CspA is a small protein that binds single-stranded RNA and DNA. The binding site of CspA consists of a cluster of aromatic amino acids, which form an unusually large nonpolar patch on the surface of the protein. Because nonpolar residues are generally found in the interiors of proteins, this cluster may have evolved to bind nucleic acids at the expense of protein stability. Study holds ProTherm entries: 3612, 3613, 3614, 3615, 3616, 3617, 3618, 3619, 3620, 3621, 3622, 3623, 3624, 3625, 3626, 3627 Extra Details: beta sheet; protein folding; aromatic interactions;,ssDNA and ssRNA binding
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:22 p.m.
|Number of data points||62|
|Proteins||Cold shock protein CspA ; Cold shock protein CspA|
|Assays/Quantities/Protocols||Experimental Assay: Cm temp:10.0 C ; Experimental Assay: m temp:10.0 C ; Experimental Assay: dG_H2O temp:10.0 C ; Experimental Assay: Cm temp:25.0 C ; Experimental Assay: m temp:25.0 C ; Experimental Assay: dG_H2O temp:25.0 C ; Derived Quantity: ddG_H2O temp:10.0 C ; Derived Quantity: ddG_H2O temp:25.0 C|
|Libraries||Mutations for sequence SGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKSLDEGQKVSFTIESGAKGPAAGNVTSL|