Kinetic study of the thermal denaturation of G actin using differential scanning calorimetry and intrinsic fluorescence spectroscopy.


Abstract

Previous studies of the thermal denaturation of G actin have yielded conflicting results which have led to contradictory interpretations of the denaturation process. In their interpretations, these authors postulated that the thermal denaturation of G actin can be described by thermodynamical equilibrium laws despite the fact that this transition is irreversible. Using differential scanning calorimetry and fluorescence spectroscopy, we now show that thermal denaturation of G actin does not obey to equilibrium thermodynamics, but rather, is a two-step irreversible phenomenon under kinetic control. It can therefore be analyzed using the recently developed Sanchez-Ruiz equations. Study holds ProTherm entries: 10024 Extra Details: additive : sodium azide(0.02 %), thermodynamical equilibrium;equilibrium thermodynamics;two-step irreversible,phenomenon;kinetic control

Submission Details

ID: hnKqTszm

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:39 p.m.

Version: 1

Publication Details
Le Bihan T;Gicquaud C,Biochem. Biophys. Res. Commun. (1993) Kinetic study of the thermal denaturation of G actin using differential scanning calorimetry and intrinsic fluorescence spectroscopy. PMID:8352763
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3U9D 2011-10-18T00:00:00+0000 2.5 Crystal Structure of a chimera containing the N-terminal domain (residues 8-24) of drosophila Ciboulot and the C-terminal domain (residues 13-44) of bovine Thymosin-beta4, bound to G-actin-ATP
3W3D 2012-12-20T00:00:00+0000 1.8 Crystal structure of smooth muscle G actin DNase I complex
6JAT 2019-01-25T00:00:00+0000 2.71 Crystal structure of SETD3 bound to Actin peptide and SFG
5NOL 2017-04-12T00:00:00+0000 8.0 Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - "Closed" state
5NOG 2017-04-12T00:00:00+0000 11.0 Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - "Blocked" state
5NOJ 2017-04-12T00:00:00+0000 11.0 Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - "OPEN" state
6KLL 2019-07-30T00:00:00+0000 3.0 F-actin of cardiac thin filament in low-calcium state
6KLN 2019-07-30T00:00:00+0000 3.4 F-actin of cardiac thin filament in high-calcium state
7AQK 2020-10-22T00:00:00+0000 9.0 Model of the actin filament Arp2/3 complex branch junction in cells
7NEP 2021-02-04T00:00:00+0000 10.2 Homology model of the in situ actomyosin complex from the A-band of mouse psoas muscle sarcomere in the rigor state

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
92.3 Actin, alpha skeletal muscle P53472 ACTA_STRPU
92.6 Actin, alpha skeletal muscle P45887 ACT5_BACDO
92.6 Actin, alpha skeletal muscle P07837 ACT2_BOMMO
92.8 Actin, alpha skeletal muscle O16808 ACT_MAYDE
93.0 Actin, alpha skeletal muscle P84336 ACTB_CAMDR
92.0 Actin, alpha skeletal muscle P53470 ACT1_SCHMA
92.6 Actin, alpha skeletal muscle P83968 ACT6_DROSI
92.6 Actin, alpha skeletal muscle P83967 ACT6_DROME
92.2 Actin, alpha skeletal muscle P53456 ACT2_DIPDE
92.6 Actin, alpha skeletal muscle P83969 ACT1_BACDO
92.8 Actin, alpha skeletal muscle P12717 ACTM_PISOC
92.8 Actin, alpha skeletal muscle Q0PGG4 ACTB_BOSMU
92.3 Actin, alpha skeletal muscle P41339 ACTA_LIMPO
92.6 Actin, alpha skeletal muscle P07836 ACT1_BOMMO
92.3 Actin, alpha skeletal muscle P18600 ACT1_ARTSX
93.6 Actin, alpha skeletal muscle P17126 ACT_HYDVU
92.8 Actin, alpha skeletal muscle P53501 ACT3_DROME
92.5 Actin, alpha skeletal muscle P68555 ACT_TAESO
92.8 Actin, alpha skeletal muscle O18500 ACT2_SACKO
92.8 Actin, alpha skeletal muscle P69003 ACT1_HELTB
92.8 Actin, alpha skeletal muscle P69002 ACT1_HELER
92.5 Actin, alpha skeletal muscle P68556 ACT1_DIPDE
92.8 Actin, alpha skeletal muscle P49871 ACT_MANSE
93.0 Actin, alpha skeletal muscle P90689 ACT_BRUMA
92.6 Actin, alpha skeletal muscle P92179 ACTC_BIOGL
93.3 Actin, alpha skeletal muscle P63256 ACTG_ANSAN
93.1 Actin, alpha skeletal muscle P17304 ACTM_APLCA
92.8 Actin, alpha skeletal muscle P53471 ACT2_SCHMA
93.1 Actin, alpha skeletal muscle P45885 ACT2_BACDO
92.6 Actin, alpha skeletal muscle Q964D9 ACTC_PLATR
92.6 Actin, alpha skeletal muscle Q964E3 ACTC_BIOAL
93.3 Actin, alpha skeletal muscle P29751 ACTB_RABIT
93.0 Actin, alpha skeletal muscle Q7ZVI7 ACTB1_DANRE
92.8 Actin, alpha skeletal muscle P12716 ACTC_PISOC
93.0 Actin, alpha skeletal muscle P53485 ACTB2_TAKRU
92.8 Actin, alpha skeletal muscle Q964E0 ACTC_BIOTE
93.3 Actin, alpha skeletal muscle P15475 ACTB_XENBO
93.6 Actin, alpha skeletal muscle P53506 ACT8_XENLA
93.4 Actin, alpha skeletal muscle P10981 ACT5_DROME
93.3 Actin, alpha skeletal muscle Q91ZK5 ACTB_SIGHI
93.3 Actin, alpha skeletal muscle P83750 ACTB_CYPCA
93.3 Actin, alpha skeletal muscle P83751 ACTB_CTEID
93.3 Actin, alpha skeletal muscle Q7ZVF9 ACTB2_DANRE
93.4 Actin, alpha skeletal muscle P45886 ACT3_BACDO
93.3 Actin, alpha skeletal muscle P79818 ACTB_ORYLA
93.3 Actin, alpha skeletal muscle P48975 ACTB_CRIGR
93.3 Actin, alpha skeletal muscle P53478 ACT5_CHICK
93.6 Actin, alpha skeletal muscle Q93131 ACTC_BRAFL
93.4 Actin, alpha skeletal muscle Q964E2 ACTC_BIOPF
93.4 Actin, alpha skeletal muscle Q964E1 ACTC_BIOOB
93.6 Actin, alpha skeletal muscle Q6P378 ACTG_XENTR
93.6 Actin, alpha skeletal muscle Q5JAK2 ACTG_PELLE
93.6 Actin, alpha skeletal muscle P53505 ACT5_XENLA
93.1 Actin, alpha skeletal muscle O18499 ACT1_SACKO
93.6 Actin, alpha skeletal muscle P60707 ACTB_TRIVU
93.6 Actin, alpha skeletal muscle Q4L0Y2 ACTB_SPECI
93.6 Actin, alpha skeletal muscle P60713 ACTB_SHEEP
93.6 Actin, alpha skeletal muscle P60711 ACTB_RAT
93.6 Actin, alpha skeletal muscle Q5R6G0 ACTB_PONAB
93.6 Actin, alpha skeletal muscle Q6QAQ1 ACTB_PIG
93.6 Actin, alpha skeletal muscle Q5R1X3 ACTB_PANTR
93.6 Actin, alpha skeletal muscle P60710 ACTB_MOUSE
93.6 Actin, alpha skeletal muscle Q711N9 ACTB_MESAU
93.6 Actin, alpha skeletal muscle Q4R561 ACTB_MACFA
93.6 Actin, alpha skeletal muscle P60709 ACTB_HUMAN
93.6 Actin, alpha skeletal muscle P60708 ACTB_HORSE
93.6 Actin, alpha skeletal muscle Q76N69 ACTB_CHLAE
93.6 Actin, alpha skeletal muscle P60706 ACTB_CHICK
93.6 Actin, alpha skeletal muscle Q71FK5 ACTB_CAVPO
93.6 Actin, alpha skeletal muscle O18840 ACTB_CANLF
93.6 Actin, alpha skeletal muscle P60712 ACTB_BOVIN
93.9 Actin, alpha skeletal muscle Q93129 ACTC_BRABE
93.6 Actin, alpha skeletal muscle P04829 ACT3_BOMMO
93.1 Actin, alpha skeletal muscle P92182 ACT1_LUMTE
93.9 Actin, alpha skeletal muscle A2BDB0 ACTG_XENLA
93.9 Actin, alpha skeletal muscle P63257 ACTG_TRIVU
93.9 Actin, alpha skeletal muscle P63259 ACTG_RAT
93.9 Actin, alpha skeletal muscle P63260 ACTG_MOUSE
93.9 Actin, alpha skeletal muscle P63261 ACTG_HUMAN
93.9 Actin, alpha skeletal muscle Q5ZMQ2 ACTG_CHICK
93.9 Actin, alpha skeletal muscle P63258 ACTG_BOVIN
93.9 Actin, alpha skeletal muscle Q8JJB8 ACTG_TRISC
93.9 Actin, alpha skeletal muscle O42161 ACTB_SALSA
93.9 Actin, alpha skeletal muscle Q6NVA9 ACTB_XENTR
94.1 Actin, alpha skeletal muscle P68143 ACTB_OREMO
93.9 Actin, alpha skeletal muscle P53486 ACTB3_TAKRU
94.1 Actin, alpha skeletal muscle P68142 ACTB1_TAKRU
93.9 Actin, alpha skeletal muscle Q25010 ACT3A_HELAM
94.1 Actin, alpha skeletal muscle O93400 ACTB_XENLA
93.9 Actin, alpha skeletal muscle P30162 ACT1_ONCVO
94.2 Actin, alpha skeletal muscle P53463 ACTM_HELER
93.9 Actin, alpha skeletal muscle P84185 ACT5C_ANOGA
93.9 Actin, alpha skeletal muscle P84183 ACT4_BOMMO
93.9 Actin, alpha skeletal muscle P84184 ACT3B_HELAM
93.6 Actin, alpha skeletal muscle P02572 ACT2_DROME
93.9 Actin, alpha skeletal muscle P10987 ACT1_DROME
93.9 Actin, alpha skeletal muscle P18603 ACT4_ARTSX
94.2 Actin, alpha skeletal muscle P53464 ACTM_HELTB
94.2 Actin, alpha skeletal muscle P41340 ACT3_LIMPO
95.2 Actin, alpha skeletal muscle Q00214 ACTM_STYPL
93.9 Actin, alpha skeletal muscle P0DM42 ACT3_CAEEL
94.2 Actin, alpha skeletal muscle P10984 ACT2_CAEEL
93.9 Actin, alpha skeletal muscle P0DM41 ACT1_CAEEL
94.2 Actin, alpha skeletal muscle P10986 ACT4_CAEEL
94.7 Actin, alpha skeletal muscle P30163 ACT2_ONCVO
94.7 Actin, alpha skeletal muscle Q26065 ACT_PLAMG
94.9 Actin, alpha skeletal muscle P53467 ACTM_MOLOC
96.0 Actin, alpha skeletal muscle P26198 ACTM_STYCL
96.3 Actin, alpha skeletal muscle P53475 ACTN_STYCL
96.8 Actin, alpha skeletal muscle P53460 ACT1_HALRO
97.1 Actin, alpha skeletal muscle O15998 ACTM_CIOSA
97.1 Actin, alpha skeletal muscle P27130 ACT2_HALRO
97.1 Actin, alpha skeletal muscle Q25472 ACT2_MOLOC
98.4 A Actin, alpha skeletal muscle P63269 ACTH_RAT
98.4 A Actin, alpha skeletal muscle P63268 ACTH_MOUSE
98.4 A Actin, alpha skeletal muscle P63267 ACTH_HUMAN
98.4 A Actin, alpha skeletal muscle P63270 ACTH_CHICK
98.4 A Actin, alpha skeletal muscle Q5E9B5 ACTH_BOVIN
97.6 Actin, alpha skeletal muscle P08023 ACTA_CHICK
97.9 Actin, alpha skeletal muscle P62738 ACTA_RAT
97.9 Actin, alpha skeletal muscle P62740 ACTA_RABIT
97.9 Actin, alpha skeletal muscle P62737 ACTA_MOUSE
97.9 Actin, alpha skeletal muscle P62736 ACTA_HUMAN
97.9 Actin, alpha skeletal muscle P62739 ACTA_BOVIN
98.4 Actin, alpha skeletal muscle Q6P8G3 ACT3_XENTR
98.1 Actin, alpha skeletal muscle P49055 ACTS_CARAU
98.1 Actin, alpha skeletal muscle Q98972 ACTS_ORYLA
98.9 Actin, alpha skeletal muscle P04752 ACT3_XENLA
98.4 Actin, alpha skeletal muscle P68264 ACTS_OREMO
98.4 Actin, alpha skeletal muscle P68140 ACTSA_TAKRU
98.7 Actin, alpha skeletal muscle P04751 ACTC_XENLA
98.7 Actin, alpha skeletal muscle P53482 ACTSB_TAKRU
98.7 Actin, alpha skeletal muscle P53479 ACTS_CYPCA
98.9 Actin, alpha skeletal muscle P53480 ACTC_TAKRU
98.9 Actin, alpha skeletal muscle Q6P640 ACTC_XENTR
98.9 Actin, alpha skeletal muscle P68035 ACTC_RAT
98.9 Actin, alpha skeletal muscle P68033 ACTC_MOUSE
98.9 Actin, alpha skeletal muscle P68032 ACTC_HUMAN
98.9 Actin, alpha skeletal muscle P68034 ACTC_CHICK
98.9 Actin, alpha skeletal muscle Q3ZC07 ACTC_BOVIN
99.2 Actin, alpha skeletal muscle P10995 ACT2_XENLA
99.2 Actin, alpha skeletal muscle P20399 ACT2_XENTR
99.7 Actin, alpha skeletal muscle Q90X97 ACTS_ATRMM
100.0 Actin, alpha skeletal muscle P68136 ACTS_RAT
93.8 Actin, alpha skeletal muscle Q24733 ACT_DICVI
93.3 Actin, alpha skeletal muscle P85911 ACT1_PSEMZ
96.5 Actin, alpha skeletal muscle Q39596 ACT_OXYRB
91.8 Actin, alpha skeletal muscle P00544 FGR_FSVGR
100.0 Actin, alpha skeletal muscle P10994 ACTS_PLEWA
90.1 Actin, alpha skeletal muscle Q25379 ACT3_LYTPI
96.3 Actin, alpha skeletal muscle Q11212 ACT_SPOLI
93.6 Actin, alpha skeletal muscle Q25381 ACTM_LYTPI
93.8 Actin, alpha skeletal muscle P30170 ACT10_SOLTU
92.9 Actin, alpha skeletal muscle Q92193 ACT_CRAVI
91.7 Actin, alpha skeletal muscle Q92192 ACT_CALFI
91.9 Actin, alpha skeletal muscle Q03342 ACT3_ECHGR
90.5 Actin, alpha skeletal muscle P93375 ACT7_TOBAC
92.4 Actin, alpha skeletal muscle P18602 ACT3_ARTSX
90.6 Actin, alpha skeletal muscle Q55EU6 ACT23_DICDI
93.9 Actin, alpha skeletal muscle P84856 ACTB_CHLPG
90.7 Actin, alpha skeletal muscle P12431 ACTM_STRPU
90.9 Actin, alpha skeletal muscle Q00215 ACTC_STYPL
90.4 Actin, alpha skeletal muscle Q553U6 ACT22_DICDI
91.2 Actin, alpha skeletal muscle Q54GX7 ACT10_DICDI
90.9 Actin, alpha skeletal muscle P07830 ACT1_DICDI
90.3 A Actin, alpha skeletal muscle O65315 ACT_COLSC
91.7 Actin, alpha skeletal muscle P41112 ACT1_PODCA
91.7 Actin, alpha skeletal muscle P02576 ACTA_PHYPO
90.8 A Actin, alpha skeletal muscle P53457 ACT3_DIPDE
92.0 Actin, alpha skeletal muscle P53474 ACTE_STRPU
92.0 Actin, alpha skeletal muscle Q07903 ACTC_STRPU
91.5 Actin, alpha skeletal muscle P53465 ACT1_LYTPI
91.8 Actin, alpha skeletal muscle P53466 ACT2_LYTPI
92.0 Actin, alpha skeletal muscle P92176 ACT2_LUMTE
92.3 Actin, alpha skeletal muscle P18499 ACTF_STRPU
92.0 Actin, alpha skeletal muscle P53473 ACTB_STRPU
92.0 Actin, alpha skeletal muscle P41113 ACT3_PODCA
92.3 Actin, alpha skeletal muscle P10990 ACT1_MESFR
92.8 Actin, alpha skeletal muscle P02578 ACT1_ACACA
93.2 Actin, alpha skeletal muscle P53458 ACT5_DIPDE
92.0 Actin, alpha skeletal muscle P02574 ACT4_DROME
91.8 Actin, alpha skeletal muscle P53461 ACTC_HALRO
92.3 Actin, alpha skeletal muscle P69005 ACTD_STRPU
92.3 Actin, alpha skeletal muscle P69004 ACT2_MESFR
92.3 Actin, alpha skeletal muscle O17320 ACT_CRAGI
91.8 Actin, alpha skeletal muscle P41341 ACTY_LIMPO
92.3 Actin, alpha skeletal muscle P49128 ACT1_AEDAE
92.2 Actin, alpha skeletal muscle O17503 ACTC_BRALA
92.3 Actin, alpha skeletal muscle P18601 ACT2_ARTSX
93.0 Actin, alpha skeletal muscle P91754 ACT_LUMRU
100.0 Actin, alpha skeletal muscle P68135 ACTS_RABIT
100.0 Actin, alpha skeletal muscle Q5R9Q5 ACTS_PONAB
100.0 Actin, alpha skeletal muscle P68137 ACTS_PIG
100.0 Actin, alpha skeletal muscle P68134 ACTS_MOUSE
100.0 Actin, alpha skeletal muscle P68133 ACTS_HUMAN
100.0 Actin, alpha skeletal muscle P68139 ACTS_CHICK
100.0 Actin, alpha skeletal muscle P68138 ACTS_BOVIN
93.1 D Actin, alpha skeletal muscle P11937 DNAS1_SHEEP
99.2 D Actin, alpha skeletal muscle P00639 DNAS1_BOVIN