Calorimetric studies of the energetics of protein-DNA interactions in the E. coli methionine repressor (MetJ) system.


Abstract

Calorimetric measurements of binding of a specific DNA fragment and S-adenosyl methionine (SAM) co-repressor molecules to the E. coli methionine repressor (MetJ) show significant differences in the energetics of binary and ternary protein-DNA complexes. Formation of the MetJ:SAM:DNA ternary complex is significantly more exothermic (delta H congruent to -99 kJ.mol-1) than either MetJ:DNA or MetJ:SAM binary complexes alone (delta H congruent to -10 kJ.mol-1 each). The protein is also significantly more stable to unfolding (delta Tm congruent to 5.4 degrees C) when bound to DNA. These observations suggest that binding of SAM to the protein-DNA complex leads to a significant reduction in dynamic flexibility of the ternary complex, with considerable entropy-enthalpy compensation, not necessarily involving any overall conformational change. Study holds ProTherm entries: 10393 Extra Details: protein-DNA interaction; calorimetry; energetics; dynamics; methionine repressor

Submission Details

ID: kES6wamV3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:40 p.m.

Version: 1

Publication Details
Cooper A;McAlpine A;Stockley PG,FEBS Lett. (1994) Calorimetric studies of the energetics of protein-DNA interactions in the E. coli methionine repressor (MetJ) system. PMID:8026581
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1CMC 1993-10-31 1.8 THREE DIMENSIONAL CRYSTAL STRUCTURES OF E. COLI MET REPRESSOR WITH AND WITHOUT COREPRESSOR
1CMB 1993-10-31 1.8 THREE DIMENSIONAL CRYSTAL STRUCTURES OF ESCHERICHIA COLI MET REPRESSOR WITH AND WITHOUT COREPRESSOR
1MJL 1998-04-29 2.1 METHIONINE REPRESSOR MUTANT (Q44K) COMPLEX WITH THE COREPRESSOR SAM (S-ADENOSYL METHIONINE) FROM ESCHERICHIA COLI
1MJO 1999-08-02 2.1 METHIONINE HOLOREPRESSOR MUTANT (Q44K) PLUS COREPRESSOR (S-ADENOSYL METHIONINE) COMPLEXED TO THE MINIMAL MET CONSENSUS OPERATOR WITH THE CENTRAL TA STEP MUTATED TO AT
1MJK 1998-05-27 2.15 METHIONINE REPRESSOR MUTANT APOREPRESSOR (Q44K) FROM ESCHERICHIA COLI
1MJM 1999-08-02 2.2 METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO HALF OF THE CONSENSUS OPERATOR SEQUENCE
1MJQ 1999-08-02 2.4 METHIONINE REPRESSOR MUTANT (Q44K) PLUS COREPRESSOR (S-ADENOSYL METHIONINE) COMPLEXED TO AN ALTERED MET CONSENSUS OPERATOR SEQUENCE
1MJ2 1999-08-02 2.4 METHIONINE REPRESSOR MUTANT (Q44K) PLUS COREPRESSOR (S-ADENOSYL METHIONINE) COMPLEXED TO A CONSENSUS OPERATOR SEQUENCE
1CMA 1993-10-31 2.8 MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE
1MJP 1999-08-02 3.4 METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO THE MINIMAL MET CONSENSUS OPERATOR

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
90.5 Met repressor B4F178 METJ_PROMH
90.5 Met repressor B1JQ68 METJ_YERPY
90.5 Met repressor Q66G78 METJ_YERPS
90.5 Met repressor B2JZD3 METJ_YERPB
90.5 Met repressor A7FCZ2 METJ_YERP3
90.5 Met repressor A1JI14 METJ_YERE8
90.5 Met repressor A8GL90 METJ_SERP5
93.3 Met repressor A8AKY5 METJ_CITK8
92.4 Met repressor B2VI92 METJ_ERWT9
91.4 Met repressor Q2NQY6 METJ_SODGM
91.4 Met repressor Q7MYC7 METJ_PHOLL
93.3 Met repressor C6DHN7 METJ_PECCP
93.3 Met repressor Q6CZA0 METJ_PECAS
94.3 Met repressor A7ML79 METJ_CROS8
94.3 Met repressor A4WG61 METJ_ENT38
94.3 Met repressor A6TGC7 METJ_KLEP7
95.2 Met repressor B5XZ31 METJ_KLEP3
98.1 Met repressor P06203 METJ_SALTY
99.0 Met repressor Q8Z2Z4 METJ_SALTI
99.0 Met repressor B4TPW1 METJ_SALSV
99.0 Met repressor B5BJL6 METJ_SALPK
99.0 Met repressor C0Q452 METJ_SALPC
99.0 Met repressor A9MZJ3 METJ_SALPB
99.0 Met repressor Q5PK48 METJ_SALPA
99.0 Met repressor B4T0U7 METJ_SALNS
99.0 Met repressor B4TCN8 METJ_SALHS
99.0 Met repressor B5RF72 METJ_SALG2
99.0 Met repressor B5QXM8 METJ_SALEP
99.0 Met repressor B5FPU9 METJ_SALDC
99.0 Met repressor Q57HB6 METJ_SALCH
99.0 Met repressor B5F0T1 METJ_SALA4
100.0 Met repressor Q3YV35 METJ_SHISS
100.0 Met repressor P0A8U9 METJ_SHIFL
100.0 Met repressor Q32AD6 METJ_SHIDS
100.0 Met repressor Q31U49 METJ_SHIBS
100.0 Met repressor B2TWD5 METJ_SHIB3
100.0 Met repressor B7LUR5 METJ_ESCF3
100.0 Met repressor B1LNP3 METJ_ECOSM
100.0 Met repressor B6I4T6 METJ_ECOSE
100.0 Met repressor P0A8U6 METJ_ECOLI
100.0 Met repressor B1IVD9 METJ_ECOLC
100.0 Met repressor P0A8U7 METJ_ECOL6
100.0 Met repressor Q0TAC6 METJ_ECOL5
100.0 Met repressor A8A746 METJ_ECOHS
100.0 Met repressor B1XBA4 METJ_ECODH
100.0 Met repressor C5A0A5 METJ_ECOBW
100.0 Met repressor B7M6Z3 METJ_ECO8A
100.0 Met repressor B5Z040 METJ_ECO5E
100.0 Met repressor P0A8U8 METJ_ECO57
100.0 Met repressor B7LA39 METJ_ECO55
100.0 Met repressor B7UNQ8 METJ_ECO27
100.0 Met repressor A7ZUF7 METJ_ECO24