Thermodynamic stability of carbonic anhydrase: measurements of binding affinity and stoichiometry using ThermoFluor.


Abstract

ThermoFluor (a miniaturized high-throughput protein stability assay) was used to analyze the linkage between protein thermal stability and ligand binding. Equilibrium binding ligands increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. Binding constants (K(b)) were measured by examining the systematic effect of ligand concentration on protein stability. The precise ligand effects depend on the thermodynamics of protein stability: in particular, the unfolding enthalpy. An extension of current theoretical treatments was developed for tight binding inhibitors, where ligand effect on T(m) can also reveal binding stoichiometry. A thermodynamic analysis of carbonic anhydrase by differential scanning calorimetry (DSC) enabled a dissection of the Gibbs free energy of stability into enthalpic and entropic components. Under certain conditions, thermal stability increased by over 30 degrees C; the heat capacity of protein unfolding was estimated from the dependence of calorimetric enthalpy on T(m). The binding affinity of six sulfonamide inhibitors to two isozymes (human type 1 and bovine type 2) was analyzed by both ThermoFluor and isothermal titration calorimetry (ITC), resulting in a good correlation in the rank ordering of ligand affinity. This combined investigation by ThermoFluor, ITC, and DSC provides a detailed picture of the linkage between ligand binding and protein stability. The systematic effect of ligands on stability is shown to be a general tool to measure affinity. Study holds ProTherm entries: 19164, 19165, 19166, 19167, 19168 Extra Details: 0.5 mM EDTA, 50 microM ANS and 100 microM ACTAZ were added in the experiment. ligand binding; binding constants; enthalpic; entropic

Submission Details

ID: fC2H84q5

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:51 p.m.

Version: 1

Publication Details
Matulis D;Kranz JK;Salemme FR;Todd MJ,Biochemistry (2005) Thermodynamic stability of carbonic anhydrase: measurements of binding affinity and stoichiometry using ThermoFluor. PMID:15794662
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1G6V 2000-11-08T00:00:00+0000 3.5 Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase
1V9E 2004-01-26T00:00:00+0000 1.95 Crystal Structure Analysis of Bovine Carbonic Anhydrase II
1V9I 2004-01-26T00:00:00+0000 2.95 Crystal Structure Analysis of the site specific mutant (Q253C) of bovine carbonic anhydrase II
4CNR 2014-01-24T00:00:00+0000 2.29 Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture
4CNV 2014-01-25T00:00:00+0000 1.62 Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture
4CNW 2014-01-25T00:00:00+0000 2.03 Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture
4CNX 2014-01-25T00:00:00+0000 1.23 Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture
5A25 2015-05-12T00:00:00+0000 1.9 Rational engineering of a mesophilic carbonic anhydrase to an extreme halotolerant biocatalyst
5EZT 2015-11-26T00:00:00+0000 1.54 Peracetylated Bovine Carbonic Anhydrase II
6SKS 2019-08-16T00:00:00+0000 1.75 Crystal structure of bovine carbonic anhydrase II in complex with a benzenesulfonamide-based ligand (SH1)

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Carbonic anhydrase 2 P86277 BIOR_THESB
92.3 Carbonic anhydrase 2 P00922 CAH2_SHEEP
100.0 Carbonic anhydrase 2 P00921 CAH2_BOVIN
100.0 A Carbonic anhydrase 2 P00918 CAH2_HUMAN