Kinetic study of the thermal denaturation of G actin using differential scanning calorimetry and intrinsic fluorescence spectroscopy.


Abstract

Previous studies of the thermal denaturation of G actin have yielded conflicting results which have led to contradictory interpretations of the denaturation process. In their interpretations, these authors postulated that the thermal denaturation of G actin can be described by thermodynamical equilibrium laws despite the fact that this transition is irreversible. Using differential scanning calorimetry and fluorescence spectroscopy, we now show that thermal denaturation of G actin does not obey to equilibrium thermodynamics, but rather, is a two-step irreversible phenomenon under kinetic control. It can therefore be analyzed using the recently developed Sanchez-Ruiz equations. Study holds ProTherm entries: 10024 Extra Details: additive : sodium azide(0.02 %), thermodynamical equilibrium;equilibrium thermodynamics;two-step irreversible,phenomenon;kinetic control

Submission Details

ID: hnKqTszm

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:39 p.m.

Version: 1

Publication Details
Le Bihan T;Gicquaud C,Biochem. Biophys. Res. Commun. (1993) Kinetic study of the thermal denaturation of G actin using differential scanning calorimetry and intrinsic fluorescence spectroscopy. PMID:8352763
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