Effect of heme binding on the structure and stability of Escherichia coli apocytochrome b562.


Abstract

The structure and stability of apocytochrome b562 were explored using absorption and circular dichroism spectroscopic methods. The polypeptide chain retains a well-defined structure when the prosthetic heme group is removed from cytochrome b562. Circular dichroism measurements estimate 60% helicity for apocytochrome b562, compared with 80% helicity found in holocytochrome b562. At low pH, apocytochrome b562 displays a midpoint pH of 2.9, while ferricytochrome b562 displays a midpoint pH of 2.3. The unfolding of the apoprotein by urea and heat can be well approximated by the two-state transition model. The stability of apocytochrome b562 is significantly reduced from that of the holoprotein. The free energy of stabilization (delta G degrees) and the midpoint transition temperature (Tm) for apocytochrome b562 are found to be 3.2 +/- 0.5 kcal/mol and 52.3 +/- 0.9 degrees C, respectively, compared with 6.6 +/- 0.5 kcal/mol and 67.2 +/- 0.5 degrees C for ferricytochrome b562. The smaller heat capacity change upon unfolding of apocytochrome b562 than that of ferricytochrome b562, estimated from the thermodynamic parameters, indicates that apocytochrome b562 possesses a smaller hydrophobic core than holocytochrome b562. Size-exclusion chromatography studies indicate that the apoprotein is slightly more extended in molecular dimension than ferricytochrome b562. The data suggest that apocytochrome b562 resembles a "molten globule" or a "collapsed form" of the holoprotein, in which secondary structure formation is largely complete while the global folding is either only partially complete or dynamically expanded. Study holds ProTherm entries: 3958, 3959, 3960, 3961 Extra Details: hydrophobic core; molten globule;collapsed form;,partially complete

Submission Details

ID: qjQXMLhk

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:23 p.m.

Version: 1

Publication Details
Feng YQ;Sligar SG,Biochemistry (1991) Effect of heme binding on the structure and stability of Escherichia coli apocytochrome b562. PMID:1931945
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1QQ3 2000-05-24 THE SOLUTION STRUCTURE OF THE HEME BINDING VARIANT ARG98CYS OF OXIDIZED ESCHERICHIA COLI CYTOCHROME B562
1QPU 1999-06-02 SOLUTION STRUCTURE OF OXIDIZED ESCHERICHIA COLI CYTOCHROME B562
1APC 1994-01-31 SOLUTION STRUCTURE OF APOCYTOCHROME B562
6DYF 2019-04-24 1.1 Cu(II)-bound structure of the engineered cyt cb562 variant, CH3Y
5YO6 2018-03-14 1.2 Crystal Structure of B562RIL with engineered disulfide bond T9C-A36C
4JEA 2013-08-21 1.22 Crystal structure of an engineered Zn-RIDC1 construct with four interfacial disulfide bonds
6DYC 2019-04-24 1.33 Co(II)-bound structure of the engineered cyt cb562 variant, CH3
5YO4 2018-03-14 1.37 Crystal Structure of B562RIL with engineered disulfide bond K27C-A79C
256B 1991-01-15 1.4 IMPROVEMENT OF THE 2.5 ANGSTROMS RESOLUTION MODEL OF CYTOCHROME B562 BY REDETERMINING THE PRIMARY STRUCTURE AND USING MOLECULAR GRAPHICS
6DYG 2019-04-24 1.49 Fe(II)-bound structure of the engineered cyt cb562 variant, CH3Y
5YM7 2017-11-22 1.56 Crystal Structure of B562RIL without disulfide bond
6DYL 2019-04-24 1.69 Vanadyl-bound structure of the engineered cyt b562 variant, CH3Y*
5YO3 2018-05-09 1.7 Crystal Structure of B562RIL with engineered disulfide bond V16C-A29C
5NM4 2017-09-27 1.7 A2A Adenosine receptor room-temperature structure determined by serial femtosecond crystallography
3DE8 2009-04-21 1.72 Crystal Structure of a Dimeric Cytochrome cb562 Assembly Induced by Copper Coordination
5IU4 2016-06-29 1.72 Crystal structure of stabilized A2A adenosine receptor A2AR-StaR2-bRIL in complex with ZM241385 at 1.7A resolution
6DYD 2019-04-24 1.72 Cu(II)-bound structure of the engineered cyt cb562 variant, CH3
3C63 2008-05-06 1.75 Tetrameric Cytochrome cb562 (K34/H59/D62/H63/H73/A74/H77) Assembly Stabilized by Interprotein Zinc Coordination
6DY6 2019-04-24 1.8 Mn(II)-bound structure of the engineered cyt cb562 variant, CH2E
4N6H 2013-12-25 1.8 1.8 A Structure of the human delta opioid 7TM receptor (PSI Community Target)
4EIY 2012-07-25 1.8 Crystal structure of the chimeric protein of A2aAR-BRIL in complex with ZM241385 at 1.8A resolution
1M6T 2002-11-06 1.81 CRYSTAL STRUCTURE OF B562RIL, A REDESIGNED FOUR HELIX BUNDLE
6DYH 2019-04-24 1.83 Vanadyl-bound structure of the engineered cyt cb562 variant, CH3Y
5AWI 2015-10-21 1.85 Domain-swapped cytochrome cb562 dimer
6CBV 2019-02-06 1.87 Crystal structure of BRIL bound to an affinity matured synthetic antibody.
3C62 2008-05-06 1.87 Tetrameric Cytochrome cb562 (H59/D62/H63/H73/A74/H77) Assembly Stabilized by Interprotein Zinc Coordination
5OLG 2018-01-17 1.87 Structure of the A2A-StaR2-bRIL562-ZM241385 complex at 1.86A obtained from in meso soaking experiments.
6DY8 2019-04-24 1.9 Mn(II)-bound structure of the engineered cyt cb562 variant, CH2EY
6DY4 2019-04-24 1.9 Fe(II)-bound structure of the engineered cyt cb562 variant, CH2E
5K2C 2016-09-21 1.9 1.9 angstrom A2a adenosine receptor structure with sulfur SAD phasing and phase extension using XFEL data
5K2D 2016-09-21 1.9 1.9A angstrom A2a adenosine receptor structure with MR phasing using XFEL data
5OLZ 2018-01-17 1.9 Structure of the A2A-StaR2-bRIL562-Compound 4e complex at 1.9A obtained from bespoke co-crystallisation experiments.
5IU7 2016-06-29 1.9 Crystal structure of stabilized A2A adenosine receptor A2AR-StaR2-bRIL in complex with compound 12c at 1.9A resolution
3M4C 2011-01-12 1.9 A Zn-mediated tetrahedral protein lattice cage encapsulating a microperoxidase
5NM2 2017-09-27 1.95 A2A Adenosine receptor cryo structure
6DYK 2019-04-24 1.96 Iron- and Nitric Oxide-bound structure of the engineered cyt b562 variant, CH3Y*
5WIU 2017-10-18 1.96 Structure of the human D4 Dopamine receptor in complex with Nemonapride
6DYJ 2019-04-24 1.96 Iron-bound structure of the engineered cyt b562 variant, CH3Y*
6DYI 2019-04-24 1.96 Co(II)-bound structure of the engineered cyt cb562 variant, H3
5XZJ 2017-12-20 1.98 Crystal structure of the Zn-directed tetramer of the engineered cyt cb562 variant, C96T/AB5
3TOL 2012-07-04 2.0 Crystal structure of an engineered cytochrome cb562 that forms 1D, Zn-mediated coordination polymers
5MZJ 2017-07-26 2.0 Crystal structure of stabilized A2A adenosine receptor A2AR-StaR2-bRIL in complex with theophylline at 2.0A resolution
5OM4 2018-01-17 2.0 Structure of the A2A-StaR2-bRIL562-Compound 4e complex at 1.86A obtained from in meso soaking experiments (24 hour soak).
3HNJ 2010-02-09 2.0 Crystal structure of the Zn-induced tetramer of the engineered cyt cb562 variant RIDC-2
6GT3 2019-06-26 2.0 Crystal Structure of the A2A-StaR2-bRIL562 in complex with AZD4635 at 2.0A resolution
5OLV 2018-01-17 2.0 Structure of the A2A-StaR2-bRIL562-LUAA47070 complex at 2.0A obtained from in meso soaking experiments.
5IU8 2016-06-29 2.0 Crystal structure of stabilized A2A adenosine receptor A2AR-StaR2-bRIL in complex with compound 12f at 2.0A resolution
3NMI 2010-11-24 2.01 Crystal structure of the phenanthroline-modified cytochrome cb562 variant, MBP-Phen2
3DE9 2009-04-21 2.04 Crystal Structure of a Trimeric Cytochrome cb562 Assembly Induced by Nickel Coordination
3IQ5 2010-06-16 2.05 Crystal structure of an engineered metal-free tetrameric cytochrome cb562 complex templated by Zn-coordination
5L32 2016-11-09 2.1 Crystal structure of the Zn-RIDC1 complex bearing six interfacial disulfide bonds
5MZP 2017-07-26 2.1 Crystal structure of stabilized A2A adenosine receptor A2AR-StaR2-bRIL in complex with caffeine at 2.1A resolution
5IUB 2016-06-29 2.1 Crystal structure of stabilized A2A adenosine receptor A2AR-StaR2-bRIL in complex with compound 12x at 2.1A resolution
3M79 2010-06-16 2.1 A tetrameric Zn-bound cytochrome cb562 complex with covalently and non-covalently stabilized interfaces crystallized in the presence of Cu(II) and Zn(II)
3HNK 2010-02-09 2.1 Crystal structure of the dimeric assembly of the cyt cb562 variant RIDC-1
5OM1 2018-01-17 2.1 Structure of the A2A-StaR2-bRIL562-Compound 4e complex at 2.1A obtained from in meso soaking experiments (1 hour soak).
4JE9 2013-08-21 2.12 Crystal structure of an engineered metal-free RIDC1 construct with four interfacial disulfide bonds
5WIV 2017-10-18 2.14 Structure of the sodium-bound human D4 Dopamine receptor in complex with Nemonapride
5NLX 2017-09-27 2.14 A2A Adenosine receptor room-temperature structure determined by serial millisecond crystallography
3HNL 2010-02-09 2.2 Crystal structure of the Cu-induced dimer of the engineered cyt cb562 variant RIDC-1
5IUA 2016-06-29 2.2 Crystal structure of stabilized A2A adenosine receptor A2AR-StaR2-bRIL in complex with compound 12b at 2.2A resolution
5YO5 2018-03-14 2.2 Crystal Structure of B562RIL with engineered disulfide bond A20C-Q25C
6C1R 2018-05-30 2.2 Crystal structure of human C5a receptor in complex with an orthosteric antagonist PMX53 and an allosteric antagonist avacopan
6DHY 2018-07-25 2.22 Crystallogrpahic tetramer of Zn-bound RIDC1 variant bearing two disulfide bonded cysteines
6DYE 2019-04-24 2.25 Fe(II)-bound structure of the engineered cyt cb562 variant, CH3
2BC5 2006-09-26 2.25 Crystal structure of E. coli cytochrome b562 with engineered c-type heme linkages
4JEB 2013-08-21 2.3 Crystal structure of an engineered RIDC1 tetramer with four interfacial disulfide bonds and four three-coordinate Zn(II) sites
3TOM 2012-07-04 2.3 Crystal structure of an engineered cytochrome cb562 that forms 2D, Zn-mediated sheets
3L1M 2010-04-28 2.3 Crystal Structure of a Ni-directed Dimer of Cytochrome cb562 with a Quinolate-Histidine Hybrid Coordination Motif
5ZKC 2018-11-21 2.3 Crystal structure of rationally thermostabilized M2 muscarinic acetylcholine receptor bound with NMS
5VRA 2017-12-13 2.35 2.35-Angstrom In situ Mylar structure of human A2A adenosine receptor at 100 K
3IQ6 2010-06-16 2.35 Crystal structure of a tetrameric Zn-bound cytochrome cb562 complex with covalently and non-covalently stabilized interfaces
3HNI 2010-02-09 2.35 Crystal structure of the Zn-induced tetramer of the engineered cyt cb562 variant RIDC-1
5L31 2016-11-09 2.4 Crystal structure of an engineered metal-free RIDC1 variant containing five disulfide bonds.
3FOO 2009-07-14 2.4 A Triangular Cytochrome b562 Superstructure Mediated by Ni Coordination - Monoclinic Form
4JKV 2013-04-24 2.45 Structure of the human smoothened 7TM receptor in complex with an antitumor agent
5BU7 2016-06-08 2.46 Crystal structure of an engineered protein that forms nanotubes with tunable diameters
4U9D 2015-01-14 2.5 Crystal Structure of the Zn-directed tetramer of the engineered cyt cb562 variant, AB3
4PXZ 2014-04-30 2.5 Crystal structure of P2Y12 receptor in complex with 2MeSADP
6IIU 2018-12-19 2.5 Crystal structure of the human thromboxane A2 receptor bound to ramatroban
5K2B 2016-09-21 2.5 2.5 angstrom A2a adenosine receptor structure with MR phasing using XFEL data
5YC8 2018-11-21 2.5 Crystal structure of rationally thermostabilized M2 muscarinic acetylcholine receptor bound with NMS (Hg-derivative)
5K2A 2016-09-21 2.5 2.5 angstrom A2a adenosine receptor structure with sulfur SAD phasing using XFEL data
3M4B 2011-01-12 2.5 A Zn-mediated tetrahedral protein lattice cage
6AQF 2018-01-10 2.51 Crystal structure of A2AAR-BRIL in complex with the antagonist ZM241385 produced from Pichia pastoris
5OLH 2018-01-17 2.6 Structure of the A2A-StaR2-bRIL562-Vipadenant complex at 2.6A obtained from in meso soaking experiments.
4QIN 2014-07-23 2.6 Structure of the human smoothened receptor in complex with SAG1.5
5ZK3 2018-11-21 2.6 Crystal structure of rationally thermostabilized M2 muscarinic acetylcholine receptor bound with QNB
3M15 2011-01-12 2.6 A Zn-mediated asymmetric trimer of a cytochrome cb562 variant (D74A-RIDC1)
4QIM 2014-07-23 2.61 Structure of the human smoothened receptor in complex with ANTA XV
4NTJ 2014-03-26 2.62 Structure of the human P2Y12 receptor in complex with an antithrombotic drug
5XZI 2017-12-20 2.65 Crystal structure of the Zn-directed tetramer of the engineered cyt cb562 variant, AB5
4IAR 2013-03-13 2.7 Crystal structure of the chimeric protein of 5-HT1B-BRIL in complex with ergotamine (PSI Community Target)
4IB4 2013-03-13 2.7 Crystal structure of the chimeric protein of 5-HT2B-BRIL in complex with ergotamine
4RWD 2015-01-14 2.7 XFEL structure of the human delta opioid receptor in complex with a bifunctional peptide
1LM3 2002-05-15 2.7 A Multi-generation Analysis of Cytochrome b562 Redox Variants: Evolutionary Strategies for Modulating Redox Potential Revealed Using a Library Approach
6G7O 2019-01-02 2.7 Crystal structure of human alkaline ceramidase 3 (ACER3) at 2.7 Angstrom resolution
6BQH 2018-02-14 2.7 Crystal structure of 5-HT2C in complex with ritanserin
6DYB 2019-04-24 2.75 Metal-free structure of the engineered cyt cb562 variant, CH3
3U8P 2012-08-29 2.75 Cytochrome b562 integral fusion with EGFP
5UNF 2017-04-05 2.8 XFEL structure of human angiotensin II type 2 receptor (Monoclinic form) in complex with compound 1 (N-benzyl-N-(2-ethyl-4-oxo-3-{[2'-(2H-tetrazol-5-yl)[1,1'-biphenyl]-4-yl])
5N2R 2017-07-26 2.8 Crystal structure of stabilized A2A adenosine receptor A2AR-StaR2-bRIL in complex with PSB36 at 2.8A resolution
4U9E 2015-01-21 2.8 Crystal structure of the Zn-directed tetramer of the engineered cyt cb562 variant, A104/57G AB3
6ME6 2019-04-24 2.8 XFEL crystal structure of human melatonin receptor MT2 in complex with 2-phenylmelatonin
3NMK 2010-11-24 2.8 Crystal structure of a zinc mediated dimer for the phenanthroline-modified cytochrome cb562 variant, MBP-Phen2
5JTB 2017-05-31 2.8 Crystal structure of the chimeric protein of A2aAR-BRIL with bound iodide ions
4NC3 2013-12-18 2.8 Crystal structure of the 5-HT2B receptor solved using serial femtosecond crystallography in lipidic cubic phase.
4OR2 2014-03-19 2.8 Human class C G protein-coupled metabotropic glutamate receptor 1 in complex with a negative allosteric modulator
5NDD 2017-05-03 2.8 Crystal structure of a thermostabilised human protease-activated receptor-2 (PAR2) in complex with AZ8838 at 2.8 angstrom resolution
4IAQ 2013-03-13 2.8 Crystal structure of the chimeric protein of 5-HT1B-BRIL in complex with dihydroergotamine (PSI Community Target)
6DHZ 2018-07-25 2.8 Crystallographic octamer of a metal-free RIDC1 variant bearing two disulfide bonded cysteines
4ZUD 2015-10-07 2.8 Crystal Structure of Human Angiotensin Receptor in Complex with Inverse Agonist Olmesartan at 2.8A resolution.
5UNG 2017-04-05 2.8 XFEL structure of human angiotensin II type 2 receptor (Orthorhombic form) in complex with compound 1 (N-benzyl-N-(2-ethyl-4-oxo-3-{[2'-(2H-tetrazol-5-yl)[1,1'-biphenyl]-4-yl] methyl}-3,4-dihydroquinazolin-6-yl)thiophene-2-carboxamide)
6C1Q 2018-05-30 2.9 Crystal structure of human C5a receptor in complex with an orthosteric antagonist PMX53 and an allosteric antagonist NDT9513727
6DO1 2019-01-30 2.9 Structure of nanobody-stabilized angiotensin II type 1 receptor bound to S1I8
2QLA 2007-11-06 2.9 Crystal Structure of a 16-Helix Bundle Architecture Produced by the Zinc-Mediated Self Assembly of Four Cytochrome cb562 Molecules
4Z36 2015-06-03 2.9 Crystal Structure of Human Lysophosphatidic Acid Receptor 1 in complex with ONO-3080573
6A94 2019-02-13 2.9 Crystal structure of 5-HT2AR in complex with zotepine
5TVN 2017-02-01 2.9 Crystal structure of the LSD-bound 5-HT2B receptor
6AK3 2018-12-05 2.9 Crystal structure of the human prostaglandin E receptor EP3 bound to prostaglandin E2
5UNH 2017-04-05 2.9 Synchrotron structure of human angiotensin II type 2 receptor in complex with compound 2 (N-[(furan-2-yl)methyl]-N-(4-oxo-2-propyl-3-{[2'-(2H-tetrazol-5-yl)[1,1'- biphenyl]-4-yl]methyl}-3,4-dihydroquinazolin-6-yl)benzamide)
4Z35 2015-06-03 2.9 Crystal Structure of Human Lysophosphatidic Acid Receptor 1 in complex with ONO-9910539
4YAY 2015-04-22 2.9 XFEL structure of human Angiotensin Receptor
6DRY 2018-08-29 2.92 Structural Determinants of Activation and Biased Agonism at the 5-HT2B Receptor
5ZKB 2018-11-21 2.95 Crystal structure of rationally thermostabilized M2 muscarinic acetylcholine receptor bound with AF-DX 384
5DHG 2015-10-21 3.0 The crystal structure of nociceptin/orphanin FQ peptide receptor (NOP) in complex with C-35 (PSI Community Target)
5TUD 2017-07-26 3.0 Structural Insights into the Extracellular Recognition of the Human Serotonin 2B Receptor by an Antibody
6A93 2019-02-13 3.0 Crystal structure of 5-HT2AR in complex with risperidone
5DHH 2015-10-21 3.0 The crystal structure of nociceptin/orphanin FQ peptide receptor (NOP) in complex with SB-612111 (PSI Community Target)
4Z34 2015-06-03 3.0 Crystal Structure of Human Lysophosphatidic Acid Receptor 1 in complex with ONO9780307
6IIV 2018-12-19 3.0 Crystal structure of the human thromboxane A2 receptor bound to daltroban
6BQG 2018-02-14 3.0 Crystal structure of 5-HT2C in complex with ergotamine
3FOP 2009-07-14 3.0 A Triangular Cytochrome b562 Superstructure Mediated by Ni Coordination - Hexagonal Form
5ZK8 2018-11-21 3.0 Crystal structure of M2 muscarinic acetylcholine receptor bound with NMS
4EA3 2012-04-25 3.01 Structure of the N/OFQ Opioid Receptor in Complex with a Peptide Mimetic
6M97 2019-04-03 3.03 Crystal structure of the high-affinity copper transporter Ctr1
6ME8 2019-04-24 3.1 XFEL crystal structure of human melatonin receptor MT2 (N86D) in complex with 2-phenylmelatonin
4PY0 2014-04-30 3.1 Crystal structure of P2Y12 receptor in complex with 2MeSATP
3NMJ 2010-11-24 3.1 Crystal structure of a nickel mediated dimer for the phenanthroline-modified cytochrome cb562 variant, MBP-Phen2
6B73 2018-01-17 3.1 Crystal Structure of a nanobody-stabilized active state of the kappa-opioid receptor
5OLO 2018-01-17 3.1 Structure of the A2A-StaR2-bRIL562-Tozadenant complex at 3.1A obtained from in meso soaking experiments.
6DRZ 2018-08-29 3.1 Structural Determinants of Activation and Biased Agonism at the 5-HT2B Receptor
6DRX 2018-08-29 3.1 Structural Determinants of Activation and Biased Agonism at the 5-HT2B Receptor
6DS0 2018-08-29 3.19 Structural Determinants of Activation and Biased Agonism at the 5-HT2B Receptor
5XJM 2018-07-11 3.2 Complex structure of angiotensin II type 2 receptor with Fab
6ME7 2019-04-24 3.2 XFEL crystal structure of human melatonin receptor MT2 (H208A) in complex with 2-phenylmelatonin
5L7D 2016-07-20 3.2 Structure of human Smoothened in complex with cholesterol
5UVI 2017-05-24 3.2 Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation
4O9R 2014-03-05 3.2 Human Smoothened Receptor structure in complex with cyclopamine
5UEN 2017-03-01 3.2 Crystal structure of the human adenosine A1 receptor A1AR-bRIL in complex with the covalent antagonist DU172 at 3.2A resolution
6M98 2019-04-03 3.21 Crystal structure of the high-affinity copper transporter Ctr1 in complex with Cu(I)
4RWA 2015-01-14 3.28 Synchrotron structure of the human delta opioid receptor in complex with a bifunctional peptide (PSI community target)
6ME9 2019-04-24 3.3 XFEL crystal structure of human melatonin receptor MT2 in complex with ramelteon
4L6R 2013-07-24 3.3 Structure of the class B human glucagon G protein coupled receptor
5N2S 2017-07-26 3.3 Crystal structure of stabilized A1 receptor in complex with PSB36 at 3.3A resolution
5L7I 2016-07-20 3.3 Structure of human Smoothened in complex with Vismodegib
6CC4 2018-06-27 3.5 Structure of MurJ from Escherichia coli
5UIG 2017-02-08 3.5 Crystal structure of adenosine A2A receptor bound to a novel triazole-carboximidamide antagonist
5NDZ 2017-05-03 3.6 Crystal structure of a thermostabilised human protease-activated receptor-2 (PAR2) in complex with AZ3451 at 3.6 angstrom resolution
6D32 2018-05-23 3.75 Crystal structure of Xenopus Smoothened in complex with cyclopamine
6D35 2018-05-23 3.9 Crystal structure of Xenopus Smoothened in complex with cholesterol
5NJ6 2017-05-03 4.0 Crystal structure of a thermostabilised human protease-activated receptor-2 (PAR2) in ternary complex with Fab3949 and AZ7188 at 4.0 angstrom resolution
6MH8 2019-04-24 4.2 High-viscosity injector-based Pink Beam Serial Crystallography of Micro-crystals at a Synchrotron Radiation Source
6CMO 2018-06-20 4.5 Rhodopsin-Gi complex

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
97.7 Soluble cytochrome b562 Q8CVG7 C562_ECOL6
97.7 Soluble cytochrome b562 P0ABE8 C562_ECO57
100.0 Soluble cytochrome b562 P0ABE7 C562_ECOLX