Stabilization of actin by phalloidin: a differential scanning calorimetric study.


Abstract

We have used differential scanning calorimetry to study the effects of phalloidin on F and G actin stability. For F actin, saturating concentrations of phalloidin induced an important shift on the transition temperature, Tm, from 69.5 degrees C to 83.5 degrees C. However, the calorimetric enthalpy remained unchanged. Using lower phalloidin concentrations, monomers linked to phalloidin, as well as neighboring unlinked monomers, were both stabilized. Contrary to previous reports, phalloidin was also shown to affect G actin, shifting its Tm from 59.5 degrees C to 75 degrees C. Two mechanisms are proposed to explain this finding: first, it could indicate a real interaction of phalloidin with G actin, and second, heating of the specimen during the scan could have induced polymerization of some G actin to the F form. The resulting F polymer would then interact with phalloidin, thus shifting the equilibrium between G and F actin towards the polymeric form. Study holds ProTherm entries: 7165 Extra Details: phalloidin; calorimetric enthalpy; polymerization;,polymeric form

Submission Details

ID: i9VahX5i

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:33 p.m.

Version: 1

Publication Details
Le Bihan T;Gicquaud C,Biochem. Biophys. Res. Commun. (1991) Stabilization of actin by phalloidin: a differential scanning calorimetric study. PMID:1755835
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
2W49 2010-05-05 ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE
2W4U 2010-08-25 Isometrically contracting insect asynchronous flight muscle quick frozen after a length step
3B5U 2008-04-15 Actin filament model from extended form of acromsomal bundle in the Limulus sperm
4B1Y 2013-07-31 1.29 Structure of the Phactr1 RPEL-3 bound to G-actin
2FXU 2006-03-07 1.35 X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution.
4K41 2014-10-01 1.4 Crystal structure of actin in complex with marine macrolide kabiramide C
1QZ5 2003-11-11 1.45 Structure of rabbit actin in complex with kabiramide C
2Q0U 2007-07-17 1.45 Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin
1WUA 2006-02-14 1.45 The structure of Aplyronine A-actin complex
2V52 2008-11-25 1.45 Structure of MAL-RPEL2 complexed to G-actin
2PBD 2007-11-13 1.5 Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*
3MN5 2010-06-02 1.5 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
5ZZA 2018-10-10 1.53 OdinProfilin/Rabbit Actin Complex
1J6Z 2001-08-15 1.54 UNCOMPLEXED ACTIN
3CIP 2008-08-19 1.6 Complex of Dictyostelium Discoideum Actin with Gelsolin
1QZ6 2003-11-11 1.6 Structure of rabbit actin in complex with jaspisamide A
1S22 2004-02-17 1.6 Absolute Stereochemistry of Ulapualide A
2ASM 2005-10-11 1.6 Structure of Rabbit Actin In Complex With Reidispongiolide A
2ASP 2005-10-11 1.64 Structure of Rabbit Actin In Complex With Reidispongiolide C
6MBK 2018-12-19 1.69 SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, First P212121 Crystal Form
3CI5 2008-08-19 1.7 Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin
2ASO 2005-10-11 1.7 Structure of Rabbit Actin In Complex With Sphinxolide B
2Q0R 2007-07-17 1.7 Structure of Pectenotoxin-2 Bound to Actin
1D4X 2001-05-02 1.75 Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin Segment 1 at 1.75 A resolution.
4H03 2013-02-20 1.75 Crystal structure of NAD+-Ia-actin complex
3SJH 2012-01-25 1.75 Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP-Latrunculin A
4B1V 2012-11-07 1.75 Structure of the Phactr1 RPEL-N domain bound to G-actin
6MBJ 2018-12-19 1.78 SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, P21 Crystal Form
4PKG 2014-07-30 1.8 Complex of ATP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
2HF4 2006-08-29 1.8 Crystal structure of Monomeric Actin in its ATP-bound state
1NM1 2003-01-21 1.8 Crystal Structure of D. Dicsoideum Actin Complexed With Gelsolin Segment 1 and Mg ATP at 1.8 A Resolution
3EKS 2008-10-07 1.8 Crystal Structure of Monomeric Actin bound to Cytochalasin D
2PAV 2007-10-23 1.8 Ternary complex of Profilin-Actin with the Last Poly-Pro of Human VASP
3W3D 2013-01-30 1.8 Crystal structure of smooth muscle G actin DNase I complex
1NLV 2003-01-21 1.8 Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human Gelsolin Segment 1
2A40 2005-11-01 1.8 Ternary complex of the WH2 domain of WAVE with Actin-DNAse I
2HF3 2006-08-29 1.8 Crystal structure of monomeric Actin in the ADP bound state
4B1X 2013-07-31 1.8 Structure of the Phactr1 RPEL-2 bound to G-actin
5YEE 2018-09-19 1.81 Crystal structure of LokiProfilin1/Rabbit Actin Complex
1NWK 2003-10-14 1.85 CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE
2A42 2005-11-01 1.85 Actin-DNAse I Complex
1NMD 2003-02-04 1.9 Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP
2GWJ 2006-08-29 1.9 SpvB ADP-ribosylated actin: hexagonal crystal form
2HMP 2006-09-19 1.9 Uncomplexed actin cleaved with protease ECP32
4H0Y 2013-02-20 1.94 Crystal structure of NAD+-Ia(E380S)-actin complex
4B1W 2013-07-31 1.95 Structure of the Phactr1 RPEL-2 domain bound to actin
6ICT 2019-02-27 1.95 Structure of SETD3 bound to SAH and methylated actin
6CXJ 2018-10-10 11.0 Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 2
6CXI 2018-10-10 11.0 Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 1
5NOG 2017-07-19 11.0 Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - 'Blocked' state
5NOJ 2017-08-02 11.0 Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - 'OPEN' state
3J8K 2015-01-14 12.0 Tilted state of actin, T2
3J8J 2015-01-14 12.0 Tilted state of actin, T1
3BYH 2008-02-19 12.0 Model of actin-fimbrin ABD2 complex
3LUE 2010-04-28 15.0 Model of alpha-actinin CH1 bound to F-actin
3MN9 2010-05-26 2.0 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
3DNI 1994-01-31 2.0 CRYSTALLOGRAPHIC REFINEMENT AND STRUCTURE OF DNASE I AT 2 ANGSTROMS RESOLUTION
3M6G 2010-09-08 2.0 Crystal structure of actin in complex with lobophorolide
2DNJ 1994-01-31 2.0 DNASE I-INDUCED DNA CONFORMATION. 2 ANGSTROMS STRUCTURE OF A DNASE I-OCTAMER COMPLEX
3U8X 2012-01-25 2.0 Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP
4PL8 2014-10-22 2.0 Structure of rabbit skeletal muscle actin in complex with a hybrid peptide comprising thymosin beta4 and the lysine-rich region of Cordon-Bleu
1ESV 2000-07-19 2.0 COMPLEX BETWEEN LATRUNCULIN A:RABBIT MUSCLE ALPHA ACTIN:HUMAN GELSOLIN DOMAIN 1
2FF3 2006-03-21 2.0 Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin
2GWK 2006-08-29 2.0 SpvB ADP-ribosylated actin: orthorhombic crystal form
1T44 2004-09-07 2.0 Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation
3MN7 2010-05-26 2.0 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
4B1U 2013-07-31 2.0 Structure of the Phactr1 RPEL domain and RPEL motif directed assemblies with G-actin reveal the molecular basis for actin binding cooperativity.
5YPU 2018-09-26 2.0 Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu MET72NLE WH2-motif peptide
1C0G 2000-03-01 2.0 CRYSTAL STRUCTURE OF 1:1 COMPLEX BETWEEN GELSOLIN SEGMENT 1 AND A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 228: Q228K/T229A/A230Y/E360H)
3MN6 2010-06-02 2.0 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
1YXQ 2005-05-17 2.01 Crystal structure of actin in complex with swinholide A
4H0V 2013-02-20 2.03 Crystal structure of NAD+-Ia(E378S)-actin complex
2FF6 2006-03-21 2.05 Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin
2A3Z 2005-11-01 2.08 Ternary complex of the WH2 domain of WASP with Actin-DNAse I
3U9Z 2012-01-25 2.09 Crystal structure between actin and a protein construct containing the first beta-thymosin domain of drosophila ciboulot (residues 2-58) with the three mutations N26D/Q27K/D28S
1KXP 2002-06-19 2.1 CRYSTAL STRUCTURE OF HUMAN VITAMIN D-BINDING PROTEIN IN COMPLEX WITH SKELETAL ACTIN
6ICV 2019-02-27 2.15 Structure of SETD3 bound to SAH and unmodified actin
4PKH 2014-07-30 2.15 Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
3UB5 2012-04-25 2.2 Profilin:actin with a wide open nucleotide cleft
6MBL 2018-12-19 2.2 SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, Second P212121 Crystal Form
6MGO 2018-11-21 2.2 Structure of rabbit actin in complex with Mycalolide B
1MDU 2003-01-07 2.2 Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1 (GS-1)
4H0T 2013-02-20 2.2 Crystal structure of Ia-ADPR-actin complex
3D2U 2008-07-08 2.21 Structure of UL18, a Peptide-Binding Viral MHC Mimic, Bound to a Host Inhibitory Receptor
3CHW 2008-08-19 2.3 Complex of Dictyostelium discoideum Actin with Profilin and the Last Poly-Pro of Human VASP
1EQY 2000-05-03 2.3 COMPLEX BETWEEN RABBIT MUSCLE ALPHA-ACTIN: HUMAN GELSOLIN DOMAIN 1
4PKI 2014-07-30 2.3 Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin
5ZZB 2018-10-10 2.3 LokiProfilin2/Rabbit Actin Complex
1RDW 2003-12-16 2.3 Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer
1DNK 1994-01-31 2.3 THE X-RAY STRUCTURE OF THE DNASE I-D(GGTATACC)2 COMPLEX AT 2.3 ANGSTROMS RESOLUTION
4H0X 2013-02-20 2.33 Crystal structure of NAD+-Ia(E380A)-actin complex
2VYP 2009-02-24 2.35 Rabbit-muscle G-actin in complex with myxobacterial rhizopodin
2V51 2008-11-25 2.35 Structure of MAL-RPEL1 complexed to actin
3A5N 2010-10-27 2.36 Crystal Structure of a Dictyostelium P109A Ca2+-Actin in Complex with Human Gelsolin Segment 1
5UBO 2017-12-20 2.39 Mical-oxidized Actin complex with Gelsolin Segment 1
3U4L 2012-04-25 2.4 Cryocooled bovine profilin:actin crystal structure to 2.4 A
3A5L 2010-10-27 2.4 Crystal Structure of a Dictyostelium P109A Mg2+-Actin in Complex with Human Gelsolin Segment 1
3A5M 2010-10-27 2.4 Crystal Structure of a Dictyostelium P109I Mg2+-Actin in Complex with Human Gelsolin Segment 1
6QRI 2019-07-03 2.4 Structure of rabbit G-actin in complex with chivosazole A
4Z94 2015-10-21 2.4 Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2
1DEJ 2000-03-01 2.4 CRYSTAL STRUCTURE OF A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 646: Q228K/T229A/A230Y/A231K/S232E/E360H) IN COMPLEX WITH HUMAN GELSOLIN SEGMENT 1
3A5O 2010-10-27 2.4 Crystal Structure of a Dictyostelium P109I Ca2+-Actin in Complex with Human Gelsolin Segment 1
1MA9 2003-02-04 2.4 Crystal structure of the complex of human vitamin D binding protein and rabbit muscle actin
1C0F 2000-03-01 2.4 CRYSTAL STRUCTURE OF DICTYOSTELIUM CAATP-ACTIN IN COMPLEX WITH GELSOLIN SEGMENT 1
4EFH 2012-04-11 2.48 Acanthamoeba Actin complex with Spir domain D
2A5X 2005-08-23 2.49 Crystal Structure of a Cross-linked Actin Dimer
1SQK 2004-06-15 2.5 CRYSTAL STRUCTURE OF CIBOULOT IN COMPLEX WITH SKELETAL ACTIN
1LOT 2002-07-31 2.5 CRYSTAL STRUCTURE OF THE COMPLEX OF ACTIN WITH VITAMIN D-BINDING PROTEIN
3U9D 2012-01-25 2.5 Crystal Structure of a chimera containing the N-terminal domain (residues 8-24) of drosophila Ciboulot and the C-terminal domain (residues 13-44) of bovine Thymosin-beta4, bound to G-actin-ATP
2Q97 2007-10-16 2.5 Complex of mammalian actin with toxofilin from toxoplasma gondii
3EL2 2008-10-07 2.5 Crystal Structure of Monomeric Actin Bound to Ca-ATP
3EKU 2008-10-07 2.5 Crystal Structure of Monomeric Actin bound to Cytochalasin D
2D1K 2006-09-12 2.5 Ternary complex of the WH2 domain of mim with actin-dnase I
2Q36 2007-06-05 2.5 Actin Dimer Cross-linked between Residues 191 and 374 and complexed with Kabiramide C
3DAW 2008-07-29 2.55 Structure of the actin-depolymerizing factor homology domain in complex with actin
2BTF 1994-06-22 2.55 THE STRUCTURE OF CRYSTALLINE PROFILIN-BETA-ACTIN
1P8Z 2003-10-14 2.6 Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156
2A41 2005-11-01 2.6 Ternary complex of the WH2 Domain of WIP with Actin-DNAse I
6GVC 2019-03-27 2.6 Structure of ArhGAP12 bound to G-Actin
2OAN 2007-05-01 2.61 Structure of oxidized beta-actin
1HLU 1997-10-15 2.65 STRUCTURE OF BOVINE BETA-ACTIN-PROFILIN COMPLEX WITH ACTIN BOUND ATP PHOSPHATES SOLVENT ACCESSIBLE
2Q1N 2007-06-05 2.7 Actin Dimer Cross-linked Between Residues 41 and 374
3HBT 2010-05-05 2.7 The structure of native G-actin
2Q31 2007-06-05 2.7 Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48.
4GY2 2013-02-20 2.71 Crystal structure of apo-Ia-actin complex
4M63 2013-10-23 2.75 Crystal Structure of a Filament-Like Actin Trimer Bound to the Bacterial Effector VopL
3UE5 2012-02-15 2.76 ECP-cleaved Actin in complex with Spir domain D
6FM2 2018-05-16 2.8 CARP domain of mouse cyclase-associated protein 1 (CAP1) bound to ADP-actin
1ATN 1992-07-15 2.8 Atomic structure of the actin:DNASE I complex
3MMV 2010-06-02 2.8 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
3BUZ 2008-05-13 2.81 Crystal structure of ia-bTAD-actin complex
1IJJ 2002-04-15 2.85 THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION
3M1F 2010-09-15 2.89 Crosslinked complex of actin with first W domain of Vibrio parahaemolyticus VopL
4K43 2014-10-01 2.9 Crystal structure of actin in complex with synthetic AplC tail analogue GC04 [N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide]
4K42 2014-10-01 2.9 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate]
4JHD 2013-06-19 2.91 Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu
4RWT 2015-10-14 2.98 Structure of actin-Lmod complex
1H1V 2003-01-24 2.99 gelsolin G4-G6/actin complex
2Y83 2011-03-30 22.9 Actin filament pointed end
5MVA 2017-11-29 27.7 Structure of the thin filament at high calcium concentration
5MVY 2018-02-14 28.4 Thin Filament at low calcium concentration
3TU5 2012-09-26 3.0 Actin complex with Gelsolin Segment 1 fused to Cobl segment
5WFN 2017-08-30 3.0 Revised model of leiomodin 2-mediated actin regulation (alternate refinement of PDB 4RWT)
3FFK 2009-10-06 3.0 Crystal structure of human Gelsolin domains G1-G3 bound to Actin
1RGI 2004-07-27 3.0 Crystal structure of gelsolin domains G1-G3 bound to actin
1RFQ 2003-12-16 3.0 Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer
1Y64 2005-01-18 3.05 Bni1p Formin Homology 2 Domain complexed with ATP-actin
6DJN 2019-02-27 3.1 Cryo-EM structure of ADP-Pi-actin filaments
6DJM 2019-02-27 3.1 Cryo-EM structure of AMPPNP-actin filaments
2YJE 2011-07-06 3.1 Oligomeric assembly of actin bound to MRTF-A
6C1D 2018-01-31 3.2 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
2VCP 2008-11-04 3.2 Crystal structure of N-Wasp VC domain in complex with skeletal actin
3CJB 2008-03-25 3.21 Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1
2ZWH 2009-01-20 3.3 Model for the F-actin structure
6FHL 2018-06-13 3.3 Cryo-EM structure of F-actin in complex with ADP-Pi
4B1Z 2012-11-07 3.3 Structure of the Phactr1 RPEL domain bound to G-actin
5OOF 2018-06-13 3.4 Cryo-EM structure of F-actin in complex with ADP-BeFx
4EAH 2012-12-12 3.4 Crystal structure of the formin homology 2 domain of FMNL3 bound to actin
3TPQ 2011-10-12 3.45 Crystal structure of wild-type MAL RPEL domain in complex with five G-actins
4WYB 2015-08-19 3.49 Structure of the Bud6 flank domain in complex with actin
1LCU 2002-05-01 3.5 Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution
6F1T 2018-01-17 3.5 Cryo-EM structure of two dynein tail domains bound to dynactin and BICDR1
2YJF 2011-07-06 3.5 Oligomeric assembly of actin bound to MRTF-A
6D8C 2018-09-19 3.54 Cryo-EM structure of FLNaABD E254K bound to phalloidin-stabilized F-actin
6DJO 2019-02-27 3.6 Cryo-EM structure of ADP-actin filaments
5OOC 2018-06-13 3.6 Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP
5OOE 2018-06-13 3.6 Cryo-EM structure of F-actin in complex with AppNHp (AMPPNP)
5JLF 2016-06-15 3.6 Structure of the F-actin-tropomyosin complex (reprocessed)
5OOD 2018-06-13 3.7 Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP-Pi
3J8A 2014-12-10 3.7 Structure of the F-actin-tropomyosin complex
5YU8 2018-05-23 3.8 Cofilin decorated actin filament
6C1G 2018-01-31 3.8 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
6C1H 2018-01-31 3.9 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
6AVB 2018-01-17 3.9 CryoEM structure of Mical Oxidized Actin (Class 1)
3CJC 2008-03-25 3.9 Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with DNase I and Gelsolin-segment 1
5JLH 2016-06-15 3.9 Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution
6AV9 2018-01-17 3.9 CryoEM structure of Mical Oxidized Actin (Class 1)
5ADX 2015-12-30 4.0 CryoEM structure of dynactin complex at 4.0 angstrom resolution
5ONV 2018-06-13 4.1 Cryo-EM structure of F-actin in complex with ADP
6BNP 2018-01-10 4.6 CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state
6BNV 2018-01-10 4.6 CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine
3J8I 2015-01-14 4.7 Near-Atomic Resolution for One State of F-Actin
5H53 2017-01-18 5.2 The structure of rabbit skeletal muscle actomyosin rigor complex at 5.2 angstrom.
6BNQ 2018-01-10 5.5 CryoEM structure of Myosin VI-Actin complex in the ADP state
6BNW 2018-01-10 5.5 CryoEM structure of Myosin VI-Actin complex in the ADP state, backbone-averaged with side chains truncated to alanine
6BNO 2018-01-10 5.5 Structure of bare actin filament
6BIH 2018-09-19 6.0 The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State
3G37 2010-11-03 6.0 Cryo-EM structure of actin filament in the presence of phosphate
3MFP 2010-09-29 6.6 Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map
6F38 2018-01-17 6.7 Cryo-EM structure of two dynein tail domains bound to dynactin and HOOK3
6ANU 2017-11-22 7.0 Cryo-EM structure of F-actin complexed with the beta-III-spectrin actin-binding domain
3M3N 2010-07-28 7.0 Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains
6BNU 2018-01-10 7.5 Structure of bare actin filament, backbone-averaged with sidechains truncated to alanine
3JBJ 2015-11-04 7.6 Cryo-EM reconstruction of F-actin
3J82 2015-05-20 7.7 Electron cryo-microscopy of DNGR-1 in complex with F-actin
4A7F 2012-08-01 7.7 Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 3)
4A7H 2012-08-01 7.8 Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2)
4V0U 2015-03-25 7.88 The crystal structure of ternary PP1G-PPP1R15B and G-actin complex
1O1D 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1F 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1A 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1M8Q 2002-09-10 70.0 Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle
1O1C 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1B 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O18 2002-11-27 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O19 2002-11-27 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1E 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1MVW 2002-11-20 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1G 2002-12-11 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
3J4K 2013-09-25 8.0 Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state
5NOL 2017-07-19 8.0 Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - 'Closed' state
4A7L 2012-08-01 8.1 Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 1)
3JBK 2015-11-04 8.2 Cryo-EM reconstruction of the metavinculin-actin interface
6F3A 2018-01-17 8.2 Cryo-EM structure of a single dynein tail domain bound to dynactin and BICD2N
5AFU 2015-03-11 8.2 Cryo-EM structure of dynein tail-dynactin-BICD2N complex
3JBI 2015-11-04 8.5 MDFF model of the vinculin tail domain bound to F-actin
4A7N 2012-08-01 8.9 Structure of bare F-actin filaments obtained from the same sample as the Actin-Tropomyosin-Myosin Complex
3J0S 2011-12-21 9.0 Remodeling of actin filaments by ADF cofilin proteins
6G2T 2018-10-17 9.0 human cardiac myosin binding protein C C1 Ig-domain bound to native cardiac thin filament
5KG8 2016-09-07 9.1 Rigor myosin X co-complexed with an actin filament
3B63 2008-11-18 9.5 Actin filament model in the extended form of acromsomal bundle in the Limulus sperm

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
93.8 Actin, alpha skeletal muscle Q24733 ACT_DICVI
93.3 Actin, alpha skeletal muscle P85911 ACT1_PSEMZ
96.5 Actin, alpha skeletal muscle Q39596 ACT_OXYRB
91.8 Actin, alpha skeletal muscle P00544 FGR_FSVGR
100.0 Actin, alpha skeletal muscle P10994 ACTS_PLEWA
90.1 Actin, alpha skeletal muscle Q25379 ACT3_LYTPI
96.3 Actin, alpha skeletal muscle Q11212 ACT_SPOLI
93.6 Actin, alpha skeletal muscle Q25381 ACTM_LYTPI
93.8 Actin, alpha skeletal muscle P30170 ACT10_SOLTU
92.9 Actin, alpha skeletal muscle Q92193 ACT_CRAVI
91.7 Actin, alpha skeletal muscle Q92192 ACT_CALFI
91.9 Actin, alpha skeletal muscle Q03342 ACT3_ECHGR
90.5 Actin, alpha skeletal muscle P93375 ACT7_TOBAC
92.4 Actin, alpha skeletal muscle P18602 ACT3_ARTSX
90.6 Actin, alpha skeletal muscle Q55EU6 ACT23_DICDI
93.9 Actin, alpha skeletal muscle P84856 ACTB_CHLPG
90.7 Actin, alpha skeletal muscle P12431 ACTM_STRPU
90.9 Actin, alpha skeletal muscle Q00215 ACTC_STYPL
90.4 Actin, alpha skeletal muscle Q553U6 ACT22_DICDI
91.2 Actin, alpha skeletal muscle Q54GX7 ACT10_DICDI
90.9 Actin, alpha skeletal muscle P07830 ACT1_DICDI
90.3 A Actin, alpha skeletal muscle O65315 ACT_COLSC
91.7 Actin, alpha skeletal muscle P41112 ACT1_PODCA
91.7 Actin, alpha skeletal muscle P02576 ACTA_PHYPO
90.8 A Actin, alpha skeletal muscle P53457 ACT3_DIPDE
92.0 Actin, alpha skeletal muscle P53474 ACTE_STRPU
92.0 Actin, alpha skeletal muscle Q07903 ACTC_STRPU
91.5 Actin, alpha skeletal muscle P53465 ACT1_LYTPI
91.8 Actin, alpha skeletal muscle P53466 ACT2_LYTPI
92.0 Actin, alpha skeletal muscle P92176 ACT2_LUMTE
92.3 Actin, alpha skeletal muscle P18499 ACTF_STRPU
92.0 Actin, alpha skeletal muscle P53473 ACTB_STRPU
92.0 Actin, alpha skeletal muscle P41113 ACT3_PODCA
92.3 Actin, alpha skeletal muscle P10990 ACT1_MESFR
92.8 Actin, alpha skeletal muscle P02578 ACT1_ACACA
93.2 Actin, alpha skeletal muscle P53458 ACT5_DIPDE
92.0 Actin, alpha skeletal muscle P02574 ACT4_DROME
91.8 Actin, alpha skeletal muscle P53461 ACTC_HALRO
92.3 Actin, alpha skeletal muscle P69005 ACTD_STRPU
92.3 Actin, alpha skeletal muscle P69004 ACT2_MESFR
92.3 Actin, alpha skeletal muscle O17320 ACT_CRAGI
91.8 Actin, alpha skeletal muscle P41341 ACTY_LIMPO
92.3 Actin, alpha skeletal muscle P49128 ACT1_AEDAE
92.2 Actin, alpha skeletal muscle O17503 ACTC_BRALA
92.3 Actin, alpha skeletal muscle P18601 ACT2_ARTSX
93.0 Actin, alpha skeletal muscle P91754 ACT_LUMRU
92.3 Actin, alpha skeletal muscle P53472 ACTA_STRPU
92.6 Actin, alpha skeletal muscle P45887 ACT5_BACDO
92.6 Actin, alpha skeletal muscle P07837 ACT2_BOMMO
92.8 Actin, alpha skeletal muscle O16808 ACT_MAYDE
93.0 Actin, alpha skeletal muscle P84336 ACTB_CAMDR
92.0 Actin, alpha skeletal muscle P53470 ACT1_SCHMA
92.6 Actin, alpha skeletal muscle P83968 ACT6_DROSI
92.6 Actin, alpha skeletal muscle P83967 ACT6_DROME
92.2 Actin, alpha skeletal muscle P53456 ACT2_DIPDE
92.6 Actin, alpha skeletal muscle P83969 ACT1_BACDO
92.8 Actin, alpha skeletal muscle P12717 ACTM_PISOC
92.8 Actin, alpha skeletal muscle Q0PGG4 ACTB_BOSMU
92.3 Actin, alpha skeletal muscle P41339 ACTA_LIMPO
92.6 Actin, alpha skeletal muscle P07836 ACT1_BOMMO
92.3 Actin, alpha skeletal muscle P18600 ACT1_ARTSX
93.6 Actin, alpha skeletal muscle P17126 ACT_HYDVU
92.8 Actin, alpha skeletal muscle P53501 ACT3_DROME
92.5 Actin, alpha skeletal muscle P68555 ACT_TAESO
92.8 Actin, alpha skeletal muscle O18500 ACT2_SACKO
92.8 Actin, alpha skeletal muscle P69003 ACT1_HELTB
92.8 Actin, alpha skeletal muscle P69002 ACT1_HELER
92.5 Actin, alpha skeletal muscle P68556 ACT1_DIPDE
92.8 Actin, alpha skeletal muscle P49871 ACT_MANSE
93.0 Actin, alpha skeletal muscle P90689 ACT_BRUMA
92.6 Actin, alpha skeletal muscle P92179 ACTC_BIOGL
93.3 Actin, alpha skeletal muscle P63256 ACTG_ANSAN
93.1 Actin, alpha skeletal muscle P17304 ACTM_APLCA
92.8 Actin, alpha skeletal muscle P53471 ACT2_SCHMA
93.1 Actin, alpha skeletal muscle P45885 ACT2_BACDO
92.6 Actin, alpha skeletal muscle Q964D9 ACTC_PLATR
92.6 Actin, alpha skeletal muscle Q964E3 ACTC_BIOAL
93.3 Actin, alpha skeletal muscle P29751 ACTB_RABIT
93.0 Actin, alpha skeletal muscle Q7ZVI7 ACTB1_DANRE
92.8 Actin, alpha skeletal muscle P12716 ACTC_PISOC
93.0 Actin, alpha skeletal muscle P53485 ACTB2_TAKRU
92.8 Actin, alpha skeletal muscle Q964E0 ACTC_BIOTE
93.3 Actin, alpha skeletal muscle P15475 ACTB_XENBO
93.6 Actin, alpha skeletal muscle P53506 ACT8_XENLA
93.4 Actin, alpha skeletal muscle P10981 ACT5_DROME
93.3 Actin, alpha skeletal muscle Q91ZK5 ACTB_SIGHI
93.3 Actin, alpha skeletal muscle P83750 ACTB_CYPCA
93.3 Actin, alpha skeletal muscle P83751 ACTB_CTEID
93.3 Actin, alpha skeletal muscle Q7ZVF9 ACTB2_DANRE
93.4 Actin, alpha skeletal muscle P45886 ACT3_BACDO
93.3 Actin, alpha skeletal muscle P79818 ACTB_ORYLA
93.3 Actin, alpha skeletal muscle P48975 ACTB_CRIGR
93.3 Actin, alpha skeletal muscle P53478 ACT5_CHICK
93.6 Actin, alpha skeletal muscle Q93131 ACTC_BRAFL
93.4 Actin, alpha skeletal muscle Q964E2 ACTC_BIOPF
93.4 Actin, alpha skeletal muscle Q964E1 ACTC_BIOOB
93.6 Actin, alpha skeletal muscle Q6P378 ACTG_XENTR
93.6 Actin, alpha skeletal muscle Q5JAK2 ACTG_PELLE
93.6 Actin, alpha skeletal muscle P53505 ACT5_XENLA
93.1 Actin, alpha skeletal muscle O18499 ACT1_SACKO
93.6 Actin, alpha skeletal muscle P60707 ACTB_TRIVU
93.6 Actin, alpha skeletal muscle Q4L0Y2 ACTB_SPECI
93.6 Actin, alpha skeletal muscle P60713 ACTB_SHEEP
93.6 Actin, alpha skeletal muscle P60711 ACTB_RAT
93.6 Actin, alpha skeletal muscle Q5R6G0 ACTB_PONAB
93.6 Actin, alpha skeletal muscle Q6QAQ1 ACTB_PIG
93.6 Actin, alpha skeletal muscle Q5R1X3 ACTB_PANTR
93.6 Actin, alpha skeletal muscle P60710 ACTB_MOUSE
93.6 Actin, alpha skeletal muscle Q711N9 ACTB_MESAU
93.6 Actin, alpha skeletal muscle Q4R561 ACTB_MACFA
93.6 Actin, alpha skeletal muscle P60709 ACTB_HUMAN
93.6 Actin, alpha skeletal muscle P60708 ACTB_HORSE
93.6 Actin, alpha skeletal muscle Q76N69 ACTB_CHLAE
93.6 Actin, alpha skeletal muscle P60706 ACTB_CHICK
93.6 Actin, alpha skeletal muscle Q71FK5 ACTB_CAVPO
93.6 Actin, alpha skeletal muscle O18840 ACTB_CANLF
93.6 Actin, alpha skeletal muscle P60712 ACTB_BOVIN
93.9 Actin, alpha skeletal muscle Q93129 ACTC_BRABE
93.6 Actin, alpha skeletal muscle P04829 ACT3_BOMMO
93.1 Actin, alpha skeletal muscle P92182 ACT1_LUMTE
93.9 Actin, alpha skeletal muscle A2BDB0 ACTG_XENLA
93.9 Actin, alpha skeletal muscle P63257 ACTG_TRIVU
93.9 Actin, alpha skeletal muscle P63259 ACTG_RAT
93.9 Actin, alpha skeletal muscle P63260 ACTG_MOUSE
93.9 Actin, alpha skeletal muscle P63261 ACTG_HUMAN
93.9 Actin, alpha skeletal muscle Q5ZMQ2 ACTG_CHICK
93.9 Actin, alpha skeletal muscle P63258 ACTG_BOVIN
93.9 Actin, alpha skeletal muscle Q8JJB8 ACTG_TRISC
93.9 Actin, alpha skeletal muscle O42161 ACTB_SALSA
93.9 Actin, alpha skeletal muscle Q6NVA9 ACTB_XENTR
94.1 Actin, alpha skeletal muscle P68143 ACTB_OREMO
93.9 Actin, alpha skeletal muscle P53486 ACTB3_TAKRU
94.1 Actin, alpha skeletal muscle P68142 ACTB1_TAKRU
93.9 Actin, alpha skeletal muscle Q25010 ACT3A_HELAM
94.1 Actin, alpha skeletal muscle O93400 ACTB_XENLA
93.9 Actin, alpha skeletal muscle P30162 ACT1_ONCVO
94.2 Actin, alpha skeletal muscle P53463 ACTM_HELER
93.9 Actin, alpha skeletal muscle P84185 ACT5C_ANOGA
93.9 Actin, alpha skeletal muscle P84183 ACT4_BOMMO
93.9 Actin, alpha skeletal muscle P84184 ACT3B_HELAM
93.6 Actin, alpha skeletal muscle P02572 ACT2_DROME
93.9 Actin, alpha skeletal muscle P10987 ACT1_DROME
93.9 Actin, alpha skeletal muscle P18603 ACT4_ARTSX
94.2 Actin, alpha skeletal muscle P53464 ACTM_HELTB
94.2 Actin, alpha skeletal muscle P41340 ACT3_LIMPO
95.2 Actin, alpha skeletal muscle Q00214 ACTM_STYPL
93.9 Actin, alpha skeletal muscle P0DM42 ACT3_CAEEL
94.2 Actin, alpha skeletal muscle P10984 ACT2_CAEEL
93.9 Actin, alpha skeletal muscle P0DM41 ACT1_CAEEL
94.2 Actin, alpha skeletal muscle P10986 ACT4_CAEEL
94.7 Actin, alpha skeletal muscle P30163 ACT2_ONCVO
94.7 Actin, alpha skeletal muscle Q26065 ACT_PLAMG
94.9 Actin, alpha skeletal muscle P53467 ACTM_MOLOC
96.0 Actin, alpha skeletal muscle P26198 ACTM_STYCL
96.3 Actin, alpha skeletal muscle P53475 ACTN_STYCL
96.8 Actin, alpha skeletal muscle P53460 ACT1_HALRO
97.1 Actin, alpha skeletal muscle O15998 ACTM_CIOSA
97.1 Actin, alpha skeletal muscle P27130 ACT2_HALRO
97.1 Actin, alpha skeletal muscle Q25472 ACT2_MOLOC
98.4 A Actin, alpha skeletal muscle P63269 ACTH_RAT
98.4 A Actin, alpha skeletal muscle P63268 ACTH_MOUSE
98.4 A Actin, alpha skeletal muscle P63267 ACTH_HUMAN
98.4 A Actin, alpha skeletal muscle P63270 ACTH_CHICK
98.4 A Actin, alpha skeletal muscle Q5E9B5 ACTH_BOVIN
97.6 Actin, alpha skeletal muscle P08023 ACTA_CHICK
97.9 Actin, alpha skeletal muscle P62738 ACTA_RAT
97.9 Actin, alpha skeletal muscle P62740 ACTA_RABIT
97.9 Actin, alpha skeletal muscle P62737 ACTA_MOUSE
97.9 Actin, alpha skeletal muscle P62736 ACTA_HUMAN
97.9 Actin, alpha skeletal muscle P62739 ACTA_BOVIN
98.4 Actin, alpha skeletal muscle Q6P8G3 ACT3_XENTR
98.1 Actin, alpha skeletal muscle P49055 ACTS_CARAU
98.1 Actin, alpha skeletal muscle Q98972 ACTS_ORYLA
98.9 Actin, alpha skeletal muscle P04752 ACT3_XENLA
98.4 Actin, alpha skeletal muscle P68264 ACTS_OREMO
98.4 Actin, alpha skeletal muscle P68140 ACTSA_TAKRU
98.7 Actin, alpha skeletal muscle P04751 ACTC_XENLA
98.7 Actin, alpha skeletal muscle P53482 ACTSB_TAKRU
98.7 Actin, alpha skeletal muscle P53479 ACTS_CYPCA
98.9 Actin, alpha skeletal muscle P53480 ACTC_TAKRU
98.9 Actin, alpha skeletal muscle Q6P640 ACTC_XENTR
98.9 Actin, alpha skeletal muscle P68035 ACTC_RAT
98.9 Actin, alpha skeletal muscle P68033 ACTC_MOUSE
98.9 Actin, alpha skeletal muscle P68032 ACTC_HUMAN
98.9 Actin, alpha skeletal muscle P68034 ACTC_CHICK
98.9 Actin, alpha skeletal muscle Q3ZC07 ACTC_BOVIN
99.2 Actin, alpha skeletal muscle P10995 ACT2_XENLA
99.2 Actin, alpha skeletal muscle P20399 ACT2_XENTR
99.7 Actin, alpha skeletal muscle Q90X97 ACTS_ATRMM
100.0 Actin, alpha skeletal muscle P68136 ACTS_RAT
100.0 Actin, alpha skeletal muscle P68135 ACTS_RABIT
100.0 Actin, alpha skeletal muscle Q5R9Q5 ACTS_PONAB
100.0 Actin, alpha skeletal muscle P68137 ACTS_PIG
100.0 Actin, alpha skeletal muscle P68134 ACTS_MOUSE
100.0 Actin, alpha skeletal muscle P68133 ACTS_HUMAN
100.0 Actin, alpha skeletal muscle P68139 ACTS_CHICK
100.0 Actin, alpha skeletal muscle P68138 ACTS_BOVIN
93.1 D Actin, alpha skeletal muscle P11937 DNAS1_SHEEP
99.2 D Actin, alpha skeletal muscle P00639 DNAS1_BOVIN