 Crystal structure of N-terminal domain of Human SIRT7
6D65 2018-09-19 2.35 Crystal structure of the human dual specificity phosphatase 1 catalytic domain (C258S) as a maltose binding protein fusion in complex with the designed AR protein off7
3SER 2011-09-21 2.35 Zn-mediated Polymer of Maltose-binding Protein K26H/K30H by Synthetic Symmetrization
4WMT 2015-05-06 2.35 STRUCTURE OF MBP-MCL1 BOUND TO ligand 1 AT 2.35A
2OBG 2007-03-27 2.35 Crystal Structure of Monobody MBP-74/Maltose Binding Protein Fusion Complex
3H4Z 2010-03-31 2.35 Crystal Structure of an MBP-Der p 7 fusion protein
4KI0 2013-10-23 2.38 Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose
3PUV 2011-08-10 2.4 Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to ADP-VO4
5B3X 2016-10-26 2.4 Crystal structure of hPin1 WW domain (5-15) fused with maltose-binding protein in P41212 form
5B3W 2016-10-26 2.4 Crystal structure of hPin1 WW domain (5-15) fused with maltose-binding protein in C2221 form
4MY2 2013-11-13 2.4 Crystal Structure of Norrin in fusion with Maltose Binding Protein
3DM0 2008-09-09 2.4 Maltose Binding Protein fusion with RACK1 from A. thaliana
4WMV 2015-05-06 2.4 STRUCTURE OF MBP-MCL1 BOUND TO ligand 4 AT 2.4A
5W0R 2017-08-16 2.4 Crystal structure of MBP fused activation-induced cytidine deaminase (AID) in complex with cacodylic acid
4JBZ 2013-12-11 2.4 Structure of Mcm10 coiled-coil region
5I04 2017-06-07 2.42 Crystal structure of the orphan region of human endoglin/CD105
4RWG 2015-05-20 2.44 Crystal structure of the CLR:RAMP1 extracellular domain heterodimer with bound high affinity CGRP analog
4IKM 2013-05-08 2.46 X-ray structure of CARD8 CARD domain
6APX 2017-11-01 2.49 Crystal structure of human dual specificity phosphatase 1 catalytic domain (C258S) as a maltose binding protein fusion in complex with the monobody YSX1
4N4X 2014-09-17 2.5 Crystal Structure of the MBP fused human SPLUNC1 (native form)
1N3X 2003-08-12 2.5 Ligand-free High-Affinity Maltose-Binding Protein
5YEV 2018-09-19 2.5 Murine DR3 death domain
6N85 2019-07-10 2.5 Resistance to inhibitors of cholinesterase 8A (Ric8A) protein in complex with MBP-tagged transducin-alpha residues 327-350
1MG1 1999-04-06 2.5 HTLV-1 GP21 ECTODOMAIN/MALTOSE-BINDING PROTEIN CHIMERA
4KEG 2014-04-30 2.5 Crystal Structure of MBP Fused Human SPLUNC1
3A3C 2009-08-04 2.5 Crystal structure of TIM40/MIA40 fusing MBP, C296S and C298S mutant
1ZMG 2005-12-20 2.5 Crystal structure of copper-bound engineered maltose binding protein
1JVX 2001-09-12 2.5 Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal
6D67 2018-09-19 2.55 Crystal structure of the human dual specificity phosphatase 1 catalytic domain (C258S) as a maltose binding protein fusion (maltose bound form) in complex with the designed AR protein mbp3_16
5ZNZ 2019-04-17 2.55 Structure of mDR3 DD with MBP tag mutant-I387V
4GIZ 2013-01-23 2.55 Crystal structure of full-length human papillomavirus oncoprotein E6 in complex with LXXLL peptide of ubiquitin ligase E6AP at 2.55 A resolution
5AZ6 2016-01-13 2.56 Crystal structure of MBP-Tom20 fusion protein with a 2-residue spacer in the connector helix
1PEB 2003-08-12 2.6 LIGAND-FREE HIGH-AFFINITY MALTOSE-BINDING PROTEIN
3MP1 2011-05-04 2.6 Complex structure of Sgf29 and trimethylated H3K4
4XAI 2015-03-04 2.6 Crystal Structure of red flour beetle NR2E1/TLX
4KYE 2013-09-25 2.6 Partial Structure of the C-terminal domain of the HPIV4B phosphoprotein, fused to MBP.
5BK2 2018-01-17 2.6 Crystal structure of maltose binding protein in complex with a peristeric synthetic antibody
1N3W 2003-08-12 2.6 Engineered High-Affinity Maltose-Binding Protein
3F5F 2008-12-16 2.65 Crystal structure of heparan sulfate 2-O-sulfotransferase from gallus gallus as a maltose binding protein fusion.
5YGS 2018-10-03 2.69 Human TNFRSF25 death domain
4LOG 2013-10-09 2.7 The crystal structure of the orphan nuclear receptor PNR ligand binding domain fused with MBP
5I69 2016-03-23 2.7 MBP-MamC magnetite-interaction component mutant-D70A
5HZV 2017-06-07 2.7 Crystal structure of the zona pellucida module of human endoglin/CD105
6DM8 2018-08-01 2.7 Understanding the Species Selectivity of Myeloid cell leukemia-1 (Mcl-1) inhibitors
4O2X 2014-12-24 2.7 Structure of a malarial protein
1IUD 1997-06-05 2.7 MALTODEXTRIN-BINDING PROTEIN INSERTION/DELETION MUTANT WITH AN INSERTED B-CELL EPITOPE FROM THE PRES2 REGION OF HEPATITIS B VIRUS
3OB4 2011-02-02 2.71 MBP-fusion protein of the major peanut allergen Ara h 2
5ZNY 2019-04-17 2.74 Structure of mDR3_DD-C363G with MBP tag
3EHS 2008-09-30 2.76 Crystal structure of the extracellular domain of human corticotropin releasing factor receptor type 1 (CRFR1)
6DKS 2019-01-02 2.78 Structure of the Rbpj-SHARP-DNA Repressor Complex
5EDU 2016-07-27 2.79 Crystal structure of human histone deacetylase 6 catalytic domain 2 in complex with trichostatin A
4BLD 2013-11-27 2.8 Crystal structure of a human Suppressor of fused (SUFU)-GLI3p complex
4BL9 2013-11-27 2.8 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I)
1R6Z 2004-01-13 2.8 The Crystal Structure of the Argonaute2 PAZ domain (as a MBP fusion)
4FEB 2013-03-13 2.8 Crystal Structure of Htt36Q3H-EX1-X1-C2(Beta)
6CXS 2019-04-17 2.8 Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine
2R6G 2007-11-27 2.8 The Crystal Structure of the E. coli Maltose Transporter
5V6Y 2018-01-31 2.8 Crystal structure of the human CLR:RAMP1 extracellular domain heterodimer with bound high-affinity and altered selectivity adrenomedullin variant
5E7U 2016-03-23 2.8 MBP-MamC loop structure, a magnetite biomineralizing protein from Magnetospirillium magneticum AMB-1
5WVN 2018-01-03 2.8 Crystal structure of MBS-BaeS fusion protein
4BLB 2013-11-27 2.8 Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex
4NUF 2014-01-29 2.8 Crystal Structure of SHP/EID1
3MQ9 2010-10-27 2.8 Crystal Structure of Ectodomain Mutant of BST-2/Tetherin/CD317 Fused to MBP
4FED 2013-03-13 2.81 Crystal Structure of Htt36Q3H
5JJ4 2016-06-29 2.81 Crystal Structure of a Variant Human Activation-induced Deoxycytidine Deaminase as an MBP fusion protein
6EG3 2018-10-31 2.84 Crystal structure of human BRM in complex with compound 15
6DD5 2018-10-17 2.85 Crystal Structure of the Cas6 Domain of Marinomonas mediterranea MMB-1 Cas6-RT-Cas1 Fusion Protein
5DIS 2015-11-04 2.85 Crystal structure of a CRM1-RanGTP-SPN1 export complex bound to a 113 amino acid FG-repeat containing fragment of Nup214
4QSZ 2015-07-08 2.86 Crystal structure of mouse JMJd7 fused with maltose-binding protein
1A7L 1998-06-17 2.9 DOMINANT B-CELL EPITOPE FROM THE PRES2 REGION OF HEPATITIS B VIRUS IN THE FORM OF AN INSERTED PEPTIDE SEGMENT IN MALTODEXTRIN-BINDING PROTEIN
3PUZ 2011-05-18 2.9 Crystal Structure of a pre-translocation state MBP-Maltose transporter complex bound to AMP-PNP
5W0U 2017-08-16 2.9 Crystal structure of MBP fused activation-induced cytidine deaminase (AID) in complex with dCMP
5IIC 2017-06-14 2.9 Crystal structure of red abalone VERL repeat 3 at 2.9 A resolution
5WVM 2018-01-03 2.9 Crystal structure of baeS cocrystallized with 2 mM indole
4KHZ 2013-10-23 2.9 Crystal structure of the maltose-binding protein/maltose transporter complex in an pre-translocation conformation bound to maltoheptaose
3D4C 2008-12-02 2.9 ZP-N domain of mammalian sperm receptor ZP3 (crystal form I)
6I4Y 2019-03-20 2.91 X-ray structure of the human mitochondrial PRELID3b-TRIAP1 complex
5Z0V 2018-04-11 2.91 Structural insight into the Zika virus capsid encapsulating the viral genome
5LEM 2017-08-02 2.98 Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_11_3G124 in complex with Maltose-binding Protein and Green Fluorescent Protein
6EG2 2018-10-31 2.98 Crystal structure of human BRM in complex with compound 16
2ZXT 2009-08-04 3.0 Crystal structure of Tim40/MIA40, a disulfide relay system in mitochondria, solved as MBP fusion protein
6DBJ 2018-08-01 3.0 Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates
5Y2G 2019-02-27 3.0 Structure of MBP tagged GBS CAMP
4FEC 2013-03-13 3.0 Crystal Structure of Htt36Q3H
4FE8 2013-03-13 3.0 Crystal Structure of Htt36Q3H-EX1-X1-C1(Alpha)
4BL8 2013-11-27 3.04 Crystal structure of full-length human Suppressor of fused (SUFU)
3SEV 2011-09-21 3.05 Zn-mediated Trimer of Maltose-binding Protein E310H/K314H by Synthetic Symmetrization
3EF7 2008-12-02 3.1 ZP-N domain of mammalian sperm receptor ZP3 (crystal form III)
3PV0 2011-05-18 3.1 Crystal Structure of a pre-translocation state MBP-Maltose transporter complex without nucleotide
5LEL 2017-11-15 3.1 Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_10_3G124 in complex with Maltose-binding Protein and Green Fluorescent Protein
3PUY 2011-05-18 3.1 Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to AMP-PNP after crystal soaking of the pretranslocation state
5JQE 2016-10-26 3.16 Crystal structure of caspase8 tDED
5W1C 2017-08-16 3.18 Crystal structure of MBP fused activation-induced cytidine deaminase (AID) in complex with cytidine
4WRN 2016-01-27 3.2 Crystal structure of the polymerization region of human uromodulin/Tamm-Horsfall protein
4WJV 2014-11-19 3.2 Crystal structure of Rsa4 in complex with the Nsa2 binding peptide
5FSG 2016-04-06 3.21 Structure of the hantavirus nucleoprotein provides insights into the mechanism of RNA encapsidation and a template for drug design
3L2J 2010-02-02 3.24 Dimeric structure of the ligand-free extracellular domain of the human parathyroid hormone receptor (PTH1R)
1T0K 2004-07-20 3.24 Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex
4B3N 2012-10-24 3.3 Crystal structure of rhesus TRIM5alpha PRY/SPRY domain
6DBI 2018-08-01 3.4 Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates
4PQK 2014-06-18 3.4 C-Terminal domain of DNA binding protein
3EHT 2008-09-30 3.4 Crystal structure of the extracellular domain of human corticotropin releasing factor receptor type 1 (CRFR1) in complex with CRF
4NDZ 2014-03-19 3.45 Structure of Maltose Binding Protein fusion to 2-O-Sulfotransferase with bound heptasaccharide and PAP
3IOT 2009-09-29 3.5 Huntingtin amino-terminal region with 17 Gln residues - crystal C92-b
4BLA 2013-11-27 3.5 Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II)
3IOW 2009-09-29 3.5 Huntingtin amino-terminal region with 17 Gln residues - crystal C99-Hg
4XAJ 2015-03-04 3.55 Crystal structure of human NR2E1/TLX
4XZV 2016-01-20 3.58 Crystal Structure of SLMO1-TRIAP1 Complex
5GS2 2016-10-12 3.59 Crystal structure of diabody complex with repebody and MBP
3IOR 2009-09-29 3.6 Huntingtin amino-terminal region with 17 Gln residues - crystal C95
5W0Z 2017-08-16 3.61 Crystal structure of MBP fused activation-induced cytidine deaminase (AID)
3IO4 2009-10-27 3.63 Huntingtin amino-terminal region with 17 Gln residues - Crystal C90
3IO6 2009-10-27 3.7 Huntingtin amino-terminal region with 17 Gln residues - crystal C92-a
3IOV 2009-09-29 3.7 Huntingtin amino-terminal region with 17 Gln residues - crystal C99
3IOU 2009-09-29 3.7 Huntingtin amino-terminal region with 17 Gln residues - crystal C94
5CL1 2015-08-12 3.8 Complex structure of Norrin with human Frizzled 4
6DBU 2018-08-01 3.9 Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
6BUZ 2017-12-20 3.92 Cryo-EM structure of CENP-A nucleosome in complex with kinetochore protein CENP-N
6DBR 2018-08-01 4.0 Cryo-EM structure of RAG in complex with one melted RSS and one unmelted RSS
6DBX 2018-08-01 4.2 Cryo-EM structure of RAG in complex with 12-RSS substrate DNA
6DBQ 2018-08-01 4.22 Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
3J9P 2015-04-08 4.24 Structure of the TRPA1 ion channel determined by electron cryo-microscopy
6DBV 2018-08-01 4.29 Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
6DBT 2018-08-01 4.3 Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
6DBO 2018-08-01 4.4 Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
5HZW 2017-06-07 4.45 Crystal structure of the orphan region of human endoglin/CD105 in complex with BMP9
6DBW 2018-08-01 4.7 Cryo-EM structure of RAG in complex with 12-RSS substrate DNA
6DBL 2018-08-01 5.0 Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
5LDF 2016-08-10 6.2 Maltose binding protein genetically fused to dodecameric glutamine synthetase

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
90.7 Maltose-binding periplasmic protein P41130 MALE_PHOLU
93.9 Maltose-binding periplasmic protein P18815 MALE_KLEAE
94.3 Maltose-binding periplasmic protein P19576 MALE_SALTY
100.0 Maltose-binding periplasmic protein P0AEX9 MALE_ECOLI
100.0 Maltose-binding periplasmic protein P0AEY0 MALE_ECO57