Extremely rapid protein folding in the absence of intermediates.


Abstract

Here we used the cold-shock protein CspB from Bacillus subtilis to study protein folding at an elementary level. The thermodynamic stability of this small five-stranded beta-barrel protein is low, but unfolding and refolding are extremely rapid reactions. In 0.6 M urea the time constant of refolding is about 1.5 ms, and at the transition midpoint (4 M urea) the folded and unfolded forms equilibrate in less than 100 ms. Both the equilibrium unfolding transition and the folding kinetics are perfectly described by a N<-->U two-state model. The validity of this model was confirmed by several kinetic tests. Folding intermediates could neither be detected at equilibrium nor in the folding kinetics. We suggest that the extremely rapid folding of CspB and the absence of folding intermediates are related phenomena. Study holds ProTherm entries: 9329 Extra Details: cold-shock protein CspB; thermodynamic stability;,time constant of refolding; folding intermediates

Submission Details

ID: Z8QdExir3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:37 p.m.

Version: 1

Publication Details
Schindler T;Herrler M;Marahiel MA;Schmid FX,Nat. Struct. Biol. (1995) Extremely rapid protein folding in the absence of intermediates. PMID:7552728
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1NMF 1996-07-11 MAJOR COLD-SHOCK PROTEIN, NMR, 20 STRUCTURES
1NMG 1996-07-11 MAJOR COLD-SHOCK PROTEIN, NMR, MINIMIZED AVERAGE STRUCTURE
2F52 2006-09-19 Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine
3PF4 2011-09-21 1.38 Crystal structure of Bs-CspB in complex with r(GUCUUUA)
3PF5 2011-09-21 1.68 Crystal structure of Bs-CspB in complex with rU6
2ES2 2006-09-05 1.78 Crystal Structure Analysis of the Bacillus Subtilis Cold Shock Protein Bs-CspB in Complex with Hexathymidine
2I5M 2007-05-22 2.3 Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R
1CSP 1995-05-12 2.45 CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS MAJOR COLD SHOCK PROTEIN, CSPB: A UNIVERSAL NUCLEIC-ACID BINDING DOMAIN
2I5L 2007-05-22 2.55 Crystal structure of Bacillus subtilis Cold Shock Protein variant Bs-CspB M1R/E3K/K65I
1CSQ 1995-05-12 2.7 CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS MAJOR COLD SHOCK PROTEIN, CSPB: A UNIVERSAL NUCLEIC-ACID BINDING DOMAIN

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
96.5 Cold shock protein CspB P41018 CSPB_SPOGL
92.3 Cold shock protein CspB Q816H3 CSPD_BACCR
92.3 Cold shock protein CspB Q81K90 CSPD_BACAN
100.0 Cold shock protein CspB P32081 CSPB_BACSU