Thermodynamic analysis of the structural stability of the shiga toxin B-subunit.


Abstract

The conformational stability of Shiga toxin B-subunit (STxB), a pentameric protein from Shigella dysenteriae has been characterized by high sensitivity differential scanning calorimetry and circular dichroism spectroscopy under different solvent conditions. It is shown that the thermal folding/unfolding of STxB is a reversible process involving a highly cooperative transition between folded pentamer and unfolded monomers. The conformational stability of STxB is pH dependent and because of its pentameric nature is also concentration dependent. STxB is maximally stable in the pH range from 5 to 9 (Delta G upon unfolding is close to 13 kcal per mol of monomer at 25 degrees C), and its stability decreases both at lower and at higher pH values. The pH dependence of the Gibbs energy of stabilization between pH 2.5 and 5 is consistent with the change in the ionizable state of an average of four groups per monomer upon unfolding. Structural thermodynamic calculations show that the stabilization of the STxB pentamer is primarily due to the interactions established between monomers rather than intramonomer interactions. The folding of an isolated monomer into the conformation existing in the pentamer is unfavorable and expected to be characterized by a free-energy change upon folding in the order of 2.5 kcal mol(-1) at 25 degrees C. On the average, intersubunit interaction induced upon oligomerization of folded monomers should contribute close to -13.4 kcal per mol of monomer to bring the overall Gibbs energy to the experimentally determined value at this temperature. Study holds ProTherm entries: 16553, 16554, 16555, 16556, 16557, 16558, 16559, 16560, 16561, 16562, 16563, 16564, 16565, 16566, 16567, 16568, 16569, 16570, 16571, 16572, 16573, 16574 Extra Details: pentameric protein; cooperative transition; concentration dependent; intramonomer interactions

Submission Details

ID: gw24GH7M3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:48 p.m.

Version: 1

Publication Details
Pina DG;Gómez J;Villar E;Johannes L;Shnyrov VL,Biochemistry (2003) Thermodynamic analysis of the structural stability of the shiga toxin B-subunit. PMID:12899637
Additional Information

Number of data points 44
Proteins Shiga toxin subunit A ; Shiga toxin subunit B
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dCp buffers:Phosphate: 10 mM, prot_conc:34.7 microM ; Experimental Assay: dHvH prot_conc:34.7 microM ; Experimental Assay: dG buffers:Phosphate: 10 mM, prot_conc:34.7 microM ; Experimental Assay: dCp buffers:Phosphate: 10 mM, prot_conc:25.5 microM ; Experimental Assay: dHvH prot_conc:25.5 microM ; Experimental Assay: dG buffers:Phosphate: 10 mM, prot_conc:25.5 microM ; Experimental Assay: dCp buffers:Phosphate: 10 mM, prot_conc:18.6 microM ; Experimental Assay: dHvH prot_conc:18.6 microM ; Experimental Assay: dG buffers:Phosphate: 10 mM, prot_conc:18.6 microM ; Experimental Assay: dCp buffers:Phosphate: 10 mM, prot_conc:9.1 microM ; Experimental Assay: dHvH prot_conc:9.1 microM ; Experimental Assay: dG buffers:Phosphate: 10 mM, prot_conc:9.1 microM ; Experimental Assay: dCp buffers:Phosphate: 10 mM, prot_conc:4.2 microM ; Experimental Assay: dHvH prot_conc:4.2 microM ; Experimental Assay: dG buffers:Phosphate: 10 mM, prot_conc:4.2 microM ; Experimental Assay: dCp prot_conc:265.0 microM, buffers:HEPES: 10 mM ; Experimental Assay: dHcal prot_conc:265.0 microM ; Experimental Assay: dG prot_conc:265.0 microM, buffers:HEPES: 10 mM ; Experimental Assay: dCp prot_conc:237.1 microM, buffers:HEPES: 10 mM ; Experimental Assay: dHcal prot_conc:237.1 microM ; Experimental Assay: dG prot_conc:237.1 microM, buffers:HEPES: 10 mM ; Experimental Assay: dCp prot_conc:229.2 microM, buffers:HEPES: 10 mM ; Experimental Assay: dHcal prot_conc:229.2 microM ; Experimental Assay: dG prot_conc:229.2 microM, buffers:HEPES: 10 mM ; Experimental Assay: dCp prot_conc:115.1 microM, buffers:HEPES: 10 mM ; Experimental Assay: dHcal prot_conc:115.1 microM ; Experimental Assay: dG prot_conc:115.1 microM, buffers:HEPES: 10 mM ; Experimental Assay: dCp prot_conc:80.0 microM, buffers:HEPES: 10 mM ; Experimental Assay: dHcal prot_conc:80.0 microM ; Experimental Assay: dG prot_conc:80.0 microM, buffers:HEPES: 10 mM ; Experimental Assay: dCp prot_conc:71.4 microM, buffers:HEPES: 10 mM ; Experimental Assay: dHcal prot_conc:71.4 microM ; Experimental Assay: dG prot_conc:71.4 microM, buffers:HEPES: 10 mM ; Experimental Assay: Tm buffers:Phosphate: 10 mM, prot_conc:34.7 microM ; Experimental Assay: Tm buffers:Phosphate: 10 mM, prot_conc:25.5 microM ; Experimental Assay: Tm buffers:Phosphate: 10 mM, prot_conc:18.6 microM ; Experimental Assay: Tm buffers:Phosphate: 10 mM, prot_conc:9.1 microM ; Experimental Assay: Tm buffers:Phosphate: 10 mM, prot_conc:4.2 microM ; Experimental Assay: Tm prot_conc:265.0 microM, buffers:HEPES: 10 mM ; Experimental Assay: Tm prot_conc:237.1 microM, buffers:HEPES: 10 mM ; Experimental Assay: Tm prot_conc:229.2 microM, buffers:HEPES: 10 mM ; Experimental Assay: Tm prot_conc:115.1 microM, buffers:HEPES: 10 mM ; Experimental Assay: Tm prot_conc:80.0 microM, buffers:HEPES: 10 mM ; Experimental Assay: Tm prot_conc:71.4 microM, buffers:HEPES: 10 mM
Libraries Mutations for sequence A:KEFTLDFSTAKTYVDSLNVIRSAIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTGFVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTSYLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAEDVDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMASDEFPSMCPADGRVRGITHNKILWDSSTLGAILM/B:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/C:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/D:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/E:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/F:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/G:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/H:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/I:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/J:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/K:TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR/L:KEFTLDFSTAKTYVDSLNVIRSAIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTGFVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTSYLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAEDVDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMASDEFPSMCPADGRVRGITHNKILWDSSTLGAILM
Sequence Assay Result Units