The conformational stability of alpha-crystallin is rather low: calorimetric results.


Abstract

The eye lens protein and chaperonin, alpha-crystallin, was studied by differential scanning microcalorimetry, spectroscopy and size exclusion chromatography. The thermal transition of alpha-crystallin proceeds at Ttrs = 59.8 +/- 0.6 degrees C with an enthalpy change of delta H = 336 +/- 9 kJ per mol subunit. Disagreement between previous delta H values could be attributed to a side reaction that leads, depending on the scan rate, to the formation of a non-productive folding form. The conformational stability of alpha-crystallin is rather low (delta G = 24 +/- 5 kJ/mol of subunit). The minimal cooperative unit of alpha-crystallin is the monomeric subunit. Study holds ProTherm entries: 10810, 10811 Extra Details: alpha-crystallin; heat shock protein; scanning calorimetry;,conformational stability; non-productive folding form

Submission Details

ID: e5VFfrDe

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:41 p.m.

Version: 1

Publication Details
Gesierich U;Pfeil W,FEBS Lett. (1996) The conformational stability of alpha-crystallin is rather low: calorimetric results. PMID:8814280
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3L1E 2010-05-12 1.15 Bovine AlphaA crystallin Zinc Bound
3L1F 2010-05-12 1.53 Bovine AlphaA crystallin

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
90.8 Alpha-crystallin A chain P02487 CRYAA_BRAVA
90.2 Alpha-crystallin A chain P02502 CRYAA_MACRU
91.9 Alpha-crystallin A chain P02486 CRYAA_CHOHO
90.8 Alpha-crystallin A chain P02501 CRYAA_ORYAF
92.5 Alpha-crystallin A chain P82533 CRYAA_ERIEU
92.5 Alpha-crystallin A chain P02499 CRYAA_PROCA
94.8 Alpha-crystallin A chain P02488 CRYAA_MACMU
94.2 Alpha-crystallin A chain P02498 CRYAA_LOXAF
94.2 Alpha-crystallin A chain P02489 CRYAA_HUMAN
94.2 Alpha-crystallin A chain A0A140G945 CRYA2_HUMAN
95.4 Alpha-crystallin A chain P02484 CRYAA_MANJA
96.0 Alpha-crystallin A chain P68406 CRYAA_TUPGL
96.0 Alpha-crystallin A chain P68405 CRYAA_MERUN
95.4 Alpha-crystallin A chain P68286 CRYAA_PERPO
95.4 Alpha-crystallin A chain P68287 CRYAA_OTOCR
96.0 Alpha-crystallin A chain P02492 CRYAA_OCHPR
96.0 Alpha-crystallin A chain P02483 CRYAA_NEOVI
96.5 Alpha-crystallin A chain P68288 CRYAA_ZALCA
96.5 Alpha-crystallin A chain P68289 CRYAA_HALGR
96.0 Alpha-crystallin A chain P02494 CRYAA_EULFU
97.1 Alpha-crystallin A chain P02482 CRYAA_ARTJA
96.5 Alpha-crystallin A chain P68283 CRYAA_PEDCA
96.5 Alpha-crystallin A chain P68281 CRYAA_CAVPO
97.1 Alpha-crystallin A chain P02477 CRYAA_PHOPH
97.1 Alpha-crystallin A chain P02480 CRYAA_MELUS
97.1 Alpha-crystallin A chain P02478 CRYAA_HORSE
97.1 Alpha-crystallin A chain P02493 CRYAA_RABIT
98.3 Alpha-crystallin A chain P68282 CRYAA_FELCA
98.3 Alpha-crystallin A chain P68280 CRYAA_CANLF
98.8 Alpha-crystallin A chain P02472 CRYAA_CAMDR
97.7 Alpha-crystallin A chain P02479 CRYAA_CERSI
97.7 Alpha-crystallin A chain P02475 CRYAA_PIG
98.3 Alpha-crystallin A chain P02474 CRYAA_BALAC
98.3 Alpha-crystallin A chain P02476 CRYAA_TAPIN
98.8 Alpha-crystallin A chain P82531 CRYAA_PTEPO
99.4 Alpha-crystallin A chain P68285 CRYAA_HIPAM
99.4 Alpha-crystallin A chain P68284 CRYAA_GIRCA
99.4 Alpha-crystallin A chain Q5ENZ0 CRYAA_SHEEP
100.0 Alpha-crystallin A chain P02470 CRYAA_BOVIN