The relationship between molten globules and transient intermediates in protein folding has been explored by equilibrium and kinetic analysis of the compact acid-denatured A-state of cytochrome c. The chloride-induced formation of the A-state is a complex reaction with structural intermediates resembling those found under native refolding conditions, including a rapidly formed compact state and a subsequent intermediate with interacting N- and C-terminal helices. Together with mutational evidence for specific helix-helix packing interactions, this shows that the A-state is a stable analogue of a late folding intermediate. The L94A mutation blocks all folding steps after the initial collapse and its equilibrium state resembles early kinetic intermediates. Study holds ProTherm entries: 7416, 7417, 7418, 7419, 7420, 7421, 7422, 7423, 14391 Extra Details:
ID: n6Y3Ln2K3
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:34 p.m.
Version: 1
Number of data points | 18 |
Proteins | Cytochrome c ; Cytochrome c |
Unique complexes | 3 |
Assays/Quantities/Protocols | Experimental Assay: ddG ; Experimental Assay: Tm ; Experimental Assay: dHvH ; Experimental Assay: Cm ; Experimental Assay: dG_H2O ; Experimental Assay: Cm ; Experimental Assay: dG_H2O ; Derived Quantity: dTm |
Libraries | Mutations for sequence GDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFTYTDANKNKGITWKEETLMEYLENPKKYIPGTKMIFAGIKKKTEREDLIAYLKKATNE |
Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
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Structure ID | Release Date | Resolution | Structure Title |
---|---|---|---|
1KTD | 2002-01-15T00:00:00+0000 | 2.4 | CRYSTAL STRUCTURE OF CLASS II MHC MOLECULE IEK BOUND TO PIGEON CYTOCHROME C PEPTIDE |
2B4Z | 2005-09-27T00:00:00+0000 | 1.5 | Crystal structure of cytochrome C from bovine heart at 1.5 A resolution. |
2YBB | 2011-03-02T00:00:00+0000 | 19.0 | Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) |
3J2T | 2012-12-23T00:00:00+0000 | 9.5 | An improved model of the human apoptosome |
5C0Z | 2015-06-12T00:00:00+0000 | 1.12 | The structure of oxidized rat cytochrome c at 1.13 angstroms resolution |
5C9M | 2015-06-28T00:00:00+0000 | 1.36 | The structure of oxidized rat cytochrome c (T28A) at 1.362 angstroms resolution. |
5DF5 | 2015-08-26T00:00:00+0000 | 1.3 | The structure of oxidized rat cytochrome c (T28E) at 1.30 angstroms resolution. |
5JUY | 2016-05-10T00:00:00+0000 | 4.1 | Active human apoptosome with procaspase-9 |
6FF5 | 2018-01-03T00:00:00+0000 | 1.74 | X-ray structure of bovine heart cytochrome c at high ionic strength |
6N1O | 2018-11-09T00:00:00+0000 | 1.55 | Oxidized rat cytochrome c mutant (S47E) |
Percent Identity | Matching Chains | Protein | Accession | Entry Name |
---|---|---|---|---|
100.0 | Cytochrome c | P00004 | CYC_HORSE | |
99.0 | Cytochrome c | P68097 | CYC_EQUAS | |
99.0 | Cytochrome c | P68096 | CYC_EQUBU | |
97.1 | Cytochrome c | P62894 | CYC_BOVIN | |
97.1 | Cytochrome c | P62895 | CYC_PIG | |
97.1 | Cytochrome c | P62896 | CYC_SHEEP | |
94.3 | Cytochrome c | P00007 | CYC_HIPAM | |
95.2 | Cytochrome c | P68099 | CYC_CAMDR | |
95.2 | Cytochrome c | P68100 | CYC_ESCRO | |
95.2 | Cytochrome c | P68098 | CYC_LAMGU | |
94.3 | Cytochrome c | P62897 | CYC_MOUSE | |
94.3 | Cytochrome c | P00008 | CYC_RABIT | |
94.3 | Cytochrome c | P62898 | CYC_RAT | |
94.3 | Cytochrome c | P00011 | CYC_CANLF | |
93.3 | Cytochrome c | P00014 | CYC_MACGI | |
93.3 | Cytochrome c | P00013 | CYC_MINSC | |
93.3 | Cytochrome c | Q52V09 | CYC_CEPBA | |
93.3 | Cytochrome c | P00012 | CYC_MIRLE | |
90.5 | Cytochrome c | Q52V10 | CYC_SAISC | |
92.3 | Cytochrome c | P81280 | CYC_ALLMI | |
92.2 | Cytochrome c | P00020 | CYC_ANAPL | |
91.3 | Cytochrome c | P00021 | CYC_COLLI | |
90.3 | Cytochrome c | B4USV4 | CYC_OTOGA |