Two-stage thermal unfolding of [Cys55]-substituted Cro repressor of bacteriophage lambda.


Abstract

It has been shown by scanning calorimetry and 1H NMR spectroscopy that thermal denaturation of mutant lambda phage cro repressor in which Val55 was substituted for Cys, proceeds in 2 stages in contrast to the wild type protein. At neutral pH values, an additional cooperative transition has been observed at about 100 degrees C. Calorimetric measurements on the mutant and its tryptic fragment lead to the conclusion that the two-stage character of thermal unfolding of the mutant is due to a disruption of an additional cooperative domain in the dimer molecule which is stabilized by the S-S crosslink. Study holds ProTherm entries: 10812, 10813, 10814 Extra Details: cro repressor; mutant; thermal denaturation; S-S bond;,scanning calorimetry; 1H NMR

Submission Details

ID: kFhG5QLP4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:41 p.m.

Version: 1

Publication Details
Gitelson GI;Griko Yu V;Kurochkin AV;Rogov VV;Kutyshenko VP;Kirpichnikov MP;Privalov PL,FEBS Lett. (1991) Two-stage thermal unfolding of [Cys55]-substituted Cro repressor of bacteriophage lambda. PMID:1833238
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
2A63 2006-06-06 Solution structure of a stably monomeric mutant of lambda Cro produced by substitutions in the ball-and-socket interface
1COP 1995-10-15 THREE-DIMENSIONAL DIMER STRUCTURE OF THE LAMBDA-CRO REPRESSOR IN SOLUTION AS DETERMINED BY HETERONUCLEAR MULTIDIMENSIONAL NMR
1ZUG 1997-07-07 STRUCTURE OF PHAGE 434 CRO PROTEIN, NMR, 20 STRUCTURES
2ORC 1998-05-27 CRO REPRESSOR INSERTION MUTANT K56-[DGEVK], NMR, 32 STRUCTURES
2OVG 2008-01-08 1.35 Lambda Cro Q27P/A29S/K32Q triple mutant at 1.35 A in space group P3221
2ECS 2008-01-08 1.4 Lambda Cro mutant Q27P/A29S/K32Q at 1.4 A in space group C2
1ORC 1996-12-23 1.54 CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]
1D1M 1999-09-24 2.05 CRYSTAL STRUCTURE OF CRO K56-[DGEVK]-F58W MUTANT
1D1L 1999-10-06 2.1 CRYSTAL STRUCTURE OF CRO-F58W MUTANT
5CRO 1998-06-17 2.3 REFINED STRUCTURE OF CRO REPRESSOR PROTEIN FROM BACTERIOPHAGE LAMBDA
2CRO 1989-10-15 2.35 STRUCTURE OF PHAGE 434 CRO PROTEIN AT 2.35 ANGSTROMS RESOLUTION
3CRO 1991-10-15 2.5 THE PHAGE 434 CRO/OR1 COMPLEX AT 2.5 ANGSTROMS RESOLUTION
6CRO 1998-09-18 3.0 CRYSTAL STRUCTURE OF LAMBDA-CRO BOUND TO A CONSENSUS OPERATOR AT 3.0 ANGSTROM RESOLUTION
3ORC 1998-12-02 3.0 CRYSTAL STRUCTURE OF AN ENGINEERED CRO MONOMER BOUND NONSPECIFICALLY TO DNA
4CRO 1992-01-15 3.9 PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
93.0 Regulatory protein cro P03040 RCRO_LAMBD
100.0 Regulatory protein cro P03036 RCRO_BP434