Contribution of surface salt bridges to protein stability.


Abstract

The role of surface salt bridges in protein stabilization has been a source of controversy. Here we present the NMR structure of a hyperthermophilic rubredoxin variant (PFRD-XC4) and the thermodynamic analysis of two surface salt bridges by double mutant cycles. This analysis shows that the surface side chain to side chain salt bridge between Lys 6 and Glu 49 does not stabilize PFRD-XC4. The main chain to side chain salt bridge between the N-terminus and Glu 14 was, however, found to stabilize PFRD-XC4 by 1. 5 kcal mol(-)(1). The entropic cost of making a surface salt bridge involving the protein's backbone is reduced, since the backbone has already been immobilized upon protein folding. Study holds ProTherm entries: 7918, 7919, 7920, 7921, 7922, 7923, 7924, 7925, 7926, 7927, 7928, 7929, 7930, 7931, 7932, 7933, 7934, 7935, 7936, 7937, 7938, 7939, 7940, 7941, 7942 Extra Details: surface salt bridges; protein stabilization; hyperthermophilic,rubredoxin; protein folding

Submission Details

ID: gcNU3jda

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:35 p.m.

Version: 1

Publication Details
Strop P;Mayo SL,Biochemistry (2000) Contribution of surface salt bridges to protein stability. PMID:10684603
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1BQ8 1998-08-22T00:00:00+0000 1.1 Rubredoxin (Methionine Mutant) from Pyrococcus Furiosus
1BQ9 1998-08-22T00:00:00+0000 1.2 Rubredoxin (Formyl Methionine Mutant) from Pyrococcus Furiosus
1BRF 1998-08-24T00:00:00+0000 0.95 Rubredoxin (Wild Type) from Pyrococcus Furiosus
1CAA 1992-05-18T00:00:00+0000 1.8 X-RAY CRYSTAL STRUCTURES OF THE OXIDIZED AND REDUCED FORMS OF THE RUBREDOXIN FROM THE MARINE HYPERTHERMOPHILIC ARCHAEBACTERIUM PYROCOCCUS FURIOSUS
1CAD 1992-05-18T00:00:00+0000 1.8 X-RAY CRYSTAL STRUCTURES OF THE OXIDIZED AND REDUCED FORMS OF THE RUBREDOXIN FROM THE MARINE HYPERTHERMOPHILIC ARCHAEBACTERIUM PYROCOCCUS FURIOSUS
1IU5 2002-02-27T00:00:00+0000 1.5 X-ray Crystal Structure of the rubredoxin mutant from Pyrococcus Furiosus
1IU6 2002-02-27T00:00:00+0000 1.6 Neutron Crystal Structure of the rubredoxin mutant from Pyrococcus Furiosus
1QCV 1999-05-10T00:00:00+0000 0 RUBREDOXIN VARIANT (PFRD-XC4) FOLDS WITHOUT IRON
1RWD 2003-12-16T00:00:00+0000 0 Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings
1VCX 2004-03-17T00:00:00+0000 1.5 Neutron Crystal Structure of the Wild Type Rubredoxin from Pyrococcus Furiosus at 1.5A Resolution

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Rubredoxin P24297 RUBR_PYRFU