Discovery of high affinity anti-ricin antibodies by B cell receptor sequencing and by yeast display of combinatorial VH:VL libraries from immunized animals.


Abstract

Ricin is a toxin that could potentially be used as a bioweapon. We identified anti-ricin A chain antibodies by sequencing the antibody repertoire from immunized mice and by selecting high affinity antibodies using yeast surface display. These methods led to the isolation of multiple antibodies with high (sub-nanomolar) affinity. Interestingly, the antibodies identified by the 2 independent approaches are from the same clonal lineages, indicating for the first time that yeast surface display can identify native antibodies. The new antibodies represent well-characterized reagents for biodefense diagnostics and therapeutics development.

Submission Details

ID: mpRMHKrs

Submitter: Marie Ary

Submission Date: Oct. 29, 2018, 11:12 a.m.

Version: 1

Publication Details
Wang B;Lee CH;Johnson EL;Kluwe CA;Cunningham JC;Tanno H;Crooks RM;Georgiou G;Ellington AD,MAbs (2016) Discovery of high affinity anti-ricin antibodies by B cell receptor sequencing and by yeast display of combinatorial VH:VL libraries from immunized animals. PMID:27224530
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
4HAF 2012-09-26T00:00:00+0000 2.04 Crystal structure of fc-fragment of human IgG2 antibody (primitive crystal form)
4HAG 2012-09-26T00:00:00+0000 3.4 Crystal structure of fc-fragment of human IgG2 antibody (centered crystal form)
4L4J 2013-06-07T00:00:00+0000 1.92 Crystal structure of fc-fragment of human IgG2-Sigma antibody
7LUS 2021-02-23T00:00:00+0000 2.45 IgG2 Fc Charge Pair Mutation version 1 (CPMv1)
7LUS 2021-02-23T00:00:00+0000 2.45 IgG2 Fc Charge Pair Mutation version 1 (CPMv1)
1ADQ 1997-02-18T00:00:00+0000 3.15 CRYSTAL STRUCTURE OF A HUMAN IGM RHEUMATOID FACTOR FAB IN COMPLEX WITH ITS AUTOANTIGEN IGG FC
1BBJ 1992-04-30T00:00:00+0000 3.1 CRYSTAL STRUCTURE OF A CHIMERIC FAB' FRAGMENT OF AN ANTIBODY BINDING TUMOUR CELLS
3EO1 2008-09-26T00:00:00+0000 3.1 Structure of the Fab Fragment of GC-1008 in Complex with Transforming Growth Factor-Beta 3
4B53 2012-08-02T00:00:00+0000 1.8 Crystal structure of the isolated IgG4 CH3 domain
4C54 2013-09-10T00:00:00+0000 1.9 Crystal structure of recombinant human IgG4 Fc

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Anti-ricin scAb P0DOX7 IGK_HUMAN
100.0 Anti-ricin scAb P01834 IGKC_HUMAN
94.7 Anti-ricin scAb P01602 KV105_HUMAN
100.0 H Anti-ricin chimeric Ab P01857 IGHG1_HUMAN
90.9 H Anti-ricin chimeric Ab P01859 IGHG2_HUMAN
90.9 H Anti-ricin chimeric Ab P01861 IGHG4_HUMAN