Effect of H helix destabilizing mutations on the kinetic and equilibrium folding of apomyoglobin.


Abstract

Acid-denatured apomyoglobin (apoMb) contains residual helical structure in the region of the polypeptide which corresponds to the H helix of the folded protein. In order to elucidate the role of this residual secondary structure in the protein folding process and to determine whether residual structure in the denatured state affects either the overall rate of folding or the rate of formation of a burst phase intermediate, we have examined the equilibrium and kinetic folding behavior of a mutant designed to destabilize residual secondary structure in the H helix region. Both Asn132 and Glu136 were changed to Gly (N132G,E136G) to effect this destabilization. Circular dichroism spectra show that the mutant protein contains less helical structure in the acid-denatured state and in the equilibrium intermediate state at pH 4.2 than does the wild-type protein. The CD spectra of the native states of the two proteins are nearly identical. The refolding kinetics for each of the species were measured by stopped-flow CD in the far-UV region and by NMR quench-flow pulse labeling. Under identical conditions, the CD-detected refolding of wild-type and mutant apomyoglobin from the acid-denatured state or from the urea-denatured state occurs at very similar rates following a burst phase that occurs too rapidly to measure by the stopped-flow technique. The urea dependence of the unfolding and refolding rates is consistent with the presence of at least one obligatory on-pathway intermediate in both wild-type and mutant proteins. The kinetic intermediate of the mutant protein is considerably less stable than that of the wild-type protein. Hydrogen exchange pulse labeling experiments indicate that, in contrast to the wild-type protein, the H helix is not stabilized during the burst phase refolding of the mutant but becomes stabilized during the slower phases. While the wild-type and mutant proteins both form compact intermediates, these differ in the content and location of secondary structure. The rate of folding of the AGH subdomain, which takes place prior to the transition state, is substantially slower for the N132G,E136G mutant protein. A strong propensity for spontaneous formation of helical structure in the H helix region is not a prerequisite for efficient folding nor for formation of equilibrium or kinetic intermediates. These observations suggest that while folding of apomyoglobin proceeds through an obligatory intermediate, the precise structure of this intermediate is not critical and its secondary structure may be altered without substantially affecting either the overall refolding kinetics or the integrity of the final folded state. Study holds ProTherm entries: 6215, 6216 Extra Details: the transition is from unfolding to intermediate myoglobin; protein folding; NMR hydrogen-deuterium exchange;,protein folding intermediate

Submission Details

ID: YPvTY2rd

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:31 p.m.

Version: 1

Publication Details
Cavagnero S;Dyson HJ;Wright PE,J. Mol. Biol. (1999) Effect of H helix destabilizing mutations on the kinetic and equilibrium folding of apomyoglobin. PMID:9878405
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1MYF 1995-02-27 SOLUTION STRUCTURE OF CARBONMONOXY MYOGLOBIN DETERMINED FROM NMR DISTANCE AND CHEMICAL SHIFT CONSTRAINTS
5YCE 2018-09-19 0.77 Sperm whale myoglobin swMb
1A6M 1999-04-06 1.0 OXY-MYOGLOBIN, ATOMIC RESOLUTION
1NAZ 2003-06-10 1.04 structure of microgravity-grown oxidized myoglobin mutant YQR (ISS8A)
6M8F 2019-01-23 1.1 Engineered sperm whale myoglobin-based carbene transferase
1A6K 1999-04-06 1.1 AQUOMET-MYOGLOBIN, ATOMIC RESOLUTION
3O89 2011-12-07 1.1 Crystal Structure of Sperm Whale Myoglobin G65T
2EKT 2007-08-14 1.1 Crystal structure of myoglobin reconstituted with 6-methyl-6-depropionatehemin
4H07 2012-11-21 1.14 Complex of G65T Myoglobin with Phenol in its Proximal Cavity
1BZR 1999-05-10 1.15 ATOMIC RESOLUTION CRYSTAL STRUCTURE ANALYSIS OF NATIVE DEOXY AND CO MYOGLOBIN FROM SPERM WHALE AT ROOM TEMPERATURE
1BZP 1999-05-10 1.15 ATOMIC RESOLUTION CRYSTAL STRUCTURE ANALYSIS OF NATIVE DEOXY AND CO MYOGLOBIN FROM SPERM WHALE AT ROOM TEMPERATURE
1A6G 1998-10-21 1.15 CARBONMONOXY-MYOGLOBIN, ATOMIC RESOLUTION
1A6N 1999-04-06 1.15 DEOXY-MYOGLOBIN, ATOMIC RESOLUTION
1U7R 2005-07-19 1.15 Crystal structure of Native Sperm Whale myoglobin from low ionic strength enviroment (Form2 )
2Z6T 2007-11-06 1.2 Crystal structure of the ferric peroxo myoglobin
1BZ6 1999-05-10 1.2 ATOMIC RESOLUTION CRYSTAL STRUCTURE AQUOMET-MYOGLOBIN FROM SPERM WHALE AT ROOM TEMPERATURE
3EDB 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 120 K: Laser on [150 min]
2ZSQ 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 140 K: Laser on [150 min]
2ZSZ 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 100 K: Laser on [600 min]
2ZSY 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 140 K: Laser on [750 min]
2ZT0 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 100 K: Laser on [750 min]
2ZT1 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 100 K: Laser on [810 min]
3ECL 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 40 K: Laser on
3EDA 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 100 K: Laser on [150 min]
2ZT3 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 120 K: Laser on [750 min]
3E5O 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 140 K: Laser off
2ZSO 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 100 K: Laser on [450 min]
3ECZ 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 120 K: Laser on [30 min]
3U3E 2012-11-28 1.21 Complex of Wild Type Myoglobin with Phenol in its Proximal Cavity
2ZT2 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 120 K: Laser on [600 min]
2ZST 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 140 K: Laser on [450 min]
3E55 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 100 K: Laser off
3ECX 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 100 K: Laser on [30 min]
2ZT4 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 120 K: Laser on [810 min]
2ZSR 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 120 K: Laser on [450 min]
2ZSP 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 120 K: Laser on [300 min]
3ED9 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 140 K: Laser on [30 min]
2ZSN 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 100 K: Laser on [300 min]
2ZSS 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 140 K: Laser on [300 min]
2ZSX 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 140 K: Laser on [600 min]
3E4N 2009-02-24 1.21 Carbonmonoxy Sperm Whale Myoglobin at 40 K: Laser off
3E5I 2009-02-24 1.22 Carbonmonoxy Sperm Whale Myoglobin at 120 K: Laser off
4OOD 2014-11-26 1.24 Structure of K42Y mutant of sperm whale myoglobin
4OF9 2014-11-26 1.24 Structure of K42N variant of sperm whale myoglobin
5OJC 2018-01-24 1.25 Structure of MbQ2.1 NMH
2Z6S 2007-11-06 1.25 Crystal structure of the oxy myoglobin free from X-ray-induced photoreduction
5XL0 2017-08-16 1.25 met-aquo form of sperm whale myoglobin reconstituted with 7-PF, a heme possesseing CF3 group as side chain
4H0B 2012-11-21 1.26 Complex of G65T Myoglobin with DMSO in its Distal Cavity
5HAV 2016-01-13 1.27 Sperm whale myoglobin mutant L29H F33Y F43H (F33Y CuBMb) with oxygen bound
1JW8 2001-10-10 1.3 1.3 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF P6 FORM OF MYOGLOBIN
5OJA 2018-01-24 1.35 Structure of MbQ
5YCH 2018-09-19 1.35 Ancestral myoglobin aMbWb of Basilosaurus relative (monophyly)
4PNJ 2014-11-05 1.36 Recombinant Sperm Whale P6 Myoglobin Solved with Single Pulse Free Electron Laser Data
4LPI 2014-07-16 1.36 A sperm whale myoglobin double mutant L29H/F43Y Mb with a distal hydrogen-bonding network
3M3A 2010-05-12 1.37 The roles of glutamates and metal ions in a rationally designed nitric oxide reductase based on myoglobin: Cu(II)-I107E FeBMb (Cu(II) binding to FeB site)
1DXD 2000-03-27 1.4 Photolyzed CO complex of Myoglobin Mb-YQR at 20K
2EVK 2006-04-18 1.4 The Structures of Thiolate- and Carboxylate-Ligated Ferric H93G Myoglobin: Models for Cytochrome P450 and for Oxyanion-Bound Heme Proteins
5XKV 2018-05-16 1.4 structure of sperm whale myoglobin F138W
1U7S 2005-07-19 1.4 Crystal structure of Native Sperm Whale myoglobin from low ionic strength enviroment (Form 1)
1BVD 1995-07-31 1.4 STRUCTURE OF A BILIVERDIN APOMYOGLOBIN COMPLEX (FORM B) AT 98 K
2EKU 2007-08-14 1.4 Crystal structure of myoglobin reconstituted with 7-methyl-7-depropionatehemin
2JHO 2007-04-03 1.4 Cyanomet Sperm Whale Myoglobin at 1.4A resolution
1H1X 2003-10-23 1.4 Sperm whale Myoglobin mutant T67R S92D
1DXC 2000-04-02 1.4 CO complex of Myoglobin Mb-YQR at 100K
1MBD 1982-02-03 1.4 X-ray structure of sperm whale deoxymoglobin refined at 1.4A resolution
3M38 2010-05-12 1.42 The roles of Glutamates and Metal ions in a rationally designed nitric oxide reductase based on myoglobin: I107E FeBMb (No metal ion binding to FeB site)
1V9Q 2005-05-17 1.45 Crystal Structure of an Artificial Metalloprotein:Mn(III)(3,3'-Me2-salophen)/apo-A71G Myoglobin
6F17 2018-08-22 1.45 Structure of Mb NMH H64V, V68A mutant resting state
1J3F 2004-05-18 1.45 Crystal Structure of an Artificial Metalloprotein:Cr(III)(3,3'-Me2-salophen)/apo-A71G Myoglobin
4PQ6 2015-01-14 1.45 A sperm whale myoglobin single mutant L29E Mb with native His93 coordination
6G5A 2018-08-22 1.48 Heme-carbene complex in myoglobin H64V/V68A containing an N-methylhistidine as the proximal ligand, 1.48 angstrom resolution
5OJ9 2018-01-24 1.48 Structure of Mb NMH
4PQC 2015-01-14 1.5 A sperm whale myoglobin single mutant F43H Mb with native His93 coordination
5HLQ 2016-05-25 1.5 X-ray crystal structure of met F43H/H64A sperm whale myoglobin
1L2K 2002-08-21 1.5 Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.
4MXL 2014-02-12 1.5 X-ray structure of ZnPFeBMb1
5HLX 2016-05-25 1.5 X-ray crystal structure of met F43H/H64A sperm whale myoglobin in complex with nitrite
2MBW 1996-12-23 1.5 RECOMBINANT SPERM WHALE MYOGLOBIN (MET)
1MBC 1989-01-09 1.5 X-RAY STRUCTURE AND REFINEMENT OF CARBON-MONOXY (FE II)-MYOGLOBIN AT 1.5 ANGSTROMS RESOLUTION
3SDN 2011-11-02 1.5 Structure of G65I sperm whale myoglobin mutant
1BVC 1995-07-31 1.5 STRUCTURE OF A BILIVERDIN APOMYOGLOBIN COMPLEX (FORM D) AT 118 K
6G5T 2018-08-22 1.5 Myoglobin H64V/V68A in the resting state, 1.5 Angstrom resolution
1ABS 1997-04-01 1.5 PHOTOLYSED CARBONMONOXY-MYOGLOBIN AT 20 K
4IT8 2013-04-10 1.5 A sperm whale myoglobin mutant L29H Mb with two distal histidines
1DO1 2000-01-05 1.5 CARBONMONOXY-MYOGLOBIN MUTANT L29W AT 105K
5HLU 2016-05-25 1.5 X-ray crystal structure of met F43H/H64A sperm whale myoglobin in complex with nitric oxide
4MXK 2014-02-12 1.52 X-ray structure of Fe(II)-ZnPIXFeBMb1
1WVP 2005-01-18 1.53 Structure of chemically modified myoglobin with distal N-tetrazolyl-histidine E7(64)
5OJB 2018-01-24 1.54 Structure of MbQ NMH
1DO3 2000-01-05 1.55 CARBONMONOXY-MYOGLOBIN (MUTANT L29W) AFTER PHOTOLYSIS AT T>180K
4NXC 2014-09-24 1.55 SPERM WHALE MYOGLOBIN UNDER 30 BAR NITROUS Oxide
5VNU 2017-08-16 1.58 Nonheme Iron Replacement in a Biosynthetic Nitric Oxide Reductase Model Performing O2 Reduction to Water: Mn-bound FeBMb
5YCJ 2018-09-19 1.58 Ancestral myoglobin aMbWb' of Basilosaurus relative (polyphyly) imidazole-ligand
1UFJ 2004-05-18 1.6 Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-A71G Myoglobin
1FCS 1993-10-31 1.6 CRYSTAL STRUCTURE OF A DISTAL SITE DOUBLE MUTANT OF SPERM WHALE MYOGLOBIN AT 1.6 ANGSTROMS RESOLUTION
2W6Y 2009-12-22 1.6 Crystal structure of Sperm Whale Myoglobin mutant YQR in complex with Xenon
1N9X 2003-06-10 1.6 structure of microgravity-grown oxidized myoglobin mutant YQR (ISS8A)
6G5B 2018-08-22 1.6 Heme-carbene complex in myoglobin H64V/V68A containing an N-methylhistidine as the proximal ligand, 1.6 angstrom resolution
1CO9 1999-06-14 1.6 RECOMBINANT SPERM WHALE MYOGLOBIN L104V MUTANT (MET)
1MYZ 2003-08-19 1.6 CO COMPLEX OF MYOGLOBIN MB-YQR AT RT SOLVED FROM LAUE DATA.
3OGB 2010-09-08 1.6 Sperm whale myoglobin mutant H64W deoxy-form
1MBO 1982-02-03 1.6 Structure and refinement of oxymyoglobin at 1.6 angstroms resolution
2E2Y 2007-10-23 1.6 Crystal Structure of F43W/H64D/V68I Myoglobin
1CIO 1999-04-09 1.6 RECOMBINANT SPERM WHALE MYOGLOBIN I99V MUTANT (MET)
5ZZF 2018-10-03 1.6 X-ray structure of F43Y/H64D sperm whale myoglobin
4NXA 2014-09-24 1.6 SPERM WHALE MYOGLOBIN UNDER XENON PRESSURE 30 Bar
4QAU 2015-01-21 1.6 Crystal structure of F43Y mutant of sperm whale myoglobin
3M3B 2010-05-12 1.6 The roles of glutamates and metal ions in a rationally designed nitric oxide reductase based on myoglobin: Zn(II)-I107E FeBMb (Zn(II) binding to FeB site)
1N9I 2003-06-10 1.6 structure of earth-grown oxidized myoglobin mutant YQR (ISS8A)
1MZ0 2003-07-29 1.6 STRUCTURE OF MYOGLOBIN MB-YQR 316 ns AFTER PHOTOLYSIS OF CARBON MONOXIDE SOLVED FROM LAUE DATA AT RT.
5B84 2016-08-24 1.61 X-ray crystal structure of met I107Y sperm whale myoglobin
6CF0 2018-05-02 1.64 Sperm Whale Myoglobin H64V Mutant with Nitrite
4TYX 2014-08-13 1.64 Structure of aquoferric sperm whale myoglobin L29H/F33Y/F43H/S92A mutant
1YOG 1996-12-07 1.65 COBALT MYOGLOBIN (DEOXY)
3MN0 2010-08-11 1.65 Introducing a 2-His-1-Glu Non-Heme Iron Center into Myoglobin confers Nitric Oxide Reductase activity: Cu(II)-CN-FeBMb(-His) form
1YOI 1996-12-07 1.65 COBALT MYOGLOBIN (OXY)
3M39 2010-05-12 1.65 The roles of glutamates and metal ions in a rationally designed nitric oxide reductase based on myoglobin: Fe(II)-I107E FeBMb (Fe(II) binding to FeB site)
2EB8 2007-07-03 1.65 Crystal Structure of Cu(II)(Sal-Phe)/apo-Myoglobin
1YOH 1996-12-07 1.65 COBALT MYOGLOBIN (MET)
3NML 2010-07-14 1.68 Sperm whale myoglobin mutant H64W carbonmonoxy-form
2BW9 2005-07-13 1.68 Laue Structure of L29W MbCO
2MYE 1994-01-31 1.68 HIGH RESOLUTION X-RAY STRUCTURES OF MYOGLOBIN-AND HEMOGLOBIN-ALKYL ISOCYANIDE COMPLEXES
1AJH 1997-11-12 1.69 PHOTOPRODUCT OF CARBONMONOXY MYOGLOBIN AT 40 K
1AJG 1997-11-12 1.69 CARBONMONOXY MYOGLOBIN AT 40 K
1JPB 2002-01-16 1.7 Sperm Whale met-Myoglobin (low temperature; high pressure)
2SPL 1994-01-31 1.7 A NOVEL SITE-DIRECTED MUTANT OF MYOGLOBIN WITH AN UNUSUALLY HIGH O2 AFFINITY AND LOW AUTOOXIDATION RATE
1LTW 1996-12-23 1.7 RECOMBINANT SPERM WHALE MYOGLOBIN 29W MUTANT (OXY)
1VXE 1996-08-01 1.7 NATIVE SPERM WHALE MYOGLOBIN
2SPM 1994-01-31 1.7 A NOVEL SITE-DIRECTED MUTANT OF MYOGLOBIN WITH AN UNUSUALLY HIGH O2 AFFINITY AND LOW AUTOOXIDATION RATE
1LUE 2003-11-04 1.7 RECOMBINANT SPERM WHALE MYOGLOBIN H64D/V68A/D122N MUTANT (MET)
2SPO 1994-01-31 1.7 A NOVEL SITE-DIRECTED MUTANT OF MYOGLOBIN WITH AN UNUSUALLY HIGH O2 AFFINITY AND LOW AUTOOXIDATION RATE
1CIK 1999-04-09 1.7 RECOMBINANT SPERM WHALE MYOGLOBIN I99A MUTANT (MET)
1VXG 1996-08-01 1.7 NATIVE SPERM WHALE MYOGLOBIN
2MGE 1994-01-31 1.7 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
1DO4 2000-01-05 1.7 CARBONMONOXY-MYOGLOBIN (MUTANT L29W) AFTER PHOTOLYSIS AT T<180K
1MCY 1995-12-07 1.7 SPERM WHALE MYOGLOBIN (MUTANT WITH INITIATOR MET AND WITH HIS 64 REPLACED BY GLN, LEU 29 REPLACED BY PHE
1DTI 2000-01-30 1.7 Recombinant sperm whale myoglobin h97d, d122n mutant (met)
1F65 2000-07-19 1.7 CRYSTAL STRUCTURE OF OXY SPERM WHALE MYOGLOBIN MUTANT Y(B10)Q(E7)R(E10)
5ILM 2016-09-21 1.7 H64A sperm whale myoglobin with a Fe-chlorophenyl moiety
5XKW 2018-05-16 1.7 myoglobin mutant F43Y/F46Y
1VXD 1996-08-01 1.7 NATIVE SPERM WHALE MYOGLOBIN
1VXC 1996-08-01 1.7 NATIVE SPERM WHALE MYOGLOBIN
1MYM 1994-01-31 1.7 STRUCTURAL DETERMINANTS OF CO STRETCHING VIBRATION FREQUENCIES IN MYOGLOBIN
1MTJ 1995-09-15 1.7 PHE46(CD4) ORIENTS THE DISTAL HISTIDINE FOR HYDROGEN BONDING TO BOUND LIGANDS IN SPERM WHALE MYOGLOBIN
2SPN 1994-01-31 1.7 A NOVEL SITE-DIRECTED MUTANT OF MYOGLOBIN WITH AN UNUSUALLY HIGH O2 AFFINITY AND LOW AUTOOXIDATION RATE
3H57 2009-05-05 1.7 Myoglobin Cavity Mutant H64LV68N Deoxy form
5VZN 2018-06-06 1.7 Wild-type sperm whale myoglobin with nitric oxide
5KD1 2017-05-10 1.7 Sperm whale myoglobin H64A with nitrosoamphetamine
1JP9 2002-01-16 1.7 Sperm Whale met-Myoglobin (low temperature; high pressure)
1VXH 1996-08-01 1.7 NATIVE SPERM WHALE MYOGLOBIN
2BLI 2005-04-06 1.7 L29W Mb deoxy
1HJT 1997-11-12 1.7 SPERM WHALE MYOGLOBIN (FERROUS, NITRIC OXIDE BOUND)
1VXF 1996-08-01 1.7 NATIVE SPERM WHALE MYOGLOBIN
1MLS 1994-08-31 1.7 Structural and functional effects of apolar mutations of val68(e11) in myoglobin
2EVP 2006-03-28 1.7 The Structures of Thiolate- and Carboxylate-Ligated Ferric H93G Myoglobin: Models for Cytochrome P450 and for Oxyanion-Bound Heme Proteins
1MLL 1994-08-31 1.7 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
5KKK 2017-02-15 1.7 1.7-Angstrom In situ Mylar structure of sperm whale myoglobin (SWMb-CO) at 100 K
1TES 1996-11-08 1.7 OXYGEN BINDING MUSCLE PROTEIN
104M 1998-04-08 1.71 SPERM WHALE MYOGLOBIN N-BUTYL ISOCYANIDE AT PH 7.0
3K9Z 2009-12-01 1.72 Rational Design of a Structural and Functional Nitric Oxide Reductase
2W6X 2009-12-22 1.73 Crystal structure of Sperm Whale Myoglobin mutant YQRF in complex with Xenon
3ASE 2011-04-27 1.75 Crystal Structure of Zinc myoglobin soaked with Ru3O cluster
3A2G 2010-03-02 1.75 Crystal Structure of K102C-Myoglobin conjugated with Fluorescein
6E02 2019-07-17 1.76 WT swMb-MeNO
6E03 2019-07-17 1.76 sperm whale myoglobin nitrosoethane adduct
5ZEO 2018-05-09 1.77 X-ray structure of sperm whale V21C/V66C/F46S myoglobin mutant with an intramolecular disulfide bond
2BLH 2005-04-06 1.77 Ligand Migration and Protein Fluctuations in Myoglobin Mutant L29W
110M 1998-04-08 1.77 SPERM WHALE MYOGLOBIN D122N METHYL ISOCYANIDE AT PH 9.0
5ILE 2016-09-21 1.77 H64A sperm whale myoglobin with a Fe-tolyl moiety
5YZF 2018-03-07 1.77 X-ray crystal structure of met K42C sperm whale myoglobin
6D45 2018-05-23 1.78 L89S Mutant of FeBMb Sperm Whale Myoglobin
5UTD 2018-02-28 1.78 Sperm whale myoglobin V68A/I107Y with nitrite
5UT8 2018-02-28 1.78 Deoxy form of sperm whale myoglobin H64A
5VZP 2018-06-06 1.78 Sperm whale myoglobin H64Q with nitric oxide
5UTB 2018-02-28 1.78 Met form of sperm whale myoglobin V68A/I107Y
5VZO 2018-06-06 1.78 Sperm whale myoglobin H64A with nitric oxide
5C6Y 2015-11-11 1.79 A sperm whale myoglobin double mutant L29H/F43Y Mb with a Tyr-heme cross-link
5VZQ 2018-06-06 1.79 Sperm whale myoglobin V68A/I107Y with nitric oxide
1N9F 2003-06-10 1.8 Structure of earth-grown oxidized Myoglobin mutant YQR (ISS6A)
1OFK 1998-11-11 1.8 RECOMBINANT SPERM WHALE MYOGLOBIN F43H, H64L MUTANT (MET)
3H58 2009-05-05 1.8 Myoglobin Cavity Mutant H64LV68N Met form
2MB5 1991-04-15 1.8 HYDRATION IN PROTEIN CRYSTALS. A NEUTRON DIFFRACTION ANALYSIS OF CARBONMONOXYMYOGLOBIN
5M3S 2016-12-14 1.8 Low-dose fixed target serial synchrotron crystallography structure of Metmyoglobin
2OHB 2007-01-23 1.8 Myoglobin cavity mutant I107W
5ZZG 2018-10-03 1.8 X-ray structure of F43Y/H64D sperm whale myoglobin in complex with TCP
1F63 2000-07-19 1.8 CRYSTAL STRUCTURE OF DEOXY SPERM WHALE MYOGLOBIN MUTANT Y(B10)Q(E7)R(E10)
1MTK 1995-09-15 1.8 PHE46(CD4) ORIENTS THE DISTAL HISTIDINE FOR HYDROGEN BONDING TO BOUND LIGANDS IN SPERM WHALE MYOGLOBIN
1CO8 1999-06-14 1.8 RECOMBINANT SPERM WHALE MYOGLOBIN L104A MUTANT (MET)
5UTC 2018-02-28 1.8 Deoxy form of sperm whale myoglobin V68A/I107Y
1MLM 1994-08-31 1.8 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
2MGF 1994-01-31 1.8 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
2EB9 2007-07-03 1.8 Crystal Structure of Cu(II)(Sal-Leu)/apo-Myoglobin
2CMM 1994-01-31 1.8 STRUCTURAL ANALYSIS OF THE MYOGLOBIN RECONSTITUTED WITH IRON PORPHINE
1CH9 1999-04-09 1.8 RECOMBINANT SPERM WHALE MYOGLOBIN H97Q MUTANT (MET)
1MLO 1994-08-31 1.8 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
2OHA 2007-01-23 1.8 Myoglobin cavity mutant F138W
4FWY 2012-07-18 1.8 F33Y CuB myoglobin (F33Y L29H F43H sperm whale myoglobin) with copper bound
2BLJ 2005-04-06 1.8 Structure of L29W MbCO
2MGD 1994-01-31 1.8 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
2MYC 1994-01-31 1.8 HIGH RESOLUTION X-RAY STRUCTURES OF MYOGLOBIN-AND HEMOGLOBIN-ALKYL ISOCYANIDE COMPLEXES
1SWM 1994-01-31 1.8 X-RAY CRYSTAL STRUCTURE OF THE FERRIC SPERM WHALE MYOGLOBIN: IMIDAZOLE COMPLEX AT 2.0 ANGSTROMS RESOLUTION
2MGG 1994-01-31 1.8 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
2MYD 1994-01-31 1.8 HIGH RESOLUTION X-RAY STRUCTURES OF MYOGLOBIN-AND HEMOGLOBIN-ALKYL ISOCYANIDE COMPLEXES
2OH9 2007-01-23 1.8 Myoglobin cavity mutant V68W
1EBC 1999-08-13 1.8 SPERM WHALE MET-MYOGLOBIN:CYANIDE COMPLEX
2OH8 2007-01-23 1.8 Myoglobin cavity mutant I28W
1CH2 1999-04-09 1.8 RECOMBINANT SPERM WHALE MYOGLOBIN L89F MUTANT (MET)
1N9H 2003-06-10 1.8 structure of microgravity-grown oxidized myoglobin mutant YQR (ISS6A)
5O41 2017-06-14 1.8 Low-dose fixed target serial synchrotron crystallography structure of sperm whale myoglobin
1OFJ 1998-05-27 1.8 RECOMBINANT SPERM WHALE MYOGLOBIN L29H/H64L/D122N MUTANT (WITH INITIATOR MET)
1MLK 1994-08-31 1.8 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
2MYA 1994-01-31 1.8 HIGH RESOLUTION X-RAY STRUCTURES OF MYOGLOBIN-AND HEMOGLOBIN-ALKYL ISOCYANIDE COMPLEXES
2EF2 2007-10-02 1.8 Crystal Structure of an Artificial Metalloprotein:Rh(Phebox-Ph)/apo-A71G Myoglobin
6F18 2018-08-22 1.8 Structure of Mb NMH H64V, V68A mutant complex with EDA
5UTA 2018-02-28 1.81 Sperm whale myoglobin H64Q with nitrite
109M 1998-04-08 1.83 SPERM WHALE MYOGLOBIN D122N ETHYL ISOCYANIDE AT PH 9.0
102M 1998-04-08 1.84 SPERM WHALE MYOGLOBIN H64A AQUOMET AT PH 9.0
1OBM 1998-04-08 1.85 RECOMBINANT SPERM WHALE MYOGLOBIN 29F/64Q/68F/122N MUTANT (MET)
5UT7 2018-02-28 1.85 Wild-type sperm whale myoglobin with nitrite
5UT9 2018-02-28 1.85 Sperm whale myoglobin H64A with nitrite
5B85 2016-08-10 1.85 X-ray structure of ferric F138Y sperm whale myoglobin
1DO7 2000-01-05 1.85 CARBONMONOXY-MYOGLOBIN (MUTANT L29W) REBINDING STRUCTURE AFTER PHOTOLYSIS AT T< 180K
5ILR 2016-09-21 1.87 H64Q sperm whale myoglobin with a Fe-chlorophenyl moiety
5IKS 2016-09-21 1.87 Wild-type sperm whale myoglobin with a Fe-phenyl moiety
111M 1998-04-08 1.88 SPERM WHALE MYOGLOBIN D122N N-BUTYL ISOCYANIDE AT PH 9.0
5ILP 2016-09-21 1.88 H64Q sperm whale myoglobin with a Fe-tolyl moiety
6BMG 2017-11-29 1.88 Structure of Recombinant Dwarf Sperm Whale Myoglobin (Oxy)
6F19 2018-08-22 1.89 Structure of Mb NMH H64V, V68A mutant complex with EDA incubated at room temperature for 5 min
4FWZ 2012-07-18 1.9 Aquoferric CuB myoglobin (L29H F43H sperm whale myoglobin)
2G0S 2006-07-04 1.9 Unphotolyzed CO-bound L29F Myoglobin, crystal 2
2MYB 1994-01-31 1.9 HIGH RESOLUTION X-RAY STRUCTURES OF MYOGLOBIN-AND HEMOGLOBIN-ALKYL ISOCYANIDE COMPLEXES
1IOP 1998-04-08 1.9 INCORPORATION OF A HEMIN WITH THE SHORTEST ACID SIDE-CHAINS INTO MYOGLOBIN
2BWH 2005-07-28 1.9 Laue Structure of a Short Lived State of L29W Myoglobin
1CH7 1999-04-09 1.9 RECOMBINANT SPERM WHALE MYOGLOBIN H97F MUTANT (MET)
1MTI 1995-09-15 1.9 PHE46(CD4) ORIENTS THE DISTAL HISTIDINE FOR HYDROGEN BONDING TO BOUND LIGANDS IN SPERM WHALE MYOGLOBIN
2G10 2006-07-04 1.9 Photolyzed CO L29F Myoglobin: 3.16ns
4FWX 2012-07-18 1.9 Aquoferric F33Y CuB myoglobin (F33Y L29H F43H sperm whale myoglobin)
2G14 2006-07-04 1.9 Photolyzed CO L29F Myoglobin: 3.16us
1MLU 1994-08-31 1.9 NITRIC OXIDE RECOMBINATION TO DOUBLE MUTANTS OF MYOGLOBIN: THE ROLE OF LIGAND DIFFUSION IN A FLUCTUATING HEME POCKET
1CH1 1999-04-09 1.9 Recombinant sperm whale myoglobin L89G mutatnt (MET)
2MGC 1994-01-31 1.9 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
5JOM 2016-08-17 1.9 X-ray structure of CO-bound sperm whale myoglobin using a fixed target crystallography chip
2G11 2006-07-04 1.9 Photolyzed CO L29F Myoglobin: 31.6ns
1MOA 1995-02-07 1.9 A NOVEL SITE-DIRECTED MUTANT OF MYOGLOBIN WITH AN UNUSUALLY HIGH O2 AFFINITY AND LOW AUTOOXIDATION RATE
1J52 2003-11-11 1.9 Recombinant sperm whale myoglobin in the presence of 7atm xenon
1JDO 1998-05-27 1.9 SPERM WHALE MYOGLOBIN (FERROUS, NITRIC OXIDE BOUND)
2MGM 1994-01-31 1.9 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
1MGN 1994-01-31 1.9 HIS64(E7)-> TYR APOMYOGLOBIN AS A REAGENT FOR MEASURING RATES OF HEMIN DISSOCIATION
2G12 2006-07-04 1.9 Photolyzed CO L29F Myoglobin: 316ns
4PQB 2015-01-14 1.94 A sperm whale myoglobin double mutant L29E/F43H Mb with a non-native bis-His (His64/His93) coordination
2G0R 2006-07-04 1.95 Unphotolyzed CO-bound L29F Myoglobin
2G0Z 2006-07-04 1.95 Photolyzed CO L29F Myoglobin: 1ns
2G0X 2006-07-04 1.95 Photolyzed CO L29F Myoglobin: 316ps
1O16 2003-11-04 1.95 RECOMBINANT SPERM WHALE MYOGLOBIN H64D/V68S/D122N MUTANT (MET)
2G0V 2006-07-04 1.95 Photolyzed CO L29F Myoglobin: 100ps
6MV0 2019-03-27 1.97 CO-bound Sperm Whale Myoglobin, room temperature structure solved by serial 5degree oscillation crystallography
5YCI 2018-09-19 1.97 Ancestral myoglobin aMbWb' of Basilosaurus relative (polyphyly)
106M 1998-04-08 1.99 SPERM WHALE MYOGLOBIN V68F ETHYL ISOCYANIDE AT PH 9.0
6JP1 2019-05-29 1.99 An X-ray structure of met sperm whale F43Y/T67R myoglobin with Tyr-heme double cross-links
2W6W 2009-04-28 1.99 Crystal structure of recombinant Sperm Whale Myoglobin under 1atm of Xenon
2MGH 1994-01-31 2.0 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
1MLR 1994-08-31 2.0 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
2MGL 1994-01-31 2.0 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
5WJK 2017-12-13 2.0 2.0-Angstrom In situ Mylar structure of sperm whale myoglobin (SWMb) at 293 K
1MLH 1994-08-31 2.0 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
1MLQ 1995-02-07 2.0 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
1MLN 1994-08-31 2.0 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
2MGK 1994-01-31 2.0 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
1MLF 1994-08-31 2.0 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
1MOD 1995-02-07 2.0 HIGH-RESOLUTION CRYSTAL STRUCTURES OF DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
2MGI 1994-01-31 2.0 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
1CP0 1999-06-14 2.0 RECOMBINANT SPERM WHALE MYOGLOBIN L104N MUTANT (MET)
1SPE 1996-03-08 2.0 SPERM WHALE NATIVE CO MYOGLOBIN AT PH 4.0, TEMP 4C
6N02 2019-03-27 2.0 CO-bound Sperm Whale Myoglobin, room temperature structure, first 2 degrees of 5 degree total oscillation
1CQ2 1999-08-16 2.0 NEUTRON STRUCTURE OF FULLY DEUTERATED SPERM WHALE MYOGLOBIN AT 2.0 ANGSTROM
1MLJ 1994-08-31 2.0 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
2MGB 1994-01-31 2.0 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
1MOC 1995-02-07 2.0 HIGH-RESOLUTION CRYSTAL STRUCTURES OF DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
1MLG 1994-08-31 2.0 STRUCTURAL AND FUNCTIONAL EFFECTS OF APOLAR MUTATIONS OF VAL68(E11) IN MYOGLOBIN
6E04 2019-07-17 2.0 sperm whale myoglobin 1-nitrosopropane
1CH3 1999-04-09 2.0 RECOMBINANT SPERM WHALE MYOGLOBIN L89W MUTANT (MET)
1MBN 1976-05-19 2.0 The stereochemistry of the protein myoglobin
1MBI 1991-10-15 2.0 X-RAY CRYSTAL STRUCTURE OF THE FERRIC SPERM WHALE MYOGLOBIN: IMIDAZOLE COMPLEX AT 2.0 ANGSTROMS RESOLUTION
2MGJ 1994-01-31 2.0 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
1VXB 1996-08-01 2.0 NATIVE SPERM WHALE MYOGLOBIN
1DUO 2000-02-07 2.0 SPERM WHALE METAQUOMYOGLOBIN PROXIMAL HISTIDINE MUTANT H93G WITH 1-METHYLIMIDAZOLE AS PROXIMAL LIGAND.
5MBN 1989-07-12 2.0 REFINEMENT OF MYOGLOBIN AND CYTOCHROME C
4MBN 1989-07-12 2.0 REFINEMENT OF MYOGLOBIN AND CYTOCHROME C
1VXA 1996-08-01 2.0 NATIVE SPERM WHALE MYOGLOBIN
5VRT 2017-08-16 2.0 Nonheme Iron Replacement in a Biosynthetic Nitric Oxide Reductase Model Performing O2 Reduction to Water: Co-bound FeBMb
105M 1998-04-08 2.02 SPERM WHALE MYOGLOBIN N-BUTYL ISOCYANIDE AT PH 9.0
103M 1998-04-08 2.07 SPERM WHALE MYOGLOBIN H64A N-BUTYL ISOCYANIDE AT PH 9.0
101M 1998-04-08 2.07 SPERM WHALE MYOGLOBIN F46V N-BUTYL ISOCYANIDE AT PH 9.0
107M 1998-04-08 2.09 SPERM WHALE MYOGLOBIN V68F N-BUTYL ISOCYANIDE AT PH 9.0
6N03 2019-03-27 2.1 CO-bound Sperm Whale Myoglobin, room temperature structure, last 2 degrees of 5 degree total oscillation and 160 kGy dose
1CP5 1999-06-14 2.1 RECOMBINANT SPERM WHALE MYOGLOBIN L104F MUTANT (MET)
1CH5 1999-04-09 2.1 RECOMBINANT SPERM WHALE MYOGLOBIN H97V MUTANT (MET)
1UFP 2004-05-18 2.1 Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-wild type Myoglobin
1DUK 2000-02-07 2.13 WILD-TYPE RECOMBINANT SPERM WHALE METAQUOMYOGLOBIN
1DTM 2000-02-07 2.13 CRYSTAL STRUCTURE OF THE SPERM-WHALE MYOGLOBIN MUTANT H93G COMPLEXED WITH 4-METHYLIMIDAZOLE, METAQUO FORM
1IRC 1996-07-11 2.17 CYSTEINE RICH INTESTINAL PROTEIN
1CPW 1999-06-14 2.2 RECOMBINANT SPERM WHALE MYOGLOBIN L104W MUTANT (MET)
1MOB 1995-02-07 2.2 HIGH-RESOLUTION CRYSTAL STRUCTURES OF DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
2MGA 1994-01-31 2.2 HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN
2D6C 2006-10-31 2.26 Crystal structure of myoglobin reconstituted with iron porphycene
1JP6 2002-01-16 2.3 Sperm Whale met-Myoglobin (room temperature; room pressure)
1JP8 2002-01-16 2.3 Sperm Whale met-Myoglobin (room temperature; high pressure)
112M 1998-04-08 2.34 SPERM WHALE MYOGLOBIN D122N N-PROPYL ISOCYANIDE AT PH 9.0
6F1A 2018-08-22 2.4 Structure of Mb NMH H64V, V68A mutant complex with EDA incubated at room temperature for 20 min
108M 1998-05-20 2.67 SPERM WHALE MYOGLOBIN V68F N-BUTYL ISOCYANIDE AT PH 7.0
1F6H 2000-07-21 3.31 COMBINED RIETVELD AND STEREOCHEMICAL RESTRAINT REFINEMENT OF A PROTEIN

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
90.3 Myoglobin P02180 MYG_BALPH
90.8 Myoglobin P68278 MYG_PHOPH
90.8 Myoglobin P68277 MYG_PHODA
90.8 Myoglobin P68279 MYG_TURTR
90.8 Myoglobin P68276 MYG_DELDE
90.8 Myoglobin Q0KIY7 MYG1_STEAT
91.4 Myoglobin P02173 MYG_ORCOR
90.9 Myoglobin P02179 MYG_BALAC
92.1 Myoglobin P02174 MYG_GLOME
91.4 Myoglobin P02181 MYG_INIGE
91.4 Myoglobin Q0KIY3 MYG_PENEL
92.2 Myoglobin P02178 MYG_MEGNO
92.9 Myoglobin P02177 MYG_ESCRO
92.9 Myoglobin Q0KIY2 MYG_BALED
92.9 Myoglobin Q0KIY1 MYG_BALBO
96.8 Myoglobin P02184 MYG_KOGSI
96.8 Myoglobin Q0KIY5 MYG_KOGBR
100.0 Myoglobin P02185 MYG_PHYMC
90.1 Myoglobin P02182 MYG_ZIPCA
90.1 Myoglobin Q0KIY0 MYG_MESST
90.1 Myoglobin P02183 MYG_MESCA