Semirational design of active tumor suppressor p53 DNA binding domain with enhanced stability.


Abstract

We have designed a p53 DNA binding domain that has virtually the same binding affinity for the gadd45 promoter as does wild-type protein but is considerably more stable. The design strategy was based on molecular evolution of the protein domain. Naturally occurring amino acid substitutions were identified by comparing the sequences of p53 homologues from 23 species, introducing them into wild-type human p53, and measuring the changes in stability. The most stable substitutions were combined in a multiple mutant. The advantage of this strategy is that, by substituting with naturally occurring residues, the function is likely to be unimpaired. All point mutants bind the consensus DNA sequence. The changes in stability ranged from +1.27 (less stable Q165K) to -1.49 (more stable N239Y) kcal mol-1, respectively. The changes in free energy of unfolding on mutation are additive. Of interest, the two most stable mutants (N239Y and N268D) have been known to act as suppressors and restored the activity of two of the most common tumorigenic mutants. Of the 20 single mutants, 10 are cancer-associated, though their frequency of occurrence is extremely low: A129D, Q165K, Q167E, and D148E are less stable and M133L, V203A and N239Y are more stable whereas the rest are neutral. The quadruple mutant (M133LV203AN239YN268D), which is stabilized by 2.65 kcal mol-1 and Tm raised by 5.6 degreesC is of potential interest for trials in vivo.

Submission Details

ID: WjJC3h6P

Submitter: Shu-Ching Ou

Submission Date: Nov. 21, 2018, 2:44 p.m.

Version: 1

Publication Details
Nikolova PV;Henckel J;Lane DP;Fersht AR,Proc Natl Acad Sci U S A (1998) Semirational design of active tumor suppressor p53 DNA binding domain with enhanced stability. PMID:9843948
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1OLH 1995-03-31 HIGH-RESOLUTION SOLUTION STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF P53 BY MULTI-DIMENSIONAL NMR
1DT7 2000-07-26 SOLUTION STRUCTURE OF THE C-TERMINAL NEGATIVE REGULATORY DOMAIN OF P53 IN A COMPLEX WITH CA2+-BOUND S100B(BB)
1SAE 1995-10-15 HIGH RESOLUTION SOLUTION NMR STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF P53 BY MULTI-DIMENSIONAL NMR (SAC STRUCTURES)
1HS5 2001-01-10 NMR SOLUTION STRUCTURE OF DESIGNED P53 DIMER
2J10 2007-08-28 p53 tetramerization domain mutant T329F Q331K
1SAK 1995-10-15 HIGH RESOLUTION SOLUTION NMR STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF P53 BY MULTI-DIMENSIONAL NMR (SAC STRUCTURES)
1PET 1995-02-07 NMR SOLUTION STRUCTURE OF THE TETRAMERIC MINIMUM TRANSFORMING DOMAIN OF P53
2GS0 2006-10-31 NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and the activation domain of p53
5HOU 2016-03-16 Solution Structure of p53TAD-TAZ1
2L14 2010-11-03 Structure of CBP nuclear coactivator binding domain in complex with p53 TAD
2MZD 2015-03-25 Characterization of the p300 Taz2-p53 TAD2 Complex and Comparison with the p300 Taz2-p53 TAD1 Complex
2MWP 2014-12-10 Solution structure of 53BP1 tandem Tudor domains in complex with a p53K382me2 peptide
1OLG 1995-01-26 HIGH-RESOLUTION SOLUTION STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF P53 BY MULTI-DIMENSIONAL NMR
5HPD 2016-03-16 Solution Structure of TAZ2-p53TAD
2MEJ 2014-04-30 Solution Structure of the Complex Between BCL-xL and the p53 Core Domain determined with PRE restraints
2J0Z 2007-08-28 p53 tetramerization domain wild type
2J11 2007-08-28 p53 tetramerization domain mutant Y327S T329G Q331G
3SAK 1999-06-25 HIGH RESOLUTION SOLUTION NMR STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF P53 BY MULTI-DIMENSIONAL NMR (SAC STRUCTURES)
1SAF 1995-10-15 HIGH RESOLUTION SOLUTION NMR STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF P53 BY MULTI-DIMENSIONAL NMR (SAD STRUCTURES)
2LY4 2012-10-31 HMGB1-facilitated p53 DNA binding occurs via HMG-box/p53 transactivation domain interaction and is regulated by the acidic tail
2FEJ 2006-01-31 Solution structure of human p53 DNA binding domain.
5HP0 2016-03-16 Solution Structure of TAZ2-p53AD2
1A1U 1998-04-08 SOLUTION STRUCTURE DETERMINATION OF A P53 MUTANT DIMERIZATION DOMAIN, NMR, MINIMIZED AVERAGE STRUCTURE
2MWO 2014-12-10 Solution structure of 53BP1 tandem Tudor domains in complex with a p53K370me2 peptide
2MWY 2016-01-27 Mdmx-p53
1SAL 1995-10-15 HIGH RESOLUTION SOLUTION NMR STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF P53 BY MULTI-DIMENSIONAL NMR (SAD STRUCTURES)
1PES 1995-02-07 NMR SOLUTION STRUCTURE OF THE TETRAMERIC MINIMUM TRANSFORMING DOMAIN OF P53
1JSP 2002-08-17 NMR Structure of CBP Bromodomain in complex with p53 peptide
2K8F 2009-03-03 Structural Basis for the Regulation of p53 Function by p300
2RUK 2014-10-15 Solution structure of the complex between p53 transactivation domain 2 and TFIIH p62 PH domain
5MHC 2017-10-04 1.2 Crystal structure of 14-3-3sigma and a p53 C-terminal 12-mer synthetic phosphopeptide
3D06 2009-01-20 1.2 Human p53 core domain with hot spot mutation R249S (I)
6GGC 2019-05-22 1.24 p53 cancer mutant Y220C in complex with small-molecule stabilizer PK9320
6SHZ 2020-02-19 1.24 p53 cancer mutant Y220C
4MZI 2014-01-15 1.25 Crystal structure of a human mutant p53
6GGE 2019-05-22 1.25 p53 cancer mutant Y220C in complex with small-molecule stabilizer PK9327
3LW1 2010-03-23 1.28 Binary complex of 14-3-3 sigma and p53 pT387-peptide
6RL3 2020-06-17 1.3 Fragment AZ-003 binding at the p53pT387/14-3-3 sigma interface
5O1E 2018-05-09 1.3 p53 cancer mutant Y220C im complex with compound MB577
5O1C 2018-05-09 1.32 p53 cancer mutant Y220C in complex with compound MB184
5O1H 2018-05-09 1.32 p53 cancer mutant Y220C in complex with compound MB539
6GGB 2019-05-22 1.32 p53 cancer mutant Y220C in complex with small-molecule stabilizer PK9318
6GGF 2019-05-22 1.32 Structure of the p53 cancer mutant Y220C in complex with small-molecule stabilizer PK9328
5AOK 2015-12-16 1.35 Structure of the p53 cancer mutant Y220C with bound small molecule PhiKan7099
5G4N 2016-06-22 1.35 Crystal structure of the p53 cancer mutant Y220C in complex with a difluorinated derivative of the small molecule stabilizer Phikan083
5O1G 2018-05-09 1.35 p53 cancer mutant Y220C in complex with compound MB487
3ZME 2013-05-08 1.35 Structure of the p53 core domain mutant Y220C bound to the small molecule PhiKan7242
6V4F 2020-04-22 1.35 Crystal Structure Analysis of Zebra Fish MDMX
5O1D 2018-05-09 1.36 p53 cancer mutant Y220C in complex with compound MB481
5AB9 2015-12-16 1.36 Structure of the p53 cancer mutant Y220C with bound small molecule 7- ethyl-3-(piperidin-4-yl)-1H-indole
5O1F 2018-05-09 1.38 p53 cancer mutant Y220C in complex with compound MB582
5G4M 2016-06-22 1.38 Crystal structure of the p53 cancer mutant Y220C in complex with a monofluorinated derivative of the small molecule stabilizer Phikan083
5A7B 2015-09-30 1.4 Structure of the p53 cancer Y220C bound to the stabilizing small molecule PhiKan5211
6SI3 2020-02-19 1.4 p53 cancer mutant Y220S in complex with small-molecule stabilizer PK9301
5O1I 2018-05-09 1.4 p53 cancer mutant Y220C in complex with compound MB710
3D08 2009-01-20 1.4 Human p53 core domain with hot spot mutation R249S and second-site suppressor mutation H168R
6GGD 2019-05-22 1.4 p53 cancer mutant Y220C in complex with small-molecule stabilizer PK9324
1YC5 2005-04-26 1.4 Sir2-p53 peptide-nicotinamide
5LAP 2016-08-10 1.42 p53 cancer mutant Y220C with Cys182 alkylation
4AGQ 2012-03-21 1.42 Structure of the p53 core domain mutant Y220C bound to the stabilizing small molecule PhiKan5196
5O1B 2018-05-09 1.43 p53 cancer mutant Y220C in complex with compound MB84
5O1A 2018-05-09 1.44 p53 cancer mutant Y220C in complex with compound MB240
6SI1 2020-02-19 1.44 p53 cancer mutant Y220H
4AGO 2012-03-21 1.45 Structure of the p53 core domain mutant Y220C bound to the stabilizing small molecule PhiKan5174
5MG7 2018-06-13 1.45 New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-p53R2)
5MCT 2018-06-13 1.45 New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-LHG1)
4IBY 2013-08-14 1.45 Human p53 core domain with hot spot mutation R273H and second-site suppressor mutation S240R
6RWU 2020-06-17 1.46 Fragment AZ-010 binding at the p53pT387/14-3-3 sigma interface
5AOJ 2015-12-16 1.47 Structure of the p53 cancer mutant Y220C in complex with 2-hydroxy-3, 5-diiodo-4-(1H-pyrrol-1-yl)benzoic acid
5G4O 2016-06-22 1.48 Crystal structure of the p53 cancer mutant Y220C in complex with a trifluorinated derivative of the small molecule stabilizer Phikan083
6SI2 2020-02-19 1.5 p53 cancer mutant Y220S
1AIE 1997-06-16 1.5 P53 TETRAMERIZATION DOMAIN CRYSTAL STRUCTURE
2VUK 2008-07-22 1.5 Structure of the p53 core domain mutant Y220C bound to the stabilizing small-molecule drug PhiKan083
4AGP 2012-03-21 1.5 Structure of the p53 core domain mutant Y220C bound to the stabilizing small molecule PhiKan5176
5AOL 2015-12-16 1.5 Structure of the p53 cancer mutant Y220C with bound 3-bromo-5-(trifluoromethyl)benzene-1,2-diamine
4KVP 2013-07-31 1.5 Human p53 Core Domain Mutant V157F
4AGM 2012-03-21 1.52 Structure of the p53 core domain mutant Y220C bound to the stabilizing small molecule PhiKan5086
6SI0 2020-02-19 1.53 p53 cancer mutant Y220C in complex with small-molecule stabilizer PK9323
6V4H 2020-04-22 1.53 Crystal Structure Analysis of Zebra Fish MDMX
6RWS 2020-06-17 1.53 Fragment AZ-009 binding at the p53pT387/14-3-3 sigma interface
2PCX 2008-04-08 1.54 Crystal structure of p53DBD(R282Q) at 1.54-angstrom Resolution
6GGA 2019-05-22 1.55 p53 cancer mutant Y220C in complex with small-molecule stabilizer PK9284
6RJZ 2020-06-17 1.58 Fragment AZ-015 binding at the p53pT387/14-3-3 sigma interface
4RP7 2016-01-13 1.58 Structure of the amyloid-forming segment TIITLE from p53 (residues 253-258)
5MF7 2018-05-30 1.59 New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-GADD45)
6SIO 2020-06-17 1.6 Fragment AZ-017 binding at the p53pT387/14-3-3 sigma interface
4AGN 2012-03-21 1.6 Structure of the p53 core domain mutant Y220C bound to the stabilizing small molecule PhiKan5116
2B3G 2005-10-11 1.6 p53N (fragment 33-60) bound to RPA70N
6RL4 2020-06-17 1.6 Fragment AZ-025 binding at the p53pT387/14-3-3 sigma interface
6SIP 2020-06-17 1.6 Fragment AZ-011 binding at the p53pT387/14-3-3 sigma interface
5MCV 2018-06-13 1.6 New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-LWC1)
6SIQ 2020-06-17 1.6 Fragment AZ-012 binding at the p53pT387/14-3-3 sigma interface
2FOJ 2006-02-14 1.6 The Crystal Structure of the N-terminal domain of HAUSP/USP7 complexed with p53 peptide 364-367
2X0U 2010-01-26 1.6 STRUCTURE OF THE P53 CORE DOMAIN MUTANT Y220C BOUND TO A 2-amino substituted benzothiazole scaffold
6RL6 2020-06-17 1.6 Fragment AZ-024 binding at the p53pT387/14-3-3 sigma interface
2J21 2006-09-20 1.6 Human p53 core domain mutant M133L-V203A-N239Y-N268D-R282W
6RK8 2020-06-17 1.6 Fragment AZ-014 binding at the p53pT387/14-3-3 sigma interface
6RM7 2020-06-17 1.6 Fragment AZ-026 binding at the p53pT387/14-3-3 sigma interface
5ABA 2015-12-16 1.62 Structure of the p53 cancer mutant Y220C with bound small-molecule stabilizer PhiKan5149
6SIN 2020-06-17 1.64 Fragment AZ-020 binding at the p53pT387/14-3-3 sigma interface
6RWI 2020-06-17 1.65 Fragment AZ-002 binding at the p53pT387/14-3-3 sigma interface
2J1X 2006-09-20 1.65 Human p53 core domain mutant M133L-V203A-Y220C-N239Y-N268D
6SL6 2020-03-11 1.67 p53 charged core
2XWR 2011-03-30 1.68 Crystal structure of the DNA-binding domain of human p53 with extended N terminus
6RWH 2020-06-17 1.68 Fragment AZ-007 binding at a primary and secondary binding site of the the p53pT387/14-3-3 sigma complex
2J1Y 2006-09-20 1.69 Human p53 core domain mutant M133L-V203A-N239Y-G245S-N268D
6S9Q 2020-06-17 1.69 Fragment AZ-004 binding at a primary and secondary site in a p53pT387/14-3-3 complex
4IBT 2013-08-14 1.7 Human p53 core domain with hot spot mutation R273H and second-site suppressor mutation T284R
4AGL 2012-03-21 1.7 Structure of the p53 core domain mutant Y220C bound to the stabilizing small molecule PhiKan784
2H2D 2006-09-19 1.7 The Structural Basis for Sirtuin Substrate Affinity
5MCU 2018-06-13 1.7 New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-LHG2)
1C26 1999-07-27 1.7 CRYSTAL STRUCTURE OF P53 TETRAMERIZATION DOMAIN
3IGK 2010-03-31 1.7 Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs (p53-DNA complex 2)
4RP6 2016-01-13 1.7 Structure of the amyloid-forming segment LTIITLE from p53 (residues 252-258)
3D05 2009-01-20 1.7 Human p53 core domain with hot spot mutation R249S (II)
4IBU 2013-08-14 1.7 Human p53 core domain with hot spot mutation R273C and second-site suppressor mutation T284R in sequence-specific complex with DNA
5AOM 2015-12-16 1.74 Structure of the p53 cancer mutant Y220C with bound small molecule PhiKan883
2WGX 2009-05-12 1.75 HUMAN P53 CORE DOMAIN MUTANT M133L-V203A-Y236F-N239Y-T253I-N268D
1XQH 2004-11-23 1.75 Crystal structure of a ternary complex of the methyltransferase SET9 (also known as SET7/9) with a P53 peptide and SAH
4IJT 2013-08-14 1.78 Human p53 core domain with hot spot mutation R273H (form II)
4IBS 2013-08-14 1.78 Human p53 core domain with hot spot mutation R273H (form I)
5AOI 2015-12-16 1.78 Structure of the p53 cancer mutant Y220C in complex with an indole- based small molecule
4IBW 2013-08-14 1.79 Human p53 core domain with hot spot mutation R273H and second-site suppressor mutation T284R in sequence-specific complex with DNA
2J20 2006-09-20 1.8 Human p53 core domain mutant M133L-V203A-N239Y-N268D-R273C
2J1Z 2006-09-20 1.8 Human p53 core domain mutant M133L-V203A-N239Y-N268D-F270L
3IGL 2010-03-31 1.8 Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs (p53-DNA complex 1)
3DAC 2008-09-02 1.8 Structure of the human Mdmx protein bound to the p53 tumor suppressor transactivation domain
3D0A 2009-01-20 1.8 Human p53 core domain with hot spot mutation R249S and second site suppressor mutation H168R in sequence-specific complex with DNA
4IBQ 2013-08-14 1.8 Human p53 core domain with hot spot mutation R273C
2BIQ 2005-01-26 1.8 human p53 core domain mutant T123A-M133L-H168R-V203A-N239Y-R249S- N268D
2X0V 2010-01-26 1.8 STRUCTURE OF THE P53 CORE DOMAIN MUTANT Y220C BOUND TO 4-(trifluoromethyl)benzene-1,2-diamine
4X34 2015-03-04 1.8 Crystal structure of the 53BP1 tandem tudor domain in complex with p53K381acK382me2
6SI4 2020-02-19 1.8 p53 cancer mutant Y220S in complex with small-molecule stabilizer PK9323
5MOC 2017-10-04 1.8 Crystal structure of 14-3-3sigma and a p53 C-terminal 12-mer synthetic phosphopeptide
3PDH 2011-01-19 1.8 Structure of Sir2Tm bound to a propionylated peptide
2J1W 2006-09-20 1.8 Human p53 core domain mutant M133L-V143A-V203A-N239Y-N268D
2AC0 2006-07-11 1.8 Structural Basis of DNA Recognition by p53 Tetramers (complex I)
2BIP 2005-01-26 1.8 human p53 core domain mutant M133L-H168R-V203A-N239Y-R249S-N268D
5BUA 2016-07-06 1.81 Lysine 120-acetylated P53 DNA binding domain in a complex with DNA.
6RX2 2020-06-17 1.82 Fragment AZ-005 binding at the p53pT387/14-3-3 sigma interface
4LOE 2013-07-31 1.85 Human p53 Core Domain Mutant N239Y
2AHI 2006-07-11 1.85 Structural Basis of DNA Recognition by p53 Tetramers (complex III)
6RKK 2020-06-17 1.88 Fragment AZ-021 binding at the p53pT387/14-3-3 sigma interface
6S39 2020-06-17 1.88 Fragment AZ-018 binding at the p53pT387/14-3-3 sigma interface
6R5L 2020-06-17 1.88 Fragment AZ-006 binding at the p53pT387/14-3-3 sigma interface
6RKM 2020-06-17 1.88 Fragment AZ-022 binding at the p53pT387/14-3-3 sigma interface
6RKI 2020-06-17 1.88 Fragment AZ-023 binding at the p53pT387/14-3-3 sigma interface
6RM5 2020-06-17 1.88 Fragment AZ-016 binding at the p53pT387/14-3-3 sigma interface
3DAB 2008-09-02 1.9 Structure of the human Mdmx protein bound to the p53 tumor suppressor transactivation domain
2BIO 2005-01-26 1.9 human p53 core domain mutant M133L-V203A-N239Y-R249S-N268D
5MCW 2018-06-13 1.9 New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-LWC2)
6S40 2020-06-17 1.9 Fragment AZ-001 binding at the p53pT387/14-3-3 sigma interface and additional sites
3D09 2009-01-20 1.9 Human p53 core domain with hot spot mutation R249S and second-site suppressor mutations H168R and T123A
1UOL 2003-10-16 1.9 Crystal structure of the human p53 core domain mutant M133L/V203A/N239Y/N268D at 1.9 A resolution.
6SLV 2020-06-17 1.9 Fragment AZ-013 binding at the p53pT387/14-3-3 sigma interface
2YBG 2011-05-04 1.9 Structure of Lys120-acetylated p53 core domain
4BUZ 2013-07-17 1.9 SIR2 COMPLEX STRUCTURE MIXTURE OF EX-527 INHIBITOR AND REACTION PRODUCTS OR OF REACTION SUBSTRATES P53 PEPTIDE AND NAD
2H59 2006-09-05 1.9 Sir2 H116A-deacetylated p53 peptide-3'-o-acetyl ADP ribose
2BIN 2005-01-26 1.9 human p53 core domain mutant M133L-H168R-V203A-N239Y-N268D
4HJE 2013-07-17 1.91 Crystal structure of p53 core domain in complex with DNA
3KZ8 2010-03-31 1.91 Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs (p53-DNA complex 3)
4QO1 2014-10-29 1.92 p53 DNA binding domain in complex with Nb139
4IBZ 2013-08-14 1.92 Human p53 core domain with hot spot mutation R273C and second-site suppressor mutation T284R
2BIM 2005-01-26 1.98 human p53 core domain mutant M133L-V203A-N239Y-N268D-R273H
2H4H 2006-09-05 1.99 Sir2 H116Y mutant-p53 peptide-NAD
5OL0 2018-02-28 1.99 Structure of Leishmania infantum Silent Information Regulator 2 related protein 1 (LiSIR2rp1) in complex with acetylated p53 peptide
5XZC 2019-01-23 10.7 Cryo-EM structure of p300-p53 protein complex
6FF9 2018-04-25 2.0 Mutant R280K of human P53
1MA3 2002-10-16 2.0 Structure of a Sir2 enzyme bound to an acetylated p53 peptide
2V5W 2007-09-04 2.0 Crystal structure of HDAC8-substrate complex
6S3C 2020-06-17 2.0 Fragment AZ-019 binding at the p53pT387/14-3-3 sigma interface
4LOF 2013-07-31 2.0 Human p53 Core Domain Mutant V157F/N235K/N239Y
2H4F 2006-09-05 2.0 Sir2-p53 peptide-NAD+
2OCJ 2007-03-06 2.05 Human p53 core domain in the absence of DNA
6FJ5 2018-06-27 2.05 New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-AGG-HG)
2H4J 2006-09-05 2.1 Sir2-deacetylated peptide (from enzymatic turnover in crystal)
2X0W 2010-01-26 2.1 STRUCTURE OF THE P53 CORE DOMAIN MUTANT Y220C BOUND TO 5,6-dimethoxy- 2-methylbenzothiazole
4FZ3 2013-03-20 2.1 Crystal structure of SIRT3 in complex with acetyl p53 peptide coupled with 4-amino-7-methylcoumarin
3Q01 2011-05-11 2.1 An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity
4IBV 2013-08-14 2.1 Human p53 core domain with hot spot mutation R273C and second-site suppressor mutation S240R in sequence-specific complex with DNA
5UN8 2017-03-15 2.13 Crystal Structure of human O-GlcNAcase in complex with glycopeptide p53
3KMD 2010-02-23 2.15 Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer
2H2F 2006-12-05 2.2 The Structural basis for Sirtuin Substrate affinity
3D07 2009-01-20 2.2 Human p53 core domain with hot spot mutation R249S (III)
1TSR 1996-01-29 2.2 P53 CORE DOMAIN IN COMPLEX WITH DNA
2ATA 2006-07-11 2.2 Structural Basis of DNA Recognition by p53 Tetramers (complex II)
1TUP 1995-07-11 2.2 TUMOR SUPPRESSOR P53 COMPLEXED WITH DNA
2FOO 2006-02-14 2.2 The Crystal Structure of the N-terminal domain of HAUSP/USP7 complexed with p53 peptide 359-362
1YCS 1997-11-19 2.2 P53-53BP2 COMPLEX
1H26 2003-02-01 2.24 CDK2/CyclinA in complex with an 11-residue recruitment peptide from p53
4XR8 2016-02-03 2.25 Crystal structure of the HPV16 E6/E6AP/p53 ternary complex at 2.25 A resolution
2Z5S 2007-11-06 2.3 Molecular basis for the inhibition of p53 by Mdmx
1YCQ 1997-11-19 2.3 XENOPUS LAEVIS MDM2 BOUND TO THE TRANSACTIVATION DOMAIN OF HUMAN P53
3TG5 2011-08-31 2.3 Structure of SMYD2 in complex with p53 and SAH
2Z5T 2007-11-06 2.3 Molecular basis for the inhibition of p53 by Mdmx
6VR1 2020-06-10 2.37 Complex of HLA-A2, a class I MHC, with a p53 peptide
6VR5 2020-06-17 2.38 Complex of HLA-A2, a class I MHC, with a p53 peptide
3Q05 2011-05-11 2.4 An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity
7BWN 2020-06-17 2.4 Crystal Structure of a Designed Protein Heterocatenane
6VRN 2020-06-17 2.46 T cell receptor-p53-HLA-A2 complex
3OQ5 2010-09-22 2.5 Crystal structure of the 3-MBT domain from human L3MBTL1 in complex with p53K382me1
1KZY 2002-03-20 2.5 Crystal Structure of the 53bp1 BRCT Region Complexed to Tumor Suppressor P53
2ADY 2006-07-11 2.5 Structural Basis of DNA Recognition by p53 Tetramers (complex IV)
4LO9 2013-07-31 2.5 Human p53 Core Domain Mutant N235K
1GZH 2002-06-27 2.6 Crystal structure of the BRCT domains of human 53BP1 bound to the p53 tumor supressor
1YCR 1997-11-19 2.6 MDM2 BOUND TO THE TRANSACTIVATION DOMAIN OF P53
6VRM 2020-06-17 2.61 T cell receptor-p53-HLA-A2 complex
4HFZ 2013-07-31 2.69 Crystal Structure of an MDM2/P53 Peptide Complex
4ZZJ 2015-07-15 2.74 SIRT1/Activator/Substrate Complex
2YDR 2012-03-14 2.75 CpOGA D298N in complex with p53-derived O-GlcNAc peptide
3TS8 2011-11-23 2.8 Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element
4MZR 2014-01-15 2.9 Crystal structure of a polypeptide p53 mutant bound to DNA
5LGY 2016-07-27 2.92 Lysine 120-acetylated P53 DNA binding domain in a complex with the BAX response element.
5ECG 2015-12-16 3.0 Crystal structure of the BRCT domains of 53BP1 in complex with p53 and H2AX-pSer139 (gammaH2AX)
6VQO 2020-06-17 3.0 T cell receptor-p53-HLA-A2 complex
6T58 2020-05-27 3.1 Structure determination of the transactivation domain of p53 in complex with S100A4 using annexin A2 as a crystallization chaperone
2H1L 2006-09-12 3.16 The Structure of the Oncoprotein SV40 Large T Antigen and p53 Tumor Suppressor Complex
3Q06 2011-05-11 3.2 An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity
4BV2 2013-07-17 3.3 CRYSTAL STRUCTURE OF SIR2 IN COMPLEX WITH THE INHIBITOR EX-527, 2'-O-ACETYL-ADP-RIBOSE AND DEACETYLATED P53-PEPTIDE
6RZ3 2019-10-30 4.23 Crystal structure of a complex between the DNA-binding domain of p53 and the carboxyl-terminal conserved region of iASPP

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
93.1 Cellular tumor antigen p53 Q9TTA1 P53_TUPBE
95.7 Cellular tumor antigen p53 P56424 P53_MACMU
95.7 Cellular tumor antigen p53 P61260 P53_MACFU
95.7 Cellular tumor antigen p53 P56423 P53_MACFA
95.2 Cellular tumor antigen p53 P13481 P53_CHLAE
100.0 Cellular tumor antigen p53 P04637 P53_HUMAN