Equilibria and kinetics of folding of gelsolin domain 2 and mutants involved in familial amyloidosis-Finnish type.
Abstract
Mutations D187N and D187Y in domain 2 of the actin-regulating protein gelsolin cause familial amyloidosis-Finnish type (FAF). We have constructed and expressed a recombinant version of gelsolin domain 2 that is sufficiently stable for kinetic and equilibrium measurements. The wild-type domain and the two amyloidogenic mutants fold via simple two-state kinetics without the accumulation of an intermediate. Unfolding kinetics exhibits significant curvature with increasing urea concentration, indicating that the transition state for unfolding becomes more native-like under increasingly denaturing conditions in accordance with the Hammond postulate. Mutations D187N and D187Y destabilize gelsolin domain 2 by 1.22 and 2.16 kcal. mol(-1) (1 kcal = 4.18 kJ) respectively. The mutations do not prevent disulfide bond formation despite their direct contiguity with a cysteine residue involved in disulfide linkage. The destabilization conferred on gelsolin domain 2 by the FAF mutations is sufficient to predict that an appreciable fraction is unfolded and, therefore, extremely susceptible to proteolysis at body temperature. Study holds ProTherm entries: 6490, 6491, 6492, 6493 Extra Details: protein; stability; denaturation; Hammond; two-state
Submission Details
ID: ezWdA95J
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:32 p.m.
Version: 1
Publication Details
Isaacson RL;Weeds AG;Fersht AR,Proc. Natl. Acad. Sci. U.S.A. (1999) Equilibria and kinetics of folding of gelsolin domain 2 and mutants involved in familial amyloidosis-Finnish type. PMID:10500162Additional Information
Study Summary
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
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Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant PDB Entries
| Structure ID | Release Date | Resolution | Structure Title |
|---|---|---|---|
| 3B5U | 2008-04-15 | Actin filament model from extended form of acromsomal bundle in the Limulus sperm | |
| 2W4U | 2010-08-25 | Isometrically contracting insect asynchronous flight muscle quick frozen after a length step | |
| 5H3M | 2017-11-01 | Solution structure of human Gelsolin protein domain 1 at pH 5.0 | |
| 5H3N | 2017-11-01 | Solution structure of human Gelsolin protein domain 1 at pH 7.3 | |
| 1SOL | 1996-03-08 | A PIP2 AND F-ACTIN-BINDING SITE OF GELSOLIN, RESIDUE 150-169 (NMR, AVERAGED STRUCTURE) | |
| 2W49 | 2010-05-05 | ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE | |
| 5FAF | 2016-10-05 | 1.05 | N184K pathological variant of gelsolin domain 2 (orthorhombic form) |
| 6I4E | 2019-06-26 | 1.22 | Crystal Structure of Plasmodium falciparum actin I in the Mg-ADP state |
| 6I4D | 2019-06-26 | 1.24 | Crystal Structure of Plasmodium falciparum actin I in the Mg-K-ATP/ADP state |
| 4B1Y | 2013-07-31 | 1.29 | Structure of the Phactr1 RPEL-3 bound to G-actin |
| 6QW3 | 2019-08-28 | 1.3 | Calcium-bound gelsolin domain 2 |
| 4CBU | 2014-04-30 | 1.3 | Crystal structure of Plasmodium falciparum actin I |
| 2FXU | 2006-03-07 | 1.35 | X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution. |
| 6LJE | 2020-01-01 | 1.4 | Crystal structure of gelsolin G3 domain (calcium and magnesium condition) |
| 5MVV | 2017-07-12 | 1.4 | Crystal structure of Plasmodium falciparum actin I- gelsolin segment 1 -CdATP complex |
| 6I4H | 2019-06-26 | 1.4 | Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Ca-ATP state |
| 4K41 | 2014-10-01 | 1.4 | Crystal structure of actin in complex with marine macrolide kabiramide C |
| 2Q0U | 2007-07-17 | 1.45 | Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin |
| 1WUA | 2006-02-14 | 1.45 | The structure of Aplyronine A-actin complex |
| 2V52 | 2008-11-25 | 1.45 | Structure of MAL-RPEL2 complexed to G-actin |
| 1QZ5 | 2003-11-11 | 1.45 | Structure of rabbit actin in complex with kabiramide C |
| 6I4J | 2019-06-26 | 1.5 | Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Mg-ADP state |
| 6LJF | 2020-01-01 | 1.5 | Crystal structure of gelsolin G3 domain (calcium condition) |
| 2PBD | 2007-11-13 | 1.5 | Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP* |
| 3MN5 | 2010-06-02 | 1.5 | Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation |
| 6I4F | 2019-06-26 | 1.5 | Crystal Structure of Plasmodium falciparum actin I (A272W mutant) in the Mg-K-ATP/ADP state |
| 5ZZA | 2018-10-10 | 1.53 | OdinProfilin/Rabbit Actin Complex |
| 1J6Z | 2001-08-15 | 1.54 | UNCOMPLEXED ACTIN |
| 2FH1 | 2006-06-13 | 1.55 | C-terminal half of gelsolin soaked in low calcium at pH 4.5 |
| 3CIP | 2008-08-19 | 1.6 | Complex of Dictyostelium Discoideum Actin with Gelsolin |
| 2ASM | 2005-10-11 | 1.6 | Structure of Rabbit Actin In Complex With Reidispongiolide A |
| 1S22 | 2004-02-17 | 1.6 | Absolute Stereochemistry of Ulapualide A |
| 1QZ6 | 2003-11-11 | 1.6 | Structure of rabbit actin in complex with jaspisamide A |
| 2ASP | 2005-10-11 | 1.64 | Structure of Rabbit Actin In Complex With Reidispongiolide C |
| 1KCQ | 2002-01-04 | 1.65 | Human Gelsolin Domain 2 with a Cd2+ bound |
| 6MBK | 2018-12-19 | 1.69 | SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, First P212121 Crystal Form |
| 3CI5 | 2008-08-19 | 1.7 | Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin |
| 5FAE | 2016-10-05 | 1.7 | N184K pathological variant of gelsolin domain 2 (trigonal form) |
| 2Q0R | 2007-07-17 | 1.7 | Structure of Pectenotoxin-2 Bound to Actin |
| 5O2Z | 2017-11-08 | 1.7 | Domain swap dimer of the G167R variant of gelsolin second domain |
| 2ASO | 2005-10-11 | 1.7 | Structure of Rabbit Actin In Complex With Sphinxolide B |
| 6W7V | 2020-10-21 | 1.7 | Structure of rabbit actin in complex with truncated analog of Mycalolide B |
| 7C2G | 2020-08-05 | 1.71 | Crystal Structure of the Thorarchaeaota 2DGel/rabbit actin complex |
| 6JH9 | 2020-02-19 | 1.74 | Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. Lys18Arg |
| 1D4X | 2001-05-02 | 1.75 | Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin Segment 1 at 1.75 A resolution. |
| 3SJH | 2012-01-25 | 1.75 | Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP-Latrunculin A |
| 4H03 | 2013-02-20 | 1.75 | Crystal structure of NAD+-Ia-actin complex |
| 6OX0 | 2019-08-21 | 1.75 | SETD3 in Complex with an Actin Peptide with Sinefungin Replacing SAH as Cofactor |
| 4B1V | 2012-11-07 | 1.75 | Structure of the Phactr1 RPEL-N domain bound to G-actin |
| 6WK2 | 2020-06-17 | 1.76 | SETD3 mutant (N255V) in Complex with an Actin Peptide with His73 Replaced with Methionine |
| 6MBJ | 2018-12-19 | 1.78 | SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, P21 Crystal Form |
| 6OX3 | 2019-08-21 | 1.78 | SETD3 in Complex with an Actin Peptide with His73 Replaced with Lysine |
| 1NM1 | 2003-01-21 | 1.8 | Crystal Structure of D. Dicsoideum Actin Complexed With Gelsolin Segment 1 and Mg ATP at 1.8 A Resolution |
| 3EKS | 2008-10-07 | 1.8 | Crystal Structure of Monomeric Actin bound to Cytochalasin D |
| 2HF4 | 2006-08-29 | 1.8 | Crystal structure of Monomeric Actin in its ATP-bound state |
| 4PKG | 2014-07-30 | 1.8 | Complex of ATP-actin With the N-terminal Actin-Binding Domain of Tropomodulin |
| 2A40 | 2005-11-01 | 1.8 | Ternary complex of the WH2 domain of WAVE with Actin-DNAse I |
| 4B1X | 2013-07-31 | 1.8 | Structure of the Phactr1 RPEL-2 bound to G-actin |
| 2HF3 | 2006-08-29 | 1.8 | Crystal structure of monomeric Actin in the ADP bound state |
| 3W3D | 2013-01-30 | 1.8 | Crystal structure of smooth muscle G actin DNase I complex |
| 1NLV | 2003-01-21 | 1.8 | Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human Gelsolin Segment 1 |
| 2PAV | 2007-10-23 | 1.8 | Ternary complex of Profilin-Actin with the Last Poly-Pro of Human VASP |
| 5YEE | 2018-09-19 | 1.81 | Crystal structure of LokiProfilin1/Rabbit Actin Complex |
| 6I4K | 2019-06-26 | 1.83 | Crystal Structure of Plasmodium falciparum actin I (G115A mutant) in the Ca-ATP state |
| 6I4L | 2019-06-26 | 1.83 | Crystal Structure of Plasmodium falciparum actin I (G115A mutant) in the Mg-K-ATP/ADP state |
| 1NWK | 2003-10-14 | 1.85 | CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE |
| 2A42 | 2005-11-01 | 1.85 | Actin-DNAse I Complex |
| 6I4M | 2019-06-26 | 1.87 | Crystal Structure of Plasmodium berghei actin II in the Mg-ADP state |
| 6WK1 | 2020-06-17 | 1.89 | SETD3 in Complex with an Actin Peptide with His73 Replaced with Methionine |
| 6I4I | 2019-06-26 | 1.9 | Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Mg-K-ADP-AlFn state |
| 1NMD | 2003-02-04 | 1.9 | Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP |
| 1YAG | 1999-10-09 | 1.9 | STRUCTURE OF THE YEAST ACTIN-HUMAN GELSOLIN SEGMENT 1 COMPLEX |
| 2GWJ | 2006-08-29 | 1.9 | SpvB ADP-ribosylated actin: hexagonal crystal form |
| 2HMP | 2006-09-19 | 1.9 | Uncomplexed actin cleaved with protease ECP32 |
| 6H1F | 2019-01-23 | 1.9 | Structure of the nanobody-stabilized gelsolin D187N variant (second domain) |
| 4H0Y | 2013-02-20 | 1.94 | Crystal structure of NAD+-Ia(E380S)-actin complex |
| 6OX1 | 2019-08-21 | 1.95 | SETD3 in Complex with an Actin Peptide with Target Histidine Partially Methylated |
| 6RSW | 2019-11-27 | 1.95 | HFD domain of mouse CAP1 bound to the pointed end of G-actin |
| 6ICT | 2019-02-27 | 1.95 | Structure of SETD3 bound to SAH and methylated actin |
| 4B1W | 2013-07-31 | 1.95 | Structure of the Phactr1 RPEL-2 domain bound to actin |
| 5NOJ | 2017-08-02 | 11.0 | Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - 'OPEN' state |
| 5NOG | 2017-07-19 | 11.0 | Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - 'Blocked' state |
| 6CXJ | 2018-10-10 | 11.0 | Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 2 |
| 6CXI | 2018-10-10 | 11.0 | Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 1 |
| 3BYH | 2008-02-19 | 12.0 | Model of actin-fimbrin ABD2 complex |
| 3J8J | 2015-01-14 | 12.0 | Tilted state of actin, T1 |
| 3J8K | 2015-01-14 | 12.0 | Tilted state of actin, T2 |
| 3LUE | 2010-04-28 | 15.0 | Model of alpha-actinin CH1 bound to F-actin |
| 2FF3 | 2006-03-21 | 2.0 | Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin |
| 3U8X | 2012-01-25 | 2.0 | Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP |
| 1C0G | 2000-03-01 | 2.0 | CRYSTAL STRUCTURE OF 1:1 COMPLEX BETWEEN GELSOLIN SEGMENT 1 AND A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 228: Q228K/T229A/A230Y/E360H) |
| 6NBE | 2020-04-15 | 2.0 | Ternary Complex of Ac-Alpha-Actin with Profilin and CoA-NAA80 |
| 3MN7 | 2010-05-26 | 2.0 | Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation |
| 4PL8 | 2014-10-22 | 2.0 | Structure of rabbit skeletal muscle actin in complex with a hybrid peptide comprising thymosin beta4 and the lysine-rich region of Cordon-Bleu |
| 1ESV | 2000-07-19 | 2.0 | COMPLEX BETWEEN LATRUNCULIN A:RABBIT MUSCLE ALPHA ACTIN:HUMAN GELSOLIN DOMAIN 1 |
| 3M6G | 2010-09-08 | 2.0 | Crystal structure of actin in complex with lobophorolide |
| 3MN6 | 2010-06-02 | 2.0 | Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation |
| 1T44 | 2004-09-07 | 2.0 | Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation |
| 3MN9 | 2010-05-26 | 2.0 | Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation |
| 4B1U | 2013-07-31 | 2.0 | Structure of the Phactr1 RPEL domain and RPEL motif directed assemblies with G-actin reveal the molecular basis for actin binding cooperativity. |
| 5YPU | 2018-09-26 | 2.0 | Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu MET72NLE WH2-motif peptide |
| 6I4G | 2019-06-26 | 2.0 | Crystal Structure of Plasmodium falciparum actin I (H74Q) in the Mg-K-ATP state |
| 1P8X | 2003-10-14 | 2.0 | The Calcium-Activated C-terminal half of gelsolin |
| 2GWK | 2006-08-29 | 2.0 | SpvB ADP-ribosylated actin: orthorhombic crystal form |
| 1YXQ | 2005-05-17 | 2.01 | Crystal structure of actin in complex with swinholide A |
| 6V63 | 2020-01-22 | 2.02 | SETD3 WT in Complex with an Actin Peptide with His73 Replaced with Glutamine |
| 4H0V | 2013-02-20 | 2.03 | Crystal structure of NAD+-Ia(E378S)-actin complex |
| 7C2F | 2020-08-05 | 2.03 | Crystal Structure of the Thorarchaeaota ProGel/rabbit actin complex |
| 2FF6 | 2006-03-21 | 2.05 | Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin |
| 2A3Z | 2005-11-01 | 2.08 | Ternary complex of the WH2 domain of WASP with Actin-DNAse I |
| 6OX2 | 2019-08-21 | 2.09 | SETD3in Complex with an Actin Peptide with the Target Histidine Fully Methylated |
| 3U9Z | 2012-01-25 | 2.09 | Crystal structure between actin and a protein construct containing the first beta-thymosin domain of drosophila ciboulot (residues 2-58) with the three mutations N26D/Q27K/D28S |
| 1YVN | 2000-03-23 | 2.1 | THE YEAST ACTIN VAL 159 ASN MUTANT COMPLEX WITH HUMAN GELSOLIN SEGMENT 1. |
| 6OX5 | 2019-08-21 | 2.1 | A SETD3 Mutant (N255A) in Complex with an Actin Peptide with His73 Replaced with Lysine |
| 1KXP | 2002-06-19 | 2.1 | CRYSTAL STRUCTURE OF HUMAN VITAMIN D-BINDING PROTEIN IN COMPLEX WITH SKELETAL ACTIN |
| 6ICV | 2019-02-27 | 2.15 | Structure of SETD3 bound to SAH and unmodified actin |
| 6JH8 | 2020-02-19 | 2.15 | Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. |
| 4PKH | 2014-07-30 | 2.15 | Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin |
| 6MGO | 2018-11-21 | 2.2 | Structure of rabbit actin in complex with Mycalolide B |
| 3UB5 | 2012-04-25 | 2.2 | Profilin:actin with a wide open nucleotide cleft |
| 6MBL | 2018-12-19 | 2.2 | SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, Second P212121 Crystal Form |
| 4H0T | 2013-02-20 | 2.2 | Crystal structure of Ia-ADPR-actin complex |
| 1MDU | 2003-01-07 | 2.2 | Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1 (GS-1) |
| 4CBX | 2014-04-30 | 2.2 | Crystal structure of Plasmodium berghei actin II |
| 3D2U | 2008-07-08 | 2.21 | Structure of UL18, a Peptide-Binding Viral MHC Mimic, Bound to a Host Inhibitory Receptor |
| 6OX4 | 2019-08-21 | 2.29 | A SETD3 Mutant (N255A) in Complex with an Actin Peptide |
| 1RDW | 2003-12-16 | 2.3 | Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer |
| 6JCU | 2020-02-05 | 2.3 | Crystal structure of an actin monomer in complex with a nucleator Cordon-Bleu WH2-motif peptide mutant. T22V, H11R |
| 5ZZB | 2018-10-10 | 2.3 | LokiProfilin2/Rabbit Actin Complex |
| 3CHW | 2008-08-19 | 2.3 | Complex of Dictyostelium discoideum Actin with Profilin and the Last Poly-Pro of Human VASP |
| 1EQY | 2000-05-03 | 2.3 | COMPLEX BETWEEN RABBIT MUSCLE ALPHA-ACTIN: HUMAN GELSOLIN DOMAIN 1 |
| 4PKI | 2014-07-30 | 2.3 | Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin |
| 4H0X | 2013-02-20 | 2.33 | Crystal structure of NAD+-Ia(E380A)-actin complex |
| 2VYP | 2009-02-24 | 2.35 | Rabbit-muscle G-actin in complex with myxobacterial rhizopodin |
| 7C2H | 2020-08-05 | 2.35 | Crystal Structure of the Thorarchaeaota 2DGel3/rabbit actin complex |
| 2V51 | 2008-11-25 | 2.35 | Structure of MAL-RPEL1 complexed to actin |
| 6V62 | 2020-01-15 | 2.36 | SETD3 double mutant (N255F/W273A) in Complex with an Actin Peptide with His73 Replaced with Lysine |
| 3A5N | 2010-10-27 | 2.36 | Crystal Structure of a Dictyostelium P109A Ca2+-Actin in Complex with Human Gelsolin Segment 1 |
| 5UBO | 2017-12-20 | 2.39 | Mical-oxidized Actin complex with Gelsolin Segment 1 |
| 1C0F | 2000-03-01 | 2.4 | CRYSTAL STRUCTURE OF DICTYOSTELIUM CAATP-ACTIN IN COMPLEX WITH GELSOLIN SEGMENT 1 |
| 3A5O | 2010-10-27 | 2.4 | Crystal Structure of a Dictyostelium P109I Ca2+-Actin in Complex with Human Gelsolin Segment 1 |
| 3A5L | 2010-10-27 | 2.4 | Crystal Structure of a Dictyostelium P109A Mg2+-Actin in Complex with Human Gelsolin Segment 1 |
| 3U4L | 2012-04-25 | 2.4 | Cryocooled bovine profilin:actin crystal structure to 2.4 A |
| 1DEJ | 2000-03-01 | 2.4 | CRYSTAL STRUCTURE OF A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 646: Q228K/T229A/A230Y/A231K/S232E/E360H) IN COMPLEX WITH HUMAN GELSOLIN SEGMENT 1 |
| 3A5M | 2010-10-27 | 2.4 | Crystal Structure of a Dictyostelium P109I Mg2+-Actin in Complex with Human Gelsolin Segment 1 |
| 4Z94 | 2015-10-21 | 2.4 | Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2 |
| 6QRI | 2019-07-03 | 2.4 | Structure of rabbit G-actin in complex with chivosazole A |
| 1MA9 | 2003-02-04 | 2.4 | Crystal structure of the complex of human vitamin D binding protein and rabbit muscle actin |
| 6JBK | 2020-02-05 | 2.45 | Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V |
| 4EFH | 2012-04-11 | 2.48 | Acanthamoeba Actin complex with Spir domain D |
| 2A5X | 2005-08-23 | 2.49 | Crystal Structure of a Cross-linked Actin Dimer |
| 1SQK | 2004-06-15 | 2.5 | CRYSTAL STRUCTURE OF CIBOULOT IN COMPLEX WITH SKELETAL ACTIN |
| 2FH2 | 2006-06-13 | 2.5 | C-terminal half of gelsolin soaked in EGTA at pH 4.5 |
| 1LOT | 2002-07-31 | 2.5 | CRYSTAL STRUCTURE OF THE COMPLEX OF ACTIN WITH VITAMIN D-BINDING PROTEIN |
| 1D0N | 1999-09-15 | 2.5 | THE CRYSTAL STRUCTURE OF CALCIUM-FREE EQUINE PLASMA GELSOLIN. |
| 3U9D | 2012-01-25 | 2.5 | Crystal Structure of a chimera containing the N-terminal domain (residues 8-24) of drosophila Ciboulot and the C-terminal domain (residues 13-44) of bovine Thymosin-beta4, bound to G-actin-ATP |
| 3EKU | 2008-10-07 | 2.5 | Crystal Structure of Monomeric Actin bound to Cytochalasin D |
| 2Q97 | 2007-10-16 | 2.5 | Complex of mammalian actin with toxofilin from toxoplasma gondii |
| 2Q36 | 2007-06-05 | 2.5 | Actin Dimer Cross-linked between Residues 191 and 374 and complexed with Kabiramide C |
| 6NBW | 2020-01-29 | 2.5 | Ternary Complex of Beta/Gamma-Actin with Profilin and AnCoA-NAA80 |
| 4CBW | 2014-04-30 | 2.5 | Crystal structure of Plasmodium berghei actin I with D-loop from muscle actin |
| 3EL2 | 2008-10-07 | 2.5 | Crystal Structure of Monomeric Actin Bound to Ca-ATP |
| 2D1K | 2006-09-12 | 2.5 | Ternary complex of the WH2 domain of mim with actin-dnase I |
| 2BTF | 1994-06-22 | 2.55 | THE STRUCTURE OF CRYSTALLINE PROFILIN-BETA-ACTIN |
| 3DAW | 2008-07-29 | 2.55 | Structure of the actin-depolymerizing factor homology domain in complex with actin |
| 5DD2 | 2016-10-05 | 2.6 | Human gelsolin from residues Glu28 to Arg161 with Calcium |
| 1P8Z | 2003-10-14 | 2.6 | Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156 |
| 2A41 | 2005-11-01 | 2.6 | Ternary complex of the WH2 Domain of WIP with Actin-DNAse I |
| 6GVC | 2019-03-27 | 2.6 | Structure of ArhGAP12 bound to G-Actin |
| 4S10 | 2015-06-03 | 2.61 | Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis |
| 2OAN | 2007-05-01 | 2.61 | Structure of oxidized beta-actin |
| 5ZZ0 | 2019-06-05 | 2.63 | HUMAN GELSOLIN FROM RESIDUES GLU28 TO ARG161 WITH CALCIUM |
| 1HLU | 1997-10-15 | 2.65 | STRUCTURE OF BOVINE BETA-ACTIN-PROFILIN COMPLEX WITH ACTIN BOUND ATP PHOSPHATES SOLVENT ACCESSIBLE |
| 2Q31 | 2007-06-05 | 2.7 | Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48. |
| 3HBT | 2010-05-05 | 2.7 | The structure of native G-actin |
| 2Q1N | 2007-06-05 | 2.7 | Actin Dimer Cross-linked Between Residues 41 and 374 |
| 4GY2 | 2013-02-20 | 2.71 | Crystal structure of apo-Ia-actin complex |
| 6JAT | 2020-01-29 | 2.71 | Crystal structure of SETD3 bound to Actin peptide and SFG |
| 6Q9R | 2019-11-27 | 2.73 | Crystal structure of the pathological N184K variant of calcium-free human gelsolin |
| 4M63 | 2013-10-23 | 2.75 | Crystal Structure of a Filament-Like Actin Trimer Bound to the Bacterial Effector VopL |
| 3UE5 | 2012-02-15 | 2.76 | ECP-cleaved Actin in complex with Spir domain D |
| 1ATN | 1992-07-15 | 2.8 | Atomic structure of the actin:DNASE I complex |
| 6FM2 | 2018-05-16 | 2.8 | CARP domain of mouse cyclase-associated protein 1 (CAP1) bound to ADP-actin |
| 3MMV | 2010-06-02 | 2.8 | Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation |
| 2FGH | 2006-04-18 | 2.8 | ATP bound gelsolin |
| 3BUZ | 2008-05-13 | 2.81 | Crystal structure of ia-bTAD-actin complex |
| 1IJJ | 2002-04-15 | 2.85 | THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION |
| 2FH3 | 2006-06-13 | 2.87 | C-terminal half of gelsolin soaked in low calcium at pH 8 |
| 6JCO | 2019-06-12 | 2.88 | Crystal structure of calcium free human gelsolin amyloid mutant D187N |
| 3M1F | 2010-09-15 | 2.89 | Crosslinked complex of actin with first W domain of Vibrio parahaemolyticus VopL |
| 6NAS | 2020-01-29 | 2.9 | Ternary Complex of Ac-Alpha-Actin with Profilin and AcCoA-NAA80 |
| 6UPW | 2020-09-30 | 2.9 | Metavinculin ABD-F-actin complex |
| 4K43 | 2014-10-01 | 2.9 | Crystal structure of actin in complex with synthetic AplC tail analogue GC04 [N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide] |
| 4K42 | 2014-10-01 | 2.9 | Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate] |
| 4JHD | 2013-06-19 | 2.91 | Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu |
| 6JEH | 2019-06-12 | 2.95 | Crystal structure of calcium free human gelsolin amyloid mutant D187Y |
| 6JEG | 2019-06-12 | 2.97 | Crystal structure of calcium free human gelsolin amyloid mutant G167R |
| 4RWT | 2015-10-14 | 2.98 | Structure of actin-Lmod complex |
| 1H1V | 2003-01-24 | 2.99 | gelsolin G4-G6/actin complex |
| 2Y83 | 2011-03-30 | 22.9 | Actin filament pointed end |
| 5MVA | 2017-11-29 | 27.7 | Structure of the thin filament at high calcium concentration |
| 5MVY | 2018-02-14 | 28.4 | Thin Filament at low calcium concentration |
| 1NPH | 2003-05-13 | 3.0 | Gelsolin Domains 4-6 in Active, Actin Free Conformation Identifies Sites of Regulatory Calcium Ions |
| 6KLL | 2020-01-15 | 3.0 | F-actin of cardiac thin filament in low-calcium state |
| 5WFN | 2017-08-30 | 3.0 | Revised model of leiomodin 2-mediated actin regulation (alternate refinement of PDB 4RWT) |
| 3FFN | 2009-10-06 | 3.0 | Crystal structure of calcium-free human gelsolin |
| 3FFK | 2009-10-06 | 3.0 | Crystal structure of human Gelsolin domains G1-G3 bound to Actin |
| 1RFQ | 2003-12-16 | 3.0 | Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer |
| 3TU5 | 2012-09-26 | 3.0 | Actin complex with Gelsolin Segment 1 fused to Cobl segment |
| 2FH4 | 2006-06-13 | 3.0 | C-terminal half of gelsolin soaked in EGTA at pH 8 |
| 7K21 | 2020-11-04 | 3.0 | Cryo-EM structure of pyrene-labeled ADP-Pi-actin filaments |
| 1RGI | 2004-07-27 | 3.0 | Crystal structure of gelsolin domains G1-G3 bound to actin |
| 1Y64 | 2005-01-18 | 3.05 | Bni1p Formin Homology 2 Domain complexed with ATP-actin |
| 6DJN | 2019-02-27 | 3.1 | Cryo-EM structure of ADP-Pi-actin filaments |
| 6DJM | 2019-02-27 | 3.1 | Cryo-EM structure of AMPPNP-actin filaments |
| 6T23 | 2020-03-04 | 3.1 | Cryo-EM structure of jasplakinolide-stabilized F-actin (aged) |
| 2YJE | 2011-07-06 | 3.1 | Oligomeric assembly of actin bound to MRTF-A |
| 6C1D | 2018-01-31 | 3.2 | High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing |
| 7K20 | 2020-11-04 | 3.2 | Cryo-EM structure of pyrene-labeled ADP-actin filaments |
| 6UPV | 2020-09-30 | 3.2 | Alpha-E-catenin ABD-F-actin complex |
| 2VCP | 2008-11-04 | 3.2 | Crystal structure of N-Wasp VC domain in complex with skeletal actin |
| 3CJB | 2008-03-25 | 3.21 | Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1 |
| 6FHL | 2018-06-13 | 3.3 | Cryo-EM structure of F-actin in complex with ADP-Pi |
| 4B1Z | 2012-11-07 | 3.3 | Structure of the Phactr1 RPEL domain bound to G-actin |
| 2ZWH | 2009-01-20 | 3.3 | Model for the F-actin structure |
| 6T1Y | 2020-03-04 | 3.3 | Cryo-EM structure of phalloidin-stabilized F-actin (copolymerized) |
| 6KLN | 2020-01-15 | 3.4 | F-actin of cardiac thin filament in high-calcium state |
| 4EAH | 2012-12-12 | 3.4 | Crystal structure of the formin homology 2 domain of FMNL3 bound to actin |
| 5OOF | 2018-06-13 | 3.4 | Cryo-EM structure of F-actin in complex with ADP-BeFx |
| 6VAO | 2020-01-08 | 3.4 | Human cofilin-1 decorated actin filament |
| 3TPQ | 2011-10-12 | 3.45 | Crystal structure of wild-type MAL RPEL domain in complex with five G-actins |
| 4WYB | 2015-08-19 | 3.49 | Structure of the Bud6 flank domain in complex with actin |
| 6QBF | 2019-11-27 | 3.5 | Crystal structure of the pathological D187N variant of calcium-free human gelsolin. |
| 7AD9 | 2020-10-28 | 3.5 | Structure of the Lifeact-F-actin complex |
| 1LCU | 2002-05-01 | 3.5 | Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution |
| 2YJF | 2011-07-06 | 3.5 | Oligomeric assembly of actin bound to MRTF-A |
| 6F1T | 2018-01-17 | 3.5 | Cryo-EM structure of two dynein tail domains bound to dynactin and BICDR1 |
| 6VAU | 2020-01-01 | 3.5 | Bare actin filament from a partially cofilin-decorated sample |
| 6D8C | 2018-09-19 | 3.54 | Cryo-EM structure of FLNaABD E254K bound to phalloidin-stabilized F-actin |
| 6WVT | 2020-10-07 | 3.56 | Structural basis of alphaE-catenin - F-actin catch bond behavior |
| 6M5G | 2020-05-20 | 3.6 | F-actin-Utrophin complex |
| 5OOE | 2018-06-13 | 3.6 | Cryo-EM structure of F-actin in complex with AppNHp (AMPPNP) |
| 7BTI | 2020-05-20 | 3.6 | Phalloidin bound F-actin complex |
| 5JLF | 2016-06-15 | 3.6 | Structure of the F-actin-tropomyosin complex (Reprocessed) |
| 6T25 | 2020-03-04 | 3.6 | Cryo-EM structure of phalloidin-Alexa Flour-546-stabilized F-actin (copolymerized) |
| 5OOC | 2018-06-13 | 3.6 | Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP |
| 6DJO | 2019-02-27 | 3.6 | Cryo-EM structure of ADP-actin filaments |
| 6T24 | 2020-03-04 | 3.7 | Cryo-EM structure of jasplakinolide-stabilized F-actin (aged) |
| 6T20 | 2020-03-04 | 3.7 | Cryo-EM structure of phalloidin-stabilized F-actin (aged) |
| 6LTJ | 2020-02-12 | 3.7 | Structure of nucleosome-bound human BAF complex |
| 3J8A | 2014-12-10 | 3.7 | Structure of the F-actin-tropomyosin complex |
| 5OOD | 2018-06-13 | 3.7 | Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP-Pi |
| 6Q9Z | 2019-11-27 | 3.8 | Crystal structure of the pathological G167R variant of calcium-free human gelsolin, |
| 7BT7 | 2020-05-20 | 3.8 | F-actin-ADP complex structure |
| 5YU8 | 2018-05-23 | 3.8 | Cofilin decorated actin filament |
| 6ZNL | 2020-07-29 | 3.8 | Cryo-EM structure of the dynactin complex |
| 6C1G | 2018-01-31 | 3.8 | High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing |
| 6U96 | 2020-05-13 | 3.8 | Actin phalloidin at BeFx state |
| 6AV9 | 2018-01-17 | 3.9 | CryoEM structure of Mical Oxidized Actin (Class 1) |
| 6C1H | 2018-01-31 | 3.9 | High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing |
| 3CJC | 2008-03-25 | 3.9 | Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with DNase I and Gelsolin-segment 1 |
| 6AVB | 2018-01-17 | 3.9 | CryoEM structure of Mical Oxidized Actin (Class 1) |
| 6W17 | 2020-08-12 | 3.9 | Structure of Dip1-activated Arp2/3 complex with nucleated actin filament |
| 5JLH | 2016-06-15 | 3.9 | Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution |
| 5ADX | 2015-12-30 | 4.0 | CryoEM structure of dynactin complex at 4.0 angstrom resolution |
| 5ONV | 2018-06-13 | 4.1 | Cryo-EM structure of F-actin in complex with ADP |
| 6ZNM | 2020-07-29 | 4.1 | The pointed end complex of dynactin bound to BICDR1 |
| 7BTE | 2020-05-20 | 4.2 | Lifeact-F-actin complex |
| 6X5Z | 2020-07-22 | 4.24 | Bovine Cardiac Myosin in Complex with Chicken Skeletal Actin and Human Cardiac Tropomyosin in the Rigor State |
| 6ZNN | 2020-07-29 | 4.5 | The pointed end complex of dynactin bound to Hook3 |
| 6BNP | 2018-01-10 | 4.6 | CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state |
| 6BNV | 2018-01-10 | 4.6 | CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine |
| 3J8I | 2015-01-14 | 4.7 | Near-Atomic Resolution for One State of F-Actin |
| 6KN8 | 2020-01-15 | 4.8 | Structure of human cardiac thin filament in the calcium bound state |
| 6TF9 | 2019-12-11 | 4.8 | Structure of the vertebrate gamma-Tubulin Ring Complex |
| 5H53 | 2017-01-18 | 5.2 | The structure of rabbit skeletal muscle actomyosin rigor complex at 5.2 angstrom. |
| 6BNQ | 2018-01-10 | 5.5 | CryoEM structure of Myosin VI-Actin complex in the ADP state |
| 6BNO | 2018-01-10 | 5.5 | Structure of bare actin filament |
| 6BNW | 2018-01-10 | 5.5 | CryoEM structure of Myosin VI-Actin complex in the ADP state, backbone-averaged with side chains truncated to alanine |
| 3G37 | 2010-11-03 | 6.0 | Cryo-EM structure of actin filament in the presence of phosphate |
| 6BIH | 2018-09-19 | 6.0 | The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State |
| 6KN7 | 2020-01-15 | 6.6 | Structure of human cardiac thin filament in the calcium free state |
| 3MFP | 2010-09-29 | 6.6 | Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map |
| 6F38 | 2018-01-17 | 6.7 | Cryo-EM structure of two dynein tail domains bound to dynactin and HOOK3 |
| 6ZNO | 2020-07-29 | 6.8 | The pointed end complex of dynactin with the p150 projection docked |
| 6ANU | 2017-11-22 | 7.0 | Cryo-EM structure of F-actin complexed with the beta-III-spectrin actin-binding domain |
| 3M3N | 2010-07-28 | 7.0 | Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains |
| 6BNU | 2018-01-10 | 7.5 | Structure of bare actin filament, backbone-averaged with sidechains truncated to alanine |
| 6UBY | 2020-01-01 | 7.5 | Isolated cofilin bound to an actin filament |
| 6UC0 | 2020-01-01 | 7.5 | Isolated S3D-cofilin bound to an actin filament |
| 3JBJ | 2015-11-04 | 7.6 | Cryo-EM reconstruction of F-actin |
| 3J82 | 2015-05-20 | 7.7 | Electron cryo-microscopy of DNGR-1 in complex with F-actin |
| 4A7F | 2012-08-01 | 7.7 | Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 3) |
| 4A7H | 2012-08-01 | 7.8 | Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2) |
| 4V0U | 2015-03-25 | 7.88 | The crystal structure of ternary PP1G-PPP1R15B and G-actin complex |
| 1O1D | 2002-12-04 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 1O1G | 2002-12-11 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 1O1C | 2002-12-04 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 1O19 | 2002-11-27 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 1O1F | 2002-12-04 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 1O18 | 2002-11-27 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 1O1A | 2002-12-04 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 1O1E | 2002-12-04 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 1O1B | 2002-12-04 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 1M8Q | 2002-09-10 | 70.0 | Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle |
| 1MVW | 2002-11-20 | 70.0 | MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE |
| 3J4K | 2013-09-25 | 8.0 | Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state |
| 5NOL | 2017-07-19 | 8.0 | Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - 'Closed' state |
| 4A7L | 2012-08-01 | 8.1 | Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 1) |
| 3JBK | 2015-11-04 | 8.2 | Cryo-EM reconstruction of the metavinculin-actin interface |
| 6ZO4 | 2020-07-29 | 8.2 | The pointed end complex of dynactin bound to BICD2 |
| 6F3A | 2018-01-17 | 8.2 | Cryo-EM structure of a single dynein tail domain bound to dynactin and BICD2N |
| 5AFU | 2015-03-11 | 8.2 | Cryo-EM structure of dynein tail-dynactin-BICD2N complex |
| 3JBI | 2015-11-04 | 8.5 | MDFF model of the vinculin tail domain bound to F-actin |
| 4A7N | 2012-08-01 | 8.9 | Structure of bare F-actin filaments obtained from the same sample as the Actin-Tropomyosin-Myosin Complex |
| 3J0S | 2011-12-21 | 9.0 | Remodeling of actin filaments by ADF cofilin proteins |
| 6G2T | 2018-10-17 | 9.0 | human cardiac myosin binding protein C C1 Ig-domain bound to native cardiac thin filament |
| 5KG8 | 2016-09-07 | 9.1 | Rigor myosin X co-complexed with an actin filament |
| 6UC4 | 2020-01-01 | 9.2 | Barbed end side of a cofilactin cluster |
| 3B63 | 2008-11-18 | 9.5 | Actin filament model in the extended form of acromsomal bundle in the Limulus sperm |
Relevant UniProtKB Entries
| Percent Identity | Matching Chains | Protein | Accession | Entry Name |
|---|---|---|---|---|
| 97.6 | G | Major actin | Q68FP1 | GELS_RAT |
| 97.6 | G | Major actin | P13020 | GELS_MOUSE |
| 98.4 | G | Major actin | Q28372 | GELS_HORSE |
| 99.2 | G | Major actin | P20305 | GELS_PIG |
| 99.2 | G | Major actin | Q3SX14 | GELS_BOVIN |
| 100.0 | G | Major actin | P06396 | GELS_HUMAN |
| 96.9 | A | Major actin | Q24733 | ACT_DICVI |
| 97.8 | A | Major actin | P85911 | ACT1_PSEMZ |
| 97.6 | A | Major actin | Q39596 | ACT_OXYRB |
| 90.4 | A | Major actin | P10994 | ACTS_PLEWA |
| 95.5 | A | Major actin | P00544 | FGR_FSVGR |
| 92.6 | A | Major actin | P10982 | ACT1_ABSGL |
| 91.3 | A | Major actin | Q25379 | ACT3_LYTPI |
| 90.1 | A | Major actin | Q25381 | ACTM_LYTPI |
| 97.0 | A | Major actin | Q11212 | ACT_SPOLI |
| 96.9 | A | Major actin | P30170 | ACT10_SOLTU |
| 94.0 | A | Major actin | Q92193 | ACT_CRAVI |
| 91.7 | A | Major actin | Q92192 | ACT_CALFI |
| 92.6 | A | Major actin | Q03342 | ACT3_ECHGR |
| 90.5 | A | Major actin | P93585 | ACT4_SOLTU |
| 91.1 | A | Major actin | P93376 | ACT6_TOBAC |
| 91.1 | A | Major actin | P93587 | ACT1_SOLTU |
| 91.4 | A | Major actin | Q96483 | ACT2_SOLLC |
| 92.0 | A | Major actin | P93375 | ACT7_TOBAC |
| 91.4 | A | Major actin | P93371 | ACT5_TOBAC |
| 91.4 | A | Major actin | Q96484 | ACT3_SOLLC |
| 91.1 | A | Major actin | Q96482 | ACT1_SOLLC |
| 94.5 | A | Major actin | P18602 | ACT3_ARTSX |
| 92.0 | A | Major actin | P93372 | ACT4_TOBAC |
| 92.0 | A | Major actin | P93584 | ACT9_SOLTU |
| 92.0 | A | Major actin | P81229 | ACT8_SOLTU |
| 92.3 | A | Major actin | P81228 | ACT5_SOLTU |
| 92.2 | A | Major actin | P30172 | ACT12_SOLTU |
| 90.1 | A | Major actin | Q9Y701 | ACT1_SUIBO |
| 90.6 | A | Major actin | P12431 | ACTM_STRPU |
| 90.3 | A | Major actin | Q00214 | ACTM_STYPL |
| 90.4 | A | Major actin | P30164 | ACT1_PEA |
| 90.3 | A | Major actin | Q9Y702 | ACT1_SCHCO |
| 90.1 | A | Major actin | P53467 | ACTM_MOLOC |
| 97.2 | A | Major actin | Q55EU6 | ACT23_DICDI |
| 95.6 | A | Major actin | P84856 | ACTB_CHLPG |
| 90.6 | A | Major actin | P50138 | ACT_PUCGR |
| 91.2 | A | Major actin | Q9Y707 | ACT2_SUIBO |
| 90.1 | A | Major actin | P53504 | ACT1_SORBI |
| 91.4 | A | Major actin | P20904 | ACT_VOLCA |
| 90.7 | A | Major actin | P53498 | ACT_CHLRE |
| 91.4 | A | Major actin | P53689 | ACT_PHARH |
| 91.5 | A | Major actin | P26198 | ACTM_STYCL |
| 90.1 | A | Major actin | P53494 | ACT4_ARATH |
| 91.2 | A | Major actin | P53460 | ACT1_HALRO |
| 90.7 | A | Major actin | P53479 | ACTS_CYPCA |
| 90.7 | A | Major actin | P30173 | ACT13_SOLTU |
| 90.9 | A | Major actin | Q90X97 | ACTS_ATRMM |
| 90.6 | A | Major actin | P26197 | ACT2_ABSGL |
| 90.9 | A | Major actin | P68264 | ACTS_OREMO |
| 90.9 | A | Major actin | P68140 | ACTSA_TAKRU |
| 91.2 | A | Major actin | P53482 | ACTSB_TAKRU |
| 91.8 | A | Major actin | P53475 | ACTN_STYCL |
| 91.2 | A | Major actin | P10995 | ACT2_XENLA |
| 91.2 | A | Major actin | P68136 | ACTS_RAT |
| 91.2 | A | Major actin | P68135 | ACTS_RABIT |
| 91.2 | A | Major actin | Q5R9Q5 | ACTS_PONAB |
| 91.2 | A | Major actin | P68137 | ACTS_PIG |
| 91.2 | A | Major actin | Q98972 | ACTS_ORYLA |
| 91.2 | A | Major actin | P68134 | ACTS_MOUSE |
| 91.2 | A | Major actin | P68133 | ACTS_HUMAN |
| 91.2 | A | Major actin | P68139 | ACTS_CHICK |
| 91.2 | A | Major actin | P68138 | ACTS_BOVIN |
| 93.0 | A | Major actin | P53474 | ACTE_STRPU |
| 91.2 | A | Major actin | P49055 | ACTS_CARAU |
| 92.8 | A | Major actin | P18499 | ACTF_STRPU |
| 91.5 | A | Major actin | P53480 | ACTC_TAKRU |
| 91.2 | A | Major actin | P53492 | ACT7_ARATH |
| 90.4 | A | Major actin | P0CJ47 | ACT3_ARATH |
| 90.4 | A | Major actin | P0CJ46 | ACT1_ARATH |
| 92.2 | A | Major actin | O15998 | ACTM_CIOSA |
| 92.8 | A | Major actin | Q00215 | ACTC_STYPL |
| 91.7 | A | Major actin | P53459 | ACT6_DIPDE |
| 91.7 | A | Major actin | Q6P8G3 | ACT3_XENTR |
| 91.7 | A | Major actin | P04752 | ACT3_XENLA |
| 92.0 | A | Major actin | P27130 | ACT2_HALRO |
| 92.8 | A | Major actin | P53466 | ACT2_LYTPI |
| 90.7 | A | Major actin | P30171 | ACT11_SOLTU |
| 92.0 | A | Major actin | P04751 | ACTC_XENLA |
| 93.6 | A | Major actin | Q07903 | ACTC_STRPU |
| 91.2 | A | Major actin | Q10AZ4 | ACT3_ORYSJ |
| 91.2 | A | Major actin | A2XNS1 | ACT3_ORYSI |
| 91.7 | A | Major actin | P20399 | ACT2_XENTR |
| 90.9 | A | Major actin | O81221 | ACT_GOSHI |
| 92.2 | A | Major actin | P63269 | ACTH_RAT |
| 92.2 | A | Major actin | P63268 | ACTH_MOUSE |
| 92.2 | A | Major actin | P63267 | ACTH_HUMAN |
| 92.2 | A | Major actin | P63270 | ACTH_CHICK |
| 92.2 | A | Major actin | Q5E9B5 | ACTH_BOVIN |
| 92.0 | A | Major actin | Q6P640 | ACTC_XENTR |
| 92.0 | A | Major actin | P68035 | ACTC_RAT |
| 92.0 | A | Major actin | P68033 | ACTC_MOUSE |
| 92.0 | A | Major actin | P68032 | ACTC_HUMAN |
| 92.0 | A | Major actin | P68034 | ACTC_CHICK |
| 92.0 | A | Major actin | Q3ZC07 | ACTC_BOVIN |
| 92.2 | A | Major actin | P08023 | ACTA_CHICK |
| 92.5 | A | Major actin | P02574 | ACT4_DROME |
| 90.9 | A | Major actin | P30167 | ACT3_SOLTU |
| 92.2 | A | Major actin | Q25472 | ACT2_MOLOC |
| 90.9 | A | Major actin | Q05214 | ACT1_TOBAC |
| 92.5 | A | Major actin | P62738 | ACTA_RAT |
| 92.5 | A | Major actin | P62740 | ACTA_RABIT |
| 92.5 | A | Major actin | P62737 | ACTA_MOUSE |
| 92.5 | A | Major actin | P62736 | ACTA_HUMAN |
| 92.5 | A | Major actin | P62739 | ACTA_BOVIN |
| 92.8 | A | Major actin | P45887 | ACT5_BACDO |
| 93.6 | A | Major actin | P53465 | ACT1_LYTPI |
| 92.3 | A | Major actin | O65315 | ACT_COLSC |
| 94.4 | A | Major actin | P53473 | ACTB_STRPU |
| 91.5 | A | Major actin | Q10DV7 | ACT1_ORYSJ |
| 91.5 | A | Major actin | A2XLF2 | ACT1_ORYSI |
| 94.6 | A | Major actin | P10990 | ACT1_MESFR |
| 91.7 | A | Major actin | P53496 | ACT11_ARATH |
| 94.6 | A | Major actin | P69005 | ACTD_STRPU |
| 94.6 | A | Major actin | P69004 | ACT2_MESFR |
| 93.3 | A | Major actin | P91754 | ACT_LUMRU |
| 93.3 | A | Major actin | P12717 | ACTM_PISOC |
| 93.0 | A | Major actin | P53457 | ACT3_DIPDE |
| 93.3 | A | Major actin | P49128 | ACT1_AEDAE |
| 93.3 | A | Major actin | P07837 | ACT2_BOMMO |
| 93.3 | A | Major actin | P53463 | ACTM_HELER |
| 92.8 | A | Major actin | P18600 | ACT1_ARTSX |
| 94.1 | A | Major actin | P17304 | ACTM_APLCA |
| 94.6 | A | Major actin | P53472 | ACTA_STRPU |
| 93.8 | A | Major actin | P83968 | ACT6_DROSI |
| 93.8 | A | Major actin | P83967 | ACT6_DROME |
| 93.8 | A | Major actin | P83969 | ACT1_BACDO |
| 93.0 | A | Major actin | O16808 | ACT_MAYDE |
| 93.3 | A | Major actin | P53464 | ACTM_HELTB |
| 94.1 | A | Major actin | P92176 | ACT2_LUMTE |
| 93.3 | A | Major actin | P18601 | ACT2_ARTSX |
| 94.3 | A | Major actin | P53458 | ACT5_DIPDE |
| 94.9 | A | Major actin | P69003 | ACT1_HELTB |
| 94.9 | A | Major actin | P69002 | ACT1_HELER |
| 94.1 | A | Major actin | P41341 | ACTY_LIMPO |
| 93.3 | A | Major actin | P10981 | ACT5_DROME |
| 94.1 | A | Major actin | O18500 | ACT2_SACKO |
| 93.3 | A | Major actin | P53501 | ACT3_DROME |
| 93.6 | A | Major actin | P53456 | ACT2_DIPDE |
| 93.6 | A | Major actin | O17503 | ACTC_BRALA |
| 93.6 | A | Major actin | P92182 | ACT1_LUMTE |
| 93.6 | A | Major actin | P45885 | ACT2_BACDO |
| 94.1 | A | Major actin | P41113 | ACT3_PODCA |
| 93.6 | A | Major actin | P45886 | ACT3_BACDO |
| 93.8 | A | Major actin | P68555 | ACT_TAESO |
| 94.9 | A | Major actin | P41112 | ACT1_PODCA |
| 93.8 | A | Major actin | P68556 | ACT1_DIPDE |
| 93.8 | A | Major actin | P07836 | ACT1_BOMMO |
| 94.1 | A | Major actin | P49871 | ACT_MANSE |
| 94.6 | A | Major actin | P41339 | ACTA_LIMPO |
| 94.4 | A | Major actin | P17126 | ACT_HYDVU |
| 94.9 | A | Major actin | P84336 | ACTB_CAMDR |
| 94.9 | A | Major actin | P90689 | ACT_BRUMA |
| 94.4 | A | Major actin | Q964D9 | ACTC_PLATR |
| 94.4 | A | Major actin | Q964E0 | ACTC_BIOTE |
| 94.6 | A | Major actin | P92179 | ACTC_BIOGL |
| 94.6 | A | Major actin | Q0PGG4 | ACTB_BOSMU |
| 94.9 | A | Major actin | P53461 | ACTC_HALRO |
| 94.4 | A | Major actin | P53485 | ACTB2_TAKRU |
| 95.2 | A | Major actin | P63256 | ACTG_ANSAN |
| 94.6 | A | Major actin | Q964E3 | ACTC_BIOAL |
| 94.9 | A | Major actin | P53470 | ACT1_SCHMA |
| 94.4 | A | Major actin | O18499 | ACT1_SACKO |
| 94.9 | A | Major actin | Q964E2 | ACTC_BIOPF |
| 94.9 | A | Major actin | Q964E1 | ACTC_BIOOB |
| 94.6 | A | Major actin | P83750 | ACTB_CYPCA |
| 94.6 | A | Major actin | P83751 | ACTB_CTEID |
| 94.6 | A | Major actin | Q7ZVF9 | ACTB2_DANRE |
| 94.4 | A | Major actin | O17320 | ACT_CRAGI |
| 95.2 | A | Major actin | Q26065 | ACT_PLAMG |
| 95.2 | A | Major actin | P29751 | ACTB_RABIT |
| 94.9 | A | Major actin | Q7ZVI7 | ACTB1_DANRE |
| 94.9 | A | Major actin | O42161 | ACTB_SALSA |
| 95.4 | A | Major actin | P53506 | ACT8_XENLA |
| 94.4 | A | Major actin | P0DM42 | ACT3_CAEEL |
| 94.4 | A | Major actin | P0DM41 | ACT1_CAEEL |
| 95.4 | A | Major actin | P04829 | ACT3_BOMMO |
| 95.4 | A | Major actin | P15475 | ACTB_XENBO |
| 94.9 | A | Major actin | P79818 | ACTB_ORYLA |
| 94.6 | A | Major actin | P10986 | ACT4_CAEEL |
| 95.2 | A | Major actin | Q8JJB8 | ACTG_TRISC |
| 94.9 | A | Major actin | Q93131 | ACTC_BRAFL |
| 95.2 | A | Major actin | Q91ZK5 | ACTB_SIGHI |
| 95.2 | A | Major actin | P68143 | ACTB_OREMO |
| 95.2 | A | Major actin | P53486 | ACTB3_TAKRU |
| 95.2 | A | Major actin | P68142 | ACTB1_TAKRU |
| 95.2 | A | Major actin | P30163 | ACT2_ONCVO |
| 95.2 | A | Major actin | P48975 | ACTB_CRIGR |
| 95.2 | A | Major actin | P53478 | ACT5_CHICK |
| 95.2 | A | Major actin | P02572 | ACT2_DROME |
| 94.9 | A | Major actin | P30162 | ACT1_ONCVO |
| 96.0 | A | Major actin | P12716 | ACTC_PISOC |
| 95.4 | A | Major actin | P18603 | ACT4_ARTSX |
| 95.2 | A | Major actin | P41340 | ACT3_LIMPO |
| 95.4 | A | Major actin | Q6P378 | ACTG_XENTR |
| 95.2 | A | Major actin | Q93129 | ACTC_BRABE |
| 95.7 | A | Major actin | Q25010 | ACT3A_HELAM |
| 95.7 | A | Major actin | A2BDB0 | ACTG_XENLA |
| 95.7 | A | Major actin | P63257 | ACTG_TRIVU |
| 95.7 | A | Major actin | P63259 | ACTG_RAT |
| 95.7 | A | Major actin | Q5JAK2 | ACTG_PELLE |
| 95.7 | A | Major actin | P63260 | ACTG_MOUSE |
| 95.7 | A | Major actin | P63261 | ACTG_HUMAN |
| 95.7 | A | Major actin | Q5ZMQ2 | ACTG_CHICK |
| 95.7 | A | Major actin | P63258 | ACTG_BOVIN |
| 95.4 | A | Major actin | P60707 | ACTB_TRIVU |
| 95.4 | A | Major actin | Q4L0Y2 | ACTB_SPECI |
| 95.4 | A | Major actin | P60713 | ACTB_SHEEP |
| 95.4 | A | Major actin | P60711 | ACTB_RAT |
| 95.4 | A | Major actin | Q5R6G0 | ACTB_PONAB |
| 95.4 | A | Major actin | Q6QAQ1 | ACTB_PIG |
| 95.4 | A | Major actin | Q5R1X3 | ACTB_PANTR |
| 95.4 | A | Major actin | P60710 | ACTB_MOUSE |
| 95.4 | A | Major actin | Q711N9 | ACTB_MESAU |
| 95.4 | A | Major actin | Q4R561 | ACTB_MACFA |
| 95.4 | A | Major actin | P60709 | ACTB_HUMAN |
| 95.4 | A | Major actin | P60708 | ACTB_HORSE |
| 95.4 | A | Major actin | Q76N69 | ACTB_CHLAE |
| 95.4 | A | Major actin | P60706 | ACTB_CHICK |
| 95.4 | A | Major actin | Q71FK5 | ACTB_CAVPO |
| 95.4 | A | Major actin | O18840 | ACTB_CANLF |
| 95.4 | A | Major actin | P60712 | ACTB_BOVIN |
| 95.7 | A | Major actin | P53505 | ACT5_XENLA |
| 95.2 | A | Major actin | P53471 | ACT2_SCHMA |
| 95.2 | A | Major actin | P10984 | ACT2_CAEEL |
| 95.7 | A | Major actin | P84185 | ACT5C_ANOGA |
| 95.7 | A | Major actin | P84183 | ACT4_BOMMO |
| 95.7 | A | Major actin | P84184 | ACT3B_HELAM |
| 95.7 | A | Major actin | P10987 | ACT1_DROME |
| 95.7 | A | Major actin | Q6NVA9 | ACTB_XENTR |
| 95.7 | A | Major actin | O93400 | ACTB_XENLA |
| 96.5 | A | Major actin | P07829 | ACT3_DICDI |
| 97.6 | A | Major actin | P02578 | ACT1_ACACA |
| 98.4 | A | Major actin | Q553U6 | ACT22_DICDI |
| 98.9 | A | Major actin | P02576 | ACTA_PHYPO |
| 99.2 | A | Major actin | Q54GX7 | ACT10_DICDI |
| 99.5 | A | Major actin | P07830 | ACT1_DICDI |