[Therapeutic activity and gastric tolerance of a corticosteroid associated with a gastroprotective drug].
Abstract
Study holds ProTherm entries: 15502 Extra Details: Mutation carries two substitutions, K63R and R42E and has a ~13 degree C higher than the other wild type thermostability. protein stability; hydration; packing; amino acid substitutions; differential scanning calorimetry
Submission Details
ID: nLeU7B6C
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:46 p.m.
Version: 1
Publication Details
Saccabusi E,Minerva Gastroenterol (1975) [Therapeutic activity and gastric tolerance of a corticosteroid associated with a gastroprotective drug]. PMID:1207939Additional Information
Study Summary
| Number of data points | 2 |
| Proteins | E3 ubiquitin-protein ligase RSP5 ; Ubiquitin-binding protein CUE2 |
| Unique complexes | 1 |
| Assays/Quantities/Protocols | Experimental Assay: dHcal ; Experimental Assay: ddG |
| Libraries | Mutations for sequence A:NDDHESKLSILMDMFPAISKSKLQVHLLENNNDLDLTIGLLLKENDDKS/B:MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG |
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
|---|
Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant UniProtKB Entries
| Percent Identity | Matching Chains | Protein | Accession | Entry Name |
|---|---|---|---|---|
| 100.0 | E3 ubiquitin-protein ligase RSP5 | P39940 | RSP5_YEAST | |
| 100.0 | A | Ubiquitin-binding protein CUE2 | P36075 | CUE2_YEAST |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0CH06 | RL401_SCHPO |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0CH07 | RL402_SCHPO |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0CH08 | RL40A_YEAST |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0CH09 | RL40B_YEAST |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P40909 | RL40_CRYNJ |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0CG73 | UBI1P_CANAX |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0CG74 | UBI4P_CANAX |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0CG75 | UBI4P_KLULA |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0CG72 | UBI4P_SCHPO |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0CG63 | UBI4P_YEAST |
| 98.7 | B | Ubiquitin-binding protein CUE2 | P19848 | UBIQ_COPCO |
| 98.7 | B | Ubiquitin-binding protein CUE2 | P0C224 | RL40_NEUCR |
| 98.7 | B | Ubiquitin-binding protein CUE2 | P0CG70 | UBI4P_NEUCR |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P59271 | R27AA_ARATH |
| 97.4 | B | Ubiquitin-binding protein CUE2 | B9DHA6 | RL40A_ARATH |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0CH34 | RL40A_ORYSJ |
| 97.4 | B | Ubiquitin-binding protein CUE2 | Q42202 | RL40B_ARATH |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0CH35 | RL40B_ORYSJ |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P51423 | RL40_BRARP |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P49636 | RL40_NICSY |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P62980 | RS27A_SOLLC |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P62981 | RS27A_SOLTU |
| 97.4 | B | Ubiquitin-binding protein CUE2 | Q9SHE7 | RUB1_ARATH |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0C073 | RUB1_DESAN |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0C030 | RUB1_ORYSJ |
| 97.4 | B | Ubiquitin-binding protein CUE2 | Q8RUC6 | RUB2_ARATH |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0C031 | RUB2_ORYSJ |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0CG85 | UBI1P_NICSY |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0CH04 | UBI1P_PETCR |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0CH05 | UBI2P_PETCR |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0CG84 | UBI4P_NICSY |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P69309 | UBIQP_AVEFA |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0CG83 | UBIQP_HORVU |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P69315 | UBIQP_LINUS |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P69322 | UBIQP_PEA |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P69325 | UBIQP_SOYBN |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P69310 | UBIQ_AVESA |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P69313 | UBIQ_HELAN |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P69317 | UBIQ_LUPPO |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P69326 | UBIQ_WHEAT |
| 97.4 | B | Ubiquitin-binding protein CUE2 | Q8H159 | UBQ10_ARATH |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0CH33 | UBQ11_ARATH |
| 97.4 | B | Ubiquitin-binding protein CUE2 | Q3E7T8 | UBQ14_ARATH |
| 97.4 | B | Ubiquitin-binding protein CUE2 | Q1EC66 | UBQ3_ARATH |
| 97.4 | B | Ubiquitin-binding protein CUE2 | Q58G87 | UBQ3_ORYSJ |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P0CH32 | UBQ4_ARATH |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P69061 | RS27A_KLULA |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P05759 | RS31_YEAST |
| 97.4 | B | Ubiquitin-binding protein CUE2 | P42740 | UBIQP_AGLNE |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P42739 | UBIQP_ACEPE |
| 96.1 | B | Ubiquitin-binding protein CUE2 | Q3E7K8 | UBQ12_ARATH |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CH11 | RL401_CHLRE |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CH10 | RL403_CHLRE |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P49632 | RL40_CAEEL |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG71 | UBIQ1_CAEEL |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P59669 | UBIQP_GEOCY |
| 94.7 | B | Ubiquitin-binding protein CUE2 | Q9FHQ6 | UBQ9_ARATH |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P49633 | RL40_ACACA |
| 98.7 | B | Ubiquitin-binding protein CUE2 | P14799 | RS27A_NEUCR |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P63048 | RL40_BOVIN |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P63050 | RL40_CANLF |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P18101 | RL40_DROME |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P63052 | RL40_FELCA |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P62987 | RL40_HUMAN |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0C273 | RL40_MACFA |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P62984 | RL40_MOUSE |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P68205 | RL40_OPHHA |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P63053 | RL40_PIG |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0C275 | RL40_PONPY |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P62986 | RL40_RAT |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0C276 | RL40_SHEEP |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P15357 | RS27A_DROME |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P29504 | RS27A_MANSE |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P68202 | RS27A_PLUXY |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P68203 | RS27A_SPOFR |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG53 | UBB_BOVIN |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG54 | UBB_CAVPO |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG62 | UBB_CHICK |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG67 | UBB_GORGO |
| 96.1 | B | Ubiquitin-binding protein CUE2 | Q8MKD1 | UBB_HORSE |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG47 | UBB_HUMAN |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG49 | UBB_MOUSE |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG65 | UBB_PANTR |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG60 | UBB_PONPY |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG51 | UBB_RAT |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG55 | UBB_SHEEP |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CH28 | UBC_BOVIN |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG66 | UBC_GORGO |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG48 | UBC_HUMAN |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG50 | UBC_MOUSE |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG64 | UBC_PANTR |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG68 | UBC_PIG |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG61 | UBC_PONPY |
| 96.1 | B | Ubiquitin-binding protein CUE2 | Q63429 | UBC_RAT |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P62976 | UBIQP_CRIGR |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P0CG69 | UBIQP_DROME |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P62972 | UBIQP_XENLA |
| 96.1 | B | Ubiquitin-binding protein CUE2 | Q865C5 | UBIQ_CAMDR |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P68197 | UBIQ_CERCA |
| 96.1 | B | Ubiquitin-binding protein CUE2 | P62975 | UBIQ_RABIT |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P33190 | RL40_TETPY |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0DJ25 | RL40_TETTS |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0C032 | RUB3_ORYSJ |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P23324 | UBIQP_EUPEU |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0CG82 | UBIQP_TETPY |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0CH27 | RL402_TRYCR |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P46575 | RL40_EIMBO |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P14795 | RL40_TRYCR |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P14794 | RL40_DICDI |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0CG76 | UBIQA_DICDI |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0CG77 | UBIQD_DICDI |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0CG78 | UBIQF_DICDI |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0CG79 | UBIQG_DICDI |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0CG81 | UBIQH_DICDI |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0CG80 | UBIQI_DICDI |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P0CG88 | UBIQJ_DICDI |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P22589 | UBIQP_PHYIN |
| 94.7 | B | Ubiquitin-binding protein CUE2 | P23398 | UBIQP_STRPU |
| 94.7 | B | Ubiquitin-binding protein CUE2 | Q8SWD4 | UBIQ_ENCCU |
| 96.0 | B | Ubiquitin-binding protein CUE2 | P62992 | RS27A_BOVIN |
| 96.0 | B | Ubiquitin-binding protein CUE2 | P62978 | RS27A_CAVPO |
| 96.0 | B | Ubiquitin-binding protein CUE2 | P79781 | RS27A_CHICK |
| 96.0 | B | Ubiquitin-binding protein CUE2 | P62979 | RS27A_HUMAN |
| 96.0 | B | Ubiquitin-binding protein CUE2 | P68200 | RS27A_ICTPU |
| 96.0 | B | Ubiquitin-binding protein CUE2 | P62983 | RS27A_MOUSE |
| 96.0 | B | Ubiquitin-binding protein CUE2 | P62982 | RS27A_RAT |
| 93.4 | B | Ubiquitin-binding protein CUE2 | P69201 | RL40_LEIMA |
| 92.1 | B | Ubiquitin-binding protein CUE2 | P21899 | RL40_TRYBB |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0C016 | RS27A_SCHPO |
| 100.0 | B | Ubiquitin-binding protein CUE2 | P0C8R3 | RS27B_SCHPO |
| 97.2 | B | Ubiquitin-binding protein CUE2 | Q9ARZ9 | R27AA_ORYSJ |
| 97.2 | B | Ubiquitin-binding protein CUE2 | P59232 | R27AB_ARATH |
| 97.2 | B | Ubiquitin-binding protein CUE2 | P51431 | R27AB_ORYSJ |
| 97.2 | B | Ubiquitin-binding protein CUE2 | P59233 | R27AC_ARATH |
| 97.2 | B | Ubiquitin-binding protein CUE2 | P0CG86 | RS271_HORVU |
| 97.2 | B | Ubiquitin-binding protein CUE2 | P0CG87 | RS272_HORVU |
| 97.2 | B | Ubiquitin-binding protein CUE2 | P47905 | RS27A_LUPAL |
| 97.2 | B | Ubiquitin-binding protein CUE2 | P27923 | RS27A_MAIZE |
| 93.2 | B | Ubiquitin-binding protein CUE2 | P14797 | RS27A_DICDI |
| 90.8 | B | Ubiquitin-binding protein CUE2 | P69200 | RL40_LEITA |
| 97.1 | B | Ubiquitin-binding protein CUE2 | P59272 | RS27A_DAUCA |
| 90.8 | B | Ubiquitin-binding protein CUE2 | Q39256 | UBQ8_ARATH |
| 95.2 | B | Ubiquitin-binding protein CUE2 | P84589 | UBIQ_LUMTE |
| 97.5 | B | Ubiquitin-binding protein CUE2 | P31753 | RS27A_ASPOF |