Domain unfolding and the stability of thermolysin in guanidine hydrochloride.


Abstract

Equilibrium and kinetic studies of the unfolding and autolysis of the two domain protein thermolysin in guanidine hydrochloride are described. Enzyme activity, circular dichroism, fluorescence, sedimentation, size exclusion chromatography, and viscosity measurements were used to monitor conformational transitions and characterize the native and denatured states. The observation of biphasic transitions for the unfolding of apothermolysin and the spectroscopic changes associated with each phase of the overall unfolding process suggest unfolding of the N-terminal domain at less than 1 M guanidine hydrochloride, followed by the unfolding of the C-terminal domain, with the transition midpoint at 3 M guanidine hydrochloride. The refolding of the C-terminal domain is reversible; however, refolding of the N-terminal domain could not be demonstrated owing to protein aggregation. A quantitative analysis of the two transitions suggest that the unfolding of the two structural domains of thermolysin is not completely independent. Attempts to measure the unfolding of holothermolysin were hampered by autolysis. However, it was possible to show that at least three calcium ions serve to stabilize thermolysin against autolysis or unfolding in guanidine hydrochloride. Similar stabilization was observed for thermolysin with a single terbium ion bound at calcium site S(1). This result is consistent with our earlier findings, which suggest that calcium bound at sites S(1)-S(2) are located at a critical point on the unfolding pathway of thermolysin and serve to act as an interdomain lock. Study holds ProTherm entries: 10383 Extra Details: biphasic transitions; spectroscopic changes;,structural domains; unfolding pathway

Submission Details

ID: eed7rLxM4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:40 p.m.

Version: 1

Publication Details
Corbett RJ;Ahmad F;Roche RS,Biochem. Cell Biol. (1986) Domain unfolding and the stability of thermolysin in guanidine hydrochloride. PMID:3801183
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1TRL 1995-02-07 NMR SOLUTION STRUCTURE OF THE C-TERMINAL FRAGMENT 255-316 OF THERMOLYSIN: A DIMER FORMED BY SUBUNITS HAVING THE NATIVE STRUCTURE
5JVI 2016-12-21 1.12 Thermolysin in complex with JC148.
5N3V 2017-06-21 1.12 Thermolysin in complex with inhibitor JC292
4N4E 2014-04-02 1.13 Thermolysin in complex with UBTLN58
6GHX 2019-02-06 1.16 Alzheimer's Amyloid-Beta Peptide Fragment 31-35 in Complex with Cd-substituted Thermolysin
5JS3 2016-12-21 1.16 Thermolysin in complex with JC114.
4MZN 2014-04-02 1.17 Thermolysin in complex with UBTLN59
5ONQ 2018-08-29 1.17 Alzheimer's Amyloid-Beta Peptide Fragment 29-40 in Complex with Cd-substituted Thermolysin
5JSS 2016-12-21 1.19 Thermolysin in complex with JC149.
3DNZ 2009-07-07 1.2 Thermolysin by LB nanotemplate method before high X-Ray dose on ESRF ID14-2 beamline
5FSJ 2016-10-26 1.2 Structure of thermolysin prepared by the 'soak-and-freeze' method under 45 bar of oxygen pressure
5N2X 2017-06-21 1.21 Thermolysin in complex with inhibitor JC272
5M9W 2017-08-16 1.21 Experimental MAD phased structure of thermolysin in complex with inhibitor JC65.
5N34 2017-06-21 1.22 Thermolysin in complex with inhibitor JC276
3DO2 2009-07-07 1.22 Thermolysin by LB nanotemplate method after high X-Ray dose on ESRF ID14-2 beamline
4MWP 2014-04-02 1.23 Thermolysin in complex with UBTLN46
5LWD 2016-12-21 1.23 Thermolysin in complex with inhibitor (JC96)
5L3U 2016-12-21 1.23 Thermolysin in complex with JC149 (MPD cryo protectant)
5MNR 2017-06-21 1.25 Thermolysin in complex with inhibitor JC256
5L41 2016-12-21 1.25 Thermolysin in complex with JC148 (MPD cryo protectant)
5LVD 2017-08-16 1.25 Thermolysin in complex with inhibitor (JC67)
4N5P 2014-04-02 1.25 Thermolysin in complex with UBTLN20
5JT9 2016-12-21 1.26 Thermolysin in complex with JC106.
5A3Y 2015-11-11 1.27 SAD structure of Thermolysin obtained by multi crystal data collection
3T74 2012-08-01 1.28 Thermolysin In Complex With UBTLN27
3T87 2012-08-01 1.28 Thermolysin In Complex With UBTLN28
5L8P 2016-12-21 1.29 Thermolysin in complex with JC114 (PEG400 cryo protectant)
5MA7 2017-08-16 1.3 Structure of thermolysin in complex with inhibitor (JC306).
4OI5 2014-04-02 1.3 Glycerol-free structure of thermolysin in complex with ubtln58
5LIF 2016-12-21 1.31 Thermolysin in complex with inhibitor
4MTW 2014-04-02 1.32 Thermolysin in complex with UBTLN36
3FGD 2009-12-15 1.33 Drugscore FP: Thermoylsin in complex with fragment.
5M5F 2017-08-16 1.33 Thermolysin in complex with inhibitor and krypton
3DO1 2009-07-07 1.33 Thermolysin by Classical hanging drop method before high X-Ray dose on ESRF ID14-2 beamline
5N3Y 2017-06-21 1.34 Thermolysin in complex with inhibitor JC267
5ONP 2018-08-29 1.34 Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Cd-substituted Thermolysin
5DPE 2015-10-28 1.34 Thermolysin in complex with inhibitor.
4MXJ 2014-04-02 1.35 Thermolysin in complex with UBTLN35
3DO0 2009-07-07 1.36 Thermolysin by classical hanging drop method after high X-Ray dose on esrf ID14-2 beamline
5N2Z 2017-06-21 1.37 Thermolysin in complex with inhibitor JC286
5N31 2017-06-21 1.37 Thermolysin in complex with inhibitor JC277
5JXN 2016-12-21 1.38 Thermolysin in complex with JC240.
5N2T 2017-06-21 1.38 Thermolysin in complex with inhibitor JC287
4D9W 2013-01-16 1.38 Thermolysin In Complex With UBTLN32
5ONR 2018-08-29 1.39 Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Thermolysin
6FSM 2018-05-02 1.39 Crystal structure of TCE-treated Thermolysin
3FVP 2010-02-09 1.41 Thermolysin inhibition
3T8D 2012-08-01 1.41 Thermolysin In Complex With UBTLN31
6FJ2 2018-01-31 1.43 Structure of Thermolysin solved from SAD data collected at the peak of the Zn absorption edge on ID30B
5M69 2017-08-16 1.44 Thermolysin in complex with inhibitor and xenon
4N66 2014-04-02 1.44 Thermolysin in complex with UBTLN37
3T8F 2012-08-01 1.44 Thermolysin In Complex With UBTLN34
3T8H 2012-08-01 1.45 Thermolysin In Complex With UBTLN29
5FXN 2016-03-16 1.45 Structure of thermolysin solved by SAD from data collected by Direct Data Collection (DDC) using the ESRF RoboDiff goniometer
3QGO 2012-01-04 1.45 Structure of Thermolysin in complex with L-Phenylalanine methylester
5DPF 2015-10-28 1.47 Thermolysin in complex with inhibitor.
5FSS 2016-10-26 1.5 Structure of thermolysin prepared by the 'soak-and-freeze' method under 40 bar of krypton pressure
3QH5 2012-01-04 1.5 Structure of Thermolysin in complex with N-Carbobenzyloxy-L-aspartic acid and L-Phenylalanine Methyl Ester
3T8G 2012-08-01 1.5 Thermolysin In Complex With UBTLN26
6QF3 2019-07-10 1.52 X-Ray structure of Thermolysin soaked with sodium aspartate on a silicon chip
3MS3 2011-05-11 1.54 Crystal structure of Thermolysin in complex with Aniline
3QH1 2012-01-04 1.55 Structure of Thermolysin in complex with N-benzyloxycarbonyl-L-aspartic acid
3FXS 2010-02-09 1.55 Metal exchange in thermolysin
4H57 2012-10-03 1.56 Thermolysin inhibition
3FV4 2010-02-09 1.56 Thermolysin inhibition
2TMN 1989-01-09 1.6 CRYSTALLOGRAPHIC STRUCTURAL ANALYSIS OF PHOSPHORAMIDATES AS INHIBITORS AND TRANSITION-STATE ANALOGS OF THERMOLYSIN
5UUE 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant
1KEI 2002-11-16 1.6 Thermolysin (substrate-free)
3FB0 2009-12-08 1.6 Metal exchange in thermolysin
5UN3 2017-02-22 1.6 Tetragonal thermolysin (295 K) in the presence of 50% xylose
1KKK 2002-12-10 1.6 Thermolysin complexed with Z-L-Aspartic Acid (benzyloxycarbonyl-L-Aspartic Acid)
5UUA 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant
5UU7 2017-03-08 1.6 Tetragonal thermolysin (295 K) in the presence of 50% mpd
1KJO 2002-12-05 1.6 Thermolysin complexed with Z-L-Threonine (benzyloxycarbonyl-L-Threonine)
5UU9 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 40% xylose as cryoprotectant
5UUC 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% mpd as cryoprotectant
3T73 2012-08-01 1.6 Thermolysin In Complex With UBTLN22
5TMN 1989-01-09 1.6 Slow-and fast-binding inhibitors of thermolysin display different modes of binding. crystallographic analysis of extended phosphonamidate transition-state analogues
6TMN 1989-01-09 1.6 Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond
8TLN 1994-04-30 1.6 STRUCTURAL COMPARISON SUGGESTS THAT THERMOLYSIN AND RELATED NEUTRAL PROTEASES UNDERGO HINGE-BENDING MOTION DURING CATALYSIS
5UUD 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% dmf as cryoprotectant
5UUB 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 25% xylose/25% mpd as cryoprotectant
1KJP 2002-12-05 1.6 Thermolysin complexed with Z-L-Glutamic acid (benzyloxycarbonyl-L-Glutamic acid)
2TLX 2000-03-13 1.65 THERMOLYSIN (NATIVE)
3MSA 2011-05-11 1.66 Crystal structure of Thermolysin in complex with 3-Bromophenol
3T8C 2012-08-01 1.66 Thermolysin In Complex With UBTLN30
3F28 2009-11-17 1.68 Thermolysin inhibition
1ZDP 2005-04-26 1.7 Crystal Structure Analysis of Thermolysin Complexed with the Inhibitor (S)-thiorphan
1KS7 2003-01-11 1.7 Thermolysin complexed with Z-D-Aspartic acid (benzyloxycarbonyl-D-Aspartic acid)
6FHP 2018-09-12 1.7 DAIP in complex with a C-terminal fragment of thermolysin
1KRO 2003-01-10 1.7 Thermolysin complexed with Z-D-Threonine (benzyloxycarbonyl-D-Threonine)
1FJQ 2001-04-18 1.7 THERMOLYSIN (70% ACETONE SOAKED CRYSTALS)
1Z9G 2005-04-19 1.7 Crystal Structure Analysis of Thermolysin Complexed with the Inhibitor (R)-retro-thiorphan
1HYT 1994-07-31 1.7 RE-DETERMINATION AND REFINEMENT OF THE COMPLEX OF BENZYLSUCCINIC ACID WITH THERMOLYSIN AND ITS RELATION TO THE COMPLEX WITH CARBOXYPEPTIDASE A
4TMN 1989-01-09 1.7 SLOW-AND FAST-BINDING INHIBITORS OF THERMOLYSIN DISPLAY DIFFERENT MODES OF BINDING. CRYSTALLOGRAPHIC ANALYSIS OF EXTENDED PHOSPHONAMIDATE TRANSITION-STATE ANALOGUES
1PE5 2004-06-08 1.7 Thermolysin with tricyclic inhibitor
1LND 1995-05-08 1.7 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
1LNF 1995-05-08 1.7 A structural analysis of metal substitutions in thermolysin
1THL 1994-01-31 1.7 Thermolysin complexed with a novel glutaramide derivative, n-(1-(2(r,s)-carboxy-4-phenylbutyl) cyclopentylcarbonyl)-(s)-tryptophan
5FSP 2016-10-26 1.7 Structure of thermolysin prepared by the 'soak-and-freeze' method under 100 bar of krypton pressure
3TMN 1989-01-09 1.7 THE BINDING OF L-VALYL-L-TRYPTOPHAN TO CRYSTALLINE THERMOLYSIN ILLUSTRATES THE MODE OF INTERACTION OF A PRODUCT OF PEPTIDE HYDROLYSIS
1LNE 1995-05-08 1.7 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
6QF2 2019-07-10 1.73 X-Ray structure of Thermolysin crystallized on a silicon chip
2WHZ 2010-05-12 1.75 Dipeptide Inhibitors of Thermolysin
3FCQ 2009-12-08 1.75 Thermolysin inhibition
3SSB 2011-09-07 1.8 Structure of Insect Metalloproteinase Inhibitor in Complex with Thermolysin
1PE8 2004-06-08 1.8 Thermolysin with monocyclic inhibitor
4TNL 2014-07-09 1.8 1.8 A resolution room temperature structure of Thermolysin recorded using an XFEL
1KL6 2002-12-11 1.8 Thermolysin complexed with Z-L-Alanine (benzyloxycarbonyl-L-Alanine)
1KR6 2003-01-09 1.8 Thermolysin complexed with Z-D-Glutamic acid (benzyloxycarbonyl-D-Glutamic acid)
1LNB 1995-05-08 1.8 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
6IG7 2018-11-07 1.8 Crystal structure of thermolysin delivered in polyacrylamide using x-ray free electron laser
1LNC 1995-05-08 1.8 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
1PE7 2004-06-08 1.82 Thermolysin with bicyclic inhibitor
2A7G 2005-07-19 1.85 On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength
3N21 2011-05-25 1.87 Crystal structure of Thermolysin in complex with S-1,2-Propandiol
3EIM 2009-10-06 1.88 Metal exchange in Thermolysin
1FJW 2001-04-18 1.9 THERMOLYSIN (50 MM PHENOL SOAKED)
5WR5 2017-08-16 1.9 Thermolysin, liganded form with cryo condition 1
1LNA 1995-05-08 1.9 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
1KTO 2003-01-17 1.9 Thermolysin complexed with Z-D-Alanine (benzyloxycarbonyl-D-Alanine)
5O8N 2017-08-09 1.9 Structure of thermolysin at room temperature via a method of acoustically induced rotation.
1TMN 1989-01-09 1.9 Binding of n-carboxymethyl dipeptide inhibitors to thermolysin determined by x-ray crystallography. a novel class of transition-state analogues for zinc peptidases
4D91 2013-01-16 1.9 Thermolysin In Complex With DMSO And Acetate
3FBO 2009-12-08 1.92 Metal exchange in Thermolysin
3FOR 2010-01-12 1.93 Thermolysin Complexed with BNPA (2-Benzyl-3-Nitro Propanoic Acid Amide)
3T2H 2011-12-28 1.95 Tetragonal thermolysin in the presence of TMAO
3TLI 2000-02-18 1.95 THERMOLYSIN (10% ISOPROPANOL SOAKED CRYSTALS)
7TLI 2000-03-13 1.95 THERMOLYSIN (90% ISOPROPANOL SOAKED CRYSTALS)
2TLI 2000-02-18 1.95 THERMOLYSIN (5% ISOPROPANOL SOAKED CRYSTALS)
3F2P 2009-11-17 1.95 Thermolysin inhibition
2WI0 2010-05-12 1.95 Dipeptide Inhibitors of Thermolysin
4TLI 2000-03-13 1.95 THERMOLYSIN (25% ISOPROPANOL SOAKED CRYSTALS)
3FLF 2010-01-12 1.97 Thermolysin inhibition
3MSN 2011-05-11 1.97 Crystal structure of Thermolysin in complex with N-methylurea
2G4Z 2007-02-20 1.98 anomalous substructure of thermolysin
3LS7 2011-02-23 1.98 Crystal structure of Thermolysin in complex with Xenon
1FJV 2001-04-18 2.0 THERMOLYSIN (60% ACETONITRILE SOAKED CRYSTALS)
6D5U 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant
1FJO 2001-04-18 2.0 THERMOLYSIN (60% ACETONE SOAKED CRYSTALS)
6D5N 2018-10-31 2.0 Hexagonal thermolysin (295) in the presence of 50% xylose
6D5T 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant
6D5Q 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant
1FJ3 2001-04-18 2.0 THERMOLYSIN (50% ACETONE SOAKED)
1FJU 2001-04-18 2.0 THERMOLYSIN (80% ACETONITRILE SOAKED CRYSTALS)
3ZI6 2013-05-01 2.0 Structure of thermolysin solved by SAD from data collected by Direct Data Collection (DDC) using the GRob robot goniometer
3T2J 2011-12-28 2.0 Tetragonal thermolysin in the presence of betaine
6D5O 2018-09-19 2.0 Hexagonal thermolysin (295 K) in the presence of 50% DMF
5TAK 2017-08-30 2.0 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5WR2 2017-08-16 2.0 Thermolysin, SFX liganded form with oil-based carrier
6D5S 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant
6D5R 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant
1QF1 1999-12-29 2.0 THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYLHEPTANOYL)-PHE-ALA. PARAMETERS FOR ZN-BIDENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE
5TAJ 2017-08-30 2.03 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5TAC 2017-08-30 2.04 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
3NN7 2011-05-25 2.05 Crystal structure of Thermolysin in complex with 2-bromoacetate
3FXP 2010-02-09 2.05 Thermolysin inhibition
1TLI 2000-02-18 2.05 THERMOLYSIN (2% ISOPROPANOL SOAKED CRYSTALS)
1QF2 1999-12-29 2.06 THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYL-3-PHENYLPROPANOYL)-GLY-(5-PHENYLPROLINE). PARAMETERS FOR ZN-MONODENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE
5T9I 2017-08-30 2.09 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5TAD 2017-08-30 2.09 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
3MSF 2011-05-11 2.09 Crystal structure of Thermolysin in complex with Urea
5T9K 2017-08-30 2.1 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5T9Q 2017-08-30 2.1 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
1Y3G 2006-01-17 2.1 Crystal Structure of a Silanediol Protease Inhibitor Bound to Thermolysin
5TLI 2000-03-13 2.1 THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)
6TLI 2000-03-13 2.1 THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)
5WR3 2017-08-16 2.1 Thermolysin, SFX liganded form with water-based carrier
3T2I 2011-12-28 2.1 Tetragonal thermolysin in the presence of sarcosine
1OS0 2003-03-25 2.1 Thermolysin with an alpha-amino phosphinic inhibitor
4OW3 2014-03-12 2.1 Thermolysin structure determined by free-electron laser
1TLX 2000-03-13 2.1 THERMOLYSIN (NATIVE)
5WR4 2017-08-16 2.1 Thermolysin, SFX unliganded form with oil-based carrier
1GXW 2002-12-05 2.18 the 2.2 A resolution structure of thermolysin crystallized in presence of potassium thiocyanate
1QF0 1999-12-29 2.2 THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYL-3-PHENYLPROPANOYL)-PHE-TYR. PARAMETERS FOR ZN-BIDENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE
3P7Q 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure B (2.5 MGy)
3P7U 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure B (2.4 MGy)
3P7V 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure C (4.8 MGy)
3P7T 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure A (0.1 MGy)
1FJT 2001-04-18 2.2 THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS)
3P7P 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure A (0.1 MGy)
3P7W 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure D (7.1 MGy)
3P7S 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure D (7.2 MGy)
8TLI 2000-03-13 2.2 THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)
3P7R 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure C (4.9 MGy)
5DLH 2015-11-18 2.25 SFX structure of thermolysin
5WR6 2017-08-16 2.3 Thermolysin, liganded form with cryo condition 2
1L3F 2002-07-03 2.3 Thermolysin in the Absence of Substrate has an Open Conformation
5TLN 1982-05-26 2.3 BINDING OF HYDROXAMIC ACID INHIBITORS TO CRYSTALLINE THERMOLYSIN SUGGESTS A PENTACOORDINATE ZINC INTERMEDIATE IN CATALYSIS
5TAI 2017-08-30 2.3 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
1TLP 1989-01-09 2.3 CRYSTALLOGRAPHIC STRUCTURAL ANALYSIS OF PHOSPHORAMIDATES AS INHIBITORS AND TRANSITION-STATE ANALOGS OF THERMOLYSIN
7TLN 1983-03-09 2.3 STRUCTURAL ANALYSIS OF THE INHIBITION OF THERMOLYSIN BY AN ACTIVE-SITE-DIRECTED IRREVERSIBLE INHIBITOR
5TAE 2017-08-30 2.3 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
4TLN 1982-05-26 2.3 BINDING OF HYDROXAMIC ACID INHIBITORS TO CRYSTALLINE THERMOLYSIN SUGGESTS A PENTACOORDINATE ZINC INTERMEDIATE IN CATALYSIS
5UU8 2017-03-08 2.5 Tetragonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant
5K7T 2017-04-05 2.5 MicroED structure of thermolysin at 2.5 A resolution
6D5P 2018-09-19 3.0 Hexagonal thermolysin cryocooled to 100 K with 20% xylose as cryoprotectant

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.4 Thermolysin P43133 THER_GEOSE
100.0 Thermolysin P00800 THER_BACTH