Preferential assembly of the tropomyosin heterodimer: equilibrium studies.


Abstract

Thermal unfolding/refolding studies of the three tropomyosin dimers, alpha alpha, alpha beta, and beta beta, from chicken gizzard muscle were performed to explain the preferential assembly of alpha- and beta-tropomyosin subunits into heterodimers, alpha beta [Lehrer, S. S., & Qian, Y. (1989) J. Biol. Chem. 265, 1134]. Circular dichroism measurements showed that all three dimers unfolded in cooperative reversible transitions with T1/2 = 40.0 degrees C and delta H degrees = 162 kcal/mol for alpha alpha and with T1/2 = 42.6 degrees C and delta H degree = 98 kcal/mol for beta beta at 0.4-0.5 microM concentrations. Fluorescence measurements on pyrenyliodoacetamide-labeled tropomyosin showed that (i) excimer fluorescence decreases in parallel with unfolding of homodimers, (ii) at physiological temperature, heterodimers are formed from micromolar mixtures of homodimers over a period of minutes, and (iii) heterodimers unfold/refold with temperature without appreciable formation of homodimers. To understand the preferential formation of alpha beta, we calculated the concentrations of all species present as a function of temperature for equal total amounts of alpha and beta, using the measured thermodynamic constants of the unfolding/dissociation equilibria for alpha alpha and beta beta. Values for delta H degrees = 225 kcal/mol and T1/2 = 43 degrees C for unfolding of alpha beta at 0.5 microM concentration were obtained from the best fit of the calculations to the measured helical content vs temperature of alpha beta.(ABSTRACT TRUNCATED AT 250 WORDS) Study holds ProTherm entries: 4366, 4367, 4368, 4369, 4370, 4371 Extra Details: additive : EDTA(1 mM),

Submission Details

ID: r4t7dUvK3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:25 p.m.

Version: 1

Publication Details
Lehrer SS;Stafford WF,Biochemistry (1991) Preferential assembly of the tropomyosin heterodimer: equilibrium studies. PMID:2043610
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1MV4 2003-02-18 TM9A251-284: A Peptide Model of the C-Terminus of a Rat Striated Alpha Tropomyosin
2W4U 2010-08-25 Isometrically contracting insect asynchronous flight muscle quick frozen after a length step
2G9J 2006-11-07 Complex of TM1a(1-14)Zip with TM9a(251-284): a model for the polymerization domain ('overlap region') of tropomyosin, Northeast Structural Genomics Target OR9
2W49 2010-05-05 ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE
5KHT 2017-06-21 1.5 Crystal structure of the N-terminal fragment of tropomyosin isoform Tpm1.1 at 1.5 A resolution
3U1C 2011-11-23 1.8 Anti-parallel dimer of N-terminal 98-aa fragment of smooth muscle tropomyosin alpha
2EFR 2008-03-04 1.8 Crystal structure of the c-terminal tropomyosin fragment with N- and C-terminal extensions of the leucine zipper at 1.8 angstroms resolution
2TMA 1987-10-16 15.0 TROPOMYOSIN CRYSTAL STRUCTURE AND MUSCLE REGULATION. APPENDIX. CONSTRUCTION OF AN ATOMIC MODEL FOR TROPOMYOSIN AND IMPLICATIONS FOR INTERACTIONS WITH ACTIN
3U1A 2011-11-23 2.0 N-terminal 81-aa fragment of smooth muscle tropomyosin alpha
2EFS 2008-03-04 2.0 Crystal structure of the C-terminal tropomyosin fragment with N- and C-terminal extensions of the leucine zipper at 2.0 angstroms resolution
1IC2 2001-07-25 2.0 DECIPHERING THE DESIGN OF THE TROPOMYOSIN MOLECULE
2Z5I 2008-04-22 2.1 Crystal structure of the head-to-tail junction of tropomyosin
3MTU 2010-06-23 2.1 Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle
3MUD 2010-06-23 2.2 Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle
2B9C 2006-01-03 2.3 Structure of tropomyosin's mid-region: bending and binding sites for actin
6OTN 2019-08-14 2.4 Crystal Structure of an N-terminal Fragment of Cancer Associated Tropomyosin 3.1 (Tpm3.1)
2D3E 2006-09-12 2.6 Crystal structure of the C-Terminal fragment of rabbit skeletal alpha-tropomyosin
1KQL 2002-05-29 2.7 Crystal structure of the C-terminal region of striated muscle alpha-tropomyosin at 2.7 angstrom resolution
2Z5H 2008-04-22 2.89 Crystal structure of the head-to-tail junction of tropomyosin complexed with a fragment of TnT
1C1G 2000-02-11 7.0 CRYSTAL STRUCTURE OF TROPOMYOSIN AT 7 ANGSTROMS RESOLUTION IN THE SPERMINE-INDUCED CRYSTAL FORM
4A7F 2012-08-01 7.7 Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 3)
4A7H 2012-08-01 7.8 Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2)
4A7L 2012-08-01 8.1 Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 1)

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Tropomyosin alpha-1 chain Q01173 TPM1_XENLA
100.0 Tropomyosin alpha-1 chain P04692 TPM1_RAT
100.0 Tropomyosin alpha-1 chain P13105 TPM1_RANTE
100.0 Tropomyosin alpha-1 chain P58772 TPM1_RABIT
100.0 Tropomyosin alpha-1 chain P42639 TPM1_PIG
100.0 Tropomyosin alpha-1 chain P58771 TPM1_MOUSE
100.0 Tropomyosin alpha-1 chain P09493 TPM1_HUMAN
100.0 Tropomyosin alpha-1 chain P13104 TPM1_DANRE
100.0 Tropomyosin alpha-1 chain P58773 TPM1_COTJA
100.0 Tropomyosin alpha-1 chain P04268 TPM1_CHICK
100.0 Tropomyosin alpha-1 chain P84335 TPM1_CHEAU
100.0 Tropomyosin alpha-1 chain Q5KR49 TPM1_BOVIN
100.0 Tropomyosin alpha-1 chain A1XQV4 TPM3_PIG
100.0 Tropomyosin alpha-1 chain P06753 TPM3_HUMAN
100.0 Tropomyosin alpha-1 chain Q5KR47 TPM3_BOVIN