Effects of nucleotide binding on thermal transitions and domain structure of myosin subfragment 1.


Abstract

The thermal unfolding and domain structure of myosin subfragment 1 (S1) from rabbit skeletal muscles and their changes induced by nucleotide binding were studied by differential scanning calorimetry. The binding of ADP to S1 practically does not influence the position of the thermal transition (maximum at 47.2 degrees C), while the binding of the non-hydrolysable analogue of ATP, adenosine 5'-[beta, gamma-imido]triphosphate (AdoPP[NH]P) to S1, or trapping of ADP in S1 by orthovanadate (Vi), shift the maximum of the heat adsorption curve for S1 up to 53.2 and 56.1 degrees C, respectively. Such an increase of S1 thermostability in the complexes S1-AdoPP[NH]P and S1-ADP-Vi is confirmed by results of turbidity and tryptophan fluorescence measurements. The total heat adsorption curves for S1 and its complexes with nucleotides were decomposed into elementary peaks corresponding to the melting of structural domains in the S1 molecule. Quantitative analysis of the data shows that the domain structure of S1 in the complexes S1-AdoPP[NH]P and S1-ADP-Vi is similar and differs radically from that of nucleotide-free S1 and S1 in the S1-ADP complex. These data are the first direct evidence that the S1 molecule can be in two main conformations which may correspond to different states during the ATP hydrolysis: one of them corresponds to nucleotide-free S1 and to the complex S1-ADP, and the other corresponds to the intermediate complexes S1-ATP and S1-ADP-Pi. Surprisingly it turned out that the domain structure of S1 with ADP trapped by p-phenylene-N, N'-dimaleimide (pPDM) thiol cross-linking almost does not differ from that of the nucleotide-free S1. This means that pPDM-cross-linked S1 in contrast to S1-AdoPP[NH]P and S1-ADP-Vi can not be considered a structural analogue of the intermediate complexes S1-ATP and S1-ADP-Pi. Study holds ProTherm entries: 7607, 7608, 7609, 7610, 7611 Extra Details: S1: myosin subfragment 1

Submission Details

ID: jxT7aMc24

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:34 p.m.

Version: 1

Publication Details
Levitsky DI;Shnyrov VL;Khvorov NV;Bukatina AE;Vedenkina NS;Permyakov EA;Nikolaeva OP;Poglazov BF,Eur. J. Biochem. (1992) Effects of nucleotide binding on thermal transitions and domain structure of myosin subfragment 1. PMID:1425691
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


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4E50 2013-03-20 2.7 Calmodulin and Ng peptide complex
6K4K 2019-07-24 2.71 Crystal structure of SidJ-CaM binary complex at 2.71 A
1A29 1998-09-16 2.74 CALMODULIN COMPLEXED WITH TRIFLUOPERAZINE (1:2 COMPLEX)
4BYF 2014-03-26 2.74 Crystal structure of human Myosin 1c in complex with calmodulin in the pre-power stroke state
1K90 2002-01-23 2.75 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin and 3' deoxy-ATP
3DVJ 2008-11-04 2.8 Crystal Structure of Ca2+/CaM-CaV2.2 IQ domain (without cloning artifact, HM to TV) complex
3EKJ 2008-12-16 2.8 Calcium-free GCaMP2 (calcium binding deficient mutant)
4OVN 2014-12-03 2.8 Voltage-gated Sodium Channel 1.5 (Nav1.5) C-terminal domain in complex with Calmodulin poised for activation
3EK8 2008-12-16 2.8 Calcium-saturated GCaMP2 T116V/G87R mutant monomer
5HIT 2016-09-14 2.85 Crystal Structure Analysis of Ca2+-calmodulin and a C-terminal EAG1 channel fragment
4V0C 2014-11-05 2.86 Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin
1DEG 1994-05-31 2.9 THE LINKER OF DES-GLU84 CALMODULIN IS BENT AS SEEN IN THE CRYSTAL STRUCTURE
2BKI 2005-06-07 2.9 Myosin VI nucleotide-free (MDinsert2-IQ) crystal structure
1K93 2002-01-23 2.95 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin
6K4L 2019-07-24 2.95 Crystal structure of Se-labelled SidJ complex with CaM at 2.95 A
3J41 2013-07-31 25.0 Pseudo-atomic model of the Aquaporin-0/Calmodulin complex derived from electron microscopy
4IL1 2013-10-02 3.0 Crystal Structure of the Rat Calcineurin
1S26 2004-04-13 3.0 Structure of Anthrax Edema Factor-Calmodulin-alpha,beta-methyleneadenosine 5'-triphosphate Complex Reveals an Alternative Mode of ATP Binding to the Catalytic Site
4UMO 2014-11-05 3.0 Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin
5WSU 2017-06-07 3.0 Crystal structure of Myosin VIIa IQ5-SAH in complex with apo-CaM
4JPZ 2014-04-16 3.02 Voltage-gated sodium channel 1.2 C-terminal domain in complex with FGF13U and Ca2+/calmodulin
1QX7 2004-08-31 3.09 Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel
6K4R 2019-07-24 3.11 Crystal structure of SidJ-CaM-AMP ternary complex at 3.11 A
5V7X 2018-02-28 3.14 Crystal Structure of Myosin 1b residues 1-728 with bound sulfate and Calmodulin
5I0I 2016-09-07 3.15 Crystal structure of myosin X motor domain with 2IQ motifs in pre-powerstroke state
6C1D 2018-01-31 3.2 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
1XFX 2005-05-03 3.2 Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 10 millimolar exogenously added calcium chloride
1SK6 2004-06-08 3.2 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin, 3',5' cyclic AMP (cAMP), and pyrophosphate
1XFZ 2005-05-03 3.25 Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 1 millimolar exogenously added calcium chloride
1XFY 2005-05-03 3.3 Crystal structure of anthrax edema factor (EF) in complex with calmodulin
6MC9 2019-04-10 3.3 Crystal Structure of Human Nav1.4 C-Terminal (1599-1754) domain in complex with calcium-bound calmodulin
6O20 2019-04-24 3.3 Cryo-EM structure of TRPV5 with calmodulin bound
1PK0 2004-02-10 3.3 Crystal Structure of the EF3-CaM complexed with PMEApp
1XFV 2005-05-03 3.35 Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3' deoxy-ATP
1XFU 2005-05-03 3.35 Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex with calmodulin
1XFW 2005-05-03 3.4 Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3'5' cyclic AMP (cAMP)
6CNM 2018-05-02 3.4 Cryo-EM structure of the human SK4/calmodulin channel complex
6CNN 2018-05-02 3.5 Cryo-EM structure of the human SK4/calmodulin channel complex in the Ca2+ bound state I
4R8G 2014-12-03 3.5 Crystal Structure of Myosin-1c tail in complex with Calmodulin
6E2G 2018-08-22 3.6 Cryo-EM structure of rat TRPV6 in complex with Calmodulin
1Y0V 2005-05-03 3.6 Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate
1LVC 2002-12-04 3.6 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin and 2' deoxy, 3' anthraniloyl ATP
5VMS 2017-06-07 3.7 CryoEM structure of Xenopus KCNQ1 channel
5K7L 2016-08-17 3.78 Single particle cryo-EM structure of the voltage-gated K+ channel Eag1 bound to the channel inhibitor calmodulin
6C1G 2018-01-31 3.8 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
4JQ0 2014-05-21 3.84 Voltage-gated sodium channel 1.5 C-terminal domain in complex with FGF12B and Ca2+/calmodulin
6C1H 2018-01-31 3.9 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
6E2F 2018-08-22 3.9 Cryo-EM structure of human TRPV6 in complex with Calmodulin
6S5T 2019-07-24 4.15 Legionella pneumophila SidJ-Human calmodulin complex
6DMW 2018-10-24 4.4 Calmodulin-bound full-length rbTRPV5
6BNV 2018-01-10 4.6 CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine
6CNO 2018-05-02 4.7 Cryo-EM structure of the human SK4/calmodulin channel complex in the Ca2+ bound state II

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 B Calmodulin P20689 MYLK2_RAT
100.0 B Calmodulin P07313 MYLK2_RABIT
100.0 B Calmodulin Q8VCR8 MYLK2_MOUSE
100.0 B Calmodulin Q9H1R3 MYLK2_HUMAN
100.0 B Calmodulin A4IFM7 MYLK2_BOVIN
93.5 A Calmodulin P18061 CALM_TRYCR
93.5 A Calmodulin P69098 CALM_TRYBG
93.5 A Calmodulin P69097 CALM_TRYBB
100.0 A Calmodulin P62184 CALM_RENRE
100.0 A Calmodulin P11121 CALM_PYUSP
100.0 A Calmodulin P02595 CALM_PATSP
92.3 A Calmodulin P53440 CALMF_NAEGR
94.2 A Calmodulin P11118 CALM_EUGGR
100.0 A Calmodulin P02594 CALM_ELEEL
91.4 A Calmodulin P05932 CALMB_ARBPU
96.5 A Calmodulin Q9HFY6 CALM_BLAEM
100.0 A Calmodulin Q40302 CALM_MACPY
91.9 A Calmodulin P62150 CALM_ORYLA
95.7 A Calmodulin O02367 CALM_CIOIN
91.2 A Calmodulin P27166 CALM_STYLE
91.2 A Calmodulin O97341 CALM_SUBDO
91.2 A Calmodulin A8I1Q0 CALM_HETTR
92.6 A Calmodulin P02598 CALM_TETPY
92.6 A Calmodulin A3E4D8 CALM_PROMN
92.6 A Calmodulin A3E3H0 CALM_PFIPI
92.6 A Calmodulin A3E4F9 CALM_KARVE
92.6 A Calmodulin A4UHC0 CALM_ALEFU
92.6 A Calmodulin O96081 CALMB_HALRO
92.6 A Calmodulin P62146 CALMA_ARBPU
98.3 A Calmodulin Q95NR9 CALM_METSE
97.4 A Calmodulin Q7T3T2 CALM_EPIAK
98.3 A Calmodulin P0DP35 CAM2B_XENLA
98.3 A Calmodulin P0DP34 CAM2A_XENLA
98.3 A Calmodulin P62151 CALM_TETCF
98.3 A Calmodulin P21251 CALM_STIJA
98.3 A Calmodulin Q6YNX6 CALM_SHEEP
98.3 A Calmodulin P62160 CALM_RABIT
98.3 A Calmodulin Q5RAD2 CALM_PONAB
98.3 A Calmodulin Q71UH6 CALM_PERFV
98.3 A Calmodulin P62156 CALM_ONCSP
98.3 A Calmodulin Q6PI52 CALM_DANRE
98.3 A Calmodulin Q6IT78 CALM_CTEID
98.3 A Calmodulin P62149 CALM_CHICK
98.3 A Calmodulin P62157 CALM_BOVIN
98.3 A Calmodulin P62144 CALM_ANAPL
98.3 A Calmodulin P0DP31 CALM3_RAT
98.3 A Calmodulin P0DP28 CALM3_MOUSE
98.3 A Calmodulin P0DP25 CALM3_HUMAN
98.3 A Calmodulin P0DP30 CALM2_RAT
98.3 A Calmodulin P0DP27 CALM2_MOUSE
98.3 A Calmodulin P0DP24 CALM2_HUMAN
99.1 A Calmodulin Q9UB37 CALM2_BRALA
98.3 A Calmodulin P0DP33 CALM1_XENLA
98.3 A Calmodulin P0DP29 CALM1_RAT
98.3 A Calmodulin P0DP26 CALM1_MOUSE
98.3 A Calmodulin P0DP23 CALM1_HUMAN
98.0 A Calmodulin Q8STF0 CALM_STRIE
99.3 A Calmodulin P62154 CALM_LOCMI
99.3 A Calmodulin P62152 CALM_DROME
99.3 A Calmodulin P62145 CALM_APLCA
99.3 A Calmodulin P62153 CALMA_HALRO
99.3 A Calmodulin P62148 CALM1_BRALA
99.3 A Calmodulin P62147 CALM1_BRAFL
98.6 A Calmodulin O16305 CALM_CAEEL