Co-solvent effects on structure and function properties of savinase: solvent-induced thermal stabilization.


Abstract

The industrial utilization of savinase is mainly constrained by its stability limitations. In the present study, the irreversible thermoinactivation of savinase has been evaluated at 70 degrees C, and various possible mechanisms for irreversible thermoinactivation of savinase were examined. The main process seemed to be autodigestion of savinase at higher temperatures. To improve the thermal stability of the enzyme, the effect of two co-solvents (sorbitol and trehalose) on the enzyme's activity and stability was investigated. Both osmolytes prevented the autolysis of savinase at 70 degrees C without inactivating the enzyme; furthermore, the structural and kinetic stabilities of the enzyme increased in the presence of additives. Study holds ProTherm entries: 25410, 25411, 25412 Extra Details: Savinase; Thermal stability; Co-solvent; Autolysis; Aggregation

Submission Details

ID: n4EpwK5y3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:55 p.m.

Version: 1

Publication Details
Nasiripourdori A;Naderi-Manesh H;Ranjbar B;Khajeh K,Int. J. Biol. Macromol. (2009) Co-solvent effects on structure and function properties of savinase: solvent-induced thermal stabilization. PMID:18955077
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1AH2 1998-04-15 SERINE PROTEASE PB92 FROM BACILLUS ALCALOPHILUS, NMR, 18 STRUCTURES
1GCI 1998-10-21 0.78 THE 0.78 ANGSTROMS STRUCTURE OF A SERINE PROTEASE-BACILLUS LENTUS SUBTILISIN
5AQE 2015-10-07 1.1 Cooperative bio-metallic selectivity in a tailored protease enables creation of a C-C cross-coupling Heckase
5ARC 2016-09-14 1.1 Cooperative bio-metallic selectivity in a tailored protease enables creation of a C-C cross-coupling Heckase
5ARB 2016-09-14 1.15 Cooperative bio-metallic selectivity in a tailored protease enables creation of a C-C cross-coupling Heckase
4CFY 2014-04-09 1.17 SAVINASE CRYSTAL STRUCTURES FOR COMBINED SINGLE CRYSTAL DIFFRACTION AND POWDER DIFFRACTION ANALYSIS
4CG0 2014-04-09 1.36 Savinase crystal structures for combined single crystal diffraction and powder diffraction analysis
1SVN 1996-10-14 1.4 SAVINASE
1ST3 1994-01-31 1.4 THE CRYSTAL STRUCTURE OF THE BACILLUS LENTUS ALKALINE PROTEASE, SUBTILISIN BL, AT 1.4 ANGSTROMS RESOLUTION
1C9N 1999-10-06 1.5 BACILLUS LENTUS SUBSTILISIN VARIANT (SER 87) K27R/V104Y/N123S/T274A
1WSD 2004-11-16 1.5 Alkaline M-protease form I crystal strcuture
1TK2 2004-06-22 1.54 Crystal Structure of the Complex formed between Alkaline Proteinase Savinase and Gramicidin S at 1.5A Resolution
5ARD 2016-09-14 1.55 Cooperative bio-metallic selectivity in a tailored protease enables creation of a C-C cross-coupling Heckase
4CFZ 2014-04-09 1.57 SAVINASE CRYSTAL STRUCTURES FOR COMBINED SINGLE CRYSTAL DIFFRACTION AND POWDER DIFFRACTION ANALYSIS
1Q5P 2003-11-11 1.6 S156E/S166D variant of Bacillus lentus subtilisin
1NDU 2004-04-06 1.6 Bacillus lentus subtilisin variant S101G/V104N
1C9M 1999-10-06 1.67 BACILLUS LENTUS SUBTILSIN (SER 87) N76D/S103A/V104I
1IAV 2001-04-18 1.8 STRUCTURE ON NATIVE (ASN 87) SUBTILISIN FROM BACILLUS LENTUS
1NDQ 2004-04-06 1.8 Bacillus lentus subtilisin
1C9J 1999-10-06 1.8 BACILLUS LENTUS SUBTILISIN K27R/N87S/V104Y/N123S/T274A VARIANT
3BX1 2008-07-08 1.85 Complex between the Barley alpha-Amylase/Subtilisin Inhibitor and the subtilisin Savinase
1JEA 1997-11-26 2.0 ALTERED TOPOLOGY AND FLEXIBILITY IN ENGINEERED SUBTILISIN
1MPT 1994-06-22 2.4 CRYSTAL STRUCTURE OF A NEW ALKALINE SERINE PROTEASE (M-PROTEASE) FROM BACILLUS SP. KSM-K16

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
98.1 Subtilisin Savinase P29599 SUBB_BACLE
98.9 Subtilisin Savinase Q99405 PRTM_BACSK
99.6 Subtilisin Savinase P27693 ELYA_BACAO
100.0 Subtilisin Savinase P29600 SUBS_BACLE
100.0 Subtilisin Savinase P41362 ELYA_BACCS