Myoglobins from three small placental mammals and one small marsupial were isolated from cardiac or skeletal muscle. The conformational free energies of these four myoglobins were estimated from guanidinium chloride unfolding data at pH 8 and 25 degrees. The myoglobins from rat and rabbit are more stable than that of the most stable myoglobin previously studied, that of the sperm whale. In addition, these two myoglobins unfold with greater cooperativity than previously characterized myoglobins. The data obtained herein demonstrate unequivocally for the first time that the stability of homeotherm myoglobins correlates with neither the size of the organism nor its metabolic rate. Study holds ProTherm entries: 10112, 10113, 10114, 10115 Extra Details: denaturation; guanidinium chloride; mammals;,myoglobin
ID: qf2FpHPv
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:39 p.m.
Version: 1
Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
---|
Structure ID | Release Date | Resolution | Structure Title |
---|---|---|---|
3RGK | 2011-04-08T00:00:00+0000 | 1.65 | Crystal Structure of Human Myoglobin Mutant K45R |
5YCJ | 2017-09-07T00:00:00+0000 | 1.58 | Ancestral myoglobin aMbWb' of Basilosaurus relative (polyphyly) imidazole-ligand |
5YCI | 2017-09-07T00:00:00+0000 | 1.97 | Ancestral myoglobin aMbWb' of Basilosaurus relative (polyphyly) |
1MNH | 1995-01-11T00:00:00+0000 | 2.3 | INTERACTIONS AMONG RESIDUES CD3, E7, E10 AND E11 IN MYOGLOBINS: ATTEMPTS TO SIMULATE THE O2 AND CO BINDING PROPERTIES OF APLYSIA MYOGLOBIN |
1MNJ | 1995-01-11T00:00:00+0000 | 2.2 | INTERACTIONS AMONG RESIDUES CD3, E7, E10 AND E11 IN MYOGLOBINS: ATTEMPTS TO SIMULATE THE O2 AND CO BINDING PROPERTIES OF APLYSIA MYOGLOBIN |
1MNI | 1995-01-11T00:00:00+0000 | 2.07 | ALTERATION OF AXIAL COORDINATION BY PROTEIN ENGINEERING IN MYOGLOBIN. BIS-IMIDAZOLE LIGATION IN THE HIS64-->VAL(SLASH)VAL68-->HIS DOUBLE MUTANT |
1PMB | 1989-11-27T00:00:00+0000 | 2.5 | THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT |
1YCA | 1993-08-10T00:00:00+0000 | 2.9 | DISTAL POCKET POLARITY IN LIGAND BINDING TO MYOGLOBIN: DEOXY AND CARBONMONOXY FORMS OF A THREONINE68 (E11) MUTANT INVESTIGATED BY X-RAY CRYSTALLOGRAPHY AND INFRARED SPECTROSCOPY |
1M6M | 1998-08-13T00:00:00+0000 | 1.8 | V68N MET MYOGLOBIN |
1MWD | 1998-08-11T00:00:00+0000 | 1.8 | WILD TYPE DEOXY MYOGLOBIN |
Percent Identity | Matching Chains | Protein | Accession | Entry Name |
---|---|---|---|---|
92.9 | Myoglobin | P02189 | MYG_PIG | |
90.3 | Myoglobin | P02150 | MYG_MACFA | |
90.9 | Myoglobin | P20856 | MYG_CTEGU | |
90.3 | Myoglobin | P02144 | MYG_HUMAN | |
90.9 | Myoglobin | P68085 | MYG_SEMEN | |
90.9 | Myoglobin | P68084 | MYG_PAPAN | |
90.9 | Myoglobin | P68086 | MYG_ERYPA | |
92.2 | Myoglobin | P02163 | MYG_ROUAE | |
100.0 | Myoglobin | P02193 | MYG_DIDVI | |
90.3 | Myoglobin | P02167 | MYG_NYCCO | |
93.5 | Myoglobin | P02171 | MYG_OCHPR | |
100.0 | Myoglobin | P04248 | MYG_SPAEH | |
90.3 | Myoglobin | P02145 | MYG_PANTR | |
90.9 | Myoglobin | P02166 | MYG_PERPO | |
94.2 | Myoglobin | P11343 | MYG_LUTLU | |
90.3 | Myoglobin | P02181 | MYG_INIGE | |
92.9 | Myoglobin | P02165 | MYG_TUPGL | |
100.0 | Myoglobin | P02170 | MYG_RABIT | |
90.3 | Myoglobin | C0HKB7 | MYG_SCIVU | |
90.3 | Myoglobin | P02157 | MYG_MELME | |
90.3 | Myoglobin | P02156 | MYG_ERIEU | |
90.3 | Myoglobin | P02164 | MYG_ORYAF | |
91.6 | Myoglobin | P04250 | MYG_LAGMA | |
91.6 | Myoglobin | C0HJQ9 | MYG_HYSCR | |
92.2 | Myoglobin | Q6PL31 | MYG_OCHCU | |
92.9 | Myoglobin | P04249 | MYG_PROGU |