Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves.


Abstract

Despite the central role that antibodies play in the adaptive immune system and in biotechnology, much remains unknown about the quantitative relationship between an antibody's amino acid sequence and its antigen binding affinity. Here we describe a new experimental approach, called Tite-Seq, that is capable of measuring binding titration curves and corresponding affinities for thousands of variant antibodies in parallel. The measurement of titration curves eliminates the confounding effects of antibody expression and stability that arise in standard deep mutational scanning assays. We demonstrate Tite-Seq on the CDR1H and CDR3H regions of a well-studied scFv antibody. Our data shed light on the structural basis for antigen binding affinity and suggests a role for secondary CDR loops in establishing antibody stability. Tite-Seq fills a large gap in the ability to measure critical aspects of the adaptive immune system, and can be readily used for studying sequence-affinity landscapes in other protein systems.

Submission Details

ID: pfN5Rjzn

Submitter: Paul Chang

Submission Date: Nov. 1, 2018, 5:25 p.m.

Version: 3

Publication Details
Adams RM;Mora T;Walczak AM;Kinney JB,Elife (2016) Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves. PMID:28035901
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1MH5 2003-09-23 2.1 The Structure Of The Complex Of The Fab Fragment Of The Esterolytic Antibody MS6-164 and A Transition-State Analog
1YEH 1997-12-03 2.55 STRUCTURE OF IGG2A FAB FRAGMENT
1GPO 1998-04-01 1.95 CRYSTAL STRUCTURE OF THE RATIONALLY DESIGNED ANTIBODY M41 AS A FAB FRAGMENT
1YEE 1997-10-15 2.2 STRUCTURE OF A CATALYTIC ANTIBODY, IGG2A FAB FRAGMENT (D2.5)
1YEG 1997-12-03 2.0 STRUCTURE OF IGG2A FAB FRAGMENT (D2.3) COMPLEXED WITH REACTION PRODUCT
1YEF 1997-12-03 2.0 STRUCTURE OF IGG2A FAB FRAGMENT (D2.3) COMPLEXED WITH SUBSTRATE ANALOGUE
1YEC 1997-10-15 1.9 STRUCTURE OF A CATALYTIC ANTIBODY IGG2A FAB FRAGMENT (D2.3)
1R3L 2003-11-25 2.41 potassium channel KcsA-Fab complex in Cs+
1PG7 2003-08-26 2.5 Murine 6A6 Fab in complex with humanized anti-Tissue Factor D3H44 Fab
4QNP 2015-02-11 2.8 Crystal structure of the 2009 pandemic H1N1 influenza virus neuraminidase with a neutralizing antibody
1FPT 1995-03-31 3.0 THREE-DIMENSIONAL STRUCTURE OF THE COMPLEX BETWEEN THE FAB FRAGMENT OF AN NEUTRALIZING ANTIBODY FOR TYPE 1 POLIOVIRUS AND ITS VIRAL EPITOPE
1R3J 2003-11-25 1.9 potassium channel KcsA-Fab complex in high concentration of Tl+
1MLC 1995-06-03 2.5 MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME COMPLEXED WITH LYSOZYME
1IAI 1996-03-08 2.9 IDIOTYPE-ANTI-IDIOTYPE FAB COMPLEX
1CFV 1997-10-15 2.1 MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI
1XGU 2005-09-06 2.1 Structure for antibody HyHEL-63 Y33F mutant complexed with hen egg lysozyme
1YEK 1999-01-13 2.1 CATALYTIC ANTIBODY D2.3 COMPLEX
1KEN 2002-04-24 3.5 INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH AN ANTIBODY THAT PREVENTS THE HEMAGGLUTININ LOW PH FUSOGENIC TRANSITION
15C8 1999-03-23 2.5 CATALYTIC ANTIBODY 5C8, FREE FAB
1E4W 2001-07-12 1.95 crossreactive binding of a circularized peptide to an anti-TGFalpha antibody Fab-fragment
2H8P 2006-09-12 2.25 Structure of a K channel with an amide to ester substitution in the selectivity filter
1N5Y 2003-01-28 3.1 HIV-1 Reverse Transcriptase Crosslinked to Post-Translocation AZTMP-Terminated DNA (Complex P)
1E4X 2001-07-12 1.9 crossreactive binding of a circularized peptide to an anti-TGFalpha antibody Fab-fragment
1UB5 2004-04-20 2.0 Crystal structure of Antibody 19G2 with hapten at 100K
1LO0 2002-06-26 2.0 Catalytic Retro-Diels-Alderase Transition State Analogue Complex
1A0Q 1999-03-02 2.3 29G11 COMPLEXED WITH PHENYL [1-(1-N-SUCCINYLAMINO)PENTYL] PHOSPHONATE
1KCR 2002-05-11 2.9 CRYSTAL STRUCTURE OF ANTIBODY PC283 IN COMPLEX WITH PS1 PEPTIDE
1OTT 2003-04-15 3.0 Structure of the Escherichia coli ClC Chloride channel E148A mutant and Fab Complex
1OTU 2003-04-15 3.3 Structure of the Escherichia coli ClC Chloride channel E148Q mutant and Fab Complex
2Q76 2008-03-18 2.0 Mouse anti-hen egg white lysozyme antibody F10.6.6 Fab fragment
1EHL 2001-02-21 2.4 64M-2 ANTIBODY FAB COMPLEXED WITH D(5HT)(6-4)T
1OTS 2003-04-15 2.51 Structure of the Escherichia coli ClC Chloride channel and Fab Complex
1DLF 1999-07-26 1.45 HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 5.25
2AJS 2006-02-14 1.7 Crystal structure of cocaine catalytic antibody 7A1 Fab' in complex with heptaethylene glycol
2CK0 2003-08-05 2.2 ANTI-ANTI-IDIOTYPIC ANTIBODY AGAINST HUMAN ANGIOTENSIN II, COMPLEX WITH A SYNTHETIC CYCLIC PEPTIDE
35C8 1999-03-23 2.0 CATALYTIC ANTIBODY 5C8, FAB-INHIBITOR COMPLEX
2DLF 1999-12-17 1.55 HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 6.75
1GGC 1993-10-31 2.8 MAJOR ANTIGEN-INDUCED DOMAIN REARRANGEMENTS IN AN ANTIBODY
1FAI 1992-10-15 2.7 THREE-DIMENSIONAL STRUCTURE OF TWO CRYSTAL FORMS OF FAB R19.9, FROM A MONOCLONAL ANTI-ARSONATE ANTIBODY
1GGI 1993-10-31 2.8 CRYSTAL STRUCTURE OF AN HIV-1 NEUTRALIZING ANTIBODY 50.1 IN COMPLEX WITH ITS V3 LOOP PEPTIDE ANTIGEN
1EGJ 2001-02-15 2.8 DOMAIN 4 OF THE BETA COMMON CHAIN IN COMPLEX WITH AN ANTIBODY
25C8 1999-03-23 2.0 CATALYTIC ANTIBODY 5C8, FAB-HAPTEN COMPLEX
1FNS 2000-10-18 2.0 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR (VWF) A1 DOMAIN I546V MUTANT IN COMPLEX WITH THE FUNCTION BLOCKING FAB NMC4
1IGY 1998-04-15 3.2 STRUCTURE OF IMMUNOGLOBULIN
1RUA 2004-03-02 1.75 Crystal structure (B) of u.v.-irradiated cationic cyclization antibody 4C6 fab at pH 4.6 with a data set collected at SSRL beamline 11-1.
1RUL 2004-03-02 1.88 Crystal Structure (D) of u.v.-irradiated cationic cyclization antibody 4C6 Fab at pH 5.6 with a data set collected at SSRL beamline 11-1.
1IGT 1997-07-07 2.8 STRUCTURE OF IMMUNOGLOBULIN
1BFV 1997-12-03 2.1 MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI
1RUR 2004-03-02 1.5 Crystal Structure (I) of native Diels-Alder antibody 13G5 Fab at pH 8.0 with a data set collected at SSRL beamline 9-1.
1RUP 2004-03-02 1.4 Crystal structure (G) of native cationic cyclization antibody 4C6 fab at pH 8.5 with a data set collected at APS beamline 19-ID
1RUQ 2004-03-02 1.86 Crystal Structure (H) of u.v.-irradiated Diels-Alder antibody 13G5 Fab at pH 8.0 with a data set collected in house.
4F37 2013-05-15 2.57 Structure of the tethered N-terminus of Alzheimer's disease A peptide
4KK5 2013-08-21 3.17 Structure of the CLC-ec1 deltaNC construct in 20mM fluoride and 20mM bromide
1KN4 2002-03-13 1.9 CATALYTIC ANTIBODY D2.3 COMPLEX
1KN2 2002-03-13 1.9 CATALYTIC ANTIBODY D2.3 COMPLEX
1DQQ 2000-01-19 1.8 CRYSTAL STRUCTURE OF ANTI-LYSOZYME ANTIBODY HYHEL-63
1R3I 2003-11-25 2.4 potassium channel KcsA-Fab complex in Rb+
1DQJ 2000-01-19 2.0 CRYSTAL STRUCTURE OF THE ANTI-LYSOZYME ANTIBODY HYHEL-63 COMPLEXED WITH HEN EGG WHITE LYSOZYME
5VAA 2017-06-07 1.55 Crystal structure of mouse IgG2a Fc T370K mutant
1DQM 2000-01-19 2.1 CRYSTAL STRUCTURE OF ANTI-LYSOZYME ANTIBODY
1Q9O 2004-01-27 1.79 S45-18 Fab Unliganded
1WZ1 2006-01-31 1.85 Crystal structure of the Fv fragment complexed with dansyl-lysine
1R3K 2003-11-25 2.8 potassium channel KcsA-Fab complex in low concentration of Tl+
2F19 1992-10-15 2.8 THREE-DIMENSIONAL STRUCTURE OF TWO CRYSTAL FORMS OF FAB R19.9, FROM A MONOCLONAL ANTI-ARSONATE ANTIBODY
3OZ9 2010-10-06 1.6 Crystal Structure of anti-gp41 Fab NC-1
3FO9 2009-12-22 1.9 Crystal structure of aldolase antibody 33F12 Fab' in complex with hapten 1,3-diketone
1GGB 1993-10-31 2.8 MAJOR ANTIGEN-INDUCED DOMAIN REARRANGEMENTS IN AN ANTIBODY
1KNO 1996-01-29 3.2 CRYSTAL STRUCTURE OF THE COMPLEX OF A CATALYTIC ANTIBODY FAB WITH A TRANSITION STATE ANALOG: STRUCTURAL SIMILARITIES IN ESTERASE-LIKE ABZYMES
1MAK 1994-01-31 SOLUTION STRUCTURE OF AN ISOLATED ANTIBODY VL DOMAIN
1MAJ 1994-01-31 SOLUTION STRUCTURE OF AN ISOLATED ANTIBODY VL DOMAIN
1MF2 1997-12-31 2.6 ANTI HIV1 PROTEASE FAB COMPLEX
4FAB 1990-07-15 2.7 THREE-DIMENSIONAL STRUCTURE OF A FLUORESCEIN-FAB COMPLEX CRYSTALLIZED IN 2-METHYL-2,4-PENTANEDIOL
1CIC 1999-04-19 2.5 IDIOTOPE-ANTI-IDIOTOPE FAB-FAB COMPLEX; D1.3-E225
1MNU 1999-05-06 2.5 UNLIGANDED BACTERICIDAL ANTIBODY AGAINST NEISSERIA MENINGITIDIS
1N4X 2003-06-10 1.7 Structure of scFv 1696 at acidic pH
1P2C 2004-02-17 2.0 crystal structure analysis of an anti-lysozyme antibody
1BOG 1999-03-23 2.6 ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH AN EPITOPE-HOMOLOGOUS PEPTIDE
1RU9 2004-03-02 2.5 Crystal Structure (A) of u.v.-irradiated cationic cyclization antibody 4C6 Fab at pH 4.6 with a data set collected in-house.
1PSK 1997-09-04 2.8 THE CRYSTAL STRUCTURE OF AN FAB FRAGMENT THAT BINDS TO THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE
1MLB 1995-06-03 2.1 MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME
1CK0 2003-08-05 2.5 ANTI-ANTI-IDIOTYPIC ANTIBODY AGAINST HUMAN ANGIOTENSIN II, UNLIGANDED FORM
3J3P 2013-07-03 9.1 Conformational Shift of a Major Poliovirus Antigen Confirmed by Immuno-Cryogenic Electron Microscopy: 135S Poliovirus and C3-Fab Complex
1ND0 2003-05-13 2.45 CATIONIC CYCLIZATION ANTIBODY 4C6 COMPLEX WITH TRANSITION STATE ANALOG
1FLR 1995-09-15 1.85 4-4-20 FAB FRAGMENT
1CT8 1999-11-10 2.2 CATALYTIC ANTIBODY 7C8 COMPLEX
1FE8 2001-04-04 2.03 CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING
1AXT 1998-10-28 2.15 IMMUNE VERSUS NATURAL SELECTION: ANTIBODY ALDOLASES WITH THE RATES OF NATURAL ENZYMES
2AK1 2006-02-14 1.85 Crystal Structure of Cocaine catalytic Antibody 7A1 Fab' in Complex with benzoic acid
3F7Y 2010-05-19 3.4 KcsA Potassium channel in the partially open state with 17 A opening at T112
1AHW 1998-02-25 3.0 A COMPLEX OF EXTRACELLULAR DOMAIN OF TISSUE FACTOR WITH AN INHIBITORY FAB (5G9)
1JRH 1998-03-25 2.8 COMPLEX (ANTIBODY/ANTIGEN)
3F7V 2010-05-19 3.2 KcsA Potassium channel in the open-inactivated state with 23 A opening at T112
1NCW 2003-05-13 1.3 Cationic Cyclization Antibody 4C6 in Complex with Benzoic Acid
2AJY 2006-02-14 2.1 Crystal Structure of Cocaine catalytic Antibody 7A1 Fab' in Complex with ecgonine methyl ester and benzoic acid
2AJX 2006-02-14 1.85 Crystal Structure of Cocaine catalytic Antibody 7A1 Fab' in Complex with Transition State Analog
2AJZ 2006-02-14 2.3 Crystal Structure of Cocaine catalytic Antibody 7A1 Fab' in Complex with ecgonine methyl ester
1K4D 2001-11-14 2.3 Potassium Channel KcsA-Fab complex in low concentration of K+
1NCA 1994-01-31 2.5 REFINED CRYSTAL STRUCTURE OF THE INFLUENZA VIRUS N9 NEURAMINIDASE-NC41 FAB COMPLEX
1NCB 1994-01-31 2.5 CRYSTAL STRUCTURES OF TWO MUTANT NEURAMINIDASE-ANTIBODY COMPLEXES WITH AMINO ACID SUBSTITUTIONS IN THE INTERFACE
1NCC 1994-01-31 2.5 CRYSTAL STRUCTURES OF TWO MUTANT NEURAMINIDASE-ANTIBODY COMPLEXES WITH AMINO ACID SUBSTITUTIONS IN THE INTERFACE
1NCD 1994-01-31 2.9 REFINED CRYSTAL STRUCTURE OF THE INFLUENZA VIRUS N9 NEURAMINIDASE-NC41 FAB COMPLEX
1EMT 2000-11-01 2.25 FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY
2AJV 2006-02-14 1.5 Crystal Structure of Cocaine catalytic Antibody 7A1 Fab' in Complex with Cocaine
1RUM 2004-03-02 1.48 Crystal structure (F) of H2O2-soaked cationic cyclization antibody 4C6 fab at pH 8.5 with a data set collected at SSRL beamline 9-1.
1FGN 1998-02-25 2.5 MONOCLONAL MURINE ANTIBODY 5G9-ANTI-HUMAN TISSUE FACTOR
1RUK 2004-03-02 1.4 Crystal structure (C) of native cationic cyclization antibody 4C6 fab at pH 4.6 with a data set collected at SSRL beamline 9-1
1FDL 1991-10-15 2.5 CRYSTALLOGRAPHIC REFINEMENT OF THE THREE-DIMENSIONAL STRUCTURE OF THE FAB D1.3-LYSOZYME COMPLEX AT 2.5-ANGSTROMS RESOLUTION
1SEQ 2005-03-01 1.78 Fab MNAC13
1HI6 2001-02-08 2.55 ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH A PEPTIDE
3EJZ 2009-02-17 2.9 Structure of E203V mutant E.coli Cl-/H+ exchanger, CLC-ec1
2AJU 2006-02-14 1.5 Cyrstal structure of cocaine catalytic antibody 7A1 Fab'
1FL3 2000-11-01 2.45 CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K
3FB6 2010-05-19 3.0 KcsA Potassium channel in the partially open state with 16 A opening at T112
1NDM 2003-06-03 2.1 Crystal structure of Fab fragment of antibody HyHEL-26 complexed with lysozyme
1C12 1999-08-14 2.6 INSIGHT IN ODORANT PERCEPTION: THE CRYSTAL STRUCTURE AND BINDING CHARACTERISTICS OF ANTIBODY FRAGMENTS DIRECTED AGAINST THE MUSK ODORANT TRASEOLIDE
1HH9 2001-01-12 2.7 ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH A PEPTIDE
1HH6 2001-01-26 2.6 ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH A PEPTIDE
1NDG 2003-06-03 1.9 Crystal structure of Fab fragment of antibody HyHEL-8 complexed with its antigen lysozyme
3CK0 2003-08-05 3.0 ANTI-ANTI-IDIOTYPIC ANTIBODY AGAINST HUMAN ANGIOTENSIN II, COMPLEX WITH HUMAN ANGIOTENSIN II
1OSP 1997-04-21 1.95 CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN A OF BORRELIA BURGDORFERI COMPLEXED WITH A MURINE MONOCLONAL ANTIBODY FAB
1FRG 1994-05-31 2.8 CRYSTAL STRUCTURE, SEQUENCE, AND EPITOPE MAPPING OF A PEPTIDE COMPLEX OF AN ANTI-INFLUENZA HA PEPTIDE ANTIBODY FAB 26(SLASH)9: FINE-TUNING ANTIBODY SPECIFICITY
4KK8 2013-08-21 2.86 Structure of the E148Q mutant of CLC-ec1 deltaNC construct in 100mM fluoride
1A3L 1999-02-16 1.95 CATALYSIS OF A DISFAVORED REACTION: AN ANTIBODY EXO DIELS-ALDERASE-TSA-INHIBITOR COMPLEX AT 1.95 A RESOLUTION
1AI1 1997-05-15 2.8 HIV-1 V3 LOOP MIMIC
1PLG 1996-04-03 2.8 EVIDENCE FOR THE EXTENDED HELICAL NATURE OF POLYSACCHARIDE EPITOPES. THE 2.8 ANGSTROMS RESOLUTION STRUCTURE AND THERMODYNAMICS OF LIGAND BINDING OF AN ANTIGEN BINDING FRAGMENT SPECIFIC FOR ALPHA-(2->8)-POLYSIALIC ACID
1UB6 2004-04-20 2.12 Crystal structure of Antibody 19G2 with sera ligand
3ZO0 2013-05-22 1.99 Mouse IgG2a in complex with mouse TRIM21 PRYSPRY
1CFN 1999-03-30 2.65 ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH AN EPITOPE-RELATED PEPTIDE
1KB5 1998-04-08 2.5 MURINE T-CELL RECEPTOR VARIABLE DOMAIN/FAB COMPLEX
1KCU 2002-05-11 2.2 CRYSTAL STRUCTURE OF ANTIBODY PC287
1CFT 1999-03-31 2.8 ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH AN EPITOPE-UNRELATED D-PEPTIDE
1KCV 2002-05-11 1.8 Crystal structure of antibody pc282
1CFS 1999-03-31 2.75 ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH AN EPITOPE-UNRELATED PEPTIDE
1KCS 2002-05-11 2.5 CRYSTAL STRUCTURE OF ANTIBODY PC282 IN COMPLEX WITH PS1 PEPTIDE
1CFQ 1999-03-30 2.8 ANTI-P24 (HIV-1) FAB FRAGMENT CB41
1FBI 1995-02-27 3.0 CRYSTAL STRUCTURE OF A CROSS-REACTION COMPLEX BETWEEN FAB F9.13.7 AND GUINEA-FOWL LYSOZYME
4KVC 2013-07-24 2.31 2H2 Fab fragment of immature Dengue virus
6FAB 1993-01-15 1.9 THREE-DIMENSIONAL STRUCTURE OF MURINE ANTI-P-AZOPHENYLARSONATE FAB 36-71. 1. X-RAY CRYSTALLOGRAPHY, SITE-DIRECTED MUTAGENESIS, AND MODELING OF THE COMPLEX WITH HAPTEN
1QGC 2000-01-26 STRUCTURE OF THE COMPLEX OF A FAB FRAGMENT OF A NEUTRALIZING ANTIBODY WITH FOOT AND MOUTH DISEASE VIRUS
1ORS 2003-05-06 1.9 X-ray structure of the KvAP potassium channel voltage sensor in complex with an Fab
1ORQ 2003-05-06 3.2 X-ray structure of a voltage-dependent potassium channel in complex with an Fab
1FSK 2000-10-02 2.9 COMPLEX FORMATION BETWEEN A FAB FRAGMENT OF A MONOCLONAL IGG ANTIBODY AND THE MAJOR ALLERGEN FROM BIRCH POLLEN BET V 1
1KC5 2002-07-24 2.5 CRYSTAL STRUCTURE OF ANTIBODY PC287 IN COMPLEX WITH PS1 PEPTIDE
1RIH 2004-01-13 2.5 Crystal Structure of Fab 14F7, a unique anti-tumor antibody specific for N-glycolyl GM3
1UWX 2005-06-15 2.2 P1.2 serosubtype antigen derived from N. meningitidis PorA in complex with Fab fragment
1SVZ 2005-03-01 1.89 Crystal structure of the single-chain Fv fragment 1696 in complex with the epitope peptide corresponding to N-terminus of HIV-2 protease
1CF8 1999-07-28 2.7 Convergence of catalytic antibody and terpene cyclase mechanisms: polyene cyclization directed by carbocation-pi interactions
1NBZ 2003-04-01 1.85 Crystal Structure of HyHEL-63 complexed with HEL mutant K97A
1NBY 2003-04-01 1.8 Crystal Structure of HyHEL-63 complexed with HEL mutant K96A
2HG5 2006-09-12 2.75 Cs+ complex of a K channel with an amide to ester substitution in the selectivity filter
1FJ1 2000-10-11 2.68 LYME DISEASE ANTIGEN OSPA IN COMPLEX WITH NEUTRALIZING ANTIBODY FAB LA-2
1OB1 2003-05-01 2.9 Crystal structure of a Fab complex whith Plasmodium falciparum MSP1-19
2BFV 1997-12-03 2.5 MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI
1AIF 1997-02-01 2.9 ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB FROM MOUSE

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 H 4-4-20 (IG*G2A=KAPPA=) FAB FRAGMENT P01863 GCAA_MOUSE
94.7 L 4-4-20 (IG*G2A=KAPPA=) FAB FRAGMENT P01631 KV2A7_MOUSE
100.0 L 4-4-20 (IG*G2A=KAPPA=) FAB FRAGMENT P01837 IGKC_MOUSE
100.0 H 4-4-20 (IG*G2A=KAPPA=) FAB FRAGMENT P01865 GCAM_MOUSE
94.0 H 4-4-20 (IG*G2A=KAPPA=) FAB FRAGMENT P01864 GCAB_MOUSE