Differential scanning calorimetric studies on bovine serum albumin: I. Effects of pH and ionic strength.


Abstract

Using defatted and SH-blocked bovine serum albumin (BSA), the measurement of differential scanning calorimetry (d.s.c.) was performed in the range pH 3-11 and ionic strength 0.001-1 M. The shape of the d.s.c. curve was classified into four regimes: (i) the curve with no peak, (ii) that with a peak, (iii) that with a peak having a shoulder, and (iv) that with two peaks. The presence of two peaks was interpreted by the concept of 'heat-induced transition'. The BSA molecule is composed of two domains, thermodynamically independent owing to the formation of a crevice in BSA in a particular range of pH and ionic strength; this gives two peaks in the d.s.c. curve. The enthalpy (delta H) from the d.s.c. curve was plotted against pH and against the NaCl concentration. The value of delta H increased with the increase in the ionic strength in the pH range 5.6-9.0. The temperature of thermal denaturation (the temperature of the peak maximum, Td) was raised with the increase in the ionic strength in the pH range 4.5-9.0, but was lowered in the pH range 3.5-4.0. BSA was stabilized in the neutral-alkaline pH range by the presence of NaCl, but was destabilized in the acidic pH range. Study holds ProTherm entries: 11292, 11293, 11294, 11295, 11296, 11297, 11298, 11299, 11300, 11301, 11302 Extra Details: buffer concentration adjusted by NaOH thermal denaturation; bovine serum albumin;,differential scanning calorimetry

Submission Details

ID: gLBtV4Y3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:42 p.m.

Version: 1

Publication Details
Yamasaki M;Yano H;Aoki K,Int. J. Biol. Macromol. (1990) Differential scanning calorimetric studies on bovine serum albumin: I. Effects of pH and ionic strength. PMID:2096908
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Serum albumin P02769 ALBU_BOVIN
92.4 Serum albumin P14639 ALBU_SHEEP