Engineering streptococcal protein G for increased alkaline stability.


Abstract

Most protein-based affinity chromatography media are very sensitive towards alkaline treatment, which is a preferred method for regeneration and removal of contaminants from the purification devices in industrial applications. In a previous study, we concluded that a simple and straightforward strategy consisting of replacing asparagine residues could improve the stability towards alkaline conditions. In this study, we have shown the potential of this rationale by stabilizing an IgG-binding domain of streptococcal protein G, i.e. the C2 domain. In order to analyze the contribution of the different amino acids to the alkaline sensitivity of the domain we used a single point mutation strategy. Amino acids known to be susceptible towards high pH, asparagine and glutamine, were substituted for less-alkali-susceptible residues. In addition, aspartic acid residues were mutated to evaluate if the stability could be further increased. The stability of the different C2 variants was subsequently analyzed by exposing them to NaOH. The obtained results reveal that the most sensitive amino acid towards alkaline conditions in the structure of C2 is Asn36. The double mutant, C2(N7,36A), was found to be the most stable mutant constructed. In addition to the increased alkaline stability and also very important for potential use as an affinity ligand, this mutated variant also retains the secondary structure, as well as the affinity to the Fc fragment of IgG.

Submission Details

ID: adGYZiDN4

Submitter: Shu-Ching Ou

Submission Date: Nov. 16, 2018, 1:54 p.m.

Version: 1

Publication Details
Gülich S;Linhult M;Ståhl S;Hober S,Protein Eng (2002) Engineering streptococcal protein G for increased alkaline stability. PMID:12468718
Additional Information

Study Summary

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
2N9K 2016-12-28 1H, 13C, and 15N Chemical Shift Assignments for in vitro GB1
2LUM 2012-08-29 Three-State Ensemble obtained from eNOEs of the Third Immunoglobulin Binding Domain of Protein G (GB3)
2IGG 1994-01-31 DETERMINATION OF THE SOLUTION STRUCTURES OF DOMAINS II AND III OF PROTEIN G FROM STREPTOCOCCUS BY 1H NMR
6HKA 2019-03-27 The solution structure of the micelle-associated FATC domain of the human protein kinase ataxia telangiectasia mutated (ATM)
1Q10 2003-10-14 Ensemble of 40 Structures of the Dimeric Mutant of the B1 Domain of Streptococcal Protein G
2MBB 2014-06-04 Solution Structure of the human Polymerase iota UBM1-Ubiquitin Complex
5UCE 2017-08-23 Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)
2N7J 2015-10-14 Sidechain chi1 distribution in B3 domain of protein G from extensive sets of residual dipolar couplings
1ZXH 2005-11-08 G311 mutant protein
1P7F 2003-08-05 GB3 solution structure obtained by refinement of X-ray structure with dipolar couplings
1GJS 2001-08-09 Solution structure of the Albumin binding domain of Streptococcal Protein G
1GB1 1993-04-15 A NOVEL, HIGHLY STABLE FOLD OF THE IMMUNOGLOBULIN BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G
6NJF 2019-08-21 Solution NMR Structure of DANCER3-F34A, a rigid and natively folded single mutant of the dynamic protein DANCER-3
2KHW 2010-02-23 Solution Structure of the human Polymerase iota UBM2-Ubiquitin Complex
2K0P 2009-03-03 Determination of a Protein Structure in the Solid State from NMR Chemical Shifts
2NMQ 2006-11-21 Simultaneous determination of protein structure and dynamics using rdcs
1P7E 2003-08-05 GB3 solution structure obtained by refinement of X-ray structure with dipolar couplings
1PN5 2003-10-07 NMR structure of the NALP1 Pyrin domain (PYD)
1LE3 2002-04-24 NMR Structure of Tryptophan Zipper 4: A Stable Beta-Hairpin Peptide Based on the C-terminal Hairpin of the B1 Domain of Protein G
3GB1 1999-06-23 STRUCTURES OF B1 DOMAIN OF STREPTOCOCCAL PROTEIN G
5UBS 2017-08-23 Solution NMR Structure of NERD-S, a natively folded pentamutant of the B1 domain of streptococcal protein G (GB1) with a solvent-exposed Trp43
1FD6 2001-09-19 DELTA0: A COMPUTATIONALLY DESIGNED CORE VARIANT OF THE B1 DOMAIN OF STREPTOCOCCAL PROTEIN G
2IGH 1994-01-31 DETERMINATION OF THE SOLUTION STRUCTURES OF DOMAINS II AND III OF PROTEIN G FROM STREPTOCOCCUS BY 1H NMR
2PLP 2007-05-08 Ultra high resolution backbone conformation of protein GB1 from residual dipolar couplings alone
1GB4 1998-07-22 HYPERTHERMOPHILIC VARIANT OF THE B1 DOMAIN FROM STREPTOCOCCAL PROTEIN G, NMR, 47 STRUCTURES
2KBT 2009-02-03 Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method
2N9L 2016-12-28 1H, 13C, and 15N Chemical Shift Assignments for in-cell GB1
2RPV 2009-09-15 Solution Structure of GB1 with LBT probe
2RMM 2007-12-04 Solution structure of GB1 A34F mutant
2RCJ 2008-01-22 Solution structure of human Immunoglobulin M
2KLK 2009-10-06 Solution structure of GB1 A34F mutant with RDC and SAXS
2I2Y 2006-12-12 Solution structure of the RRM of SRp20 bound to the RNA CAUC
2J52 2007-09-25 Solution Structure of GB1 domain Protein G and low and high pressure.
2LGI 2011-10-26 Atomic Resolution Protein Structures using NMR Chemical Shift Tensors
2KHU 2010-02-23 Solution Structure of the Ubiquitin-Binding Motif of Human Polymerase Iota
2KWD 2010-06-02 Supramolecular Protein Structure Determination by Site-Specific Long-Range Intermolecular Solid State NMR Spectroscopy
2J53 2007-09-25 Solution Structure of GB1 domain Protein G and low and high pressure.
5UCF 2017-08-23 Solution NMR-derived model of the minor species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)
5UB0 2017-08-23 Solution NMR Structure of NERD-C, a natively folded tetramutant of the B1 domain of streptococcal protein G (GB1)
2KN4 2010-08-18 The structure of the RRM domain of SC35
1GJT 2001-08-09 Solution structure of the Albumin binding domain of Streptococcal Protein G
2KQ4 2009-11-17 Atomic resolution protein structure determination by three-dimensional transferred echo double resonance solid-state nuclear magnetic resonance spectroscopy
2GB1 1993-04-15 A NOVEL, HIGHLY STABLE FOLD OF THE IMMUNOGLOBULIN BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G
2JU6 2007-12-04 Solid-State Protein Structure Determination with Proton-Detected Triple Resonance 3D Magic-Angle Spinning NMR Spectroscopy
1FCL 2001-09-19 DELTA1.5: A COMPUTATIONALLY DESIGNED CORE VARIANT OF THE B1 DOMAIN OF STREPTOCOCCAL PROTEIN G
2JSV 2008-04-15 Dipole tensor-based refinement for atomic-resolution structure determination of a nanocrystalline protein by solid-state NMR spectroscopy
1MPE 2002-10-30 Ensemble of 20 structures of the tetrameric mutant of the B1 domain of streptococcal protein G
3FIL 2009-08-18 0.88 Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution
2QMT 2007-12-25 1.05 Crystal Polymorphism of Protein GB1 Examined by Solid-state NMR and X-ray Diffraction
6CHE 2019-07-10 1.1 Selenomethionine mutant (A34Sem) of protein GB1 examined by X-ray diffraction
2IGD 1998-07-29 1.1 ANISOTROPIC STRUCTURE OF PROTEIN G IGG-BINDING DOMAIN III AT 1.1 ANGSTROM RESOLUTION
1IGD 1994-11-01 1.1 THE THIRD IGG-BINDING DOMAIN FROM STREPTOCOCCAL PROTEIN G: AN ANALYSIS BY X-RAY CRYSTALLOGRAPHY OF THE STRUCTURE ALONE AND IN A COMPLEX WITH FAB
6CPZ 2019-07-10 1.12 Selenomethionine mutant (I6Sem) of protein GB1 examined by X-ray diffraction
2GI9 2006-04-25 1.14 Backbone Conformational Constraints in a Microcrystalline U-15N-Labeled Protein by 3D Dipolar-Shift Solid-State NMR Spectroscopy
4LLD 2014-01-29 1.19 Structure of wild-type IgG1 antibody heavy chain constant domain 1 and light chain lambda constant domain (IgG1 CH1:Clambda) at 1.19A
6CNE 2019-07-10 1.2 Selenomethionine mutant (L5Sem) of protein GB1 examined by X-ray diffraction
6C9O 2019-07-10 1.2 Selenomethionine mutant (V29Sem) of protein GB1 examined by X-ray diffraction
3MP9 2011-02-23 1.2 Structure of Streptococcal protein G B1 domain at pH 3.0
6CTE 2019-07-10 1.2 77Se-NMR probes the protein environment of selenomethionine
3TV3 2011-10-19 1.29 Crystal structure of broad and potent HIV-1 neutralizing antibody PGT128 in complex with Man9
6NLA 2019-01-23 1.34 Crystal structure of de novo designed metal-controlled dimer of B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)-zinc
2ON8 2007-12-04 1.35 Gbeta1 stabilization by in vitro evolution and computational design
6NL6 2019-01-23 1.4 Crystal structure of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, N35K, D36H, N37Q)
6NL7 2019-01-23 1.4 Crystal structure of B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q)
4LLQ 2014-01-29 1.42 Structure of redesigned IgG1 first constant and lambda domains (CH1:Clambda constant redesign 2 beta, CRD2b) at 1.42A
6NL8 2019-01-23 1.5 Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, T16L, V29H, Y33H, N37L)-zinc
5HSF 2016-02-03 1.52 1.52 Angstrom Crystal Structure of Fc fragment of Human IgG1.
5BMH 2016-04-06 1.6 Nitroxide Spin Labels in Protein GB1: T44 Mutant, Crystal Form B
4NWU 2014-10-22 1.6 Crystal structure of APE1551, an anti-human NGF Fab with a nine amino acid insertion in CDR H1
1L6X 2002-04-10 1.65 FC FRAGMENT OF RITUXIMAB BOUND TO A MINIMIZED VERSION OF THE B-DOMAIN FROM PROTEIN A CALLED Z34C
3TWC 2011-10-19 1.65 Crystal structure of broad and potent HIV-1 neutralizing antibody PGT127 in complex with Man9
1PGX 1992-07-15 1.66 THE 1.66 ANGSTROMS X-RAY STRUCTURE OF THE B2 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON TO THE NMR STRUCTURE OF THE B1 DOMAIN
5JIH 2017-04-12 1.66 Crystal structure of HER2 binding IgG1-Fc (Fcab STAB19)
5U66 2017-05-24 1.7 Modified single helix from the B-domain of protein A bound to IgG1 Fc
3MCL 2010-11-24 1.7 Anti-beta-amyloid antibody c706 fab in space group P21
6NL9 2019-01-23 1.7 Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, T16L, V29H, Y33H, N37L)-apo
6ARP 2018-08-29 1.7 Structure of a mutant Cetuximab Fab fragment
2ONQ 2008-01-08 1.7 Gbeta1 stabilization by in vitro evolution and computational design
4LLM 2014-01-29 1.75 Structure of redesigned IgG1 first constant and lambda domains (CH1:Clambda constant redesign 1, CRD1) at 1.75A
4BSV 2013-08-21 1.75 Heterodimeric Fc Antibody Azymetric Variant 1
6N35 2018-11-28 1.75 Anti-HIV-1 Fab 2G12 + Man1-2 re-refinement
3DJ9 2008-09-16 1.75 Crystal Structure of an isolated, unglycosylated antibody CH2 domain
4NWT 2014-10-22 1.75 Crystal structure of the anti-human NGF Fab APE1531
4XMP 2015-04-29 1.78 Crystal structure of broadly and potently neutralizing antibody VRC08 in complex with HIV-1 clade A strain Q842.d12 gp120
5JII 2017-04-12 1.79 Crystal structure of human IgG1-Fc
1N7M 2003-02-04 1.8 Germline 7G12 with N-methylmesoporphyrin
5HYF 2017-02-01 1.8 Glycosylated Knob-Knob Fc fragment (P6122)
5W38 2017-10-18 1.8 1.80A resolution structure of human IgG3 Fc (N392K)
4W4O 2015-04-29 1.8 High-resolution crystal structure of Fc bound to its human receptor Fc-gamma-RI
4OZB 2014-07-16 1.8 Backbone Modifications in the Protein GB1 Helix: beta-ACPC24, beta-3-Lys28, beta-3-Lys31, beta-ACPC35
4W4N 2015-04-29 1.8 Crystal structure of human Fc at 1.80 A
5HG2 2016-02-24 1.8 Backbone Modifications in the Protein GB1 Helix: beta-3-Ala24, beta-3-Lys28, beta-3-Lys31, beta-2-Asn35
4B53 2012-12-05 1.8 Crystal structure of the isolated IgG4 CH3 domain
5JIK 2017-04-12 1.82 Crystal structure of HER2 binding IgG1-Fc (Fcab H10-03-6)
1AQK 1998-02-04 1.84 THREE-DIMENSIONAL STRUCTURE OF A HUMAN FAB WITH HIGH AFFINITY FOR TETANUS TOXOID
5W5N 2017-09-13 1.85 Crystal structure of human IgG4-Sigma2 Fc fragment
4O4Y 2014-03-26 1.85 Crystal structure of the anti-hinge rabbit antibody 2095-2 in complex with IDES hinge peptide
5GSQ 2017-06-28 1.85 Crystal structure of IgG Fc with a homogeneous glycoform and Antibody-Dependent Cellular Cytotoxicity
6G1E 2019-04-10 1.88 BEAT Fc with improved heterodimerization (Q3A-D84.4Q)
5HYE 2017-02-01 1.89 Glycosylated Knob-Knob Fc fragment (P212121)
4WI7 2015-09-30 1.9 Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation H435A
5DJZ 2016-03-30 1.9 Fc Heterodimer Design 7.8 D399M/Y407A + T366V/K409V
5DVL 2016-03-30 1.9 Fc Design 20.8.37 A chain homodimer Y349S/T366M/K370Y/K409V
4J12 2013-05-01 1.9 monomeric Fc
5DI8 2016-03-30 1.9 Fc Knob-Hole Heterodimer T366W + T366S/L368A/Y407V
4WI2 2015-09-30 1.9 Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc (wild-type)
5W5M 2017-09-13 1.9 Crystal structure of human IgG4-Sigma1 Fc fragment
6DCV 2018-06-06 1.9 Crystal structure of human anti-tau antibody CBTAU-27.1
4KU1 2014-02-19 1.9 Role of the hinge and C-gamma-2/C-gamma-3 interface in immunoglobin G1 Fc domain motions: implications for Fc engineering
5W5L 2017-09-13 1.9 Crystal structure of human IgG1-Sigma Fc fragment
4C54 2013-11-13 1.9 Crystal structure of recombinant human IgG4 Fc
3D85 2008-09-02 1.9 Crystal structure of IL-23 in complex with neutralizing FAB
4DZ8 2012-08-01 1.91 human IgG1 Fc fragment Heterodimer
4L4J 2013-08-28 1.92 Crystal structure of fc-fragment of human IgG2-Sigma antibody
1PGB 1994-04-30 1.92 TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCCOCAL PROTEIN G AND COMPARISON WITH NMR
5U52 2017-05-24 1.94 2 helix minimized version of the B-domain from Protein A (Z34C0 bound to IgG1 Fc (monoclinic form)
1QKZ 2000-02-06 1.95 Fab fragment (MN14C11.6) in complex with a peptide antigen derived from Neisseria meningitidis P1.7 serosubtype antigen and domain II from Streptococcal protein G
5HFY 2016-02-24 1.95 Backbone Modifications in the Protein GB1 Helix: beta-2-Ala24, beta-3-Lys28, beta-3-Lys31, beta-3-Asn35
4BM7 2013-07-03 1.95 Crystal Structure of IgG Fc F241A mutant with native glycosylation
1GAF 1996-07-11 1.95 48G7 HYBRIDOMA LINE FAB COMPLEXED WITH HAPTEN 5-(PARA-NITROPHENYL PHOSPHONATE)-PENTANOIC ACID
5HVW 2016-08-17 1.95 Monomeric IgG4 Fc
4KGS 2013-09-04 1.95 Backbone Modifications in the Protein GB1 Loops: beta-3-Val21, beta-3-Asp40
5M3V 2017-05-10 1.97 BEAT Fc
4EOW 2012-08-22 1.97 Crystal structure of a disease-associated anti-human GM-CSF autoantibody MB007
6FCZ 2018-02-28 10.0 Model of gC1q-Fc complex based on 7A EM map
4KGT 2013-09-04 2.0 Backbone Modifications in the Protein GB1 Turns: Aib10, D-Pro47
6MSY 2018-11-21 2.0 Anti-HIV-1 Fab Fab 2G12 + Man4 re-refinement
1D6V 2000-02-09 2.0 CONFORMATION EFFECTS IN BIOLOGICAL CATALYSIS INTRODUCED BY OXY-COPE ANTIBODY MATURATION
1EM7 2002-05-08 2.0 HELIX VARIANT OF THE B1 DOMAIN FROM STREPTOCOCCAL PROTEIN G
6DCW 2018-06-06 2.0 Crystal structure of human anti-tau antibody CBTAU-27.1 Fab in complex with a human tau peptide
1D5I 2000-02-09 2.0 UNLIGANDED GERMLINE PRECURSOR OF AN OXY-COPE CATALYTIC ANTIBODY
5DJ6 2016-03-30 2.0 Fc Heterodimer Design 6.1 F405W/Y407A + T366M
1VGE 1996-06-10 2.0 TR1.9 FAB FRAGMENT OF A HUMAN IGG1 KAPPA AUTOANTIBODY
3V3X 2012-08-29 2.0 Nitroxide Spin Labels in Protein GB1: N8/K28 Double Mutant
4KGR 2013-09-04 2.0 Backbone Modifications in the Protein GB1 Helix: beta-3-Ala24, beta-3-Lys28, beta-3-Lys31, beta-3-Asn35
3U0W 2012-10-03 2.0 AD related murine antibody Fragment
3AVE 2011-04-06 2.0 Crystal Structure of the Fucosylated Fc Fragment from Human Immunoglobulin G1
3V7M 2012-02-22 2.02 Crystal structure of monoclonal human anti-Rhesus D Fc IgG1 T125(YB2/0) in the presence of Zn2+
4HAF 2013-06-12 2.04 Crystal structure of fc-fragment of human IgG2 antibody (primitive crystal form)
5IW3 2017-03-29 2.05 anti-CD20 monoclonal antibody Fc fragment
1PGA 1994-04-30 2.07 TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON WITH NMR
5DJC 2016-03-30 2.1 Fc Heterodimer Design 8.1 L368V/Y407A + T366V/K409F
1AJ7 1997-11-12 2.1 IMMUNOGLOBULIN 48G7 GERMLINE FAB ANTIBODY COMPLEXED WITH HAPTEN 5-(PARA-NITROPHENYL PHOSPHONATE)-PENTANOIC ACID. AFFINITY MATURATION OF AN ESTEROLYTIC ANTIBODY
4NQT 2014-03-12 2.1 anti-parallel Fc-hole(T366S/L368A/Y407V) homodimer
2RCS 1997-11-12 2.1 IMMUNOGLOBULIN 48G7 GERMLINE FAB-AFFINITY MATURATION OF AN ESTEROLYTIC ANTIBODY
5DJY 2016-03-30 2.15 Fc Heterodimer Design 20.8 Y349S/T366V/K370Y/K409V + E357D/S364Q/Y407A
5O4E 2017-08-30 2.15 Crystal structure of VEGF in complex with heterodimeric Fcab JanusCT6
5HI1 2016-02-24 2.15 Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35
4BSW 2013-08-21 2.15 Heterodimeric Fc Antibody Azymetric Variant 2
3AGV 2010-11-10 2.15 Crystal structure of a human IgG-aptamer complex
4Q74 2014-07-23 2.19 F241A Fc
3AY4 2011-08-03 2.2 Crystal structure of nonfucosylated Fc complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa
4WI6 2015-09-30 2.2 Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation N434A
4OZA 2014-07-16 2.2 Backbone Modifications in the Protein GB1 Helix: beta-3-Ala24, beta-3-Lys28, beta-3-Gln32, beta-3-Asp36
4O51 2014-03-26 2.2 Crystal structure of the QAA variant of anti-hinge rabbit antibody 2095-2 in complex with IDES hinge peptide
1UWX 2005-06-15 2.2 P1.2 serosubtype antigen derived from N. meningitidis PorA in complex with Fab fragment
4WH4 2015-08-05 2.2 Protein GB1 Quadruple Mutant I6H/N8H/K28H/Q32H
3SGJ 2011-08-03 2.2 Unique carbohydrate-carbohydrate interactions are required for high affinity binding between FcgIII and antibodies lacking core fucose
6N32 2018-11-28 2.2 Anti-HIV-1 Fab 2G12 re-refinement
4HIX 2013-03-13 2.2 Crystal structure of a humanised 3D6 Fab bound to amyloid beta peptide
5BMG 2016-04-06 2.2 Nitroxide Spin Labels in Protein GB1: E15 Mutant
2DTS 2007-03-13 2.2 Crystal Structure of the Defucosylated Fc Fragment from Human Immunoglobulin G1
5DJX 2016-03-30 2.25 Fc Heterodimer Design 2.9 L368M/K370E + E357A/S364G
5VU0 2018-05-23 2.26 Crystal structure of the complex between afucosylated/galactosylated human IgG1 Fc and Fc gamma receptor IIIa (CD16A) with Man5 N-glycans
4ZNC 2015-07-15 2.28 Fc fragment of human IgG in complex with the C domain of staphylococcal protein A mutant - Q9W
5DJ0 2016-03-30 2.28 Fc Heterodimer Design 11.2 Y349S/K370Y + E357D/S364Q
6F2Z 2018-12-12 2.3 Structure of a Fc mutatnt
3D6G 2009-06-16 2.3 Fc fragment of IgG1 (Herceptin) with protein-A mimetic peptide dendrimer ligand.
5DK0 2016-03-30 2.3 Fc Heterodimer Design 20.8.34 Y349S/T366M/K370Y/K409V + E356G/E357D/S364Q/Y407A
5DJD 2016-03-30 2.3 Fc Heterodimer Design 5.1 T366V + Y407F
4CDH 2014-11-12 2.3 Crystallographic structure of the Human Igg1 alpha 2-6 sialilated Fc-Fragment
6NLB 2019-01-23 2.3 Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)-apo
1OQO 2003-03-20 2.3 Complex between G0 version of an Fc bound to a minimized version of Protein A called Mini-Z
1I7Z 2001-08-08 2.3 ANTIBODY GNC92H2 BOUND TO LIGAND
4BYH 2013-08-21 2.3 Crystal structure of sialylated IgG Fc
3C2S 2008-08-19 2.3 Structural Characterization of a Human Fc Fragment Engineered for Lack of Effector Functions
4XNY 2015-04-29 2.3 Crystal structure of broadly and potently neutralizing antibody VRC08C in complex with HIV-1 clade A strain Q842.d12 gp120
4OZC 2014-07-16 2.3 Backbone Modifications in the Protein GB1 Helix and Loops: beta-ACPC21, beta-ACPC24, beta-3-Lys28, beta-3-Lys31, beta-ACPC35, beta-ACPC40
5V43 2017-06-21 2.32 Engineered human IgG Fc domain aglyco801
6MU3 2018-10-31 2.33 Anti-HIV-1 Fab 2G12 + Man7 re-refinement
5IW6 2017-03-29 2.34 anti-CD20 monoclonal antibody Fc fragment
4C55 2013-11-13 2.35 Crystal structure of serum-derived human IgG4 Fc
4Q7D 2014-07-23 2.35 Wild type Fc (wtFc)
2IWG 2007-03-27 2.35 COMPLEX BETWEEN THE PRYSPRY DOMAIN OF TRIM21 AND IGG FC
4B7I 2012-10-17 2.36 Crystal Structure of Human IgG Fc Bearing Hybrid-type Glycans
5D4Q 2016-02-10 2.39 Crystal structure of GASDALIE IgG1 Fc
6D58 2019-05-15 2.39 Crystal structure of a Fc fragment of Human IgG3
1H3T 2003-01-23 2.4 Crystal structure of the human igg1 fc-fragment,glycoform (mn2f)2
6BZ4 2018-06-13 2.4 Human IgG1 lacking complement-dependent cytotoxicity: hu3S193 Fc mutant K322A
1H3U 2003-01-23 2.4 CRYSTAL STRUCTURE OF THE HUMAN IGG1 FC-FRAGMENT,GLYCOFORM (M3N2F)2
5DJ8 2016-03-30 2.4 Fc Heterodimer Design 7.7 D399M/Y407A + T366V/K409I
3SGK 2011-08-03 2.4 Unique carbohydrate/carbohydrate interactions are required for high affinity binding of FcgIII and antibodies lacking core fucose
5XJE 2017-11-01 2.4 Crystal structure of fucosylated IgG1 Fc complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa
4XXD 2015-04-29 2.41 Crystal Structure of mid-region amyloid beta capture by solanezumab
4ZNE 2015-11-11 2.42 IgG1 Fc-FcgammaRI ecd complex
5K64 2017-09-06 2.44 Crystal structure of VEGF binding IgG1-Fc (Fcab 448)
1H3X 2003-11-20 2.44 CRYSTAL STRUCTURE OF THE HUMAN IGG1 FC-FRAGMENT,GLYCOFORM (G0F)2
4ACP 2012-04-25 2.49 Deactivation of human IgG1 Fc by endoglycosidase treatment
6MUB 2018-10-31 2.5 Anti-HIV-1 Fab 2G12 + Man5 re-refinement
1PG7 2003-08-26 2.5 Murine 6A6 Fab in complex with humanized anti-Tissue Factor D3H44 Fab
5VZX 2017-08-09 2.5 Crystal structure of crenezumab Fab
1MVK 2002-10-30 2.5 X-ray structure of the tetrameric mutant of the B1 domain of streptococcal protein G
6FGO 2018-06-20 2.5 Fc in complex with engineered calcium binding domain Z
3DO3 2009-07-21 2.5 Human 1gG1 Fc fragment, 2.5 Angstrom structure
3FJT 2009-03-24 2.5 Crystal structure of a human Fc fragment engineered for extended serum half-life
4R26 2014-10-08 2.5 Crystal structure of human Fab PGT124, a broadly neutralizing and potent HIV-1 neutralizing antibody
5DVN 2016-03-30 2.5 Fc K392D/K409D homodimer
4X98 2015-06-03 2.5 Immunoglobulin Fc heterodimer variant
5DVO 2016-03-30 2.5 Fc K392D/K409D homodimer
5K65 2017-09-06 2.5 Crystal structure of VEGF binding IgG1-Fc (Fcab CT6)
5BMI 2016-04-06 2.5 Nitroxide Spin Labels in Protein GB1: T44 Mutant, Crystal Form A
5XJF 2017-11-01 2.5 Crystal structure of fucosylated IgG Fc Y296W mutant complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa
5U4Y 2017-05-17 2.5 IgG Fc bound to 3 helix of the B-domain from Protein A
4NQU 2014-03-12 2.5 anti-parallel Fc-knob (T366W) homodimer
4X99 2015-06-03 2.5 Immunoglobulin Fc heterodimers variant
2WAH 2009-03-10 2.51 Crystal Structure of an IgG1 Fc Glycoform (Man9GlcNAc2)
2QL1 2008-04-01 2.53 Structural Characterization of a Mutated, ADCC-Enhanced Human Fc Fragment
5DJ2 2016-03-30 2.56 Fc Heterodimer Design 7.4 Y407A + T366V/K409V
3U7W 2011-11-16 2.6 Crystal structure of NIH45-46 Fab
1IGC 1995-06-03 2.6 IGG1 FAB FRAGMENT (MOPC21) COMPLEX WITH DOMAIN III OF PROTEIN G FROM STREPTOCOCCUS
1OQX 2003-03-25 2.6 G-2 glycovariant of human IgG Fc bound to minimized version of Protein A called Z34C
5DVK 2016-03-30 2.6 Fc Design 7.7 B chain homodimer T366V/K409I
5DK2 2016-03-30 2.6 Fc Heterodimer E356K/D399K + K392D/K409D
3WJJ 2013-11-13 2.6 Crystal structure of IIb selective Fc variant, Fc(P238D), in complex with FcgRIIb
1AXS 1998-02-04 2.6 MATURE OXY-COPE CATALYTIC ANTIBODY WITH HAPTEN
5WAV 2017-11-08 2.6 Fc AbVance: Increasing our knowledge of antibody structural space to enable faster and better decision-making in antibody drug discovery.
4NQS 2014-03-12 2.64 Knob-into-hole IgG Fc
5Y56 2017-09-13 2.65 Fc mutant (K392D/K409D/D399K)
4WI9 2015-09-30 2.65 Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation I253A/H310A
1HKL 1997-03-12 2.68 FREE AND LIGANDED FORM OF AN ESTEROLYTIC CATALYTIC ANTIBODY
1DN2 2000-05-17 2.7 FC FRAGMENT OF HUMAN IGG1 IN COMPLEX WITH AN ENGINEERED 13 RESIDUE PEPTIDE DCAWHLGELVWCT-NH2
4D2N 2014-07-02 2.7 Crystal structure of deglycosylated serum-derived human IgG4 Fc
3V95 2012-02-22 2.7 Crystal structure of monoclonal human anti-rhesus D Fc and IgG1 t125(yb2/0) in the presence of EDTA
5TPS 2017-10-25 2.7 Structure of a Fc heterodimer
4WI3 2015-09-30 2.7 Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation I253A
5HY9 2017-02-01 2.7 Glycosylated, disulfide-linked Knob-into-Hole Fc fragment
5LG1 2016-12-14 2.7 Room temperature structure of human IgG4-Fc from crystals analysed in situ
1DFB 1993-10-31 2.7 STRUCTURE OF A HUMAN MONOCLONAL ANTIBODY FAB FRAGMENT AGAINST GP41 OF HUMAN IMMUNODEFICIENCY VIRUS TYPE I
1HZH 2001-08-15 2.7 CRYSTAL STRUCTURE OF THE INTACT HUMAN IGG B12 WITH BROAD AND POTENT ACTIVITY AGAINST PRIMARY HIV-1 ISOLATES: A TEMPLATE FOR HIV VACCINE DESIGN
5YC5 2018-03-21 2.71 Crystal structure of human IgG-Fc in complex with aglycan and optimized Fc gamma receptor IIIa
3V8C 2012-02-22 2.77 Crystal structure of monoclonal human anti-rhesus D Fc IgG1 t125(yb2/0) double mutant (H310 and H435 in K)
3WN5 2014-11-19 2.78 Crystal structure of asymmetrically engineered Fc variant in complex with FcgRIIIa
1D5B 2000-02-09 2.8 UNLIGANDED MATURE OXY-COPE CATALYTIC ANTIBODY
4WI5 2015-09-30 2.8 Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation H310A
3UI3 2012-02-08 2.8 Structural and Biochemical Characterization of HP0315 from Helicobacter pylori as a VapD Protein with an Endoribonuclease Activity
5XMH 2018-03-21 2.8 Crystal structure of an IgM rheumatoid factor YES8c in complex with IgG1 Fc
3O11 2010-09-08 2.8 Anti-beta-amyloid antibody c706 fab in space group c2
4WI4 2015-09-30 2.8 Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation S254A
4WI8 2015-09-30 2.8 Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation Y436A
1FC2 1981-10-02 2.8 Crystallographic Refinement and Atomic Models of a Human FC Fragment and its Complex with Fragment B of Protein A from Staphylococcus Aureus at 2.9-and 2.8-Angstroms Resolution
3DNK 2009-07-21 2.84 Enzyme deglycosylated Human IgG1 Fc fragment
1H3W 2003-01-23 2.85 Structural analysis of human IgG-Fc glycoforms reveals a correlation between glycosylation and structural integrity
3WJL 2013-11-13 2.86 Crystal structure of IIb selective Fc variant, Fc(V12), in complex with FcgRIIb
1FC1 1981-10-02 2.9 CRYSTALLOGRAPHIC REFINEMENT AND ATOMIC MODELS OF A HUMAN FC FRAGMENT AND ITS COMPLEX WITH FRAGMENT B OF PROTEIN A FROM STAPHYLOCOCCUS AUREUS AT 2.9-AND 2.8-ANGSTROMS RESOLUTION
5DJA 2016-03-30 2.9 Fc Heterodimer Design 9.1 Y407M + T366I
5HYI 2017-02-01 2.9 Glycosylated, disulfide-linked Hole-Hole Fc fragment
3WKN 2014-10-29 2.9 Crystal structure of the artificial protein AFFinger p17 (AF.p17) complexed with Fc fragment of human IgG
5DVM 2016-03-30 2.95 Fc Design 20.8.37 B chain homodimer E357D/S364R/Y407A
2J6E 2007-04-10 3.0 Crystal Structure of an Autoimmune Complex between a Human IgM Rheumatoid Factor and IgG1 Fc reveals a Novel Fc Epitope and Evidence for Affinity Maturation
6N2X 2018-11-28 3.0 Anti-HIV-1 Fab 2G12 + Man9 re-refinement
5BW7 2015-10-14 3.0 Crystal structure of nonfucosylated Fc Y296W mutant complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa
4Q6Y 2014-07-23 3.0 Crystal structure of a chemoenzymatic glycoengineered disialylated Fc (di-sFc)
6IQG 2019-02-13 3.0 X-ray crystal structure of Fc and peptide complex
6IQH 2019-02-13 3.0 X-ray crystal structure of covalent-bonded complex of Fc and peptide
1T83 2004-09-28 3.0 CRYSTAL STRUCTURE OF A HUMAN TYPE III FC GAMMA RECEPTOR IN COMPLEX WITH AN FC FRAGMENT OF IGG1 (ORTHORHOMBIC)
3EO1 2008-12-02 3.1 Structure of the Fab Fragment of GC-1008 in Complex with Transforming Growth Factor-Beta 3
1H3V 2003-01-23 3.1 CRYSTAL STRUCTURE OF THE HUMAN IGG1 FC-FRAGMENT,GLYCOFORM (G2F)2,SG P212121
1BBJ 1994-01-31 3.1 CRYSTAL STRUCTURE OF A CHIMERIC FAB' FRAGMENT OF AN ANTIBODY BINDING TUMOUR CELLS
3S7G 2012-05-30 3.13 Aglycosylated human igg1 fc fragment
5D6D 2016-02-10 3.13 Crystal structure of GASDALIE IgG1 Fc in complex with FcgRIIIa
1ADQ 1998-09-16 3.15 CRYSTAL STRUCTURE OF A HUMAN IGM RHEUMATOID FACTOR FAB IN COMPLEX WITH ITS AUTOANTIGEN IGG FC
1E4K 2000-08-06 3.2 CRYSTAL STRUCTURE OF SOLUBLE HUMAN IGG1 FC FRAGMENT-FC-GAMMA RECEPTOR III COMPLEX
6OKQ 2019-06-05 3.2 Crystal structure of the SF12 Fab
1FCC 1995-04-20 3.2 CRYSTAL STRUCTURE OF THE C2 FRAGMENT OF STREPTOCOCCAL PROTEIN G IN COMPLEX WITH THE FC DOMAIN OF HUMAN IGG
5V4E 2017-06-21 3.22 Engineered human IgG Fc domain glyco801 (Fc801)
3TYG 2011-10-19 3.25 Crystal structure of broad and potent HIV-1 neutralizing antibody PGT128 in complex with a glycosylated engineered gp120 outer domain with miniV3 (eODmV3)
5K33 2017-04-12 3.3 Crystal structure of extracellular domain of HER2 in complex with Fcab STAB19
5LDE 2017-10-04 3.38 Crystal structure of a vFLIP-IKKgamma stapled peptide dimer
4XNZ 2015-04-29 3.39 Crystal structure of broadly and potently neutralizing antibody VRC06B in complex with HIV-1 clade A/E strain 93TH057 gp120
4HAG 2013-06-12 3.4 Crystal structure of fc-fragment of human IgG2 antibody (centered crystal form)
4X4M 2015-04-08 3.48 Structure of FcgammaRI in complex with Fc reveals the importance of glycan recognition for high affinity IgG binding
1T89 2004-09-14 3.5 CRYSTAL STRUCTURE OF A HUMAN TYPE III FC GAMMA RECEPTOR IN COMPLEX WITH AN FC FRAGMENT OF IGG1 (HEXAGONAL)
4N0U 2014-02-05 3.8 Ternary complex between Neonatal Fc receptor, serum albumin and Fc
3RY6 2011-08-31 3.8 Complex of fcgammariia (CD32) and the FC of human IGG1
1H3Y 2003-01-23 4.1 Crystal structure of a human IgG1 Fc-fragment,high salt condition
6DE7 2018-06-06 4.12 Crystal Structure at 4.3 A Resolution of Glycosylated HIV-1 Clade A BG505 SOSIP.664 Prefusion Env Trimer with Interdomain Stabilization 113C-429GCG in Complex with Broadly Neutralizing Antibodies PGT122 and 35O22
5K8D 2017-06-14 4.19 Crystal structure of rFVIIIFc
5KWG 2017-04-12 4.3 Crystal structure of extracellular domain of HER2 in complex with Fcab H10-03-6
2GJ7 2006-05-30 5.0 Crystal Structure of a gE-gI/Fc complex

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
192.8 C,D Immunoglobulin heavy constant gamma 1 P06654 SPG1_STRSG
200.0 C,D Immunoglobulin heavy constant gamma 1 P19909 SPG2_STRSG
185.4 A,B Immunoglobulin heavy constant gamma 1 P01860 IGHG3_HUMAN
186.4 A,B Immunoglobulin heavy constant gamma 1 P01861 IGHG4_HUMAN
187.4 A,B Immunoglobulin heavy constant gamma 1 P01859 IGHG2_HUMAN
193.2 A,B Immunoglobulin heavy constant gamma 1 P01857 IGHG1_HUMAN
193.2 A,B Immunoglobulin heavy constant gamma 1 P0DOX5 IGG1_HUMAN