Comparative study of the stability of the folding intermediates of the calcium-binding lysozymes.


Abstract

Unfolding profiles of two calcium-binding lysozymes, equine milk lysozyme and pigeon egg-white lysozyme, were obtained by circular dichroism and proton NMR measurements. Equine lysozyme unfolds through a stable molten globule intermediate. The molten globule of equine lysozyme was characterized as more ordered than that of bovine alpha-lactalbumin. On the other hand, pigeon lysozyme unfolds by a two-state mechanism and the intermediate could not be observed in guanidine or thermal unfolding, the same as with conventional non-calcium-binding lysozymes. Thus, from the point of view of the unfolding profile, equine lysozyme belongs to the group of alpha-lactalbumin, but pigeon lysozyme belongs to the conventional lysozyme group. Study holds ProTherm entries: 10366, 10367, 10368 Extra Details: Transition I calcium-binding lysozyme; alpha-lactalbumin; folding intermediate;,pigeon lysozyme; equine lysozyme

Submission Details

ID: fnniKHft3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:40 p.m.

Version: 1

Publication Details
Nitta K;Tsuge H;Iwamoto H,Int. J. Pept. Protein Res. (1993) Comparative study of the stability of the folding intermediates of the calcium-binding lysozymes. PMID:8458685
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
5XUW 2018-06-27 1.76 Crystal structure of lysozyme from Equus asinus
2EQL 1994-07-31 2.5 CRYSTALLOGRAPHIC STUDIES OF A CALCIUM BINDING LYSOZYME FROM EQUINE MILK AT 2.5 ANGSTROMS RESOLUTION

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Lysozyme C P00708 LYSC_COLLI
97.7 Lysozyme C, milk isozyme P11375 LYSC_EQUAS
100.0 Lysozyme C, milk isozyme P11376 LYSC1_HORSE