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Reversible thermal unfolding of thermostable phosphoglycerate kinase. Thermostability associated with mean zero enthalpy change.

Abstract

Study holds ProTherm entries: 11202, 11203 Extra Details: thermodynamic parameters; two-state mechanism;,temperature-dependent; thermophilic

Submission Details

ID: agEDb6Dp

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:41 p.m.

Version: 1

Publication Details

Nojima H;Ikai A;Oshima T;Noda H,J. Mol. Biol. (1977) Reversible thermal unfolding of thermostable phosphoglycerate kinase. Thermostability associated with mean zero enthalpy change. PMID:338921

Additional Information

Study Summary

Number of data points	6
Proteins	Phosphoglycerate kinase ; Phosphoglycerate kinase ; Phosphoglycerate kinase ; Phosphoglycerate kinase
Unique complexes	2
Assays/Quantities/Protocols	Experimental Assay: Cm ; Experimental Assay: m ; Experimental Assay: dG_H2O
Libraries	Mutations for sequence MRTLLDLDPKGKRVLVRVDYNVPVQDGKVQDETRILESLPTLRHLLAGGASLVLLSHLGRPKGPDPKYSLAPVGEALRAHLPEARFAPFPPGSEEARREAEALRPGEVLLLENVRFEPGEEKNDPELSARYARLGEAFVLDAFGSAHRAHASVVGVARLLPAYAGFLMEKEVRALSRLLKDPERPYAVVLGGAKVSDKIGVIESLLPRIDRLLIGGAMAFTFLKALGGEVGRSLVEEDRLDLAKDLLGRAEALGVRVYLPEDVVAAERIEAGVETRVFPARAIPVPYMGLDIGPKTREAFARALEGARTVFWNGPMGVFEVPPFDEGTLAVGQAIAALEGAFTVVGGGDSVAAVNRLGLKERFGHVSTGGGASLEFLEKGTLPGLEVLEG ; Mutations for sequence SLSSKLSVQDLDLKDKRVFIRVDFNVPLDGKKITSNQRIVAALPTIKYVLEHHPRYVVLASHLGRPNGERNEKYSLAPVAKELQSLLGKDVTFLNDCVGPEVEAAVKASAPGSVILLENLRYHIEEEGSRKVDGQKVKASKEDVQKFRHELSSLADVYINDAFGTAHRAHSSMVGFDLPQRAAGFLLEKELKYFGKALENPTRPFLAILGGAKVADKIQLIDNLLDKVDSIIIGGGMAFTFKKVLENTEIGDSIFDKAVGPEIAKLMEKAKAKGVEVVLPVDFIIADAFSASANTKTVTDKEGIPAGWQGLDNGPESRKLFAATVAKATVILWNGPPGVFEFEKFAAGTKALLDEVVKSSAAGNTVIIGGGDTATVAKKYGVTDKISHVSTGGGASLELLEGKELPGVAFLSEKK

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Study data

Colors:	D	E	R	H	K	S	T	N	Q	A	V	I	L	M	F	Y	W	C	G	P

Data Distribution

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Relevant PDB Entries

Structure ID	Release Date	Resolution	Structure Title

Relevant UniProtKB Entries

Percent Identity	Protein	Accession	Entry Name
100.0	Phosphoglycerate kinase	P09403	PGK_THET8
99.0	Phosphoglycerate kinase	P62420	PGK_THET2
100.0	Phosphoglycerate kinase	P00560	PGK_YEAST
94.5	Phosphoglycerate kinase	Q6FKY1	PGK_CANGA