Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity.


Abstract

The Gibbs free energy difference between native and unfolded states ("stability") is one of the fundamental characteristics of a protein. By exploiting the thermodynamic linkage between ligand binding and stability, interactions of a protein with small molecules, nucleic acids, or other proteins can be detected and quantified. Determination of protein stability can therefore provide a universal monitor of biochemical function. Yet, the use of stability measurements as a functional probe is underutilized, because such experiments traditionally require large amounts of protein and special instrumentation. Here we present the quantitative cysteine reactivity (QCR) technique to determine protein stabilities rapidly and accurately using only picomole quantities of material and readily accessible laboratory equipment. We demonstrate that QCR-derived stabilities can be used to measure ligand binding over a wide range of ligand concentrations and affinities. We anticipate that this technique will have broad applications in high-throughput protein engineering experiments and functional genomics. Study holds ProTherm entries: 25489, 25490, 25491, 25492, 25493, 25494, 25495, 25496, 25497, 25498 Extra Details: conformational stability; thermal stability; ligand-binding affinity; linkage analysis; thiol protection

Submission Details

ID: joyYVDep3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:55 p.m.

Version: 1

Publication Details
Isom DG;Vardy E;Oas TG;Hellinga HW,Proc. Natl. Acad. Sci. U.S.A. (2010) Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity. PMID:20194783
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1JOQ 2001-08-22 Ensemble structures for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate
1JOK 2001-08-22 Averaged structure for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate
2PQE 2007-06-19 Solution structure of proline-free mutant of staphylococcal nuclease
2M00 2013-03-06 Solution structure of staphylococcal nuclease E43S mutant in the presence of ssDNA and Cd2+
2LKV 2012-09-12 Staphylococcal Nuclease PHS variant
2KHS 2009-10-20 Solution structure of SNase121:SNase(111-143) complex
1JOR 2001-08-22 Ensemble structures for unligated Staphylococcal nuclease-H124L
2FXZ 2006-12-19 Solution structure of 97-109 segment of staphylococcal nuclease
2SOB 1995-12-07 SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES
1JOO 2001-08-22 Averaged structure for unligated Staphylococcal nuclease-H124L
4HMJ 2012-10-31 1.35 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D at cryogenic temperature
4EQP 2012-05-02 1.35 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D at cryogenic temperature
4N9P 2013-12-04 1.4 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L25A/V99T at cryogenic temperature
4KY5 2013-06-12 1.4 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D at pH 7 and cryogenic temperature
3TME 2011-09-14 1.4 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E at cryogenic temperature
3SXH 2011-07-27 1.4 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92AL103A at cryogenic temperature
4DFA 2012-02-01 1.4 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L36A at cryogenic temperature
5JOB 2016-05-11 1.45 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92A at cryogenic temperature
3SR1 2011-07-27 1.45 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature
4DGZ 2012-02-08 1.47 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L125A at cryogenic temperature
3SHL 2011-06-29 1.48 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74KL25A at cryogenic temperature
3LX0 2011-02-16 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N at cryogenic temperature
3MEH 2011-03-16 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A at cryogenic temperature
5KEE 2016-06-22 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92F at cryogenic temperature
5HUR 2016-02-10 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25T/I92K at cryogenic temperature
2SNS 1982-07-29 1.5 STAPHYLOCOCCAL NUCLEASE. PROPOSED MECHANISM OF ACTION BASED ON STRUCTURE OF ENZYME-THYMIDINE 3(PRIME),5(PRIME)-BIPHOSPHATE-CALCIUM ION COMPLEX AT 1.5-ANGSTROMS RESOLUTION
4DF7 2012-02-01 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23L/V99I at cryogenic temperature
4NDX 2013-11-13 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N at cryogenic temperature
4NP5 2013-12-04 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92N at cryogenic temperature
5DAU 2015-09-02 1.5 Crystal structure of Staphylococcal nuclease variant Delta+PHS V39E/V104E at cryogenic temperature
4KY7 2013-06-12 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74D at cryogenic temperature
4K2L 2013-04-24 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92Q at cryogenic temperature
4KV6 2013-06-12 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS R126Q at cryogenic temperature
4KJN 2013-05-15 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66A/V99T at cryogenic temperature
4KD4 2013-05-08 1.55 Crystal structure of Staphylococcal nuclease variant V23L/L25V/V66I/I72V at cryogenic temperature
3MHB 2011-03-23 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS L38A at cryogenic temperature
3VA5 2012-01-25 1.55 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V23A at cryogenic temperature
4LAA 2013-08-21 1.58 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36H at cryogenic temperature
3MZ5 2011-03-23 1.58 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103A at cryogenic temperature
5CV8 2015-08-12 1.58 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/T62H at cryogenic temperature
3RUZ 2011-06-15 1.58 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74K at cryogenic temperature
3QOJ 2011-03-16 1.6 Cryogenic structure of Staphylococcal nuclease variant D+PHS/V23K
5KRU 2016-07-27 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66K at cryogenic temperature
4QB4 2014-06-11 1.6 Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L
3TP8 2011-09-21 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E at cryogenic temperature
3OSO 2011-09-21 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A at cryogenic temperature
1EY0 2000-10-18 1.6 STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.6 A RESOLUTION
2DRI 1995-01-26 1.6 PROBING PROTEIN-PROTEIN INTERACTIONS: THE RIBOSE BINDING PROTEIN IN BACTERIAL TRANSPORT AND CHEMOTAXIS
3PMF 2011-11-16 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23A at cryogenic temperature
4H7B 2012-10-17 1.6 Crystal Structure of Staphylococcal nuclease mutant I72V/V99L
4IAL 2013-01-09 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS H121E at cryogenic temperature
4N9T 2013-10-30 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92S at cryogenic temperature
4KHV 2013-05-15 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S at cryogenic temperature
4NKL 2013-11-20 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92Q at cryogenic temperature
4IUN 2013-02-06 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS R126E at cryogenic temperature
4TRD 2014-07-16 1.6 Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K/V74K at pH 9.0 at cryogenic temperature
4J6H 2013-03-06 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS H121Q at cryogenic temperature
5IOD 2016-03-23 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99E at cryogenic temperature
4K14 2013-04-17 1.6 Crystal Structure of Staphylococcal nuclease mutant V66I/V99L
1EY4 2000-10-18 1.6 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S59A
4F8M 2012-06-13 1.6 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23I/I92V at cryogenic temperature
4WOR 2014-10-29 1.6 Staphylococcal nuclease in complex with Ca2+ and thymidine-3'-5'-diphosphate (pdTp) at room temperature
4I65 2013-03-20 1.61 Crystal Structure of Staphylococcal nuclease mutant V23I/L25V/I72V/I92V
3MXP 2011-03-23 1.61 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature
5C4Z 2015-07-15 1.62 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25H at cryogenic temperature
5CV9 2015-08-12 1.62 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/V66H at cryogenic temperature
3QB3 2012-01-18 1.63 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92KL25A at cryogenic temperature
4DU9 2012-03-07 1.63 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V74A at cryogenic temperature
4S3S 2015-07-01 1.64 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K/V23A at cryogenic temperature
4YIJ 2015-03-18 1.64 Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature
4K5X 2013-04-24 1.65 Crystal structure of Staphylococcal nuclease variant V23M/L36F at cryogenic temperature
4PMC 2014-06-11 1.65 Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K V74K at cryogenic temperature
2EY6 2006-10-24 1.65 Crystal structure of Staphylococcal nuclease mutant T41V
4K6D 2013-04-24 1.65 Crystal structure of Staphylococcal nuclease variant V23M/T62F at cryogenic temperature
1IHZ 2003-06-17 1.65 Structure of S. nuclease mutant quintuple mutant V23L/V66L/I72L/I92L/V99L
3NXW 2011-07-20 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS L125A at cryogenic temperature
1SNC 1990-10-15 1.65 THE CRYSTAL STRUCTURE OF THE TERNARY COMPLEX OF STAPHYLOCOCCAL NUCLEASE, CA2+, AND THE INHIBITOR PD*TP, REFINED AT 1.65 ANGSTROMS
4K2K 2013-04-24 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/L36A/I92A at cryogenic temperature
5CV7 2015-08-12 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92D at cryogenic temperature
6B8R 2017-10-18 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36Q at cryogenic temperature
4J1M 2013-02-20 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS R105E at cryogenic temperature
4R8N 2014-09-17 1.65 Crystal structure of Staphylococcal nuclease variant V23I/V66I/I72V/I92V at cryogenic temperature
4WRD 2015-07-08 1.65 Crystal structure of Staphylcoccal nulease variant Delta+PHS V66E L125E at cryogenic temperature
4K5W 2013-04-24 1.65 Crystal structure of Staphylococcal nuclease variant V23M/L25F/T62F at cryogenic temperature
3NK9 2011-06-29 1.65 Crystal structure of Staphylococcal nuclease variant Delta+PHS V74A at cryogenic temperature
4RKL 2014-11-26 1.66 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66T at cryogenic temperature
4OL7 2015-01-28 1.67 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E A109E at cryogenic temperature
4MIU 2013-10-02 1.67 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V99T at cryogenic temperature
1STY 1993-04-15 1.67 THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE
4PNY 2014-03-12 1.68 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72H at cryogenic temperature
2F0T 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant V66L/V99I
2EYL 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant T82S
3NP8 2011-06-29 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A at cryogenic temperature
5KYI 2016-08-03 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 9 at cryogenic temperature
5F2D 2015-12-16 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N/V99T at cryogenic temperature
4IZ8 2013-02-06 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS H8E at cryogenic temperature
4F7X 2012-06-13 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L25A at cryogenic temperature
1STN 1994-07-31 1.7 THE CRYSTAL STRUCTURE OF STAPHYLOCOCCAL NUCLEASE REFINED AT 1.7 ANGSTROMS RESOLUTION
5C5M 2015-07-08 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D / V104E at cryogenic temperature
4KY6 2013-06-12 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25D at pH 6 and cryogenic temperature
5IGF 2016-03-09 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V66D at cryogenic temperature
3H6M 2010-03-09 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E at cryogenic temperature
1EYD 2000-10-18 1.7 STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.7 A RESOLUTION
1EY5 2000-10-18 1.7 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V
1STA 1994-06-22 1.7 ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE
5DEH 2015-09-02 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS N100D at cryogenic temperature
1SNQ 1997-02-12 1.7 PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE
1SYD 1994-07-31 1.7 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
5IGG 2016-03-09 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I72E at cryogenic temperature
4NMZ 2013-12-11 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23Q/V66A at cryogenic temperature
2EY2 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant T41C
2F0N 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant L25I/I72L
4EQO 2012-05-02 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V99D at cryogenic temperature
1STG 1995-01-26 1.7 TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES
3SK5 2011-07-06 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D at cryogenic temperature
1SNO 1997-02-12 1.7 PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE
4ZUI 2015-05-27 1.7 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23H at cryogenic temperature
2EYO 2006-10-24 1.7 Crystal structure of Staphylococcal nuclease mutant T120S
4ZUJ 2015-05-27 1.72 Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91H at cryogenic temperature
1EY9 2000-10-18 1.72 STRUCTURE OF S. NUCLEASE STABILIZING QUADRUPLE MUTANT T41I/P117G/H124L/S128A
1II3 2003-06-17 1.72 Structure of S. nuclease quintuple mutant V23I/V66L/I72L/I92L/V99L
5C3W 2015-07-15 1.72 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H at cryogenic temperature
3MVV 2011-03-16 1.72 Crystal structure of Staphylococcal nuclease variant Delta+PHS F34A at cryogenic temperature
4ODG 2014-01-29 1.73 Crystal structure of Staphylococcal nuclease variant V23I/V66I/V74I/V99I at cryogenic temperature
5J1Z 2016-04-06 1.73 Crystal structure of Staphylococcal nuclease variant Delta+PHS A90D at cryogenic temperature
1SNM 1991-07-15 1.74 ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES
4HTH 2012-11-28 1.75 Crystal structure of Staphylococcal nuclease variant Delta+VIAGLA at cryogenic temperature
4K5V 2013-04-24 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L36A/V99T at cryogenic temperature
4KD3 2013-05-08 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/V66A/I92A at cryogenic temperature
5C5L 2015-07-08 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E / L125E at cryogenic temperature
1EY8 2000-10-18 1.75 STRUCTURE OF S. NUCLEASE STABILIZING TRIPLE MUTANT P117G/H124L/S128A
2F0S 2006-10-24 1.75 Crystal structure of Staphylococcal nuclease mutant V66L/I92V
3ITP 2010-07-21 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS F34K at cryogenic temperature
5E3F 2015-10-14 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K at cryogenic temperature
4HMI 2012-10-31 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS V99K at cryogenic temperature
5HGT 2016-01-20 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92T at cryogenic temperature
1EY6 2000-10-18 1.75 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T41I
5KIX 2016-06-29 1.75 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92E at cryogenic temperature
3P1H 2011-10-05 1.79 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/I92A at cryogenic temperature
5IGC 2016-03-09 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/V66H at cryogenic temperature
1SYB 1994-07-31 1.8 TRANSFER OF A BETA-TURN STRUCTURE TO A NEW PROTEIN CONTEXT
5IGD 2016-03-09 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperature
5C4H 2015-07-15 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92H at cryogenic temperature
6OK8 2019-05-08 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS K127L at cryogenic temperature
2EYF 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant T44V
3T16 2011-08-03 1.8 Crystal structure of Staphylococcal nuclease variant NVIAGA/M98G at cryogenic temperature
1SYC 1994-07-31 1.8 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
3DMU 2008-11-04 1.8 Crystal structure of Staphylococcal nuclease variant PHS T62K at cryogenic temperature
5CR3 2015-08-05 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E/L125E at cryogenic temperature
5CV5 2015-08-12 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS K64G/V66K/E67G at cryogenic temperature
2F0I 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant I72L
1KAB 1994-01-31 1.8 STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE
3T13 2011-08-03 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G at cryogenic temperature
5CV4 2015-08-12 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/V66H at cryogenic temperature
4PMB 2014-06-11 1.8 Crystal structure of Staphylcoccal nuclease variant Delta+PHS I92S at cryogenic temperature
1F2Z 2003-09-16 1.8 SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
3TP6 2011-09-21 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/L103K at cryogenic temperature
3BDC 2008-11-04 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS at cryogenic temperature
2EYP 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant T120V
5IGB 2016-03-09 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D/V66H at cryogenic temperature
5IGE 2016-03-09 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V99D at cryogenic temperature
3V2T 2011-12-28 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V66A at cryogenic temperature
3HEJ 2010-05-19 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature
1SYE 1994-07-31 1.8 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
4QF4 2014-06-04 1.8 Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature
4EQN 2012-05-02 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/I72K at cryogenic temperature
2F0J 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant I72V
1SYF 1994-07-31 1.8 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
3SK6 2011-07-06 1.8 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23K/L36E at cryogenic temperature
2EYM 2006-10-24 1.8 Crystal structure of Staphylococcal nuclease mutant T120C
3TP5 2011-09-21 1.8 Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E/L36E at cryogenic temperature
4ME5 2013-10-23 1.8 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S/V66A at cryogenic temperature
5K5P 2016-06-01 1.83 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K L103K at cryogenic temperature
2F0U 2006-10-24 1.83 Crystal structure of Staphylococcal nuclease mutant V23I/L25I/I72V
1SND 1997-04-21 1.84 STAPHYLOCOCCAL NUCLEASE DIMER CONTAINING A DELETION OF RESIDUES 114-119 COMPLEXED WITH CALCIUM CHLORIDE AND THE COMPETITIVE INHIBITOR DEOXYTHYMIDINE-3',5'-DIPHOSPHATE
1TT2 2004-07-06 1.85 Cryogenic crystal structure of Staphylococcal nuclease variant truncated Delta+PHS I92K
6AMF 2017-09-06 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36E at cryogenic temperature
5CGK 2015-07-22 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66D/N100E at cryogenic temperature
5I9P 2016-03-09 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H at cryogenic temperature
5KYL 2016-08-03 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 7 at cryogenic temperature
3OWF 2010-10-20 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66R at cryogenic temperature
5EGT 2015-11-11 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E at cryogenic temperature
1STH 1995-02-27 1.85 TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES
3SK4 2011-07-06 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N/V23E at cryogenic temperature
3ERO 2008-11-04 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72E at cryogenic temperature
5IIF 2016-03-09 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D at cryogenic temperature
2EY1 2006-10-24 1.85 Crystal structure of Staphylococcal nuclease mutant T22V
5C6A 2015-07-08 1.85 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E / N100E at cryogenic temperature
1EYC 2000-10-18 1.85 STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T41I/S59A/P117G/H124L/S128A
2EYJ 2006-10-24 1.85 Crystal structure of Staphylococcal nuclease mutant T62V
1EZ8 2000-10-18 1.85 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V
6OKA 2019-05-08 1.86 Crystal structure of Staphylococcal nuclease variant Delta+PHS Q123D at cryogenic temperature
1EY7 2000-10-18 1.88 STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S128A
4RKB 2014-11-26 1.88 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T/V99T at cryogenic temperature
5JAV 2016-04-27 1.89 Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91D at cryogenic temperature
3P75 2010-11-17 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104D at cryogenic temperature
4ID6 2013-03-06 1.9 Crystal Structure of Staphylococcal nuclease mutant V23I/I72L
5IOC 2016-03-23 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99D at cryogenic temperature
5J22 2016-04-06 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103D at cryogenic temperature
3S9W 2011-06-22 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature
1SYG 1994-07-31 1.9 ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE
5ISR 2016-03-23 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L38E at cryogenic temperature
1KDA 1995-02-27 1.9 STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING
3SK8 2011-07-06 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G apo protein at cryogenic temperature
3TP7 2011-09-21 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V99E at cryogenic temperature
3D4W 2009-01-06 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS A109R at cryogenic temperature
5KGU 2016-06-22 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS N118D at cryogenic temperature
2EXZ 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant T22C
2F0L 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant V23L/I72L
5I6W 2016-02-24 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS A58D at cryogenic temperature
2RBM 2008-10-07 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K at cryogenic temperature
1KAA 1994-01-31 1.9 STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE
5E1F 2015-10-14 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS G20E at cryogenic temperature
2F0O 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant V66I/I72V
4ZQ3 2015-05-27 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS I92T at cryogenic temperature
3NQT 2011-06-29 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A at cryogenic temperature
3EJI 2008-11-04 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K at cryogenic temperature
2EYH 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant T62S
2F0G 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant V66I
3QOL 2011-03-16 1.9 Crystal structure of Staphylococcal nuclease variant D+PHS/V23E at pH 6 determined at 100 K
2F0K 2006-10-24 1.9 Crystal structure of Staphylococcal nuclease mutant V23I/L25I
3R3O 2011-03-30 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature and with high redundancy
5I6Y 2016-02-24 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS A90E at cryogenic temperature
1NUC 1997-06-16 1.9 STAPHYLOCOCCAL NUCLEASE, V23C VARIANT
4KJO 2013-05-15 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A/V66T/V99T at cryogenic temperature
5CC4 2015-07-15 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66E at cryogenic temperature
6OK9 2019-05-08 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS K133M at cryogenic temperature
1EZ6 2000-10-18 1.9 STRUCTURE OF S. NUCLEASE STABILIZING SEXTUPLE MUTANT T33V/T41I/S59A/P117G/H124L/S128A
3C1E 2008-11-04 1.9 Crystal structure of Staphylococcal nuclease variant Delta+PHS L125K at cryogenic temperature
1KDB 1995-02-27 1.9 STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING
3NUC 1998-03-18 1.9 STAPHLOCOCCAL NUCLEASE, 1-N-PROPANE THIOL DISULFIDE TO V23C VARIANT
2OF1 2008-03-25 1.92 Cryogenic crystal structure of the Staphylococcal Nuclease variant truncated Delta+PHS I92W
1EQV 2003-07-08 1.94 SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
5C3X 2015-07-01 1.95 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D/N100E at cryogenic temperature
1ENC 1994-05-31 1.95 CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING
6EEG 2018-08-29 1.95 Crystal structure of Staphylcoccal nuclease variant Delta+PHS T62E at cryogenic temperature
1SNP 1997-02-12 1.95 PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE
5I9O 2016-03-23 1.95 Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K L103K at cryogenic temperature
5CV6 2015-08-12 1.95 Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92E at cryogenic temperature
2F0E 2006-10-24 1.95 Crystal structure of Staphylococcal nuclease mutant V23L
2F0Q 2006-10-24 1.95 Crystal structure of Staphylococcal nuclease mutant V66L/I92L
4KTA 2013-06-12 1.95 Crystal structure of Staphylococcal nuclease variant Delta+PHS R105Q at cryogenic temperatures
4EVO 2012-05-23 1.95 Crystal structure of Staphylococcal nuclease variant NVIAGA/E122G at cryogenic temperature
3U9O 2011-11-23 1.95 Crystal structure of Staphylococcal nuclease variant NVIAGA/N100G at cryogenic temperature
1A2T 1998-04-29 1.96 STAPHYLOCOCCAL NUCLEASE, B-MERCAPTOETHANOL DISULFIDE TO V23C VARIANT
2GX6 2007-03-20 1.97 Rational stabilization of E. coli ribose binding protein
2SNM 1993-01-15 1.97 IN A STAPHYLOCOCCAL NUCLEASE MUTANT THE SIDE-CHAIN of A LYSINE REPLACING VALINE 66 IS FULLY BURIED IN THE HYDROPHOBIC CORE
3D8G 2009-01-06 1.99 Crystal structure of Staphylococcal nuclease variant Delta+PHS I72R at cryogenic temperature
2OXP 2007-03-20 2.0 Crystal Structure of Staphylococcal Nuclease mutant V66D/P117G/H124L/S128A
1EYA 2000-10-18 2.0 STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T33V/T41I/P117G/H124L/S128A
3HZX 2010-05-19 2.0 Crystal Structure of Staphylococcal nuclease variant D+PHS/V66K at pH 9 determined at 100 K
1F2Y 2003-09-16 2.0 SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
1TQO 2004-07-06 2.0 Cryogenic Crystal Structure of Staphylococcal Nuclease Variant truncated Delta+PHS I92E
3C1F 2008-11-04 2.0 Crystal structure of Staphylococcal nuclease variant Delta+PHS V104K at cryogenic temperature
1A2U 1998-04-29 2.0 STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 1-N-BUTANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE
3E5S 2008-11-04 2.0 Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature
1STB 1994-07-31 2.0 ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE
3D6C 2008-11-04 2.0 Crystal structure of Staphylococcal nuclease variant PHS L38E at cryogenic temperature
2OEO 2008-03-25 2.0 Cryogenic crystal structure of Staphylococcal Nuclease variant truncated Delta+PHS I92D
1KDC 1995-02-27 2.0 STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING
1DRK 1995-01-26 2.0 PROBING PROTEIN-PROTEIN INTERACTIONS: THE RIBOSE-BINDING PROTEIN IN BACTERIAL TRANSPORT AND CHEMOTAXIS
3EVQ 2008-11-04 2.0 Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E at cryogenic temperature
2EY5 2006-10-24 2.0 Crystal structure of Staphylococcal nuclease mutant T41S
4K8J 2013-05-01 2.0 Crystal Structure of Staphylococcal nuclease mutant V23L/V66I
3NHH 2010-06-30 2.0 Crystal structure of Staphylococcal nuclease variant V23E-L36K at cryogenic temperature
2NUC 1998-03-18 2.0 STAPHLOCOCCAL NUCLEASE, ETHANE THIOL DISULFIDE TO V23C VARIANT
1F2M 2003-09-16 2.0 SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
2F0M 2006-10-24 2.0 Crystal structure of Staphylococcal nuclease mutant V23L/I72V
4E6I 2012-04-18 2.0 Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature
2F0V 2006-10-24 2.0 Crystal structure of Staphylococcal nuclease mutant V23L/V66L/I72L
2RKS 2008-04-15 2.01 Crystal structure of Staphylococcal nuclease variant PHS L38K at cryogenic temperature
2RDF 2008-06-10 2.01 Crystal Structure of staphyloccocal nuclease VIAGAN/E75A variant at cryogenic temperature
2ENB 1994-05-31 2.05 CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING
2F0P 2006-10-24 2.05 Crystal structure of Staphylococcal nuclease mutant V66I/V99I
1A3U 1998-04-29 2.05 STAPHYLOCOCCAL NUCLEASE, CYCLOHEXANE THIOL DISULFIDE TO V23C VARIANT
3D4D 2008-11-04 2.1 Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature
5NUC 1998-04-29 2.1 STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT
1AEX 1997-06-16 2.1 STAPHYLOCOCCAL NUCLEASE, METHANE THIOL DISULFIDE TO V23C VARIANT
5EKK 2015-11-18 2.1 Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D/L125E at cryogenic temperature
5EKL 2015-11-18 2.1 Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D/N100E at cryogenic temperature
1TR5 2004-07-06 2.1 Room temperature structure of Staphylococcal nuclease variant truncated Delta+PHS I92E
2F0W 2006-10-24 2.1 Crystal structure of Staphylococcal nuclease mutant V23I/L25I/V66L/I72L
1BA2 1998-07-15 2.1 D67R MUTANT OF D-RIBOSE-BINDING PROTEIN FROM ESCHERICHIA COLI
1A3T 1998-04-29 2.1 STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 2-FLUOROETHANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE
4K8I 2013-05-01 2.1 Crystal Structure of Staphylococcal Nuclease mutant I92V/V99L
1U9R 2004-12-21 2.1 Crystal Structure of Staphylococcal Nuclease mutant V66E/P117G/H124L/S128A at Room Temperature
3ERQ 2008-11-04 2.1 Crystal structure of Staphylococcal nuclease variant L25K at cryogenic temperature
2F0H 2006-10-24 2.15 Crystal structure of Staphylococcal nuclease mutant V66L
2F0F 2006-10-24 2.15 Crystal structure of Staphylococcal nuclease mutant L25I
1ENA 1994-05-31 2.15 CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING
3DHQ 2009-01-06 2.15 Crystal structure of Staphylococcal nuclease variant Delta+PHS A90R at cryogenic temperature
2QDB 2008-07-01 2.2 Crystal structure of staphylococcal nuclease variant E75Q/D21N/T33V/T41I/S59A/P117G/S128A at 100 K
1A3V 1998-04-29 2.2 STAPHYLOCOCCAL NUCLEASE, CYCLOPENTANE THIOL DISULFIDE TO V23C VARIANT
1DBP 1994-05-31 2.2 IDENTICAL MUTATIONS AT CORRESPONDING POSITIONS IN TWO HOMOLOGOUS PROTEINS WITH NON-IDENTICAL EFFECTS
6EEK 2018-08-29 2.2 Crystal structure of apo Staphylcoccal nuclease variant Delta+PHS T62E/V66K, pH 7 at cryogenic temperature
1URP 1998-06-17 2.3 D-RIBOSE-BINDING PROTEIN FROM ESCHERICHIA COLI
1DRJ 1995-01-26 2.5 PROBING PROTEIN-PROTEIN INTERACTIONS: THE RIBOSE-BINDING PROTEIN IN BACTERIAL TRANSPORT AND CHEMOTAXIS
2F0D 2006-10-24 2.5 Crystal structure of Staphylococcal nuclease mutant I92V
1NSN 1995-09-15 2.8 THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION
2GSI 2006-05-16 2.81 Crystal Structure of a Murine Fab in Complex with an 11 Residue Peptide Derived from Staphylococcal Nuclease

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.3 Thermonuclease Q6GIK1 NUC_STAAR
99.3 Thermonuclease Q8NXI6 NUC_STAAW
99.3 Thermonuclease Q6GB41 NUC_STAAS
99.1 Thermonuclease Q7A6P2 NUC_STAAN
99.1 Thermonuclease Q99VJ0 NUC_STAAM
99.3 Thermonuclease Q5HHM4 NUC_STAAC
100.0 Thermonuclease P00644 NUC_STAAU
97.3 Ribose import binding protein RbsB P0A2C5 RBSB_SALTY
97.3 Ribose import binding protein RbsB P0A2C6 RBSB_SALTI
100.0 Ribose import binding protein RbsB P02925 RBSB_ECOLI