Mutation of the conserved Asp122 in the linker impedes creatine kinase reactivation and refolding.


Abstract

Creatine kinase (CK), a key enzyme in maintaining the intracellular energetic homeostasis, contains two domains connected by a long linker. In this research,we found that the mutations of the conserved Asp122 in the linker slightly affected CK activity, structure and stability. The hydrogen bonding and the ion pair contributed 2-5 kJ/mol to the conformational stability of CK. Interestingly, the ability of CK reactivation from the denatured state was completely removed by the mutations. These results suggested that the electrostatic interactions were crucial to the action of the linker in CK reactivation. Study holds ProTherm entries: 25282, 25283, 25284, 25285, 25286, 25287, 25288, 25289, 25290, 25291, 25292, 25293 Extra Details: Creatine kinase reactivation; Linker; Electrostatic interactions

Submission Details

ID: YDQSuSPn

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:55 p.m.

Version: 1

Publication Details
Liu YM;Feng S;Ding XL;Kang CF;Yan YB,Int. J. Biol. Macromol. (2009) Mutation of the conserved Asp122 in the linker impedes creatine kinase reactivation and refolding. PMID:19263506
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1U6R 2005-08-02 1.65 Transition state analog complex of muscle creatine kinase (R134K) mutant
1G0W 2001-02-07 2.3 CRYSTAL STRUCTURE OF BOVINE RETINAL CREATINE KINASE
2CRK 1999-01-13 2.35 MUSCLE CREATINE KINASE
1I0E 2003-04-01 3.5 CRYSTAL STRUCTURE OF CREATINE KINASE FROM HUMAN MUSCLE

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
91.3 Creatine kinase M-type P00565 KCRM_CHICK
96.6 Creatine kinase M-type P06732 KCRM_HUMAN
96.9 Creatine kinase M-type P05123 KCRM_CANLF
97.4 Creatine kinase M-type Q9XSC6 KCRM_BOVIN
97.4 Creatine kinase M-type Q5XLD3 KCRM_PIG
97.9 Creatine kinase M-type P00564 KCRM_RAT
97.9 Creatine kinase M-type P07310 KCRM_MOUSE
100.0 Creatine kinase M-type P00563 KCRM_RABIT