Equilibria and kinetics of folding of gelsolin domain 2 and mutants involved in familial amyloidosis-Finnish type.


Abstract

Mutations D187N and D187Y in domain 2 of the actin-regulating protein gelsolin cause familial amyloidosis-Finnish type (FAF). We have constructed and expressed a recombinant version of gelsolin domain 2 that is sufficiently stable for kinetic and equilibrium measurements. The wild-type domain and the two amyloidogenic mutants fold via simple two-state kinetics without the accumulation of an intermediate. Unfolding kinetics exhibits significant curvature with increasing urea concentration, indicating that the transition state for unfolding becomes more native-like under increasingly denaturing conditions in accordance with the Hammond postulate. Mutations D187N and D187Y destabilize gelsolin domain 2 by 1.22 and 2.16 kcal. mol(-1) (1 kcal = 4.18 kJ) respectively. The mutations do not prevent disulfide bond formation despite their direct contiguity with a cysteine residue involved in disulfide linkage. The destabilization conferred on gelsolin domain 2 by the FAF mutations is sufficient to predict that an appreciable fraction is unfolded and, therefore, extremely susceptible to proteolysis at body temperature. Study holds ProTherm entries: 6490, 6491, 6492, 6493 Extra Details: protein; stability; denaturation; Hammond; two-state

Submission Details

ID: ezWdA95J

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:32 p.m.

Version: 1

Publication Details
Isaacson RL;Weeds AG;Fersht AR,Proc. Natl. Acad. Sci. U.S.A. (1999) Equilibria and kinetics of folding of gelsolin domain 2 and mutants involved in familial amyloidosis-Finnish type. PMID:10500162
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3B5U 2008-04-15 Actin filament model from extended form of acromsomal bundle in the Limulus sperm
5H3N 2017-11-01 Solution structure of human Gelsolin protein domain 1 at pH 7.3
1SOL 1996-03-08 A PIP2 AND F-ACTIN-BINDING SITE OF GELSOLIN, RESIDUE 150-169 (NMR, AVERAGED STRUCTURE)
2W49 2010-05-05 ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE
2W4U 2010-08-25 Isometrically contracting insect asynchronous flight muscle quick frozen after a length step
5H3M 2017-11-01 Solution structure of human Gelsolin protein domain 1 at pH 5.0
5FAF 2016-10-05 1.05 N184K pathological variant of gelsolin domain 2 (orthorhombic form)
6I4E 2019-06-26 1.22 Crystal Structure of Plasmodium falciparum actin I in the Mg-ADP state
6I4D 2019-06-26 1.24 Crystal Structure of Plasmodium falciparum actin I in the Mg-K-ATP/ADP state
4B1Y 2013-07-31 1.29 Structure of the Phactr1 RPEL-3 bound to G-actin
4CBU 2014-04-30 1.3 Crystal structure of Plasmodium falciparum actin I
2FXU 2006-03-07 1.35 X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution.
6I4H 2019-06-26 1.4 Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Ca-ATP state
4K41 2014-10-01 1.4 Crystal structure of actin in complex with marine macrolide kabiramide C
5MVV 2017-07-12 1.4 Crystal structure of Plasmodium falciparum actin I- gelsolin segment 1 -CdATP complex
2V52 2008-11-25 1.45 Structure of MAL-RPEL2 complexed to G-actin
1WUA 2006-02-14 1.45 The structure of Aplyronine A-actin complex
2Q0U 2007-07-17 1.45 Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin
1QZ5 2003-11-11 1.45 Structure of rabbit actin in complex with kabiramide C
2PBD 2007-11-13 1.5 Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*
6I4F 2019-06-26 1.5 Crystal Structure of Plasmodium falciparum actin I (A272W mutant) in the Mg-K-ATP/ADP state
6I4J 2019-06-26 1.5 Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Mg-ADP state
3MN5 2010-06-02 1.5 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
5ZZA 2018-10-10 1.53 OdinProfilin/Rabbit Actin Complex
1J6Z 2001-08-15 1.54 UNCOMPLEXED ACTIN
2FH1 2006-06-13 1.55 C-terminal half of gelsolin soaked in low calcium at pH 4.5
2ASM 2005-10-11 1.6 Structure of Rabbit Actin In Complex With Reidispongiolide A
3CIP 2008-08-19 1.6 Complex of Dictyostelium Discoideum Actin with Gelsolin
1QZ6 2003-11-11 1.6 Structure of rabbit actin in complex with jaspisamide A
1S22 2004-02-17 1.6 Absolute Stereochemistry of Ulapualide A
2ASP 2005-10-11 1.64 Structure of Rabbit Actin In Complex With Reidispongiolide C
1KCQ 2002-01-04 1.65 Human Gelsolin Domain 2 with a Cd2+ bound
6MBK 2018-12-19 1.69 SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, First P212121 Crystal Form
5FAE 2016-10-05 1.7 N184K pathological variant of gelsolin domain 2 (trigonal form)
3CI5 2008-08-19 1.7 Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin
2ASO 2005-10-11 1.7 Structure of Rabbit Actin In Complex With Sphinxolide B
5O2Z 2017-11-08 1.7 Domain swap dimer of the G167R variant of gelsolin second domain
2Q0R 2007-07-17 1.7 Structure of Pectenotoxin-2 Bound to Actin
6OX0 2019-08-21 1.75 SETD3 in Complex with an Actin Peptide with Sinefungin Replacing SAH as Cofactor
1D4X 2001-05-02 1.75 Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin Segment 1 at 1.75 A resolution.
3SJH 2012-01-25 1.75 Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP-Latrunculin A
4B1V 2012-11-07 1.75 Structure of the Phactr1 RPEL-N domain bound to G-actin
4H03 2013-02-20 1.75 Crystal structure of NAD+-Ia-actin complex
6MBJ 2018-12-19 1.78 SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, P21 Crystal Form
4PKG 2014-07-30 1.8 Complex of ATP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
2A40 2005-11-01 1.8 Ternary complex of the WH2 domain of WAVE with Actin-DNAse I
2HF3 2006-08-29 1.8 Crystal structure of monomeric Actin in the ADP bound state
4B1X 2013-07-31 1.8 Structure of the Phactr1 RPEL-2 bound to G-actin
1NLV 2003-01-21 1.8 Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human Gelsolin Segment 1
2PAV 2007-10-23 1.8 Ternary complex of Profilin-Actin with the Last Poly-Pro of Human VASP
3W3D 2013-01-30 1.8 Crystal structure of smooth muscle G actin DNase I complex
1NM1 2003-01-21 1.8 Crystal Structure of D. Dicsoideum Actin Complexed With Gelsolin Segment 1 and Mg ATP at 1.8 A Resolution
3EKS 2008-10-07 1.8 Crystal Structure of Monomeric Actin bound to Cytochalasin D
2HF4 2006-08-29 1.8 Crystal structure of Monomeric Actin in its ATP-bound state
5YEE 2018-09-19 1.81 Crystal structure of LokiProfilin1/Rabbit Actin Complex
6I4K 2019-06-26 1.83 Crystal Structure of Plasmodium falciparum actin I (G115A mutant) in the Ca-ATP state
6I4L 2019-06-26 1.83 Crystal Structure of Plasmodium falciparum actin I (G115A mutant) in the Mg-K-ATP/ADP state
1NWK 2003-10-14 1.85 CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE
2A42 2005-11-01 1.85 Actin-DNAse I Complex
6I4M 2019-06-26 1.87 Crystal Structure of Plasmodium berghei actin II in the Mg-ADP state
2HMP 2006-09-19 1.9 Uncomplexed actin cleaved with protease ECP32
1YAG 1999-10-09 1.9 STRUCTURE OF THE YEAST ACTIN-HUMAN GELSOLIN SEGMENT 1 COMPLEX
6H1F 2019-01-23 1.9 Structure of the nanobody-stabilized gelsolin D187N variant (second domain)
1NMD 2003-02-04 1.9 Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP
6I4I 2019-06-26 1.9 Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Mg-K-ADP-AlFn state
2GWJ 2006-08-29 1.9 SpvB ADP-ribosylated actin: hexagonal crystal form
4H0Y 2013-02-20 1.94 Crystal structure of NAD+-Ia(E380S)-actin complex
6ICT 2019-02-27 1.95 Structure of SETD3 bound to SAH and methylated actin
4B1W 2013-07-31 1.95 Structure of the Phactr1 RPEL-2 domain bound to actin
6OX1 2019-08-21 1.95 SETD3 in Complex with an Actin Peptide with Target Histidine Partially Methylated
5NOG 2017-07-19 11.0 Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - 'Blocked' state
5NOJ 2017-08-02 11.0 Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - 'OPEN' state
6CXI 2018-10-10 11.0 Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 1
6CXJ 2018-10-10 11.0 Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 2
3BYH 2008-02-19 12.0 Model of actin-fimbrin ABD2 complex
3J8J 2015-01-14 12.0 Tilted state of actin, T1
3J8K 2015-01-14 12.0 Tilted state of actin, T2
3LUE 2010-04-28 15.0 Model of alpha-actinin CH1 bound to F-actin
1C0G 2000-03-01 2.0 CRYSTAL STRUCTURE OF 1:1 COMPLEX BETWEEN GELSOLIN SEGMENT 1 AND A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 228: Q228K/T229A/A230Y/E360H)
2FF3 2006-03-21 2.0 Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin
3M6G 2010-09-08 2.0 Crystal structure of actin in complex with lobophorolide
5YPU 2018-09-26 2.0 Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu MET72NLE WH2-motif peptide
3U8X 2012-01-25 2.0 Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP
6I4G 2019-06-26 2.0 Crystal Structure of Plasmodium falciparum actin I (H74Q) in the Mg-K-ATP state
2GWK 2006-08-29 2.0 SpvB ADP-ribosylated actin: orthorhombic crystal form
3MN7 2010-05-26 2.0 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
1ESV 2000-07-19 2.0 COMPLEX BETWEEN LATRUNCULIN A:RABBIT MUSCLE ALPHA ACTIN:HUMAN GELSOLIN DOMAIN 1
4PL8 2014-10-22 2.0 Structure of rabbit skeletal muscle actin in complex with a hybrid peptide comprising thymosin beta4 and the lysine-rich region of Cordon-Bleu
1P8X 2003-10-14 2.0 The Calcium-Activated C-terminal half of gelsolin
3MN6 2010-06-02 2.0 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
4B1U 2013-07-31 2.0 Structure of the Phactr1 RPEL domain and RPEL motif directed assemblies with G-actin reveal the molecular basis for actin binding cooperativity.
3MN9 2010-05-26 2.0 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
1T44 2004-09-07 2.0 Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation
1YXQ 2005-05-17 2.01 Crystal structure of actin in complex with swinholide A
4H0V 2013-02-20 2.03 Crystal structure of NAD+-Ia(E378S)-actin complex
2FF6 2006-03-21 2.05 Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin
2A3Z 2005-11-01 2.08 Ternary complex of the WH2 domain of WASP with Actin-DNAse I
6OX2 2019-08-21 2.09 SETD3in Complex with an Actin Peptide with the Target Histidine Fully Methylated
3U9Z 2012-01-25 2.09 Crystal structure between actin and a protein construct containing the first beta-thymosin domain of drosophila ciboulot (residues 2-58) with the three mutations N26D/Q27K/D28S
1KXP 2002-06-19 2.1 CRYSTAL STRUCTURE OF HUMAN VITAMIN D-BINDING PROTEIN IN COMPLEX WITH SKELETAL ACTIN
6OX5 2019-08-21 2.1 A SETD3 Mutant (N255A) in Complex with an Actin Peptide with His73 Replaced with Lysine
1YVN 2000-03-23 2.1 THE YEAST ACTIN VAL 159 ASN MUTANT COMPLEX WITH HUMAN GELSOLIN SEGMENT 1.
4PKH 2014-07-30 2.15 Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
6ICV 2019-02-27 2.15 Structure of SETD3 bound to SAH and unmodified actin
3UB5 2012-04-25 2.2 Profilin:actin with a wide open nucleotide cleft
4H0T 2013-02-20 2.2 Crystal structure of Ia-ADPR-actin complex
6MGO 2018-11-21 2.2 Structure of rabbit actin in complex with Mycalolide B
1MDU 2003-01-07 2.2 Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1 (GS-1)
4CBX 2014-04-30 2.2 Crystal structure of Plasmodium berghei actin II
6MBL 2018-12-19 2.2 SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, Second P212121 Crystal Form
3D2U 2008-07-08 2.21 Structure of UL18, a Peptide-Binding Viral MHC Mimic, Bound to a Host Inhibitory Receptor
6OX4 2019-08-21 2.29 A SETD3 Mutant (N255A) in Complex with an Actin Peptide
5ZZB 2018-10-10 2.3 LokiProfilin2/Rabbit Actin Complex
1EQY 2000-05-03 2.3 COMPLEX BETWEEN RABBIT MUSCLE ALPHA-ACTIN: HUMAN GELSOLIN DOMAIN 1
1RDW 2003-12-16 2.3 Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer
3CHW 2008-08-19 2.3 Complex of Dictyostelium discoideum Actin with Profilin and the Last Poly-Pro of Human VASP
4PKI 2014-07-30 2.3 Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin
4H0X 2013-02-20 2.33 Crystal structure of NAD+-Ia(E380A)-actin complex
2V51 2008-11-25 2.35 Structure of MAL-RPEL1 complexed to actin
2VYP 2009-02-24 2.35 Rabbit-muscle G-actin in complex with myxobacterial rhizopodin
3A5N 2010-10-27 2.36 Crystal Structure of a Dictyostelium P109A Ca2+-Actin in Complex with Human Gelsolin Segment 1
5UBO 2017-12-20 2.39 Mical-oxidized Actin complex with Gelsolin Segment 1
3A5O 2010-10-27 2.4 Crystal Structure of a Dictyostelium P109I Ca2+-Actin in Complex with Human Gelsolin Segment 1
1MA9 2003-02-04 2.4 Crystal structure of the complex of human vitamin D binding protein and rabbit muscle actin
1C0F 2000-03-01 2.4 CRYSTAL STRUCTURE OF DICTYOSTELIUM CAATP-ACTIN IN COMPLEX WITH GELSOLIN SEGMENT 1
3A5M 2010-10-27 2.4 Crystal Structure of a Dictyostelium P109I Mg2+-Actin in Complex with Human Gelsolin Segment 1
3A5L 2010-10-27 2.4 Crystal Structure of a Dictyostelium P109A Mg2+-Actin in Complex with Human Gelsolin Segment 1
3U4L 2012-04-25 2.4 Cryocooled bovine profilin:actin crystal structure to 2.4 A
1DEJ 2000-03-01 2.4 CRYSTAL STRUCTURE OF A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 646: Q228K/T229A/A230Y/A231K/S232E/E360H) IN COMPLEX WITH HUMAN GELSOLIN SEGMENT 1
6QRI 2019-07-03 2.4 Structure of rabbit G-actin in complex with chivosazole A
4Z94 2015-10-21 2.4 Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2
4EFH 2012-04-11 2.48 Acanthamoeba Actin complex with Spir domain D
2A5X 2005-08-23 2.49 Crystal Structure of a Cross-linked Actin Dimer
3EKU 2008-10-07 2.5 Crystal Structure of Monomeric Actin bound to Cytochalasin D
1D0N 1999-09-15 2.5 THE CRYSTAL STRUCTURE OF CALCIUM-FREE EQUINE PLASMA GELSOLIN.
2FH2 2006-06-13 2.5 C-terminal half of gelsolin soaked in EGTA at pH 4.5
2Q36 2007-06-05 2.5 Actin Dimer Cross-linked between Residues 191 and 374 and complexed with Kabiramide C
4CBW 2014-04-30 2.5 Crystal structure of Plasmodium berghei actin I with D-loop from muscle actin
1LOT 2002-07-31 2.5 CRYSTAL STRUCTURE OF THE COMPLEX OF ACTIN WITH VITAMIN D-BINDING PROTEIN
1SQK 2004-06-15 2.5 CRYSTAL STRUCTURE OF CIBOULOT IN COMPLEX WITH SKELETAL ACTIN
3EL2 2008-10-07 2.5 Crystal Structure of Monomeric Actin Bound to Ca-ATP
3U9D 2012-01-25 2.5 Crystal Structure of a chimera containing the N-terminal domain (residues 8-24) of drosophila Ciboulot and the C-terminal domain (residues 13-44) of bovine Thymosin-beta4, bound to G-actin-ATP
2Q97 2007-10-16 2.5 Complex of mammalian actin with toxofilin from toxoplasma gondii
2D1K 2006-09-12 2.5 Ternary complex of the WH2 domain of mim with actin-dnase I
3DAW 2008-07-29 2.55 Structure of the actin-depolymerizing factor homology domain in complex with actin
2BTF 1994-06-22 2.55 THE STRUCTURE OF CRYSTALLINE PROFILIN-BETA-ACTIN
2A41 2005-11-01 2.6 Ternary complex of the WH2 Domain of WIP with Actin-DNAse I
5DD2 2016-10-05 2.6 Human gelsolin from residues Glu28 to Arg161 with Calcium
6GVC 2019-03-27 2.6 Structure of ArhGAP12 bound to G-Actin
1P8Z 2003-10-14 2.6 Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156
4S10 2015-06-03 2.61 Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis
2OAN 2007-05-01 2.61 Structure of oxidized beta-actin
5ZZ0 2019-06-05 2.63 HUMAN GELSOLIN FROM RESIDUES GLU28 TO ARG161 WITH CALCIUM
1HLU 1997-10-15 2.65 STRUCTURE OF BOVINE BETA-ACTIN-PROFILIN COMPLEX WITH ACTIN BOUND ATP PHOSPHATES SOLVENT ACCESSIBLE
2Q31 2007-06-05 2.7 Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48.
2Q1N 2007-06-05 2.7 Actin Dimer Cross-linked Between Residues 41 and 374
3HBT 2010-05-05 2.7 The structure of native G-actin
4GY2 2013-02-20 2.71 Crystal structure of apo-Ia-actin complex
4M63 2013-10-23 2.75 Crystal Structure of a Filament-Like Actin Trimer Bound to the Bacterial Effector VopL
3UE5 2012-02-15 2.76 ECP-cleaved Actin in complex with Spir domain D
6FM2 2018-05-16 2.8 CARP domain of mouse cyclase-associated protein 1 (CAP1) bound to ADP-actin
1ATN 1992-07-15 2.8 Atomic structure of the actin:DNASE I complex
2FGH 2006-04-18 2.8 ATP bound gelsolin
3MMV 2010-06-02 2.8 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
3BUZ 2008-05-13 2.81 Crystal structure of ia-bTAD-actin complex
1IJJ 2002-04-15 2.85 THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION
2FH3 2006-06-13 2.87 C-terminal half of gelsolin soaked in low calcium at pH 8
6JCO 2019-06-12 2.88 Crystal structure of calcium free human gelsolin amyloid mutant D187N
3M1F 2010-09-15 2.89 Crosslinked complex of actin with first W domain of Vibrio parahaemolyticus VopL
4K43 2014-10-01 2.9 Crystal structure of actin in complex with synthetic AplC tail analogue GC04 [N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide]
4K42 2014-10-01 2.9 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate]
4JHD 2013-06-19 2.91 Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu
6JEH 2019-06-12 2.95 Crystal structure of calcium free human gelsolin amyloid mutant D187Y
6JEG 2019-06-12 2.97 Crystal structure of calcium free human gelsolin amyloid mutant G167R
4RWT 2015-10-14 2.98 Structure of actin-Lmod complex
1H1V 2003-01-24 2.99 gelsolin G4-G6/actin complex
2Y83 2011-03-30 22.9 Actin filament pointed end
5MVA 2017-11-29 27.7 Structure of the thin filament at high calcium concentration
5MVY 2018-02-14 28.4 Thin Filament at low calcium concentration
2FH4 2006-06-13 3.0 C-terminal half of gelsolin soaked in EGTA at pH 8
3FFK 2009-10-06 3.0 Crystal structure of human Gelsolin domains G1-G3 bound to Actin
1RGI 2004-07-27 3.0 Crystal structure of gelsolin domains G1-G3 bound to actin
1RFQ 2003-12-16 3.0 Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer
3TU5 2012-09-26 3.0 Actin complex with Gelsolin Segment 1 fused to Cobl segment
5WFN 2017-08-30 3.0 Revised model of leiomodin 2-mediated actin regulation (alternate refinement of PDB 4RWT)
3FFN 2009-10-06 3.0 Crystal structure of calcium-free human gelsolin
1NPH 2003-05-13 3.0 Gelsolin Domains 4-6 in Active, Actin Free Conformation Identifies Sites of Regulatory Calcium Ions
1Y64 2005-01-18 3.05 Bni1p Formin Homology 2 Domain complexed with ATP-actin
6DJN 2019-02-27 3.1 Cryo-EM structure of ADP-Pi-actin filaments
2YJE 2011-07-06 3.1 Oligomeric assembly of actin bound to MRTF-A
6DJM 2019-02-27 3.1 Cryo-EM structure of AMPPNP-actin filaments
6C1D 2018-01-31 3.2 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
2VCP 2008-11-04 3.2 Crystal structure of N-Wasp VC domain in complex with skeletal actin
3CJB 2008-03-25 3.21 Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1
4B1Z 2012-11-07 3.3 Structure of the Phactr1 RPEL domain bound to G-actin
2ZWH 2009-01-20 3.3 Model for the F-actin structure
6FHL 2018-06-13 3.3 Cryo-EM structure of F-actin in complex with ADP-Pi
5OOF 2018-06-13 3.4 Cryo-EM structure of F-actin in complex with ADP-BeFx
4EAH 2012-12-12 3.4 Crystal structure of the formin homology 2 domain of FMNL3 bound to actin
3TPQ 2011-10-12 3.45 Crystal structure of wild-type MAL RPEL domain in complex with five G-actins
4WYB 2015-08-19 3.49 Structure of the Bud6 flank domain in complex with actin
6F1T 2018-01-17 3.5 Cryo-EM structure of two dynein tail domains bound to dynactin and BICDR1
1LCU 2002-05-01 3.5 Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution
2YJF 2011-07-06 3.5 Oligomeric assembly of actin bound to MRTF-A
6D8C 2018-09-19 3.54 Cryo-EM structure of FLNaABD E254K bound to phalloidin-stabilized F-actin
5JLF 2016-06-15 3.6 Structure of the F-actin-tropomyosin complex (reprocessed)
6DJO 2019-02-27 3.6 Cryo-EM structure of ADP-actin filaments
5OOC 2018-06-13 3.6 Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP
5OOE 2018-06-13 3.6 Cryo-EM structure of F-actin in complex with AppNHp (AMPPNP)
5OOD 2018-06-13 3.7 Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP-Pi
3J8A 2014-12-10 3.7 Structure of the F-actin-tropomyosin complex
6C1G 2018-01-31 3.8 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
5YU8 2018-05-23 3.8 Cofilin decorated actin filament
6AV9 2018-01-17 3.9 CryoEM structure of Mical Oxidized Actin (Class 1)
3CJC 2008-03-25 3.9 Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with DNase I and Gelsolin-segment 1
5JLH 2016-06-15 3.9 Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution
6C1H 2018-01-31 3.9 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
6AVB 2018-01-17 3.9 CryoEM structure of Mical Oxidized Actin (Class 1)
5ADX 2015-12-30 4.0 CryoEM structure of dynactin complex at 4.0 angstrom resolution
5ONV 2018-06-13 4.1 Cryo-EM structure of F-actin in complex with ADP
6BNV 2018-01-10 4.6 CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine
6BNP 2018-01-10 4.6 CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state
3J8I 2015-01-14 4.7 Near-Atomic Resolution for One State of F-Actin
5H53 2017-01-18 5.2 The structure of rabbit skeletal muscle actomyosin rigor complex at 5.2 angstrom.
6BNQ 2018-01-10 5.5 CryoEM structure of Myosin VI-Actin complex in the ADP state
6BNW 2018-01-10 5.5 CryoEM structure of Myosin VI-Actin complex in the ADP state, backbone-averaged with side chains truncated to alanine
6BNO 2018-01-10 5.5 Structure of bare actin filament
3G37 2010-11-03 6.0 Cryo-EM structure of actin filament in the presence of phosphate
6BIH 2018-09-19 6.0 The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State
3MFP 2010-09-29 6.6 Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map
6F38 2018-01-17 6.7 Cryo-EM structure of two dynein tail domains bound to dynactin and HOOK3
6ANU 2017-11-22 7.0 Cryo-EM structure of F-actin complexed with the beta-III-spectrin actin-binding domain
3M3N 2010-07-28 7.0 Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains
6BNU 2018-01-10 7.5 Structure of bare actin filament, backbone-averaged with sidechains truncated to alanine
3JBJ 2015-11-04 7.6 Cryo-EM reconstruction of F-actin
4A7F 2012-08-01 7.7 Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 3)
3J82 2015-05-20 7.7 Electron cryo-microscopy of DNGR-1 in complex with F-actin
4A7H 2012-08-01 7.8 Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2)
4V0U 2015-03-25 7.88 The crystal structure of ternary PP1G-PPP1R15B and G-actin complex
1O1F 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O19 2002-11-27 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1A 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1D 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1M8Q 2002-09-10 70.0 Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle
1O1B 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O18 2002-11-27 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1E 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1G 2002-12-11 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1O1C 2002-12-04 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
1MVW 2002-11-20 70.0 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
3J4K 2013-09-25 8.0 Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state
5NOL 2017-07-19 8.0 Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - 'Closed' state
4A7L 2012-08-01 8.1 Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 1)
3JBK 2015-11-04 8.2 Cryo-EM reconstruction of the metavinculin-actin interface
5AFU 2015-03-11 8.2 Cryo-EM structure of dynein tail-dynactin-BICD2N complex
6F3A 2018-01-17 8.2 Cryo-EM structure of a single dynein tail domain bound to dynactin and BICD2N
3JBI 2015-11-04 8.5 MDFF model of the vinculin tail domain bound to F-actin
4A7N 2012-08-01 8.9 Structure of bare F-actin filaments obtained from the same sample as the Actin-Tropomyosin-Myosin Complex
6G2T 2018-10-17 9.0 human cardiac myosin binding protein C C1 Ig-domain bound to native cardiac thin filament
3J0S 2011-12-21 9.0 Remodeling of actin filaments by ADF cofilin proteins
5KG8 2016-09-07 9.1 Rigor myosin X co-complexed with an actin filament
3B63 2008-11-18 9.5 Actin filament model in the extended form of acromsomal bundle in the Limulus sperm

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
97.6 G Major actin Q68FP1 GELS_RAT
97.6 G Major actin P13020 GELS_MOUSE
98.4 G Major actin Q28372 GELS_HORSE
99.2 G Major actin P20305 GELS_PIG
99.2 G Major actin Q3SX14 GELS_BOVIN
100.0 G Major actin P06396 GELS_HUMAN
96.9 A Major actin Q24733 ACT_DICVI
97.8 A Major actin P85911 ACT1_PSEMZ
97.6 A Major actin Q39596 ACT_OXYRB
90.4 A Major actin P10994 ACTS_PLEWA
95.5 A Major actin P00544 FGR_FSVGR
92.6 A Major actin P10982 ACT1_ABSGL
91.3 A Major actin Q25379 ACT3_LYTPI
90.1 A Major actin Q25381 ACTM_LYTPI
97.0 A Major actin Q11212 ACT_SPOLI
96.9 A Major actin P30170 ACT10_SOLTU
94.0 A Major actin Q92193 ACT_CRAVI
91.7 A Major actin Q92192 ACT_CALFI
92.6 A Major actin Q03342 ACT3_ECHGR
90.5 A Major actin P93585 ACT4_SOLTU
91.1 A Major actin P93376 ACT6_TOBAC
91.1 A Major actin P93587 ACT1_SOLTU
91.4 A Major actin Q96483 ACT2_SOLLC
92.0 A Major actin P93375 ACT7_TOBAC
91.4 A Major actin P93371 ACT5_TOBAC
91.4 A Major actin Q96484 ACT3_SOLLC
91.1 A Major actin Q96482 ACT1_SOLLC
94.5 A Major actin P18602 ACT3_ARTSX
92.0 A Major actin P93372 ACT4_TOBAC
92.0 A Major actin P93584 ACT9_SOLTU
92.0 A Major actin P81229 ACT8_SOLTU
92.3 A Major actin P81228 ACT5_SOLTU
92.2 A Major actin P30172 ACT12_SOLTU
90.1 A Major actin Q9Y701 ACT1_SUIBO
90.6 A Major actin P12431 ACTM_STRPU
90.3 A Major actin Q00214 ACTM_STYPL
90.4 A Major actin P30164 ACT1_PEA
90.3 A Major actin Q9Y702 ACT1_SCHCO
90.1 A Major actin P53467 ACTM_MOLOC
97.2 A Major actin Q55EU6 ACT23_DICDI
95.6 A Major actin P84856 ACTB_CHLPG
90.6 A Major actin P50138 ACT_PUCGR
91.2 A Major actin Q9Y707 ACT2_SUIBO
90.1 A Major actin P53504 ACT1_SORBI
91.4 A Major actin P20904 ACT_VOLCA
90.7 A Major actin P53498 ACT_CHLRE
91.4 A Major actin P53689 ACT_PHARH
91.5 A Major actin P26198 ACTM_STYCL
90.1 A Major actin P53494 ACT4_ARATH
91.2 A Major actin P53460 ACT1_HALRO
90.7 A Major actin P53479 ACTS_CYPCA
90.7 A Major actin P30173 ACT13_SOLTU
90.9 A Major actin Q90X97 ACTS_ATRMM
90.6 A Major actin P26197 ACT2_ABSGL
90.9 A Major actin P68264 ACTS_OREMO
90.9 A Major actin P68140 ACTSA_TAKRU
91.2 A Major actin P53482 ACTSB_TAKRU
91.8 A Major actin P53475 ACTN_STYCL
91.2 A Major actin P10995 ACT2_XENLA
91.2 A Major actin P68136 ACTS_RAT
91.2 A Major actin P68135 ACTS_RABIT
91.2 A Major actin Q5R9Q5 ACTS_PONAB
91.2 A Major actin P68137 ACTS_PIG
91.2 A Major actin Q98972 ACTS_ORYLA
91.2 A Major actin P68134 ACTS_MOUSE
91.2 A Major actin P68133 ACTS_HUMAN
91.2 A Major actin P68139 ACTS_CHICK
91.2 A Major actin P68138 ACTS_BOVIN
93.0 A Major actin P53474 ACTE_STRPU
91.2 A Major actin P49055 ACTS_CARAU
92.8 A Major actin P18499 ACTF_STRPU
91.5 A Major actin P53480 ACTC_TAKRU
91.2 A Major actin P53492 ACT7_ARATH
90.4 A Major actin P0CJ47 ACT3_ARATH
90.4 A Major actin P0CJ46 ACT1_ARATH
92.2 A Major actin O15998 ACTM_CIOSA
92.8 A Major actin Q00215 ACTC_STYPL
91.7 A Major actin P53459 ACT6_DIPDE
91.7 A Major actin Q6P8G3 ACT3_XENTR
91.7 A Major actin P04752 ACT3_XENLA
92.0 A Major actin P27130 ACT2_HALRO
92.8 A Major actin P53466 ACT2_LYTPI
90.7 A Major actin P30171 ACT11_SOLTU
92.0 A Major actin P04751 ACTC_XENLA
93.6 A Major actin Q07903 ACTC_STRPU
91.2 A Major actin Q10AZ4 ACT3_ORYSJ
91.2 A Major actin A2XNS1 ACT3_ORYSI
91.7 A Major actin P20399 ACT2_XENTR
90.9 A Major actin O81221 ACT_GOSHI
92.2 A Major actin P63269 ACTH_RAT
92.2 A Major actin P63268 ACTH_MOUSE
92.2 A Major actin P63267 ACTH_HUMAN
92.2 A Major actin P63270 ACTH_CHICK
92.2 A Major actin Q5E9B5 ACTH_BOVIN
92.0 A Major actin Q6P640 ACTC_XENTR
92.0 A Major actin P68035 ACTC_RAT
92.0 A Major actin P68033 ACTC_MOUSE
92.0 A Major actin P68032 ACTC_HUMAN
92.0 A Major actin P68034 ACTC_CHICK
92.0 A Major actin Q3ZC07 ACTC_BOVIN
92.2 A Major actin P08023 ACTA_CHICK
92.5 A Major actin P02574 ACT4_DROME
90.9 A Major actin P30167 ACT3_SOLTU
92.2 A Major actin Q25472 ACT2_MOLOC
90.9 A Major actin Q05214 ACT1_TOBAC
92.5 A Major actin P62738 ACTA_RAT
92.5 A Major actin P62740 ACTA_RABIT
92.5 A Major actin P62737 ACTA_MOUSE
92.5 A Major actin P62736 ACTA_HUMAN
92.5 A Major actin P62739 ACTA_BOVIN
92.8 A Major actin P45887 ACT5_BACDO
93.6 A Major actin P53465 ACT1_LYTPI
92.3 A Major actin O65315 ACT_COLSC
94.4 A Major actin P53473 ACTB_STRPU
91.5 A Major actin Q10DV7 ACT1_ORYSJ
91.5 A Major actin A2XLF2 ACT1_ORYSI
94.6 A Major actin P10990 ACT1_MESFR
91.7 A Major actin P53496 ACT11_ARATH
94.6 A Major actin P69005 ACTD_STRPU
94.6 A Major actin P69004 ACT2_MESFR
93.3 A Major actin P91754 ACT_LUMRU
93.3 A Major actin P12717 ACTM_PISOC
93.0 A Major actin P53457 ACT3_DIPDE
93.3 A Major actin P49128 ACT1_AEDAE
93.3 A Major actin P07837 ACT2_BOMMO
93.3 A Major actin P53463 ACTM_HELER
92.8 A Major actin P18600 ACT1_ARTSX
94.1 A Major actin P17304 ACTM_APLCA
94.6 A Major actin P53472 ACTA_STRPU
93.8 A Major actin P83968 ACT6_DROSI
93.8 A Major actin P83967 ACT6_DROME
93.8 A Major actin P83969 ACT1_BACDO
93.0 A Major actin O16808 ACT_MAYDE
93.3 A Major actin P53464 ACTM_HELTB
94.1 A Major actin P92176 ACT2_LUMTE
93.3 A Major actin P18601 ACT2_ARTSX
94.3 A Major actin P53458 ACT5_DIPDE
94.9 A Major actin P69003 ACT1_HELTB
94.9 A Major actin P69002 ACT1_HELER
94.1 A Major actin P41341 ACTY_LIMPO
93.3 A Major actin P10981 ACT5_DROME
94.1 A Major actin O18500 ACT2_SACKO
93.3 A Major actin P53501 ACT3_DROME
93.6 A Major actin P53456 ACT2_DIPDE
93.6 A Major actin O17503 ACTC_BRALA
93.6 A Major actin P92182 ACT1_LUMTE
93.6 A Major actin P45885 ACT2_BACDO
94.1 A Major actin P41113 ACT3_PODCA
93.6 A Major actin P45886 ACT3_BACDO
93.8 A Major actin P68555 ACT_TAESO
94.9 A Major actin P41112 ACT1_PODCA
93.8 A Major actin P68556 ACT1_DIPDE
93.8 A Major actin P07836 ACT1_BOMMO
94.1 A Major actin P49871 ACT_MANSE
94.6 A Major actin P41339 ACTA_LIMPO
94.4 A Major actin P17126 ACT_HYDVU
94.9 A Major actin P84336 ACTB_CAMDR
94.9 A Major actin P90689 ACT_BRUMA
94.4 A Major actin Q964D9 ACTC_PLATR
94.4 A Major actin Q964E0 ACTC_BIOTE
94.6 A Major actin P92179 ACTC_BIOGL
94.6 A Major actin Q0PGG4 ACTB_BOSMU
94.9 A Major actin P53461 ACTC_HALRO
94.4 A Major actin P53485 ACTB2_TAKRU
95.2 A Major actin P63256 ACTG_ANSAN
94.6 A Major actin Q964E3 ACTC_BIOAL
94.9 A Major actin P53470 ACT1_SCHMA
94.4 A Major actin O18499 ACT1_SACKO
94.9 A Major actin Q964E2 ACTC_BIOPF
94.9 A Major actin Q964E1 ACTC_BIOOB
94.6 A Major actin P83750 ACTB_CYPCA
94.6 A Major actin P83751 ACTB_CTEID
94.6 A Major actin Q7ZVF9 ACTB2_DANRE
94.4 A Major actin O17320 ACT_CRAGI
95.2 A Major actin Q26065 ACT_PLAMG
95.2 A Major actin P29751 ACTB_RABIT
94.9 A Major actin Q7ZVI7 ACTB1_DANRE
94.9 A Major actin O42161 ACTB_SALSA
95.4 A Major actin P53506 ACT8_XENLA
94.4 A Major actin P0DM42 ACT3_CAEEL
94.4 A Major actin P0DM41 ACT1_CAEEL
95.4 A Major actin P04829 ACT3_BOMMO
95.4 A Major actin P15475 ACTB_XENBO
94.9 A Major actin P79818 ACTB_ORYLA
94.6 A Major actin P10986 ACT4_CAEEL
95.2 A Major actin Q8JJB8 ACTG_TRISC
94.9 A Major actin Q93131 ACTC_BRAFL
95.2 A Major actin Q91ZK5 ACTB_SIGHI
95.2 A Major actin P68143 ACTB_OREMO
95.2 A Major actin P53486 ACTB3_TAKRU
95.2 A Major actin P68142 ACTB1_TAKRU
95.2 A Major actin P30163 ACT2_ONCVO
95.2 A Major actin P48975 ACTB_CRIGR
95.2 A Major actin P53478 ACT5_CHICK
95.2 A Major actin P02572 ACT2_DROME
94.9 A Major actin P30162 ACT1_ONCVO
96.0 A Major actin P12716 ACTC_PISOC
95.4 A Major actin P18603 ACT4_ARTSX
95.2 A Major actin P41340 ACT3_LIMPO
95.4 A Major actin Q6P378 ACTG_XENTR
95.2 A Major actin Q93129 ACTC_BRABE
95.7 A Major actin Q25010 ACT3A_HELAM
95.7 A Major actin A2BDB0 ACTG_XENLA
95.7 A Major actin P63257 ACTG_TRIVU
95.7 A Major actin P63259 ACTG_RAT
95.7 A Major actin Q5JAK2 ACTG_PELLE
95.7 A Major actin P63260 ACTG_MOUSE
95.7 A Major actin P63261 ACTG_HUMAN
95.7 A Major actin Q5ZMQ2 ACTG_CHICK
95.7 A Major actin P63258 ACTG_BOVIN
95.4 A Major actin P60707 ACTB_TRIVU
95.4 A Major actin Q4L0Y2 ACTB_SPECI
95.4 A Major actin P60713 ACTB_SHEEP
95.4 A Major actin P60711 ACTB_RAT
95.4 A Major actin Q5R6G0 ACTB_PONAB
95.4 A Major actin Q6QAQ1 ACTB_PIG
95.4 A Major actin Q5R1X3 ACTB_PANTR
95.4 A Major actin P60710 ACTB_MOUSE
95.4 A Major actin Q711N9 ACTB_MESAU
95.4 A Major actin Q4R561 ACTB_MACFA
95.4 A Major actin P60709 ACTB_HUMAN
95.4 A Major actin P60708 ACTB_HORSE
95.4 A Major actin Q76N69 ACTB_CHLAE
95.4 A Major actin P60706 ACTB_CHICK
95.4 A Major actin Q71FK5 ACTB_CAVPO
95.4 A Major actin O18840 ACTB_CANLF
95.4 A Major actin P60712 ACTB_BOVIN
95.7 A Major actin P53505 ACT5_XENLA
95.2 A Major actin P53471 ACT2_SCHMA
95.2 A Major actin P10984 ACT2_CAEEL
95.7 A Major actin P84185 ACT5C_ANOGA
95.7 A Major actin P84183 ACT4_BOMMO
95.7 A Major actin P84184 ACT3B_HELAM
95.7 A Major actin P10987 ACT1_DROME
95.7 A Major actin Q6NVA9 ACTB_XENTR
95.7 A Major actin O93400 ACTB_XENLA
96.5 A Major actin P07829 ACT3_DICDI
97.6 A Major actin P02578 ACT1_ACACA
98.4 A Major actin Q553U6 ACT22_DICDI
98.9 A Major actin P02576 ACTA_PHYPO
99.2 A Major actin Q54GX7 ACT10_DICDI
99.5 A Major actin P07830 ACT1_DICDI