Reversible oligomerization and denaturation of the chaperonin GroES.


Abstract

The chaperonin GroEL can assist protein folding and normally acts with the co-chaperonin GroES. These Escherichia coli proteins are encoded on the same operon, with GroES positioned first. In this report, we have investigated the reversible folding of GroES. Using fluorescence anisotropy of dansyl-labeled GroES, intrinsic fluorescence, bis-ANS binding, sedimentation velocity, and limited proteolysis, we show that GroES unfolds in a single, two-state transition. Importantly, intrinsic fluorescence and sedimentation velocity analyses show that GroES is capable of refolding and reassembling from a urea denatured state. The refolded GroES is fully active as shown by its ability to assist GroEL in the refolding of rhodanese. These results indicate that chaperonins may not require other chaperonins for successful folding/assembly. We also show that GroES is capable of assisting in the refolding/reassembly of fully denatured GroEL. The reversible folding of GroES coupled with the ability of GroES to assist the refolding/reassembly of GroEL suggest that the groE operon may be organized in a manner that provides a structural role in GroES/GroEL assembly as well as a functional role. Study holds ProTherm entries: 5016 Extra Details: two-state transition; chaperonins; structural role;,functional role

Submission Details

ID: kMYDhaYA4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:28 p.m.

Version: 1

Publication Details
Seale JW;Gorovits BM;Ybarra J;Horowitz PM,Biochemistry (1996) Reversible oligomerization and denaturation of the chaperonin GroES. PMID:8672442
Additional Information

Study Summary

Number of data points 2
Proteins 60 kDa chaperonin ; 10 kDa chaperonin
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: m ; Experimental Assay: dG_H2O
Libraries Mutations for sequence A:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/B:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/C:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/D:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/E:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/F:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/G:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/H:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/I:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/J:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/K:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/L:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/M:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/N:AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM/O:MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA/P:MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA/Q:MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA/R:MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA/S:MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA/T:MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA/U:MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1EGS 1997-09-17 NMR STRUCTURE OF GROES MOBILE LOOP RESIDUES 19-27 IN THE SYNTHETIC PEPTIDE (RESIDUES 13-32) BOUND TO GROEL, 20 STRUCTURES
3VZ6 2013-11-13 1.5 Crystal Structure Analysis of the Mini-chaperonines, variant with Gly 184 replaced with Ile and Leu 185 replaced Val and Val 186 replaced with Leu.
1KID 1997-09-17 1.7 GROEL (HSP60 CLASS) FRAGMENT (APICAL DOMAIN) COMPRISING RESIDUES 191-376, MUTANT WITH ALA 262 REPLACED WITH LEU AND ILE 267 REPLACED WITH MET
3VZ7 2013-11-13 1.8 Crystal Structure Analysis of the mini-chaperonin variant with Pro 187 Gly
3VZ8 2013-11-13 1.9 Crystal Structure Analysis of the Mini-chaperonin variant with Leu 185, Val 186, Pro 187, Arg 188 and Ser 190 replaced with all Gly
1GRU 2002-01-28 12.5 SOLUTION STRUCTURE OF GROES-ADP7-GROEL-ATP7 COMPLEX BY CRYO-EM
2C7E 2006-02-16 14.9 REVISED ATOMIC STRUCTURE FITTING INTO A GROEL(D398A)-ATP7 CRYO-EM MAP (EMD 1047)
3ZQ1 2013-06-19 15.9 Visualizing GroEL-ES in the Act of Encapsulating a Non-Native Substrate Protein
1SX3 2005-03-01 2.0 GroEL14-(ATPgammaS)14
1KP8 2003-03-25 2.0 Structural Basis for GroEL-assisted Protein Folding from the Crystal Structure of (GroEL-KMgATP)14 at 2.0 A Resolution
1DK7 2000-01-05 2.02 CRYSTAL STRUCTURE OF AN ISOLATED APICAL DOMAIN OF GROEL
1LA1 2002-04-03 2.06 Gro-EL Fragment (Apical Domain) Comprising Residues 188-379
1DKD 2000-01-12 2.1 CRYSTAL STRUCTURE OF A GROEL (APICAL DOMAIN) AND A DODECAMERIC PEPTIDE COMPLEX
1FY9 2000-11-22 2.2 CRYSTAL STRUCTURE OF THE HEXA-SUBSTITUTED MUTANT OF THE MOLECULAR CHAPERONIN GROEL APICAL DOMAIN
1FYA 2000-11-22 2.2 CRYSTAL STRUCTURE OF THE HEXA-SUBSTITUTED MUTANT OF THE MOLECULAR CHAPERONIN GROEL APICAL DOMAIN
1JON 1997-03-12 2.5 GROEL (HSP60 CLASS) FRAGMENT COMPRISING RESIDUES 191-345
1SS8 2005-03-01 2.7 GroEL
1OEL 1996-04-03 2.8 CONFORMATIONAL VARIABILITY IN THE REFINED STRUCTURE OF THE CHAPERONIN GROEL AT 2.8 ANGSTROM RESOLUTION
1GRL 1995-10-15 2.8 THE CRYSTAL STRUCTURE OF THE BACTERIAL CHAPERONIN GROEL AT 2.8 ANGSTROMS
1PCQ 2003-10-14 2.81 Crystal structure of groEL-groES
1SVT 2005-03-01 2.81 Crystal structure of GroEL14-GroES7-(ADP-AlFx)7
1XCK 2005-10-25 2.92 Crystal structure of apo GroEL
1PF9 2003-11-04 2.99 GroEL-GroES-ADP
1MNF 2003-10-07 3.0 Domain motions in GroEL upon binding of an oligopeptide
1AON 1997-10-15 3.0 CRYSTAL STRUCTURE OF THE ASYMMETRIC CHAPERONIN COMPLEX GROEL/GROES/(ADP)7
1SX4 2005-03-01 3.0 GroEL-GroES-ADP7
2NWC 2007-05-22 3.02 A 3.02 angstrom crystal structure of wild-type apo GroEL in a monoclinic space group
4WSC 2015-11-11 3.04 Crystal structure of a GroELK105A mutant
4WGL 2015-09-30 3.13 Crystal structure of a GroEL D83A/R197A double mutant
5OPW 2018-01-10 3.19 Crystal structure of the GroEL mutant A109C
2EU1 2006-08-29 3.29 Crystal structure of the chaperonin GroEL-E461K
5W0S 2017-08-09 3.5 GroEL using cryoEM
4V43 2014-07-09 3.52 Structural and mechanistic basis for allostery in the bacterial chaperonin GroEL
5OPX 2018-01-10 3.64 Crystal structure of the GroEL mutant A109C in complex with GroES and ADP BeF2
3WVL 2014-09-17 3.79 Crystal structure of the football-shaped GroEL-GroES complex (GroEL: GroES2:ATP14) from Escherichia coli
3E76 2009-08-25 3.94 Crystal structure of Wild-type GroEL with bound Thallium ions
3CAU 2008-09-02 4.2 D7 symmetrized structure of unliganded GroEL at 4.2 Angstrom resolution by cryoEM
2YEY 2011-05-18 4.5 Crystal structure of the allosteric-defective chaperonin GroEL E434K mutant
3C9V 2008-09-02 4.7 C7 Symmetrized Structure of Unliganded GroEL at 4.7 Angstrom Resolution from CryoEM
2C7C 2006-01-25 7.7 FITTED COORDINATES FOR GROEL-ATP7-GROES CRYO-EM COMPLEX (EMD-1180)
1GR5 2002-01-28 7.9 Solution Structure of apo GroEL by Cryo-Electron microscopy
4AAQ 2012-12-12 8.0 ATP-triggered molecular mechanics of the chaperonin GroEL
4AAR 2012-12-12 8.0 ATP-triggered molecular mechanics of the chaperonin GroEL
2CGT 2006-03-29 8.2 GROEL-ADP-gp31 COMPLEX
2YNJ 2012-12-12 8.4 GroEL at sub-nanometer resolution by Constrained Single Particle Tomography
4AB2 2012-12-12 8.5 ATP-triggered molecular mechanics of the chaperonin GroEL
4AAS 2012-12-12 8.5 ATP-triggered molecular mechanics of the chaperonin GroEL
4AAU 2012-12-12 8.5 ATP-triggered molecular mechanics of the chaperonin GroEL
4AB3 2012-12-12 8.5 ATP-triggered molecular mechanics of the chaperonin GroEL
2C7D 2006-01-25 8.7 Fitted coordinates for GroEL-ADP7-GroES Cryo-EM complex (EMD-1181)
3ZPZ 2013-06-19 8.9 Visualizing GroEL-ES in the Act of Encapsulating a Non-Native Substrate Protein
3ZQ0 2013-06-19 9.2 Visualizing GroEL-ES in the Act of Encapsulating a Non-Native Substrate Protein

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
641.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin Q2NW95 CH10_SODGM
649.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin Q7MAZ6 CH10_PHOLL
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin A8G8S6 CH10_SERP5
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin P0A1D5 CH10_SALTY
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin P0A1D6 CH10_SALTI
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin B4TSC5 CH10_SALSV
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin B5BKF3 CH10_SALPK
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin Q5PL63 CH10_SALPA
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin B4TF79 CH10_SALHS
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin B5R990 CH10_SALG2
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin B5R004 CH10_SALEP
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin B5FRK1 CH10_SALDC
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin A9MFS0 CH10_SALAR
634.9000000000001 O,P,Q,R,S,T,U 60 kDa chaperonin B5F2K9 CH10_SALA4
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin C5BDK4 CH10_EDWI9
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin A6TH52 CH10_KLEP7
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin P48228 CH10_YEREN
649.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin Q93FU7 CH10_KLEAE
649.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin A4W5N7 CH10_ENT38
649.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin A1JIP2 CH10_YERE8
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin B1JMR2 CH10_YERPY
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin Q66FD6 CH10_YERPS
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin A4TRR1 CH10_YERPP
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin Q1CED3 CH10_YERPN
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin A9QYQ2 CH10_YERPG
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin Q8ZIY4 CH10_YERPE
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin B2K1Y3 CH10_YERPB
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin Q1C0X9 CH10_YERPA
642.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin A7FN02 CH10_YERP3
656.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin B5Y369 CH10_KLEP3
649.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin A7MMB9 CH10_CROS8
671.3 O,P,Q,R,S,T,U 60 kDa chaperonin P95801 CH10_STEMA
656.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin B4EXE3 CH10_PROMH
663.5999999999999 O,P,Q,R,S,T,U 60 kDa chaperonin Q6D9J1 CH10_PECAS
671.3 O,P,Q,R,S,T,U 60 kDa chaperonin C6DKC6 CH10_PECCP
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin Q3YUJ8 CH10_SHISS
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin P0A6G2 CH10_SHIFL
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin Q0SXD7 CH10_SHIF8
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin Q328C5 CH10_SHIDS
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin Q31T77 CH10_SHIBS
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B2TY17 CH10_SHIB3
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B7LLS4 CH10_ESCF3
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin Q1R3B7 CH10_ECOUT
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B1LQG3 CH10_ECOSM
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B6I614 CH10_ECOSE
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B7NG80 CH10_ECOLU
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin P0A6F9 CH10_ECOLI
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B1ITQ6 CH10_ECOLC
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin P0A6G0 CH10_ECOL6
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin Q0T9P9 CH10_ECOL5
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin A8A7N8 CH10_ECOHS
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B1XDP6 CH10_ECODH
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin C5A1D4 CH10_ECOBW
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B7M8Q3 CH10_ECO8A
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B7MSG3 CH10_ECO81
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B7NTK1 CH10_ECO7I
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B5Z2F1 CH10_ECO5E
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin P0A6G1 CH10_ECO57
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B7LC01 CH10_ECO55
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B7MKU7 CH10_ECO45
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin B7UPW2 CH10_ECO27
700.0 O,P,Q,R,S,T,U 60 kDa chaperonin A7ZV11 CH10_ECO24
1261.3999999999999 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q8KIX2 CH60_BUCTS
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin P0C193 CH60_SODGL
1278.1999999999996 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A1JIP3 CH60_YERE8
1285.1999999999996 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q2NW94 CH60_SODGM
1281.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin P48219 CH60_YEREN
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B1JMR1 CH60_YERPY
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q66FD5 CH60_YERPS
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A4TRR0 CH60_YERPP
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q1CED4 CH60_YERPN
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A9QYQ1 CH60_YERPG
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q8ZIY3 CH60_YERPE
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B2K1Y4 CH60_YERPB
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q1C0Y0 CH60_YERPA
1283.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A7FN01 CH60_YERP3
1292.1999999999996 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q6D9J0 CH60_PECAS
1302.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B2VL84 CH60_ERWT9
1310.3999999999999 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66204 CH60_SERFI
1318.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66206 CH60_SERMA
1313.1999999999996 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66222 CH60_ERWAP
1307.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66202 CH60_SERRU
1310.3999999999999 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin C5BDK5 CH60_EDWI9
1339.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin P95800 CH60_STEMA
1320.1999999999996 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66218 CH60_PANAN
1331.3999999999999 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A8G8S7 CH60_SERP5
1339.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66026 CH60_KLEPN
1339.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66220 CH60_PECCC
1344.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66210 CH60_KLEOX
1344.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin C6DKC7 CH60_PECCP
1344.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66200 CH60_ENTAG
1352.3999999999999 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A7MMC0 CH60_CROS8
1344.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66214 CH60_RAOOR
1346.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A6TH53 CH60_KLEP7
1346.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B5Y368 CH60_KLEP3
1349.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66190 CH60_ENTAS
1346.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66198 CH60_KLEAK
1355.1999999999996 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A4W5N8 CH60_ENT38
1363.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66194 CH60_PLUGE
1358.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66196 CH60_LELAM
1365.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66212 CH60_RAOPL
1365.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A8AMQ6 CH60_CITK8
1367.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin O66192 CH60_KLUIN
1381.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B5R991 CH60_SALG2
1381.8000000000004 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A9MFR9 CH60_SALAR
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin P0A1D3 CH60_SALTY
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin P0A1D4 CH60_SALTI
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B4TSC6 CH60_SALSV
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B5BKF4 CH60_SALPK
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin C0Q6A2 CH60_SALPC
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A9N3Z7 CH60_SALPB
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q5PL62 CH60_SALPA
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B4TF80 CH60_SALHS
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B5R005 CH60_SALEP
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B5FRK2 CH60_SALDC
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q57GP7 CH60_SALCH
1384.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B5F2L0 CH60_SALA4
1391.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B7LLS5 CH60_ESCF3
1394.3999999999999 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q0T9P8 CH60_ECOL5
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q3YUJ7 CH60_SHISS
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin P0A6F8 CH60_SHIFL
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q0SXD6 CH60_SHIF8
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q328C4 CH60_SHIDS
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q31T78 CH60_SHIBS
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B2TY18 CH60_SHIB3
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin Q1R3B6 CH60_ECOUT
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B1LQG4 CH60_ECOSM
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B6I615 CH60_ECOSE
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B7NG81 CH60_ECOLU
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin P0A6F5 CH60_ECOLI
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B1ITQ5 CH60_ECOLC
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin P0A6F6 CH60_ECOL6
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A8A7N9 CH60_ECOHS
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B1XDP7 CH60_ECODH
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin C5A1D5 CH60_ECOBW
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B7M8Q4 CH60_ECO8A
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B7MSV9 CH60_ECO81
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B7NTK2 CH60_ECO7I
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B5Z2F2 CH60_ECO5E
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin P0A6F7 CH60_ECO57
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B7LC02 CH60_ECO55
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B7MKU8 CH60_ECO45
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin B7UPW3 CH60_ECO27
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A7ZV12 CH60_ECO24
1400.0 A,B,C,D,E,F,G,H,I,J,K,L,M,N 60 kDa chaperonin A1AJ51 CH601_ECOK1