Deletion of the omega-loop in the active site of staphylococcal nuclease. 1. Effect on catalysis and stability.
Abstract
The high-resolution X-ray structure of wild-type staphylococcal nuclease (E43 SNase) suggests that Glu 43 acts a general basic catalyst to assist the attack of water on a phosphodiester substrate [Loll, P., & Lattman, E. E. (1989) Proteins: Struct., Funct., Genet. 5, 183]. Glu 43 is located at the base of the solvent-exposed and conformationally mobile omega-loop in the active site of E43 SNase having the sequence Glu43-Thr44-Lys45-His46-Pro47-Lys48- Lys49-Gly50-Val51-Glu52, where the gamma-carboxylate of Glu 52 is hydrogen bonded to the amide hydrogen of Glu 43. With a metabolic selection for SNase activity produced in an Escherichia coli host, we detected an unexpected deletion of residues 44-49 of the omega-loop of E43 SNase in cassette mutagenesis experiments designed to randomize codons 44 and 45 in the omega-loop and increase the activity of the previously described E43D mutation (D43 SNase). A high-resolution X-ray structure of D43 SNase has revealed that the E43D substitution significantly changes the structure of the omega-loop, reduces the interaction of the essential Ca2+ ion with its active-site ligands, and diminishes the network of hydrogen-bonded water molecules in the active site [Loll, P., & Lattman, E. E. (1990) Biochemistry 29, 6866]. This deletion of six amino acids from the omega-loop generates a protein (E43 delta SNase) having a partially solvent-exposed, surface beta-turn with the sequence Glu43-Gly50-Val51-Glu52; the structure of this beta-turn is addressed in the following article [Baldisseri et al. (1991) Biochemistry (following paper in this issue)].(ABSTRACT TRUNCATED AT 250 WORDS) Study holds ProTherm entries: 4243, 4244 Extra Details:
Submission Details
ID: c6jivvr74
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:25 p.m.
Version: 1
Publication Details
Poole LB;Loveys DA;Hale SP;Gerlt JA;Stanczyk SM;Bolton PH,Biochemistry (1991) Deletion of the omega-loop in the active site of staphylococcal nuclease. 1. Effect on catalysis and stability. PMID:2015219Additional Information
Study Summary
| Number of data points | 7 |
| Proteins | Thermonuclease ; Thermonuclease |
| Unique complexes | 2 |
| Assays/Quantities/Protocols | Experimental Assay: Cm ; Experimental Assay: m ; Experimental Assay: dG_H2O ; Derived Quantity: ddG_H2O |
| Libraries | Mutations for sequence ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ |
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
|---|
Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant PDB Entries
| Structure ID | Release Date | Resolution | Structure Title |
|---|---|---|---|
| 2SOB | 1995-12-07 | SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES | |
| 1JOO | 2001-08-22 | Averaged structure for unligated Staphylococcal nuclease-H124L | |
| 1JOK | 2001-08-22 | Averaged structure for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate | |
| 2KHS | 2009-10-20 | Solution structure of SNase121:SNase(111-143) complex | |
| 2M00 | 2013-03-06 | Solution structure of staphylococcal nuclease E43S mutant in the presence of ssDNA and Cd2+ | |
| 2PQE | 2007-06-19 | Solution structure of proline-free mutant of staphylococcal nuclease | |
| 1JOQ | 2001-08-22 | Ensemble structures for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate | |
| 1JOR | 2001-08-22 | Ensemble structures for unligated Staphylococcal nuclease-H124L | |
| 2LKV | 2012-09-12 | Staphylococcal Nuclease PHS variant | |
| 2FXZ | 2006-12-19 | Solution structure of 97-109 segment of staphylococcal nuclease | |
| 4EQP | 2012-05-02 | 1.35 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D at cryogenic temperature |
| 4HMJ | 2012-10-31 | 1.35 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D at cryogenic temperature |
| 4DFA | 2012-02-01 | 1.4 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L36A at cryogenic temperature |
| 3TME | 2011-09-14 | 1.4 | Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E at cryogenic temperature |
| 3SXH | 2011-07-27 | 1.4 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92AL103A at cryogenic temperature |
| 4KY5 | 2013-06-12 | 1.4 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D at pH 7 and cryogenic temperature |
| 4N9P | 2013-12-04 | 1.4 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L25A/V99T at cryogenic temperature |
| 3SR1 | 2011-07-27 | 1.45 | Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature |
| 5JOB | 2016-05-11 | 1.45 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92A at cryogenic temperature |
| 4DGZ | 2012-02-08 | 1.47 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L125A at cryogenic temperature |
| 3SHL | 2011-06-29 | 1.48 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V74KL25A at cryogenic temperature |
| 2SNS | 1982-07-29 | 1.5 | STAPHYLOCOCCAL NUCLEASE. PROPOSED MECHANISM OF ACTION BASED ON STRUCTURE OF ENZYME-THYMIDINE 3(PRIME),5(PRIME)-BIPHOSPHATE-CALCIUM ION COMPLEX AT 1.5-ANGSTROMS RESOLUTION |
| 3MEH | 2011-03-16 | 1.5 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A at cryogenic temperature |
| 4NP5 | 2013-12-04 | 1.5 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92N at cryogenic temperature |
| 5HUR | 2016-02-10 | 1.5 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25T/I92K at cryogenic temperature |
| 4DF7 | 2012-02-01 | 1.5 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23L/V99I at cryogenic temperature |
| 5DAU | 2015-09-02 | 1.5 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V39E/V104E at cryogenic temperature |
| 5KEE | 2016-06-22 | 1.5 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92F at cryogenic temperature |
| 4NDX | 2013-11-13 | 1.5 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N at cryogenic temperature |
| 3LX0 | 2011-02-16 | 1.5 | Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N at cryogenic temperature |
| 4KD4 | 2013-05-08 | 1.55 | Crystal structure of Staphylococcal nuclease variant V23L/L25V/V66I/I72V at cryogenic temperature |
| 4K2L | 2013-04-24 | 1.55 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92Q at cryogenic temperature |
| 3VA5 | 2012-01-25 | 1.55 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V23A at cryogenic temperature |
| 4KJN | 2013-05-15 | 1.55 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66A/V99T at cryogenic temperature |
| 4KY7 | 2013-06-12 | 1.55 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V74D at cryogenic temperature |
| 3MHB | 2011-03-23 | 1.55 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L38A at cryogenic temperature |
| 4KV6 | 2013-06-12 | 1.55 | Crystal structure of Staphylococcal nuclease variant Delta+PHS R126Q at cryogenic temperature |
| 3RUZ | 2011-06-15 | 1.58 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V74K at cryogenic temperature |
| 4LAA | 2013-08-21 | 1.58 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36H at cryogenic temperature |
| 3MZ5 | 2011-03-23 | 1.58 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L103A at cryogenic temperature |
| 5CV8 | 2015-08-12 | 1.58 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/T62H at cryogenic temperature |
| 4F8M | 2012-06-13 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23I/I92V at cryogenic temperature |
| 4TRD | 2014-07-16 | 1.6 | Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K/V74K at pH 9.0 at cryogenic temperature |
| 4J6H | 2013-03-06 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS H121Q at cryogenic temperature |
| 4K14 | 2013-04-17 | 1.6 | Crystal Structure of Staphylococcal nuclease mutant V66I/V99L |
| 1EY0 | 2000-10-18 | 1.6 | STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.6 A RESOLUTION |
| 4H7B | 2012-10-17 | 1.6 | Crystal Structure of Staphylococcal nuclease mutant I72V/V99L |
| 3TP8 | 2011-09-21 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E at cryogenic temperature |
| 4IAL | 2013-01-09 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS H121E at cryogenic temperature |
| 4WOR | 2014-10-29 | 1.6 | Staphylococcal nuclease in complex with Ca2+ and thymidine-3'-5'-diphosphate (pdTp) at room temperature |
| 1EY4 | 2000-10-18 | 1.6 | STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S59A |
| 5KRU | 2016-07-27 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66K at cryogenic temperature |
| 3QOJ | 2011-03-16 | 1.6 | Cryogenic structure of Staphylococcal nuclease variant D+PHS/V23K |
| 4QB4 | 2014-06-11 | 1.6 | Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L |
| 3OSO | 2011-09-21 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A at cryogenic temperature |
| 4KHV | 2013-05-15 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S at cryogenic temperature |
| 4IUN | 2013-02-06 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS R126E at cryogenic temperature |
| 4NKL | 2013-11-20 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92Q at cryogenic temperature |
| 3PMF | 2011-11-16 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23A at cryogenic temperature |
| 5IOD | 2016-03-23 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99E at cryogenic temperature |
| 4N9T | 2013-10-30 | 1.6 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92S at cryogenic temperature |
| 3MXP | 2011-03-23 | 1.61 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature |
| 4I65 | 2013-03-20 | 1.61 | Crystal Structure of Staphylococcal nuclease mutant V23I/L25V/I72V/I92V |
| 5CV9 | 2015-08-12 | 1.62 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/V66H at cryogenic temperature |
| 5C4Z | 2015-07-15 | 1.62 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25H at cryogenic temperature |
| 4DU9 | 2012-03-07 | 1.63 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V74A at cryogenic temperature |
| 3QB3 | 2012-01-18 | 1.63 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92KL25A at cryogenic temperature |
| 4S3S | 2015-07-01 | 1.64 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K/V23A at cryogenic temperature |
| 4YIJ | 2015-03-18 | 1.64 | Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature |
| 4R8N | 2014-09-17 | 1.65 | Crystal structure of Staphylococcal nuclease variant V23I/V66I/I72V/I92V at cryogenic temperature |
| 3NK9 | 2011-06-29 | 1.65 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V74A at cryogenic temperature |
| 4PMC | 2014-06-11 | 1.65 | Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K V74K at cryogenic temperature |
| 4K2K | 2013-04-24 | 1.65 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/L36A/I92A at cryogenic temperature |
| 1IHZ | 2003-06-17 | 1.65 | Structure of S. nuclease mutant quintuple mutant V23L/V66L/I72L/I92L/V99L |
| 4J1M | 2013-02-20 | 1.65 | Crystal structure of Staphylococcal nuclease variant Delta+PHS R105E at cryogenic temperature |
| 5CV7 | 2015-08-12 | 1.65 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92D at cryogenic temperature |
| 4K5W | 2013-04-24 | 1.65 | Crystal structure of Staphylococcal nuclease variant V23M/L25F/T62F at cryogenic temperature |
| 2EY6 | 2006-10-24 | 1.65 | Crystal structure of Staphylococcal nuclease mutant T41V |
| 3NXW | 2011-07-20 | 1.65 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L125A at cryogenic temperature |
| 4WRD | 2015-07-08 | 1.65 | Crystal structure of Staphylcoccal nulease variant Delta+PHS V66E L125E at cryogenic temperature |
| 4K6D | 2013-04-24 | 1.65 | Crystal structure of Staphylococcal nuclease variant V23M/T62F at cryogenic temperature |
| 4K5X | 2013-04-24 | 1.65 | Crystal structure of Staphylococcal nuclease variant V23M/L36F at cryogenic temperature |
| 6B8R | 2017-10-18 | 1.65 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36Q at cryogenic temperature |
| 1SNC | 1990-10-15 | 1.65 | THE CRYSTAL STRUCTURE OF THE TERNARY COMPLEX OF STAPHYLOCOCCAL NUCLEASE, CA2+, AND THE INHIBITOR PD*TP, REFINED AT 1.65 ANGSTROMS |
| 4RKL | 2014-11-26 | 1.66 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66T at cryogenic temperature |
| 4OL7 | 2015-01-28 | 1.67 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E A109E at cryogenic temperature |
| 1STY | 1993-04-15 | 1.67 | THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE |
| 4MIU | 2013-10-02 | 1.67 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V99T at cryogenic temperature |
| 4PNY | 2014-03-12 | 1.68 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I72H at cryogenic temperature |
| 4KY6 | 2013-06-12 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25D at pH 6 and cryogenic temperature |
| 4F7X | 2012-06-13 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L25A at cryogenic temperature |
| 2F0N | 2006-10-24 | 1.7 | Crystal structure of Staphylococcal nuclease mutant L25I/I72L |
| 1STG | 1995-01-26 | 1.7 | TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES |
| 5KYI | 2016-08-03 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 9 at cryogenic temperature |
| 4NMZ | 2013-12-11 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23Q/V66A at cryogenic temperature |
| 5IGG | 2016-03-09 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I72E at cryogenic temperature |
| 2EYL | 2006-10-24 | 1.7 | Crystal structure of Staphylococcal nuclease mutant T82S |
| 1STA | 1994-06-22 | 1.7 | ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE |
| 5F2D | 2015-12-16 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N/V99T at cryogenic temperature |
| 1STN | 1994-07-31 | 1.7 | THE CRYSTAL STRUCTURE OF STAPHYLOCOCCAL NUCLEASE REFINED AT 1.7 ANGSTROMS RESOLUTION |
| 3NP8 | 2011-06-29 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A at cryogenic temperature |
| 1EYD | 2000-10-18 | 1.7 | STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.7 A RESOLUTION |
| 2EY2 | 2006-10-24 | 1.7 | Crystal structure of Staphylococcal nuclease mutant T41C |
| 1EY5 | 2000-10-18 | 1.7 | STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V |
| 1SNQ | 1997-02-12 | 1.7 | PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE |
| 3SK5 | 2011-07-06 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D at cryogenic temperature |
| 2EYO | 2006-10-24 | 1.7 | Crystal structure of Staphylococcal nuclease mutant T120S |
| 5C5M | 2015-07-08 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D / V104E at cryogenic temperature |
| 2F0T | 2006-10-24 | 1.7 | Crystal structure of Staphylococcal nuclease mutant V66L/V99I |
| 1SNO | 1997-02-12 | 1.7 | PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE |
| 5IGF | 2016-03-09 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V66D at cryogenic temperature |
| 5DEH | 2015-09-02 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS N100D at cryogenic temperature |
| 4IZ8 | 2013-02-06 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS H8E at cryogenic temperature |
| 3H6M | 2010-03-09 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E at cryogenic temperature |
| 1SYD | 1994-07-31 | 1.7 | ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE |
| 4EQO | 2012-05-02 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V99D at cryogenic temperature |
| 4ZUI | 2015-05-27 | 1.7 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23H at cryogenic temperature |
| 1II3 | 2003-06-17 | 1.72 | Structure of S. nuclease quintuple mutant V23I/V66L/I72L/I92L/V99L |
| 1EY9 | 2000-10-18 | 1.72 | STRUCTURE OF S. NUCLEASE STABILIZING QUADRUPLE MUTANT T41I/P117G/H124L/S128A |
| 4ZUJ | 2015-05-27 | 1.72 | Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91H at cryogenic temperature |
| 3MVV | 2011-03-16 | 1.72 | Crystal structure of Staphylococcal nuclease variant Delta+PHS F34A at cryogenic temperature |
| 5C3W | 2015-07-15 | 1.72 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H at cryogenic temperature |
| 5J1Z | 2016-04-06 | 1.73 | Crystal structure of Staphylococcal nuclease variant Delta+PHS A90D at cryogenic temperature |
| 4ODG | 2014-01-29 | 1.73 | Crystal structure of Staphylococcal nuclease variant V23I/V66I/V74I/V99I at cryogenic temperature |
| 1SNM | 1991-07-15 | 1.74 | ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES |
| 4HMI | 2012-10-31 | 1.75 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V99K at cryogenic temperature |
| 3ITP | 2010-07-21 | 1.75 | Crystal structure of Staphylococcal nuclease variant Delta+PHS F34K at cryogenic temperature |
| 1EY6 | 2000-10-18 | 1.75 | STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T41I |
| 4HTH | 2012-11-28 | 1.75 | Crystal structure of Staphylococcal nuclease variant Delta+VIAGLA at cryogenic temperature |
| 5HGT | 2016-01-20 | 1.75 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92T at cryogenic temperature |
| 5C5L | 2015-07-08 | 1.75 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E / L125E at cryogenic temperature |
| 1EY8 | 2000-10-18 | 1.75 | STRUCTURE OF S. NUCLEASE STABILIZING TRIPLE MUTANT P117G/H124L/S128A |
| 5E3F | 2015-10-14 | 1.75 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K at cryogenic temperature |
| 2F0S | 2006-10-24 | 1.75 | Crystal structure of Staphylococcal nuclease mutant V66L/I92V |
| 4K5V | 2013-04-24 | 1.75 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L36A/V99T at cryogenic temperature |
| 4KD3 | 2013-05-08 | 1.75 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/V66A/I92A at cryogenic temperature |
| 5KIX | 2016-06-29 | 1.75 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92E at cryogenic temperature |
| 3P1H | 2011-10-05 | 1.79 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/I92A at cryogenic temperature |
| 5CV5 | 2015-08-12 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS K64G/V66K/E67G at cryogenic temperature |
| 5IGD | 2016-03-09 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperature |
| 5IGE | 2016-03-09 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V99D at cryogenic temperature |
| 4PMB | 2014-06-11 | 1.8 | Crystal structure of Staphylcoccal nuclease variant Delta+PHS I92S at cryogenic temperature |
| 3T16 | 2011-08-03 | 1.8 | Crystal structure of Staphylococcal nuclease variant NVIAGA/M98G at cryogenic temperature |
| 2EYP | 2006-10-24 | 1.8 | Crystal structure of Staphylococcal nuclease mutant T120V |
| 2F0J | 2006-10-24 | 1.8 | Crystal structure of Staphylococcal nuclease mutant I72V |
| 2EYF | 2006-10-24 | 1.8 | Crystal structure of Staphylococcal nuclease mutant T44V |
| 4ME5 | 2013-10-23 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S/V66A at cryogenic temperature |
| 1F2Z | 2003-09-16 | 1.8 | SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE |
| 3T13 | 2011-08-03 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G at cryogenic temperature |
| 3SK6 | 2011-07-06 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23K/L36E at cryogenic temperature |
| 1SYC | 1994-07-31 | 1.8 | ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE |
| 3BDC | 2008-11-04 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS at cryogenic temperature |
| 2F0I | 2006-10-24 | 1.8 | Crystal structure of Staphylococcal nuclease mutant I72L |
| 2EYM | 2006-10-24 | 1.8 | Crystal structure of Staphylococcal nuclease mutant T120C |
| 4QF4 | 2014-06-04 | 1.8 | Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature |
| 5IGB | 2016-03-09 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D/V66H at cryogenic temperature |
| 3TP6 | 2011-09-21 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/L103K at cryogenic temperature |
| 3DMU | 2008-11-04 | 1.8 | Crystal structure of Staphylococcal nuclease variant PHS T62K at cryogenic temperature |
| 5C4H | 2015-07-15 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92H at cryogenic temperature |
| 1KAB | 1994-01-31 | 1.8 | STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE |
| 5IGC | 2016-03-09 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/V66H at cryogenic temperature |
| 1SYE | 1994-07-31 | 1.8 | ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE |
| 3TP5 | 2011-09-21 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E/L36E at cryogenic temperature |
| 1SYB | 1994-07-31 | 1.8 | TRANSFER OF A BETA-TURN STRUCTURE TO A NEW PROTEIN CONTEXT |
| 3V2T | 2011-12-28 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V66A at cryogenic temperature |
| 6OK8 | 2019-05-08 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS K127L at cryogenic temperature |
| 5CR3 | 2015-08-05 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E/L125E at cryogenic temperature |
| 3HEJ | 2010-05-19 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature |
| 1SYF | 1994-07-31 | 1.8 | ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE |
| 5CV4 | 2015-08-12 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/V66H at cryogenic temperature |
| 4EQN | 2012-05-02 | 1.8 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/I72K at cryogenic temperature |
| 2F0U | 2006-10-24 | 1.83 | Crystal structure of Staphylococcal nuclease mutant V23I/L25I/I72V |
| 5K5P | 2016-06-01 | 1.83 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K L103K at cryogenic temperature |
| 1SND | 1997-04-21 | 1.84 | STAPHYLOCOCCAL NUCLEASE DIMER CONTAINING A DELETION OF RESIDUES 114-119 COMPLEXED WITH CALCIUM CHLORIDE AND THE COMPETITIVE INHIBITOR DEOXYTHYMIDINE-3',5'-DIPHOSPHATE |
| 5KYL | 2016-08-03 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 7 at cryogenic temperature |
| 1TT2 | 2004-07-06 | 1.85 | Cryogenic crystal structure of Staphylococcal nuclease variant truncated Delta+PHS I92K |
| 3ERO | 2008-11-04 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I72E at cryogenic temperature |
| 3OWF | 2010-10-20 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66R at cryogenic temperature |
| 1EZ8 | 2000-10-18 | 1.85 | STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V |
| 3SK4 | 2011-07-06 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N/V23E at cryogenic temperature |
| 2EYJ | 2006-10-24 | 1.85 | Crystal structure of Staphylococcal nuclease mutant T62V |
| 1EYC | 2000-10-18 | 1.85 | STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T41I/S59A/P117G/H124L/S128A |
| 5I9P | 2016-03-09 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H at cryogenic temperature |
| 2EY1 | 2006-10-24 | 1.85 | Crystal structure of Staphylococcal nuclease mutant T22V |
| 5IIF | 2016-03-09 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D at cryogenic temperature |
| 6AMF | 2017-09-06 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36E at cryogenic temperature |
| 1STH | 1995-02-27 | 1.85 | TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES |
| 5CGK | 2015-07-22 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66D/N100E at cryogenic temperature |
| 5EGT | 2015-11-11 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E at cryogenic temperature |
| 5C6A | 2015-07-08 | 1.85 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E / N100E at cryogenic temperature |
| 6OKA | 2019-05-08 | 1.86 | Crystal structure of Staphylococcal nuclease variant Delta+PHS Q123D at cryogenic temperature |
| 4RKB | 2014-11-26 | 1.88 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T/V99T at cryogenic temperature |
| 1EY7 | 2000-10-18 | 1.88 | STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S128A |
| 5JAV | 2016-04-27 | 1.89 | Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91D at cryogenic temperature |
| 2F0K | 2006-10-24 | 1.9 | Crystal structure of Staphylococcal nuclease mutant V23I/L25I |
| 1EZ6 | 2000-10-18 | 1.9 | STRUCTURE OF S. NUCLEASE STABILIZING SEXTUPLE MUTANT T33V/T41I/S59A/P117G/H124L/S128A |
| 3C1E | 2008-11-04 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L125K at cryogenic temperature |
| 5KGU | 2016-06-22 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS N118D at cryogenic temperature |
| 5J22 | 2016-04-06 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L103D at cryogenic temperature |
| 3NQT | 2011-06-29 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A at cryogenic temperature |
| 2F0G | 2006-10-24 | 1.9 | Crystal structure of Staphylococcal nuclease mutant V66I |
| 2F0O | 2006-10-24 | 1.9 | Crystal structure of Staphylococcal nuclease mutant V66I/I72V |
| 5I6W | 2016-02-24 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS A58D at cryogenic temperature |
| 3NUC | 1998-03-18 | 1.9 | STAPHLOCOCCAL NUCLEASE, 1-N-PROPANE THIOL DISULFIDE TO V23C VARIANT |
| 3D4W | 2009-01-06 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS A109R at cryogenic temperature |
| 4ID6 | 2013-03-06 | 1.9 | Crystal Structure of Staphylococcal nuclease mutant V23I/I72L |
| 3P75 | 2010-11-17 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V104D at cryogenic temperature |
| 5I6Y | 2016-02-24 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS A90E at cryogenic temperature |
| 5CC4 | 2015-07-15 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66E at cryogenic temperature |
| 1KAA | 1994-01-31 | 1.9 | STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE |
| 1KDA | 1995-02-27 | 1.9 | STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING |
| 1NUC | 1997-06-16 | 1.9 | STAPHYLOCOCCAL NUCLEASE, V23C VARIANT |
| 5ISR | 2016-03-23 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L38E at cryogenic temperature |
| 3QOL | 2011-03-16 | 1.9 | Crystal structure of Staphylococcal nuclease variant D+PHS/V23E at pH 6 determined at 100 K |
| 5E1F | 2015-10-14 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS G20E at cryogenic temperature |
| 4ZQ3 | 2015-05-27 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I92T at cryogenic temperature |
| 1SYG | 1994-07-31 | 1.9 | ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE |
| 3EJI | 2008-11-04 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K at cryogenic temperature |
| 3TP7 | 2011-09-21 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V99E at cryogenic temperature |
| 3SK8 | 2011-07-06 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G apo protein at cryogenic temperature |
| 2EXZ | 2006-10-24 | 1.9 | Crystal structure of Staphylococcal nuclease mutant T22C |
| 2EYH | 2006-10-24 | 1.9 | Crystal structure of Staphylococcal nuclease mutant T62S |
| 5IOC | 2016-03-23 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99D at cryogenic temperature |
| 1KDB | 1995-02-27 | 1.9 | STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING |
| 4KJO | 2013-05-15 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A/V66T/V99T at cryogenic temperature |
| 2F0L | 2006-10-24 | 1.9 | Crystal structure of Staphylococcal nuclease mutant V23L/I72L |
| 3R3O | 2011-03-30 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature and with high redundancy |
| 3S9W | 2011-06-22 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature |
| 2RBM | 2008-10-07 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K at cryogenic temperature |
| 6OK9 | 2019-05-08 | 1.9 | Crystal structure of Staphylococcal nuclease variant Delta+PHS K133M at cryogenic temperature |
| 2OF1 | 2008-03-25 | 1.92 | Cryogenic crystal structure of the Staphylococcal Nuclease variant truncated Delta+PHS I92W |
| 1EQV | 2003-07-08 | 1.94 | SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE |
| 2F0E | 2006-10-24 | 1.95 | Crystal structure of Staphylococcal nuclease mutant V23L |
| 3U9O | 2011-11-23 | 1.95 | Crystal structure of Staphylococcal nuclease variant NVIAGA/N100G at cryogenic temperature |
| 6EEG | 2018-08-29 | 1.95 | Crystal structure of Staphylcoccal nuclease variant Delta+PHS T62E at cryogenic temperature |
| 1ENC | 1994-05-31 | 1.95 | CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING |
| 5C3X | 2015-07-01 | 1.95 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D/N100E at cryogenic temperature |
| 5I9O | 2016-03-23 | 1.95 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K L103K at cryogenic temperature |
| 4EVO | 2012-05-23 | 1.95 | Crystal structure of Staphylococcal nuclease variant NVIAGA/E122G at cryogenic temperature |
| 1SNP | 1997-02-12 | 1.95 | PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE |
| 4KTA | 2013-06-12 | 1.95 | Crystal structure of Staphylococcal nuclease variant Delta+PHS R105Q at cryogenic temperatures |
| 5CV6 | 2015-08-12 | 1.95 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92E at cryogenic temperature |
| 2F0Q | 2006-10-24 | 1.95 | Crystal structure of Staphylococcal nuclease mutant V66L/I92L |
| 1A2T | 1998-04-29 | 1.96 | STAPHYLOCOCCAL NUCLEASE, B-MERCAPTOETHANOL DISULFIDE TO V23C VARIANT |
| 2SNM | 1993-01-15 | 1.97 | IN A STAPHYLOCOCCAL NUCLEASE MUTANT THE SIDE-CHAIN of A LYSINE REPLACING VALINE 66 IS FULLY BURIED IN THE HYDROPHOBIC CORE |
| 3D8G | 2009-01-06 | 1.99 | Crystal structure of Staphylococcal nuclease variant Delta+PHS I72R at cryogenic temperature |
| 2F0V | 2006-10-24 | 2.0 | Crystal structure of Staphylococcal nuclease mutant V23L/V66L/I72L |
| 3NHH | 2010-06-30 | 2.0 | Crystal structure of Staphylococcal nuclease variant V23E-L36K at cryogenic temperature |
| 2OEO | 2008-03-25 | 2.0 | Cryogenic crystal structure of Staphylococcal Nuclease variant truncated Delta+PHS I92D |
| 3C1F | 2008-11-04 | 2.0 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V104K at cryogenic temperature |
| 1KDC | 1995-02-27 | 2.0 | STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING |
| 3E5S | 2008-11-04 | 2.0 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature |
| 2F0M | 2006-10-24 | 2.0 | Crystal structure of Staphylococcal nuclease mutant V23L/I72V |
| 3EVQ | 2008-11-04 | 2.0 | Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E at cryogenic temperature |
| 1EYA | 2000-10-18 | 2.0 | STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T33V/T41I/P117G/H124L/S128A |
| 4K8J | 2013-05-01 | 2.0 | Crystal Structure of Staphylococcal nuclease mutant V23L/V66I |
| 2NUC | 1998-03-18 | 2.0 | STAPHLOCOCCAL NUCLEASE, ETHANE THIOL DISULFIDE TO V23C VARIANT |
| 1A2U | 1998-04-29 | 2.0 | STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 1-N-BUTANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE |
| 3D6C | 2008-11-04 | 2.0 | Crystal structure of Staphylococcal nuclease variant PHS L38E at cryogenic temperature |
| 1F2Y | 2003-09-16 | 2.0 | SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE |
| 2EY5 | 2006-10-24 | 2.0 | Crystal structure of Staphylococcal nuclease mutant T41S |
| 1F2M | 2003-09-16 | 2.0 | SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE |
| 1STB | 1994-07-31 | 2.0 | ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE |
| 4E6I | 2012-04-18 | 2.0 | Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature |
| 3HZX | 2010-05-19 | 2.0 | Crystal Structure of Staphylococcal nuclease variant D+PHS/V66K at pH 9 determined at 100 K |
| 1TQO | 2004-07-06 | 2.0 | Cryogenic Crystal Structure of Staphylococcal Nuclease Variant truncated Delta+PHS I92E |
| 2OXP | 2007-03-20 | 2.0 | Crystal Structure of Staphylococcal Nuclease mutant V66D/P117G/H124L/S128A |
| 2RDF | 2008-06-10 | 2.01 | Crystal Structure of staphyloccocal nuclease VIAGAN/E75A variant at cryogenic temperature |
| 2RKS | 2008-04-15 | 2.01 | Crystal structure of Staphylococcal nuclease variant PHS L38K at cryogenic temperature |
| 2ENB | 1994-05-31 | 2.05 | CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING |
| 2F0P | 2006-10-24 | 2.05 | Crystal structure of Staphylococcal nuclease mutant V66I/V99I |
| 1A3U | 1998-04-29 | 2.05 | STAPHYLOCOCCAL NUCLEASE, CYCLOHEXANE THIOL DISULFIDE TO V23C VARIANT |
| 5EKL | 2015-11-18 | 2.1 | Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D/N100E at cryogenic temperature |
| 1U9R | 2004-12-21 | 2.1 | Crystal Structure of Staphylococcal Nuclease mutant V66E/P117G/H124L/S128A at Room Temperature |
| 1TR5 | 2004-07-06 | 2.1 | Room temperature structure of Staphylococcal nuclease variant truncated Delta+PHS I92E |
| 3ERQ | 2008-11-04 | 2.1 | Crystal structure of Staphylococcal nuclease variant L25K at cryogenic temperature |
| 3D4D | 2008-11-04 | 2.1 | Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature |
| 1AEX | 1997-06-16 | 2.1 | STAPHYLOCOCCAL NUCLEASE, METHANE THIOL DISULFIDE TO V23C VARIANT |
| 5NUC | 1998-04-29 | 2.1 | STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT |
| 1A3T | 1998-04-29 | 2.1 | STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 2-FLUOROETHANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE |
| 5EKK | 2015-11-18 | 2.1 | Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D/L125E at cryogenic temperature |
| 2F0W | 2006-10-24 | 2.1 | Crystal structure of Staphylococcal nuclease mutant V23I/L25I/V66L/I72L |
| 4K8I | 2013-05-01 | 2.1 | Crystal Structure of Staphylococcal Nuclease mutant I92V/V99L |
| 3DHQ | 2009-01-06 | 2.15 | Crystal structure of Staphylococcal nuclease variant Delta+PHS A90R at cryogenic temperature |
| 1ENA | 1994-05-31 | 2.15 | CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING |
| 2F0F | 2006-10-24 | 2.15 | Crystal structure of Staphylococcal nuclease mutant L25I |
| 2F0H | 2006-10-24 | 2.15 | Crystal structure of Staphylococcal nuclease mutant V66L |
| 1A3V | 1998-04-29 | 2.2 | STAPHYLOCOCCAL NUCLEASE, CYCLOPENTANE THIOL DISULFIDE TO V23C VARIANT |
| 6EEK | 2018-08-29 | 2.2 | Crystal structure of apo Staphylcoccal nuclease variant Delta+PHS T62E/V66K, pH 7 at cryogenic temperature |
| 2QDB | 2008-07-01 | 2.2 | Crystal structure of staphylococcal nuclease variant E75Q/D21N/T33V/T41I/S59A/P117G/S128A at 100 K |
| 2F0D | 2006-10-24 | 2.5 | Crystal structure of Staphylococcal nuclease mutant I92V |
| 1NSN | 1995-09-15 | 2.8 | THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION |
| 2GSI | 2006-05-16 | 2.81 | Crystal Structure of a Murine Fab in Complex with an 11 Residue Peptide Derived from Staphylococcal Nuclease |
Relevant UniProtKB Entries
| Percent Identity | Matching Chains | Protein | Accession | Entry Name |
|---|---|---|---|---|
| 99.3 | Thermonuclease | Q6GIK1 | NUC_STAAR | |
| 99.3 | Thermonuclease | Q8NXI6 | NUC_STAAW | |
| 99.3 | Thermonuclease | Q6GB41 | NUC_STAAS | |
| 99.1 | Thermonuclease | Q7A6P2 | NUC_STAAN | |
| 99.1 | Thermonuclease | Q99VJ0 | NUC_STAAM | |
| 99.3 | Thermonuclease | Q5HHM4 | NUC_STAAC | |
| 100.0 | Thermonuclease | P00644 | NUC_STAAU |