On the thermal stability of alpha-crystallin: a new insight from infrared spectroscopy.


Abstract

alpha-Crystallin is a major structural protein of the vertebrate lens which shows structural and functional similarities to small heat shock proteins. The structure and the thermal stability of bovine alpha-crystallin were studied by Fourier-transform infrared spectroscopy, circular dichroism, and differential scanning calorimetry. Infrared spectroscopic data provide evidence which corroborates the view that the secondary structure of alpha-crystallin is highly ordered and consists predominantly of beta-sheets. However, the present results fail to support the widespread notion of an extremely high thermal stability of the protein. All three experimental approaches used in this study show that alpha-crystallin undergoes a major thermotropic transition with a midpoint at 60-62 degrees C. Furthermore, Fourier-transform infrared spectra provide evidence that this conformational transition is associated with a massive loss of the native beta-sheet structure. These results shed new light on structural properties of alpha-crystallin and have important implications for understanding the mechanism of the chaperone-like action of this protein. Study holds ProTherm entries: 5136 Extra Details: structural protein; beta-sheets; thermotropic transition;,chaperone-like

Submission Details

ID: bu7jw8dE

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:28 p.m.

Version: 1

Publication Details
Surewicz WK;Olesen PR,Biochemistry (1995) On the thermal stability of alpha-crystallin: a new insight from infrared spectroscopy. PMID:7626634
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3L1E 2010-05-12 1.15 Bovine AlphaA crystallin Zinc Bound
3L1F 2010-05-12 1.53 Bovine AlphaA crystallin

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
90.8 Alpha-crystallin A chain P02487 CRYAA_BRAVA
90.2 Alpha-crystallin A chain P02502 CRYAA_MACRU
91.9 Alpha-crystallin A chain P02486 CRYAA_CHOHO
90.8 Alpha-crystallin A chain P02501 CRYAA_ORYAF
92.5 Alpha-crystallin A chain P82533 CRYAA_ERIEU
92.5 Alpha-crystallin A chain P02499 CRYAA_PROCA
94.8 Alpha-crystallin A chain P02488 CRYAA_MACMU
94.2 Alpha-crystallin A chain P02498 CRYAA_LOXAF
94.2 Alpha-crystallin A chain P02489 CRYAA_HUMAN
94.2 Alpha-crystallin A chain A0A140G945 CRYA2_HUMAN
95.4 Alpha-crystallin A chain P02484 CRYAA_MANJA
96.0 Alpha-crystallin A chain P68406 CRYAA_TUPGL
96.0 Alpha-crystallin A chain P68405 CRYAA_MERUN
95.4 Alpha-crystallin A chain P68286 CRYAA_PERPO
95.4 Alpha-crystallin A chain P68287 CRYAA_OTOCR
96.0 Alpha-crystallin A chain P02492 CRYAA_OCHPR
96.0 Alpha-crystallin A chain P02483 CRYAA_NEOVI
96.5 Alpha-crystallin A chain P68288 CRYAA_ZALCA
96.5 Alpha-crystallin A chain P68289 CRYAA_HALGR
96.0 Alpha-crystallin A chain P02494 CRYAA_EULFU
97.1 Alpha-crystallin A chain P02482 CRYAA_ARTJA
96.5 Alpha-crystallin A chain P68283 CRYAA_PEDCA
96.5 Alpha-crystallin A chain P68281 CRYAA_CAVPO
97.1 Alpha-crystallin A chain P02477 CRYAA_PHOPH
97.1 Alpha-crystallin A chain P02480 CRYAA_MELUS
97.1 Alpha-crystallin A chain P02478 CRYAA_HORSE
97.1 Alpha-crystallin A chain P02493 CRYAA_RABIT
98.3 Alpha-crystallin A chain P68282 CRYAA_FELCA
98.3 Alpha-crystallin A chain P68280 CRYAA_CANLF
98.8 Alpha-crystallin A chain P02472 CRYAA_CAMDR
97.7 Alpha-crystallin A chain P02479 CRYAA_CERSI
97.7 Alpha-crystallin A chain P02475 CRYAA_PIG
98.3 Alpha-crystallin A chain P02474 CRYAA_BALAC
98.3 Alpha-crystallin A chain P02476 CRYAA_TAPIN
98.8 Alpha-crystallin A chain P82531 CRYAA_PTEPO
99.4 Alpha-crystallin A chain P68285 CRYAA_HIPAM
99.4 Alpha-crystallin A chain P68284 CRYAA_GIRCA
99.4 Alpha-crystallin A chain Q5ENZ0 CRYAA_SHEEP
100.0 Alpha-crystallin A chain P02470 CRYAA_BOVIN