Kinetic study of the thermal denaturation of G actin using differential scanning calorimetry and intrinsic fluorescence spectroscopy.

Abstract

Previous studies of the thermal denaturation of G actin have yielded conflicting results which have led to contradictory interpretations of the denaturation process. In their interpretations, these authors postulated that the thermal denaturation of G actin can be described by thermodynamical equilibrium laws despite the fact that this transition is irreversible. Using differential scanning calorimetry and fluorescence spectroscopy, we now show that thermal denaturation of G actin does not obey to equilibrium thermodynamics, but rather, is a two-step irreversible phenomenon under kinetic control. It can therefore be analyzed using the recently developed Sanchez-Ruiz equations. Study holds ProTherm entries: 10024 Extra Details: additive : sodium azide(0.02 %), thermodynamical equilibrium;equilibrium thermodynamics;two-step irreversible,phenomenon;kinetic control

Submission Details

ID: hnKqTszm

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:39 p.m.

Version: 1

Publication Details
Le Bihan T;Gicquaud C,Biochem. Biophys. Res. Commun. (1993) Kinetic study of the thermal denaturation of G actin using differential scanning calorimetry and intrinsic fluorescence spectroscopy. PMID:8352763
Additional Information

Study Summary

Number of data points 2
Proteins Actin, alpha skeletal muscle ; Actin, alpha skeletal muscle
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dHcal ; Experimental Assay: Tm
Libraries Mutations for sequence A:DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHR/D:LKIAAFNIRTFGETKMSNATLASYIVRIVRRYDIVLIQEVRDSHLVAVGKLLDYLNQDDPNTYHYVVSEPLGRNSYKERYLFLFRPNKVSVLDTYQYDDGCGNCGNDSFSREPAVVKFSSHSTKVKEFAIVALHSAPSDAVAEINSLYDVYLDVQQKWHLNDVMLMGDFNADCSYVTSSQWSSIRLRTSSTFQWLIPDSADTTATSTNCAYDRIVVAGSLLQSSVVPGSAAPFDFQAAYGLSNEMALAISDHYPVEVTLT

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Actin, alpha skeletal muscle P68138 ACTS_BOVIN
100.0 Actin, alpha skeletal muscle P68139 ACTS_CHICK
100.0 Actin, alpha skeletal muscle P68133 ACTS_HUMAN
100.0 Actin, alpha skeletal muscle P68134 ACTS_MOUSE
100.0 Actin, alpha skeletal muscle P68137 ACTS_PIG
100.0 Actin, alpha skeletal muscle Q5R9Q5 ACTS_PONAB
100.0 Actin, alpha skeletal muscle P68135 ACTS_RABIT
100.0 Actin, alpha skeletal muscle P68136 ACTS_RAT
99.7 Actin, alpha skeletal muscle Q90X97 ACTS_ATRMM
99.2 Actin, alpha skeletal muscle P20399 ACT2_XENTR
99.2 Actin, alpha skeletal muscle P10995 ACT2_XENLA
98.9 Actin, alpha skeletal muscle Q3ZC07 ACTC_BOVIN
98.9 Actin, alpha skeletal muscle P68034 ACTC_CHICK
98.9 Actin, alpha skeletal muscle P68032 ACTC_HUMAN
98.9 Actin, alpha skeletal muscle P68033 ACTC_MOUSE
98.9 Actin, alpha skeletal muscle P68035 ACTC_RAT
98.9 Actin, alpha skeletal muscle Q6P640 ACTC_XENTR
98.9 Actin, alpha skeletal muscle P53480 ACTC_TAKRU
98.7 Actin, alpha skeletal muscle P53479 ACTS_CYPCA
98.7 Actin, alpha skeletal muscle P53482 ACTSB_TAKRU
98.7 Actin, alpha skeletal muscle P04751 ACTC_XENLA
98.4 Actin, alpha skeletal muscle P68140 ACTSA_TAKRU
98.4 Actin, alpha skeletal muscle P68264 ACTS_OREMO
98.9 Actin, alpha skeletal muscle P04752 ACT3_XENLA
98.1 Actin, alpha skeletal muscle Q98972 ACTS_ORYLA
98.1 Actin, alpha skeletal muscle P49055 ACTS_CARAU
98.4 Actin, alpha skeletal muscle Q6P8G3 ACT3_XENTR
97.9 Actin, alpha skeletal muscle P62739 ACTA_BOVIN
97.9 Actin, alpha skeletal muscle P62736 ACTA_HUMAN
97.9 Actin, alpha skeletal muscle P62737 ACTA_MOUSE
97.9 Actin, alpha skeletal muscle P62740 ACTA_RABIT
97.9 Actin, alpha skeletal muscle P62738 ACTA_RAT
97.6 Actin, alpha skeletal muscle P08023 ACTA_CHICK
98.4 A Actin, alpha skeletal muscle Q5E9B5 ACTH_BOVIN
98.4 A Actin, alpha skeletal muscle P63270 ACTH_CHICK
98.4 A Actin, alpha skeletal muscle P63267 ACTH_HUMAN
98.4 A Actin, alpha skeletal muscle P63268 ACTH_MOUSE
98.4 A Actin, alpha skeletal muscle P63269 ACTH_RAT
97.1 Actin, alpha skeletal muscle Q25472 ACT2_MOLOC
97.1 Actin, alpha skeletal muscle P27130 ACT2_HALRO
97.1 Actin, alpha skeletal muscle O15998 ACTM_CIOSA
96.8 Actin, alpha skeletal muscle P53460 ACT1_HALRO
96.3 Actin, alpha skeletal muscle P53475 ACTN_STYCL
96.0 Actin, alpha skeletal muscle P26198 ACTM_STYCL
94.9 Actin, alpha skeletal muscle P53467 ACTM_MOLOC
94.7 Actin, alpha skeletal muscle Q26065 ACT_PLAMG
94.7 Actin, alpha skeletal muscle P30163 ACT2_ONCVO
94.2 Actin, alpha skeletal muscle P10986 ACT4_CAEEL
93.9 Actin, alpha skeletal muscle P0DM41 ACT1_CAEEL
94.2 Actin, alpha skeletal muscle P10984 ACT2_CAEEL
93.9 Actin, alpha skeletal muscle P0DM42 ACT3_CAEEL
95.2 Actin, alpha skeletal muscle Q00214 ACTM_STYPL
94.2 Actin, alpha skeletal muscle P41340 ACT3_LIMPO
94.2 Actin, alpha skeletal muscle P53464 ACTM_HELTB
93.9 Actin, alpha skeletal muscle P18603 ACT4_ARTSX
93.9 Actin, alpha skeletal muscle P10987 ACT1_DROME
93.6 Actin, alpha skeletal muscle P02572 ACT2_DROME
93.9 Actin, alpha skeletal muscle P84184 ACT3B_HELAM
93.9 Actin, alpha skeletal muscle P84183 ACT4_BOMMO
93.9 Actin, alpha skeletal muscle P84185 ACT5C_ANOGA
94.2 Actin, alpha skeletal muscle P53463 ACTM_HELER
93.9 Actin, alpha skeletal muscle P30162 ACT1_ONCVO
94.1 Actin, alpha skeletal muscle O93400 ACTB_XENLA
93.9 Actin, alpha skeletal muscle Q25010 ACT3A_HELAM
94.1 Actin, alpha skeletal muscle P68142 ACTB1_TAKRU
93.9 Actin, alpha skeletal muscle P53486 ACTB3_TAKRU
94.1 Actin, alpha skeletal muscle P68143 ACTB_OREMO
93.9 Actin, alpha skeletal muscle Q6NVA9 ACTB_XENTR
93.9 Actin, alpha skeletal muscle O42161 ACTB_SALSA
93.9 Actin, alpha skeletal muscle Q8JJB8 ACTG_TRISC
93.9 Actin, alpha skeletal muscle P63258 ACTG_BOVIN
93.9 Actin, alpha skeletal muscle Q5ZMQ2 ACTG_CHICK
93.9 Actin, alpha skeletal muscle P63261 ACTG_HUMAN
93.9 Actin, alpha skeletal muscle P63260 ACTG_MOUSE
93.9 Actin, alpha skeletal muscle P63259 ACTG_RAT
93.9 Actin, alpha skeletal muscle P63257 ACTG_TRIVU
93.9 Actin, alpha skeletal muscle A2BDB0 ACTG_XENLA
93.1 Actin, alpha skeletal muscle P92182 ACT1_LUMTE
93.6 Actin, alpha skeletal muscle P04829 ACT3_BOMMO
93.9 Actin, alpha skeletal muscle Q93129 ACTC_BRABE
93.6 Actin, alpha skeletal muscle P60712 ACTB_BOVIN
93.6 Actin, alpha skeletal muscle O18840 ACTB_CANLF
93.6 Actin, alpha skeletal muscle Q71FK5 ACTB_CAVPO
93.6 Actin, alpha skeletal muscle P60706 ACTB_CHICK
93.6 Actin, alpha skeletal muscle Q76N69 ACTB_CHLAE
93.6 Actin, alpha skeletal muscle P60708 ACTB_HORSE
93.6 Actin, alpha skeletal muscle P60709 ACTB_HUMAN
93.6 Actin, alpha skeletal muscle Q4R561 ACTB_MACFA
93.6 Actin, alpha skeletal muscle Q711N9 ACTB_MESAU
93.6 Actin, alpha skeletal muscle P60710 ACTB_MOUSE
93.6 Actin, alpha skeletal muscle Q5R1X3 ACTB_PANTR
93.6 Actin, alpha skeletal muscle Q6QAQ1 ACTB_PIG
93.6 Actin, alpha skeletal muscle Q5R6G0 ACTB_PONAB
93.6 Actin, alpha skeletal muscle P60711 ACTB_RAT
93.6 Actin, alpha skeletal muscle P60713 ACTB_SHEEP
93.6 Actin, alpha skeletal muscle Q4L0Y2 ACTB_SPECI
93.6 Actin, alpha skeletal muscle P60707 ACTB_TRIVU
93.1 Actin, alpha skeletal muscle O18499 ACT1_SACKO
93.6 Actin, alpha skeletal muscle P53505 ACT5_XENLA
93.6 Actin, alpha skeletal muscle Q5JAK2 ACTG_PELLE
93.6 Actin, alpha skeletal muscle Q6P378 ACTG_XENTR
93.4 Actin, alpha skeletal muscle Q964E1 ACTC_BIOOB
93.4 Actin, alpha skeletal muscle Q964E2 ACTC_BIOPF
93.6 Actin, alpha skeletal muscle Q93131 ACTC_BRAFL
93.3 Actin, alpha skeletal muscle P53478 ACT5_CHICK
93.3 Actin, alpha skeletal muscle P48975 ACTB_CRIGR
93.3 Actin, alpha skeletal muscle P79818 ACTB_ORYLA
93.4 Actin, alpha skeletal muscle P45886 ACT3_BACDO
93.3 Actin, alpha skeletal muscle Q7ZVF9 ACTB2_DANRE
93.3 Actin, alpha skeletal muscle P83751 ACTB_CTEID
93.3 Actin, alpha skeletal muscle P83750 ACTB_CYPCA
93.3 Actin, alpha skeletal muscle Q91ZK5 ACTB_SIGHI
93.4 Actin, alpha skeletal muscle P10981 ACT5_DROME
93.6 Actin, alpha skeletal muscle P53506 ACT8_XENLA
93.3 Actin, alpha skeletal muscle P15475 ACTB_XENBO
92.8 Actin, alpha skeletal muscle Q964E0 ACTC_BIOTE
93.0 Actin, alpha skeletal muscle P53485 ACTB2_TAKRU
92.8 Actin, alpha skeletal muscle P12716 ACTC_PISOC
93.0 Actin, alpha skeletal muscle Q7ZVI7 ACTB1_DANRE
93.3 Actin, alpha skeletal muscle P29751 ACTB_RABIT
92.6 Actin, alpha skeletal muscle Q964E3 ACTC_BIOAL
92.6 Actin, alpha skeletal muscle Q964D9 ACTC_PLATR
93.1 Actin, alpha skeletal muscle P45885 ACT2_BACDO
92.8 Actin, alpha skeletal muscle P53471 ACT2_SCHMA
93.1 Actin, alpha skeletal muscle P17304 ACTM_APLCA
93.3 Actin, alpha skeletal muscle P63256 ACTG_ANSAN
92.6 Actin, alpha skeletal muscle P92179 ACTC_BIOGL
93.0 Actin, alpha skeletal muscle P90689 ACT_BRUMA
92.8 Actin, alpha skeletal muscle P49871 ACT_MANSE
92.5 Actin, alpha skeletal muscle P68556 ACT1_DIPDE
92.8 Actin, alpha skeletal muscle P69002 ACT1_HELER
92.8 Actin, alpha skeletal muscle P69003 ACT1_HELTB
92.8 Actin, alpha skeletal muscle O18500 ACT2_SACKO
92.5 Actin, alpha skeletal muscle P68555 ACT_TAESO
92.8 Actin, alpha skeletal muscle P53501 ACT3_DROME
93.6 Actin, alpha skeletal muscle P17126 ACT_HYDVU
92.3 Actin, alpha skeletal muscle P18600 ACT1_ARTSX
92.6 Actin, alpha skeletal muscle P07836 ACT1_BOMMO
92.3 Actin, alpha skeletal muscle P41339 ACTA_LIMPO
92.8 Actin, alpha skeletal muscle Q0PGG4 ACTB_BOSMU
92.8 Actin, alpha skeletal muscle P12717 ACTM_PISOC
92.6 Actin, alpha skeletal muscle P83969 ACT1_BACDO
92.2 Actin, alpha skeletal muscle P53456 ACT2_DIPDE
92.6 Actin, alpha skeletal muscle P83967 ACT6_DROME
92.6 Actin, alpha skeletal muscle P83968 ACT6_DROSI
92.0 Actin, alpha skeletal muscle P53470 ACT1_SCHMA
93.0 Actin, alpha skeletal muscle P84336 ACTB_CAMDR
92.8 Actin, alpha skeletal muscle O16808 ACT_MAYDE
92.6 Actin, alpha skeletal muscle P07837 ACT2_BOMMO
92.6 Actin, alpha skeletal muscle P45887 ACT5_BACDO
92.3 Actin, alpha skeletal muscle P53472 ACTA_STRPU
93.0 Actin, alpha skeletal muscle P91754 ACT_LUMRU
92.3 Actin, alpha skeletal muscle P18601 ACT2_ARTSX
92.2 Actin, alpha skeletal muscle O17503 ACTC_BRALA
92.3 Actin, alpha skeletal muscle P49128 ACT1_AEDAE
91.8 Actin, alpha skeletal muscle P41341 ACTY_LIMPO
92.3 Actin, alpha skeletal muscle O17320 ACT_CRAGI
92.3 Actin, alpha skeletal muscle P69004 ACT2_MESFR
92.3 Actin, alpha skeletal muscle P69005 ACTD_STRPU
91.8 Actin, alpha skeletal muscle P53461 ACTC_HALRO
92.0 Actin, alpha skeletal muscle P02574 ACT4_DROME
93.2 Actin, alpha skeletal muscle P53458 ACT5_DIPDE
92.8 Actin, alpha skeletal muscle P02578 ACT1_ACACA
92.3 Actin, alpha skeletal muscle P10990 ACT1_MESFR
92.0 Actin, alpha skeletal muscle P41113 ACT3_PODCA
92.0 Actin, alpha skeletal muscle P53473 ACTB_STRPU
92.3 Actin, alpha skeletal muscle P18499 ACTF_STRPU
92.0 Actin, alpha skeletal muscle P92176 ACT2_LUMTE
91.8 Actin, alpha skeletal muscle P53466 ACT2_LYTPI
91.5 Actin, alpha skeletal muscle P53465 ACT1_LYTPI
92.0 Actin, alpha skeletal muscle Q07903 ACTC_STRPU
92.0 Actin, alpha skeletal muscle P53474 ACTE_STRPU
90.8 A Actin, alpha skeletal muscle P53457 ACT3_DIPDE
91.7 Actin, alpha skeletal muscle P02576 ACTA_PHYPO
91.7 Actin, alpha skeletal muscle P41112 ACT1_PODCA
90.3 A Actin, alpha skeletal muscle O65315 ACT_COLSC
90.9 Actin, alpha skeletal muscle P07830 ACT1_DICDI
91.2 Actin, alpha skeletal muscle Q54GX7 ACT10_DICDI
90.4 Actin, alpha skeletal muscle Q553U6 ACT22_DICDI
90.9 Actin, alpha skeletal muscle Q00215 ACTC_STYPL
90.7 Actin, alpha skeletal muscle P12431 ACTM_STRPU
93.9 Actin, alpha skeletal muscle P84856 ACTB_CHLPG
90.6 Actin, alpha skeletal muscle Q55EU6 ACT23_DICDI
92.4 Actin, alpha skeletal muscle P18602 ACT3_ARTSX
90.5 Actin, alpha skeletal muscle P93375 ACT7_TOBAC
91.9 Actin, alpha skeletal muscle Q03342 ACT3_ECHGR
91.7 Actin, alpha skeletal muscle Q92192 ACT_CALFI
92.9 Actin, alpha skeletal muscle Q92193 ACT_CRAVI
93.8 Actin, alpha skeletal muscle P30170 ACT10_SOLTU
93.6 Actin, alpha skeletal muscle Q25381 ACTM_LYTPI
96.3 Actin, alpha skeletal muscle Q11212 ACT_SPOLI
90.1 Actin, alpha skeletal muscle Q25379 ACT3_LYTPI
100.0 Actin, alpha skeletal muscle P10994 ACTS_PLEWA
91.8 Actin, alpha skeletal muscle P00544 FGR_FSVGR
96.5 Actin, alpha skeletal muscle Q39596 ACT_OXYRB
93.3 Actin, alpha skeletal muscle P85911 ACT1_PSEMZ
93.8 Actin, alpha skeletal muscle Q24733 ACT_DICVI
99.2 D Actin, alpha skeletal muscle P00639 DNAS1_BOVIN
93.1 D Actin, alpha skeletal muscle P11937 DNAS1_SHEEP