Effect of foreign N-terminal residues on the conformational stability of human lysozyme.
Abstract
To minutely understand the effect of foreign N-terminal residues on the conformational stability of human lysozyme, five mutant proteins were constructed: two had Met or Ala in place of the N-terminal Lys residue (K1M and K1A, respectively), and others had one additional residue, Met, Gly or Pro, to the N-terminal Lys residue (Met(-1), Gly(-1) and Pro(-1), respectively). The thermodynamic parameters for denaturation of these mutant proteins were examined by differential scanning calorimetry and were compared with that of the wild-type protein. Three mutants with the extra residue were significantly destabilized: the changes in unfolding Gibbs energy (DeltaDeltaG) were -9.1 to -12.2 kJ.mol-1. However, the stability of two single substitutions at the N-terminal slightly decreased; the DeltaDeltaG values were only -0.5 to -2.5 kJ.mol-1. The results indicate that human lysozyme is destabilized by an expanded N-terminal residue. The crystal structural analyses of K1M, K1A and Gly(-1) revealed that the introduction of a residue at the N-terminal of human lysozyme caused the destruction of hydrogen bond networks with ordered water molecules, resulting in the destabilization of the protein. Study holds ProTherm entries: 9150, 9151, 9152, 9153, 9154, 9155, 9156, 9157, 9158, 9159, 9160, 9161, 14489, 14490 Extra Details: extra N-terminal residue; human lysozyme; protein stability;,mutant protein; crystal structure
Submission Details
ID: gmvLTXod
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:37 p.m.
Version: 1
Publication Details
Takano K;Tsuchimori K;Yamagata Y;Yutani K,Eur. J. Biochem. (1999) Effect of foreign N-terminal residues on the conformational stability of human lysozyme. PMID:10561612Additional Information
Study Summary
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
|---|
Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant PDB Entries
| Structure ID | Release Date | Resolution | Structure Title |
|---|---|---|---|
| 1IY4 | 2002-07-31 | Solution structure of the human lysozyme at 35 degree C | |
| 1IY3 | 2002-07-31 | Solution Structure of the Human lysozyme at 4 degree C | |
| 2NWD | 2006-12-19 | 1.04 | Structure of chemically synthesized human lysozyme at 1 Angstrom resolution |
| 5LSH | 2017-06-21 | 1.06 | human lysozyme in complex with a tetrasaccharide fragment of the O-chain of LPS from Klebsiella pneumoniae |
| 1JSF | 1998-04-29 | 1.15 | FULL-MATRIX LEAST-SQUARES REFINEMENT OF HUMAN LYSOZYME |
| 1JWR | 2001-09-19 | 1.4 | Crystal structure of human lysozyme at 100 K |
| 1IWW | 2002-09-04 | 1.4 | Crystal Structure Analysis of Human lysozyme at 152K. |
| 1IWV | 2002-09-04 | 1.4 | Crystal Structure Analysis of Human lysozyme at 147K. |
| 1IWU | 2002-09-04 | 1.4 | Crystal Structure Analysis of Human lysozyme at 127K. |
| 1IWY | 2002-09-04 | 1.4 | Crystal Structure Analysis of Human lysozyme at 170K. |
| 1IWT | 2002-09-04 | 1.4 | Crystal Structure Analysis of Human lysozyme at 113K. |
| 1IWX | 2002-09-04 | 1.4 | Crystal Structure Analysis of Human lysozyme at 161K. |
| 1IWZ | 2002-09-04 | 1.48 | Crystal Structure Analysis of Human lysozyme at 178K. |
| 1REX | 1997-02-12 | 1.5 | NATIVE HUMAN LYSOZYME |
| 1LZR | 1995-04-20 | 1.5 | STRUCTURAL CHANGES OF THE ACTIVE SITE CLEFT AND DIFFERENT SACCHARIDE BINDING MODES IN HUMAN LYSOZYME CO-CRYSTALLIZED WITH HEXA-N-ACETYL-CHITOHEXAOSE AT PH 4.0 |
| 1LZ1 | 1985-01-02 | 1.5 | REFINEMENT OF HUMAN LYSOZYME AT 1.5 ANGSTROMS RESOLUTION. ANALYSIS OF NON-BONDED AND HYDROGEN-BOND INTERACTIONS |
| 3FE0 | 2009-12-08 | 1.5 | X-ray crystal structure of wild type human lysozyme in D2O |
| 4R0P | 2014-12-17 | 1.52 | Ifqins, an amyloid forming segment from human lysozyme spanning residues 56-61 |
| 1LMT | 1995-03-31 | 1.6 | STRUCTURE OF A CONFORMATIONALLY CONSTRAINED ARG-GLY-ASP SEQUENCE INSERTED INTO HUMAN LYSOZYME |
| 1LZS | 1995-04-20 | 1.6 | STRUCTURAL CHANGES OF THE ACTIVE SITE CLEFT AND DIFFERENT SACCHARIDE BINDING MODES IN HUMAN LYSOZYME CO-CRYSTALLIZED WITH HEXA-N-ACETYL-CHITOHEXAOSE AT PH 4.0 |
| 1JKC | 1997-05-15 | 1.6 | HUMAN LYSOZYME MUTANT WITH TRP 109 REPLACED BY PHE |
| 1JKB | 1997-05-15 | 1.66 | HUMAN LYSOZYME MUTANT WITH GLU 35 REPLACED BY ALA |
| 1JKA | 1997-05-15 | 1.66 | HUMAN LYSOZYME MUTANT WITH GLU 35 REPLACED BY ASP |
| 1GE1 | 2000-11-08 | 1.7 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS |
| 1TAY | 1993-01-15 | 1.7 | DISSECTION OF THE FUNCTIONAL ROLE OF STRUCTURAL ELEMENTS OF TYROSINE-63 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME |
| 1REY | 1997-02-12 | 1.7 | HUMAN LYSOZYME-N,N'-DIACETYLCHITOBIOSE COMPLEX |
| 1REZ | 1997-02-12 | 1.7 | HUMAN LYSOZYME-N-ACETYLLACTOSAMINE COMPLEX |
| 1TCY | 1993-01-15 | 1.7 | DISSECTION OF THE FUNCTIONAL ROLE OF STRUCTURAL ELEMENTS OF TYROSINE-63 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME |
| 1TDY | 1993-01-15 | 1.7 | DISSECTION OF THE FUNCTIONAL ROLE OF STRUCTURAL ELEMENTS OF TYROSINE-63 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME |
| 1LAA | 1993-01-15 | 1.77 | X-RAY STRUCTURE OF GLU 53 HUMAN LYSOZYME |
| 1TBY | 1993-01-15 | 1.77 | DISSECTION OF THE FUNCTIONAL ROLE OF STRUCTURAL ELEMENTS OF TYROSINE-63 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME |
| 134L | 1993-10-31 | 1.77 | ROLE OF ARG 115 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME. X-RAY STRUCTURE OF HIS 115 AND GLU 115 MUTANTS |
| 133L | 1993-10-31 | 1.77 | ROLE OF ARG 115 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME. X-RAY STRUCTURE OF HIS 115 AND GLU 115 MUTANTS |
| 1GE3 | 2000-11-08 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS |
| 1WQQ | 1998-07-01 | 1.8 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1GFG | 2000-12-20 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 4ML7 | 2014-07-23 | 1.8 | Crystal structure of Brucella abortus PliC in complex with human lysozyme |
| 1B7L | 1999-01-27 | 1.8 | VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES |
| 1OUJ | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V99A MUTANT |
| 1LZ5 | 1993-10-31 | 1.8 | STRUCTURAL AND FUNCTIONAL ANALYSES OF THE ARG-GLY-ASP SEQUENCE INTRODUCED INTO HUMAN LYSOZYME |
| 1GB2 | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1YAM | 1996-04-03 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS |
| 1LHM | 1992-04-15 | 1.8 | THE CRYSTAL STRUCTURE OF A MUTANT LYSOZYME C77(SLASH)95A WITH INCREASED SECRETION EFFICIENCY IN YEAST |
| 1DI3 | 1999-12-08 | 1.8 | ROLE OF AMINO ACID RESIDUES AT TURNS IN THE CONFORMATIONAL STABILITY AND FOLDING OF HUMAN LYSOZYME |
| 1IP2 | 2001-11-14 | 1.8 | G48A HUMAN LYSOZYME |
| 1GB8 | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1LHL | 1994-01-31 | 1.8 | ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS |
| 2MEB | 1998-07-15 | 1.8 | CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS |
| 1EQE | 2000-04-19 | 1.8 | CRYSTAL STRUCTURES OF SALT BRIDGE MUTANTS OF HUMAN LYSOZYME |
| 1IP5 | 2001-11-14 | 1.8 | G105A HUMAN LYSOZYME |
| 1CJ6 | 1999-04-30 | 1.8 | T11A MUTANT HUMAN LYSOZYME |
| 2BQK | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 2LHM | 1992-04-15 | 1.8 | CRYSTAL STRUCTURES OF THE APO-AND HOLOMUTANT HUMAN LYSOZYMES WITH AN INTRODUCED CA2+ BINDING SITE |
| 1GB9 | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 2BQJ | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1CJ7 | 1999-04-30 | 1.8 | T11V MUTANT HUMAN LYSOZYME |
| 1C43 | 1999-08-20 | 1.8 | MUTANT HUMAN LYSOZYME WITH FOREIGN N-TERMINAL RESIDUES |
| 1GF5 | 2001-04-18 | 1.8 | BURIED POLAR MUTANT HUMAN LYSOZYME |
| 1WQM | 1998-07-01 | 1.8 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 2BQE | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1B7N | 1999-01-27 | 1.8 | VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES |
| 2BQO | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1GFE | 2000-12-20 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 2BQH | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1OUB | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V100A MUTANT |
| 1GAZ | 2000-07-27 | 1.8 | Crystal Structure of Mutant Human Lysozyme Substituted at the Surface Positions |
| 1GFV | 2000-12-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 2BQA | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1IP1 | 2001-11-14 | 1.8 | G37A HUMAN LYSOZYME |
| 1GBX | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1GF0 | 2001-04-18 | 1.8 | BURIED POLAR MUTANT HUMAN LYSOZYME |
| 1IP3 | 2001-11-14 | 1.8 | G68A HUMAN LYSOZYME |
| 1LHH | 1994-01-31 | 1.8 | ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS |
| 2MEH | 1998-07-15 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1LZ4 | 1993-10-31 | 1.8 | ENTHALPIC DESTABILIZATION OF A MUTANT HUMAN LYSOZYME LACKING A DISULFIDE BRIDGE BETWEEN CYSTEINE-77 AND CYSTEINE-95 |
| 1B7O | 1999-02-02 | 1.8 | VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES |
| 1CJ9 | 1999-04-30 | 1.8 | T40V MUTANT HUMAN LYSOZYME |
| 1GFT | 2000-12-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1GFU | 2000-12-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1WQP | 1998-07-01 | 1.8 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 2HEA | 1998-01-14 | 1.8 | CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY |
| 1JKD | 1997-05-15 | 1.8 | HUMAN LYSOZYME MUTANT WITH TRP 109 REPLACED BY ALA |
| 1OUI | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V93A MUTANT |
| 1GF6 | 2001-04-18 | 1.8 | BURIED POLAR MUTANT HUMAN LYSOZYME |
| 1GF4 | 2001-04-18 | 1.8 | BURIED POLAR MUTANT HUMAN LYSOZYME |
| 2BQM | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1GBO | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1GB0 | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1C45 | 1999-08-20 | 1.8 | MUTANT HUMAN LYSOZYME WITH FOREIGN N-TERMINAL RESIDUES |
| 2MEI | 1998-07-15 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1CKF | 1999-04-30 | 1.8 | T52A MUTANT HUMAN LYSOZYME |
| 1GF9 | 2000-12-20 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1LHK | 1994-01-31 | 1.8 | ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS |
| 2BQL | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 2MEE | 1998-07-15 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1CKC | 1999-04-30 | 1.8 | T43A MUTANT HUMAN LYSOZYME |
| 1GFA | 2000-12-20 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1LOZ | 1997-04-01 | 1.8 | AMYLOIDOGENIC VARIANT (I56T) VARIANT OF HUMAN LYSOZYME |
| 1YAP | 1996-04-03 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS |
| 2HED | 1998-01-14 | 1.8 | CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY |
| 1GB3 | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1WQN | 1998-07-01 | 1.8 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1GEZ | 2001-04-18 | 1.8 | BURIED POLAR MUTANT HUMAN LYSOZYME |
| 1GDX | 2000-11-08 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS |
| 3LHM | 1992-04-15 | 1.8 | CRYSTAL STRUCTURES OF THE APO-AND HOLOMUTANT HUMAN LYSOZYMES WITH AN INTRODUCED CA2+ BINDING SITE |
| 1CKD | 1999-04-30 | 1.8 | T43V MUTANT HUMAN LYSOZYME |
| 1GB6 | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1GF7 | 2001-04-18 | 1.8 | BURIED POLAR MUTANT HUMAN LYSOZYME |
| 2MEG | 1998-07-15 | 1.8 | CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS. |
| 1EQ4 | 2000-04-19 | 1.8 | CRYSTAL STRUCTURES OF SALT BRIDGE MUTANTS OF HUMAN LYSOZYME |
| 1YAN | 1996-04-03 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS |
| 1YAO | 1996-04-03 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS |
| 207L | 1996-10-14 | 1.8 | MUTANT HUMAN LYSOZYME C77A |
| 1GFJ | 2000-12-20 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1I1Z | 2001-02-28 | 1.8 | MUTANT HUMAN LYSOZYME (Q86D) |
| 1GDW | 2000-11-01 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS |
| 2HEE | 1998-01-14 | 1.8 | CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY |
| 1GAY | 2000-06-29 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1I22 | 2001-02-28 | 1.8 | MUTANT HUMAN LYSOZYME (A83K/Q86D/A92D) |
| 1GFH | 2000-12-20 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1HNL | 1995-02-14 | 1.8 | CRYSTAL STRUCTURE OF A GLUTATHIONYLATED HUMAN LYSOZYME: A FOLDING INTERMEDIATE MIMIC IN THE FORMATION OF A DISULFIDE BOND |
| 1IP4 | 2001-11-14 | 1.8 | G72A HUMAN LYSOZYME |
| 1YAQ | 1996-04-03 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS |
| 1GE0 | 2000-11-08 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS |
| 1GFR | 2000-12-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1B7R | 1999-02-02 | 1.8 | VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES |
| 1WQO | 1998-07-01 | 1.8 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 2BQN | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1GBZ | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 2ZWB | 2009-12-08 | 1.8 | Neutron crystal structure of wild type human lysozyme in D2O |
| 1CJ8 | 1999-04-30 | 1.8 | T40A MUTANT HUMAN LYSOZYME |
| 1OUE | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V125A MUTANT |
| 2BQF | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 2HEF | 1998-01-14 | 1.8 | CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY |
| 1IP6 | 2001-11-14 | 1.8 | G127A HUMAN LYSOZYME |
| 1GBW | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1GB7 | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1B5U | 1999-01-20 | 1.8 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANT |
| 1LZ6 | 1993-10-31 | 1.8 | STRUCTURAL AND FUNCTIONAL ANALYSES OF THE ARG-GLY-ASP SEQUENCE INTRODUCED INTO HUMAN LYSOZYME |
| 1GFK | 2000-12-20 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 2ZIL | 2009-01-06 | 1.8 | Crystal Structure of Human Lysozyme from Urine |
| 1EQ5 | 2000-04-19 | 1.8 | CRYSTAL STRUCTURES OF SALT BRIDGE MUTANTS OF HUMAN LYSOZYME |
| 2BQC | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1INU | 2000-12-20 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1GE4 | 2000-11-08 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS |
| 1GF8 | 2000-12-20 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 2BQG | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1OUF | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V130A MUTANT |
| 2HEC | 1998-01-14 | 1.8 | CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY |
| 1LHJ | 1994-01-31 | 1.8 | ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS |
| 1GB5 | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1OUD | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V121A MUTANT |
| 2BQB | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 2BQI | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1GF3 | 2001-04-18 | 1.8 | BURIED POLAR MUTANT HUMAN LYSOZYME |
| 2MEF | 1998-07-15 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1OUH | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V74A MUTANT |
| 1OUG | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V2A MUTANT |
| 1GBY | 2000-07-27 | 1.8 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS |
| 1LYY | 1997-04-01 | 1.8 | AMYLOIDOGENIC VARIANT (ASP67HIS) OF HUMAN LYSOZYME |
| 1IX0 | 2003-07-22 | 1.8 | I59A-3SS human lysozyme |
| 1OUC | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V110A MUTANT |
| 1WQR | 1998-07-01 | 1.8 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1OUA | 1997-02-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE I56T MUTANT |
| 2BQD | 1998-08-12 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 2MED | 1998-07-15 | 1.8 | CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME |
| 1LHI | 1994-01-31 | 1.8 | ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS |
| 2ZIK | 2009-01-06 | 1.81 | Crystal Structure of Human Lysozyme from Pichia pastoris |
| 3EBA | 2008-12-02 | 1.85 | CAbHul6 FGLW mutant (humanized) in complex with human lysozyme |
| 1QSW | 2001-08-08 | 1.85 | CRYSTAL STRUCTURE ANALYSIS OF A HUMAN LYSOZYME MUTANT W64C C65A |
| 1OP9 | 2003-10-14 | 1.86 | Complex of human lysozyme with camelid VHH HL6 antibody fragment |
| 2ZIJ | 2009-01-06 | 1.9 | Crystal Structure of Human Lysozyme Expressed in E. coli. |
| 1I20 | 2001-02-28 | 1.9 | MUTANT HUMAN LYSOZYME (A92D) |
| 1IP7 | 2001-11-14 | 1.9 | G129A HUMAN LYSOZYME |
| 4I0C | 2013-10-09 | 1.95 | The structure of the camelid antibody cAbHuL5 in complex with human lysozyme |
| 1D6Q | 2000-01-21 | 1.96 | HUMAN LYSOZYME E102 MUTANT LABELLED WITH 2',3'-EPOXYPROPYL GLYCOSIDE OF N-ACETYLLACTOSAMINE |
| 1CKH | 1999-04-30 | 2.0 | T70V MUTANT HUMAN LYSOZYME |
| 1B5X | 1999-01-20 | 2.0 | Contribution of hydrogen bonds to the conformational stability of human lysozyme: calorimetry and x-ray analysis of six ser->ala mutants |
| 1B7Q | 1999-02-02 | 2.0 | VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES |
| 1DI4 | 1999-12-08 | 2.0 | ROLE OF AMINO ACID RESIDUES AT TURNS IN THE CONFORMATIONAL STABILITY AND FOLDING OF HUMAN LYSOZYME |
| 1B7S | 1999-02-02 | 2.0 | VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES |
| 1B7P | 1999-01-25 | 2.0 | VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES |
| 1GE2 | 2000-11-08 | 2.0 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS |
| 1UBZ | 2003-04-22 | 2.0 | Crystal structure of Glu102-mutant human lysozyme doubly labeled with 2',3'-epoxypropyl beta-glycoside of N-acetyllactosamine |
| 3LN2 | 2011-02-16 | 2.04 | Crystal Structure of a Charge Engineered Human Lysozyme Variant |
| 1REM | 1998-07-15 | 2.1 | HUMAN LYSOZYME WITH MAN-B1,4-GLCNAC COVALENTLY ATTACHED TO ASP53 |
| 1GEV | 2001-04-18 | 2.1 | BURIED POLAR MUTANT HUMAN LYSOZYME |
| 1B5W | 1999-01-20 | 2.17 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANTS |
| 1B5V | 1999-01-20 | 2.17 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANTS |
| 1CKG | 1999-05-05 | 2.2 | T52V MUTANT HUMAN LYSOZYME |
| 1B5Z | 1999-02-02 | 2.2 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANTS |
| 1B5Y | 1999-01-20 | 2.2 | CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANTS |
| 2MEA | 1998-07-15 | 2.2 | CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS |
| 1DI5 | 1999-12-08 | 2.2 | ROLE OF AMINO ACID RESIDUES AT TURNS IN THE CONFORMATIONAL STABILITY AND FOLDING OF HUMAN LYSOZYME |
| 208L | 1996-10-14 | 2.2 | MUTANT HUMAN LYSOZYME C77A |
| 2MEC | 1998-07-15 | 2.2 | CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS |
| 2HEB | 1998-01-28 | 2.2 | CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY |
| 1B7M | 1999-01-27 | 2.2 | VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES |
| 1C46 | 1999-08-20 | 2.2 | MUTANT HUMAN LYSOZYME WITH FOREIGN N-TERMINAL RESIDUES |
| 1D6P | 2000-01-21 | 2.23 | HUMAN LYSOZYME L63 MUTANT LABELLED WITH 2',3'-EPOXYPROPYL N,N'-DIACETYLCHITOBIOSE |
| 1RE2 | 1999-05-05 | 2.3 | HUMAN LYSOZYME LABELLED WITH TWO 2',3'-EPOXYPROPYL BETA-GLYCOSIDE OF N-ACETYLLACTOSAMINE |
| 1C7P | 2000-04-05 | 2.4 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME WITH FOUR EXTRA RESIDUES (EAEA) AT THE N-TERMINAL |
| 1IOC | 2002-10-09 | 2.4 | CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME, EAEA-I56T |
| 5LVK | 2016-09-28 | 2.49 | Human Lysozyme soaked with [H2Ind][trans-RuCl4(DMSO)(HInd)] |
| 1W08 | 2004-06-10 | 2.5 | STRUCTURE OF T70N HUMAN LYSOZYME |