Thermally induced changes in the structure and activity of yeast hexokinase B.


Yeast hexokinase has been poorly characterized in regard with its stability. In the present study, various spectroscopic techniques were employed to investigate thermal stability of the monomeric form of yeast hexokinase B (YHB). The enzyme underwent a conformational transition with a T(m) of about 41.9 degrees C. The structural transition proved to be significantly reversible below 55 degrees C and irreversible at higher temperatures. Thermoinactivation studies revealed that enzymatic activity diminished significantly at high temperatures, with greater loss of activity observed above 55 degrees C. Release of ammonia upon deamidation of YHB obeyed a similar temperature-dependence pattern. Dynamic light scattering and size exclusion-HPLC indicated formation of stable aggregates. Taking various findings on the influence of osmolytes and chaperone-like agents on YHB thermal denaturation together, it is proposed that the purely conformational transition of YHB is reversible, and irreversibility is due to aggregation, as a major cause. Deamidation of a critical Asn or Gln residue(s) may also play an important role. Study holds ProTherm entries: 25611, 25612, 25613, 25614, 25615, 25616 Extra Details: Yeast hexokinase B; Thermoinactivation; Aggregation; Deamidation; Size exclusion-HPLC

Submission Details

ID: nniaXYRV3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:55 p.m.

Version: 1

Publication Details
Ramshini H;Rezaei-Ghaleh N;Ebrahim-Habibi A;Saboury AA;Nemat-Gorgani M,Biophys. Chem. (2008) Thermally induced changes in the structure and activity of yeast hexokinase B. PMID:18715685
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Hexokinase-2 P04807 HXKB_YEAST